Anthony Massé

Anthony Massé
University of Nantes | UNIV Nantes · Laboratoire de génie des procédés, environnement, agroalimentaire (GEPEA)

PhD Chemical Engineering

About

59
Publications
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Introduction
Coupling and integration of membrane processes for bio-production or refining of micro and macro-algae. My research aims to find the best operating conditions for: - the conduct of membrane photo-bioreactors set up to improve the growth of micro-algae and the continuous extraction of metabolites, - the recycling of micro-algae culture medium, - the separation of biomolecules as well as the concentration, purification and fractionation of compounds derived from micro or macrolagae

Publications

Publications (59)
Article
Full-text available
This study aims to investigate the bio-refinery process through an enzyme-assisted extraction (EAE) on freeze-dried and fresh macroalgae Gracilaria gracilis for the release of water-soluble components (R-phycoerythrin, proteins, and sugar). Three enzymes, cellulase, protease, and enzyme cocktail (mixture of cellulase and protease), were applied in...
Article
Full-text available
Haslea ostrearia is a marine diatom known to produce and excrete the marenine blue pigment. Its controlled, continuous and intensified cultivation remains a challenge. Thus, a submerged membrane photobioreactor (MPBR) was implemented in order to simultaneously and continuously cultivate H. ostrearia and extract marennine. The MPBR was compared with...
Article
Membrane diafiltration process was developed in order to fractionate the water soluble proteins of the supernatant obtained after Spirulina bead milling disruption and centrifugation. The obtained crude extract was filtered with 50 kDa, 150 kDa, 300 kDa or 0.2 µm membranes. Phycocyanin purified extracts were produced after filtration on the 300 kDa...
Article
Microalgae are recognized as source of proteins for nutraceuticals, food additives and bioactive peptides. Also, the exploitation of the protein fraction can contribute to reach the profitability of the microalgal biorefinery. Among the available technologies, membrane filtration is one of the most sustainable and cost-effective technique for the p...
Article
Active edible coatings from microalgal exopolysaccharides (EPS) enriched with different concentrations of red seaweed extract (RSE) (0.5, 1 and 1.5% (w/v)) were developed and their effects on shrimp quality with refrigerated storage (4 ± 1 °C) were studied over a period of 8 days. Untreated and treated shrimp were regularly evaluated bacteriologica...
Presentation
Skeletonema marinoi, a marine diatom-blooming species in temperate coastal waters, is a microalgae commonly used as food in shellfish hatcheries. Cell mortality is a key mechanism that shapes phytoplankton blooms and species dynamic in aquatic environment.Phytoplankton lysis has been associated with release of chemical compounds that have been show...
Article
Full-text available
The red microalga Porphyridium cruentum is exploited industrially for its exopolysaccharides (EPS) and pigments production. EPS produced by P. cruentum are partially released and dissolved into the surrounding environment, they can be recovered from the culture medium after removing the cells. This paper presents a parametric study of the ultrafilt...
Article
Full-text available
The Paralytic shellfish neurotoxins (PST) are of increasing interest for biomedical applications. The chemical synthesis is often complex and expensive that's why the purification by membrane filtration of PST from Alexandrium minutum dinoflagellate was investigated. Disrupted micro-alga cells by ultrasonic treatment were diafiltred to let pass tox...
Article
Full-text available
Exopolysaccharides (EPS) from cell-free Porphyridium cruentum media were concentrated then purified (diafiltration) on a 0.14 μm ceramic membrane. The influence of cross-flow velocities on filtration performances was investigated. Mean permeate fluxes equal to 49.8, 68.9 and 81.9 L h⁻¹. m⁻² were obtained during the concentration at 4 bars for respe...
Article
Active food packaging films based on crab chitosan and Spirulina extract (SE) were developed. The effects of the SE incorporation at different levels on physical (color, opacity water vapor and oxygen permeability) and mechanical (tensile strength and elongation at break) properties of chitosan films were investigated. FTIR was carried out to obser...
Technical Report
Full-text available
COSELMAR, a 4-year project financed by the Région des Pays de la Loire at 2.1 M€, officially started on the 7th January 2013. The project is coordinated by Philipp Hess (IFREMER) and Sophie Pardo (Université de Nantes-LEMNA) and falls under the Federation for Research Institut Universitaire Mer et Littoral (IUML, FR CNRS 3473)*. COSELMAR has also b...
Poster
use or membrane process for isolating biofunctional exopolysaccharides (EPS) produced from microalgae and cyanobacteria
Poster
Membrane filtration of microalga extract for recovering Polysaccharides: effect of the membrane characteristics and the ionic environment
Article
This study tested the efficiency of granular filtration using a bilayer sand filter for microalgae removal from culture dilutions ranging from 10,000 to 17,000 cells/mL. The objective is to evaluate the removal capacity of the filter without chemical coagulation. Two filter media, sand and anthracite, with mean grain sizes of 0.395 mm and 1.2 mm re...
Article
The ballast waters from ships pose a major threat to oceans, notably because of the spread of microorganisms. The present study evaluates the techno-economic feasibility of implementing the membrane process to remove microalgae from seawater to be ballasted in a single step during planktonic bloom. The optimal conditions for the microfiltration of...
Article
Full-text available
The present work investigates the fouling mechanisms of PVDF hollow fiber membrane (0.03 μm) during the dead end ultrafiltration at a fixed permeate flux (outside to inside configuration) of complex synthetic seawater composed by humic acids, alginic acids, inorganic particles and numerous salts at high concentrations. Short term ultrafiltration ex...
Chapter
