Anton Vila-sanjurjo- PhD
- Professor at Universidade da Coruña
Anton Vila-sanjurjo
- PhD
- Professor at Universidade da Coruña
Professor in Genetics
About
60
Publications
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2,421
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Introduction
Current institution
Additional affiliations
January 2022 - present
October 2013 - present
Universidade da Coruña (UDC)
Position
- Project PI
September 2011 - October 2013
Publications
Publications (60)
Given the local and systemic adverse reactions associated with whole cell pertussis vaccines combined with diphtheria and tetanus toxoids (DTP), acellular pertussis vaccines combined with the same toxoids (DTaP) were developed in the 1990s. In comparison to DTP, DTaP vaccines demonstrated reduced reactogenicity and equivalent or improved immunogeni...
Here, we introduce the highly versatile circular polymerase chain reaction (CiPCR) technique, propose a mechanism of action, and describe a number of examples demonstrating the versatility of this technique. CiPCR takes place between two fragments of dsDNA with two homologous regions, as long as one of the fragments carries said regions at its 3′-...
A new species of Mycena from northwestern Spain is described, illustrated and morphologically and molecularly compared to related species. The new species belongs to M. sect. Viscipelles, presenting as tiny in stature, with small spores, claviform to subglobose cheilocystidia with short excrescences, and a small disc at the base of the stipe, which...
Altered mito-ribosomal fidelity is an important and insufficiently understood causative agent of mitochondrial dysfunction. Its pathogenic effects are particularly well-known in the case of mitochondrially induced deafness, due to the existence of the, so called, ototoxic variants at positions 847C (m.1494C) and 908A (m.1555A) of 12S mitochondrial...
The last few years have witnessed dramatic advances in our understanding of the structure and function of the mammalian mito-ribosome. At the same time, the first attempts to elucidate the effects of mito-ribosomal fidelity (decoding accuracy) in disease have been made. Hence, the time is right to push an important frontier in our understanding of...
El género Mycena (Pers.) Roussel está ampliamente distribuido por todo el mundo (Kirk et al. 2008), comprende 1.312 especies aceptadas (Kalichman et al. 2020) siendo el tercer género más numeroso dentro del Orden Agaricales. Es un género polifilético (Moncalvo 2000; Oliveira 2021) y se hacen necesarios análisis moleculares para aclarar su compleja...
Here we summarize our latest efforts to elucidate the role of mtDNA variantsMtDNA variants affecting the mitochondrial translation machinery, namely variants mapping to the mt-rRNA and mt-tRNA genes. Evidence is accumulating to suggest that the cellular response to interference with mitochondrial translation is different from that occurring as a re...
In our efforts to improve the quality and stability of chitosan nanoparticles (NPs), we describe here a new type of chitosan NPs dually crosslinked with genipin and sodium tripolyphosphate (TPP) that display quorum quenching activity. These NPs were created using a simplified and robust procedure that resulted in improved physicochemical properties...
Functioning mitochondria are crucial for cancer metabolism, but aerobic glycolysis is still considered to be an important pathway for energy production in many tumor cells. Here we show that two well established, classic Hodgkin lymphoma cell lines (cHL) harbor deleterious variants within mitochondrial DNA (mtDNA) and thus exhibit reduced steady st...
Minority Gene Expression Profiling (MGEP) refers to a scenario where the expression profiles of specific genes of interest are concentrated in a small cellular pool that is embedded within a larger, non-expressive pool. An example of this is the analysis of disease-related genes within sub-populations of blood or biopsied tissues. These systems are...
This brief report serves as an introduction to a supplement of the Journal of Infectious Diseases entitled "Next-Generation Sequencing (NGS) Technologies to Advance Global Infectious Disease Research." We briefly discuss the history of NGS technologies and describe how the techniques developed during the past 40 years have impacted our understandin...
Photobacteriosis caused by Photobacterium damselae subsp. piscicida (Pdp) remains one of the main infectious diseases affecting cultured fish in Mediterranean countries. Diverse vaccine formulations based in the use of inactivated bacterial cells have been used with unsatisfactory results, especially in newly cultured species like sole (Solea seneg...
We have fabricated two types of crosslinked chitosan-based nanoparticles (NPs), namely (1) ionically crosslinked with tripolyphosphate (TPP), designated as IC-NPs and (2) dually co-crosslinked (ionically and covalently with TPP and genipin, respectively) termed CC-NPs. The two types of NPs were physichochemically characterized by means of DLS-NIBS,...
Diet may be modified seasonally or by biogeographic, demographic or cultural shifts. It can differentially influence mitochondrial bioenergetics, retrograde signalling to the nuclear genome, and anterograde signalling to mitochondria. All these interactions have the potential to alter the frequencies of mtDNA haplotypes (mitotypes) in nature and ma...
Population cage studies.
(A) Illustration of the steps for all population cage studies (~850 flies/cage). Eggs and then squashed flies were introduced into bottles. The squashed flies contained gut microbes and standardised the microbiome each generation. Flies developed in the bottles and the stopper was removed so flies could randomly mate in the...
Oxygen consumption rate.
(A) Activity of complex IV (n = 5 rep/mitotype/diet). (B) Activity of complex V (n = 6 rep/mitotype on the 1:2 P:C diet, and n = 7 rep/mitotype on the 1:16 P:C diet). (C) Oxygen consumption rate of extracted mitochondria with succinate as the substrate (n = 6 biological rep/mitotype/diet) did not differ significantly betwee...
RNA-seq results for Dahomey vs Alstonville with FDR< 0.05 (A) 1:2 P:C diet (B) 1:16 P:C diet. Positive fold change indicates up-regulated in Dahomey, while negative fold change indicates up-regulated in Alstonville.
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Additional data used in the generation of figures in the manuscript and supporting information.
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Microbiome in larval guts.
The proportion of bacteria was influenced by mitotype when larvae were fed the 1:16 P:C food but not the 1:2 P:C diet (n = 6 biological rep/mitotype/diet for the 1:2 P:C diet and n = 9 biological rep/mitotype/diet for the 1:16 P:C diet, see text).
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Quaternary structural modelling.
(A) The Drosophila supercomplex showing the predicted movement of protons in yellow. The V161L ND4 mutation is circled in yellow and the D40N COIII mutation in orange. (B) COIII mutation showing the site of mutation in orange circle and structurally related residues. The mutation site does not appear to interact wit...
Paraquat treatment assay.
Alstonville larvae treated with paraquat produced a phenocopy of the Dahomey control. ANOVA of the effects on development showed a significant effect of paraquat treatment (F1, 32 = 22.97, p< 0.0001) but no significant effect of mitotype or diet (F1, 32 = 2.67, p = 0.11, F1, 32 = 0, p = 1, respectively). In regards to the...
KEGG pathways differentially expressed between mitotypes according to RNA-seq profiling (P< 0.01).
(A) Alstonville up-regulated/Dahomey down-regulated when larvae are fed the 1:2 P:C food. (B) Alstonville down-regulated/Dahomey up-regulated when larvae are fed the 1:2 P:C food. (C) Dahomey up-regulated/Alstonville down-regulated when larvae are fed...
Glucose-6-phosphate dehydrogenase (G6PD) activity.
Activity was determined spectrophotometrically from the rate of reduction of NADP (n = 8 biological rep/mitotype). Bars (mean ± s.e.m). * p< 0.05, as calculated by t-tests (see text).
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Differences between the mitochondrial genomes of the Dahomey, Madang, Alstonville and Victoria Falls fly mitotypes.
Position is taken from the alignment of GenBank No’s KP843845, KP843849, KP843842 and KP843854, respectively accessed on 12 April 2018. Syn is synonymous, Nonsyn is nonsynonymous, ItSpace is intervening spacer region and Con is consen...
Rotenone treatment assays showing grouping.
(A) Adding rotenone to the Alstonville diet created a Dahomey phenocopy. This phenocopy developed more quickly than Alstonville controls when fed the 1:16 P:C food showing that partial inhibition of complex I was beneficial. Adding rotenone to the Dahomey fly food created a disease model and these larvae...
Top 5 Gene Ontology terms comparing Dahomey with Alstonville on the 1:2 P:C and 1:16 P:C diets.
(A) Alstonville up-regulated/Dahomey down-regulated when larvae are fed the 1:2 P:C food. (B) Alstonville down-regulated/Dahomey up-regulated when larvae are fed the 1:2 P:C food. (C) Dahomey up-regulated/Alstonville down-regulated when larvae are fed th...
Differentially expressed metabolites from whole third instar female wandering larvae as assessed by GC/MS for the (A) 1:2 P:C diet and (B) 1:16 P:C diet. Values are false discovery rates (FDR). (+) Indicates up-regulated in Dahomey while (-) indicates up-regulated in Alstonville. Peak area is relative to Alstonville larvae. Peak area value is mean±...
RNA-seq and RT-qPCR results for Dahomey vs Alstonville.
(A) 1:2 P:C diet (B) The 1:16 P:C diet. Values are false discovery rates (FDR) as determined by moderated t-tests for RNA-seq or t-test for RT-qPCR. (+) Indicates up-regulated in Dahomey while (-) indicates up-regulated in Alstonville. MtDNA genes were: rRNA is ribosomal ribonucleic acid, ATP6...
Differentially expressed mitochondrial ribosomal proteins in larvae fed the 1:16 P:C diet.
Value are false discovery rates (FDR) as determined by moderated t-tests for RNA-seq. (+) Indicates up-regulated in Dahomey while (-) indicates up-regulated in Alstonville.
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Connexins (Cxs) are integral membrane proteins that form high-conductance plasma membrane channels, allowing communication from cell to cell (via gap junctions) and from cells to the extracellular environment (via hemichannels). Initially described for their role in joining excitable cells (nerve and muscle), gap junctions (GJs) are found between v...
Quorum sensing (QS) explains a type of bacterial cell-cell communication mediated by exocellular compounds that act as autoinducers (AIs). As such, QS can be considered the most primordial form of language. QS has profound implications for the control of many important traits ( e.g. biofilm formation, secretion of virulence factors, etc.). Conceptu...
Background
Connexins (Cxs) are integral membrane proteins that form plasma membrane channels, allowing cell-matrix and cell to cell communication. Initially described in joining excitable cells (nerve and muscle), gap junctions (GJs) are found joining virtually all cells in solid tissues and are essential for the functional co-ordination of organs...
Supplementary material.
Mitochondrial DNA mutations are well recognized as an important cause of disease, with over two hundred variants in the protein encoding and mt-tRNA genes associated with human disorders. In contrast, the two genes encoding the mitochondrial rRNAs (mt-rRNAs) have been studied in far less detail. This is because establishing the pathogenicity of mt-...
Despite the identification of a large number of potentially pathogenic variants in the mitochondrially encoded rRNA (mt-rRNA) genes, we lack direct methods to firmly establish their pathogenicity. In the absence of such methods, we have devised an indirect approach named heterologous inferential analysis or HIA that can be used to make predictions...
Mutations of mitochondrial DNA are linked to many human diseases. Despite the identification of a large number of variants in the mitochondrially-encoded rRNA (mt-rRNA) genes, the evidence supporting their pathogenicity is, at best, circumstantial. Establishing the pathogenicity of these variations is of major diagnostic importance. Here, we aim to...
The A-minor interaction, formed between single-stranded adenosines and the minor groove of a receptor helix, is among the most common motifs found in rRNA. Among the A-minors found in 16S rRNA are a set of interactions between adenosines at positions 1433, 1434 and 1468 in helix 44 (h44) and their receptors in the nucleotide 320-340 region of helix...
The small and large subunits of the ribosome are held together by a series of bridges, involving RNA–RNA, RNA–protein and
protein–protein interactions. Some 12 bridges have been described for the Escherichia coli 70S ribosome. In this work, we have targeted for mutagenesis, some of the 16S rRNA residues involved in the formation of
intersubunit bri...
Bacterial communities are important not only in the cycling of organic compounds but also in maintaining ecosystems. Specific
bacterial groups can be affected as a result of changes in environmental conditions caused by human activities, such as agricultural
practices. The aim of this study was to analyze the effects of different forms of tillage a...
It is well known that agricultural practices change the physical and chemical characteristics of soil. As a result, microbial
populations can also be affected. The aim of this study was to analyze the effect on soil bacterial communities of zero tillage
(ZT) under maize monoculture (MM) with crop residue removal (-R) (MM/-R treatment), compared to...
Ribosomal RNAs (rRNAs) from all kingdoms contain a variety of post-transcriptional modifications and these are typically clustered in the functional centers of the ribosome. The functions of two bases in the 23S rRNA of Escherichia coli that are post-transcriptionally modified, m5U1939 and [psi]2504, were examined by mutagenesis of the rRNA bases a...
Modification of the Ribosome and the Translational Machinery during Reduced Growth Due to Environmental Stress, Page 1 of 2
Abstract
Escherichia coli strains normally used under laboratory conditions have been selected for maximum growth rates and require maximum translation efficiency. Recent studies have shed light on the structural and functio...
Ribosomal protein S12 is a critical component of the decoding center of the 30S ribosomal subunit and is involved in both tRNA selection and the response to streptomycin. We have investigated the interplay between S12 and some of the surrounding 16S rRNA residues by examining the phenotypes of double-mutant ribosomes in strains of Escherichia coli...
The prokaryotic ribosome is an important target of antibiotic action. We determined the X-ray structure of the aminoglycoside kasugamycin (Ksg) in complex with the Escherichia coli 70S ribosome at 3.5-A resolution. The structure reveals that the drug binds within the messenger RNA channel of the 30S subunit between the universally conserved G926 an...
We describe two structures of the intact bacterial ribosome from Escherichia coli determined to a resolution of 3.5 angstroms by x-ray crystallography. These structures provide a detailed view of the interface
between the small and large ribosomal subunits and the conformation of the peptidyl transferase center in the context of the
intact ribosome...
During environmental stress, organisms limit protein synthesis by storing inactive ribosomes that are rapidly reactivated when conditions improve. Here we present structural and biochemical data showing that protein Y, an Escherichia coli stress protein, fills the tRNA- and mRNA-binding channel of the small ribosomal subunit to stabilize intact rib...
Protein biosynthesis on the ribosome requires accurate reading of the genetic code in mRNA. Two conformational rearrangements in the small ribosomal subunit, a closing of the head and body around the incoming tRNA and an RNA helical switch near the mRNA decoding site, have been proposed to select for complementary base-pairing between mRNA codons a...
Interactions within the decoding center of the 30 S ribosomal subunit have been investigated by constructing all 15 possible mutations at nucleotides C1402 and A1500 in helix 44 of 16 S rRNA. As expected, most of the mutations resulted in highly deleterious phenotypes, consistent with the high degree of conservation of this region and its functiona...
Three ribosomal RNA mutations conferring resistance to the antibiotic kasugamycin were isolated using a strain of Escherichia coli in which all of the rRNA is transcribed from a plasmid-encoded rrn operon. The mutations, A794G, G926A, and A1519C, mapped to universally conserved sites in the 16 S RNA gene. Site-directed mutagenesis studies showed th...
Phylogenetic comparison of rRNA sequences has suggested that a pseudoknot structure exists in the central domain of small-subunit rRNA. In Escherichia coli 16S rRNA, this pseudoknot would form when positions 570 and 571 pair with positions 865 and 866. Mutations were introduced into this pseudoknot at the phylogenetically invariant nucleotides U571...
Questions
Questions (3)
I'd like to isolate mtDNA from wild mushrooms but I cannot find any protocol to do so in the publicly available literature. Can anybody provide a protocol for the purification of mtDNA from carpophores?
Hi,
I was wondering what the ratio of mitoribosomes to cytoplasmic ribosomes in mammalian cells might be. I am searching online for papers that could address this issue but it is quite hard to find any information. Anyone knows of any paper in which this ratio is reported?
Antón
We observe a decrease in GFP fluorescence in a culture of bacterial cells treated with certain nanoparticles? The decrease could either be due to an inhibition of the biological process being studied (desired effect) or to quenching of GFP fluorescence by the nanoparticles (undesired effect). The absorbance spectrum of the nanoparticles is flat near the absorption and emission peaks of GFP. Can we rule out quenching based on this?