Full-text available
Abstract (chapter 3): This study fostered partnership between scientists, shellfish growers, water treatment engineers, economists and regulators. The objective was to optimise land-based post-harvest treatments in such a way that oyster and mussel marketing could continue during toxic blooms of Dinophysis acuminata (DSP) and Alexandrium minutum (P...
Article
Abstract: Cooking juices are fish industry effluents with high organic and salt contents, typically 10-20 g COD.L-1 and 20-30 g salt.L-1. They contain valuable substances (mainly aromas and proteins) that can be recovered, thereby significantly lowering treatment cost and offering a commercial gain for the food industry. The use of membrane process...
Article
Full-text available
The role of natural compounds of seawater and added particles on mechanisms of membrane fouling and organic matter rejection has been investigated. Ultrafiltration (100 kDa) has been conducted in both dead-end (out/in) and tangential (in/out) modes on polysulfone hollow fibre membranes. The permeate fluxes are approximately three times higher for t...
Article
Full-text available
The present study has two main purposes: to investigate the performance of a first-stage sand media filter in a case of several reconstituted algal blooms and to better understand fouling mechanism of sand filter due to micro-algae. The retention efficiency of the filter obtained for 30,000, 50,000 and 145,000 cells/ml alga suspensions of Chlorella...
Article
Low pressure microfiltration (MF) and ultrafiltration (UF) is used as seawater pretreatment before reverse osmosis membranes for capacity that ranged from 6,700 to 140,000 m per day at Colakoglu Steel Mill (Turkey), Yu-Han (China), Kindasa (Saudi Arabia), Fukuoka (Japan), and Ad Dur (Bahrain). Among all modes of porous membrane filtration, pressure...
Article
Full-text available
In this study, a sand filter was used to remove micro-algae from seawater feeding aquaculture ponds. A lab-scale sand filter was used to filter 30,000 cells/mL of Heterocapsa triquetra suspension, a non-toxic micro-alga that has morphological and dimensional (15-20 microm) similarities with Alexandrium sp., one of the smallest toxic micro-algae in...
Article
The present study completes a previous work dedicated to the feasibility to implement, on-board ship, a direct nanofiltration process in order to treat laundry greywaters and recycle 80% to the inlets of the washing machines (Guilbaud et al., 2010). At present, the study investigates the influence of nanofiltration operating conditions on Chemical...
Article
The present study deals with the specific energetic consumption evaluation of dead-end and tangential ultrafiltration (UF) (100kDa) of salted suspensions on polysulfone membrane in regards of backwash and aeration conditions. For TMP ranging from 0.2 to 0.8bar, it was found that specific energy consumption during tangential UF was always higher tha...
Article
Full-text available
In this study, an original use of immersed microfiltration membranes (mean pore size equal to 0.2μm) is investigated for the total removal of toxic dinoflagellates from seawater. Using a membrane autopsy and fouling model approach and the use of fouling indexes (called Pore Blocking Index, Pore Constriction Index and Cake Filtration Index), three d...
Article
Tuna cooking juices contain high organic load preventing the rejection in the environment without treatment. But the effluents present an interesting fishy odour and it is worth recovering aroma compounds. In this work, two industrial tuna cooking juices were concentrated by nanofiltration. Nanofiltration performance was discussed in terms of perme...
Article
Full-text available
Greywater reuse inside buildings is a possible way to preserve water resources and face up to water scarcity. This study is focused on a technical-economic analysis of greywater treatment by a direct nanofiltration (NF) process or by a submerged membrane bioreactor (SMBR) for on-site recycling. The aim of this paper is to analyse the cost of recycl...
Article
The present work deals with the evaluation of hydrodynamic effects on the ultrafiltration of microalgae suspensions for harvesting or metabolite production by coupling photobioreactors with membrane separation processes. Two ultrafiltration systems were compared: the first was a cross flow ultrafiltration unit equipped with a flat sheet membrane an...
Article
Full-text available
Introduction et objectifs du projet La contamination des denrées d’origine marine par des toxines algales (phycotoxines) a pris depuis quelques années une importance considérable. Ce phénomène est largement lié à l’augmentation du nombre de zones géographiques touchées ainsi qu’à la diversification des espèces toxinogènes et à l’identification de...
Conference Paper
Greywater reuse inside buildings is a possible way to preserve water resource and face up to water scarcity. This study is focused on greywater treatment by a submerged membrane bioreactor (SMBR) for on-site recycling. The aim is to find satisfying operating conditions in order to achieve the best compromise between permeation fluxes and retent...
Article
Full-text available
On-site greywater recycling and reuse is one of the main ways to reduce potable water requirement in urban areas. Direct membrane filtration is a promising technology to recycle greywater on-site. This study aimed at selecting a tubular nanofiltration (NF) membrane and its operating conditions in order to treat and reuse greywater in buildings. To...
Article
Full-text available
In this study, an original use of low-energy-consuming submerged MF/UF membranes was investigated for the total removal of toxic micro-algae (Alexandrium sp.) from seawater. Experimentally, a non-toxic micro-alga (Heterocapsa triquetra) which has morphological and dimensional (15–20 µm) similarities with Alexandrium sp., one of the smallest toxic m...
Article
Full-text available
The present study deals with the feasibility to implement, on board ship, a direct nanofiltration process in order to treat laundry grey waters and recycle 80% to the inlets of the washing machines. At first, a specific methodology for real laundry grey water production was set up at the laboratory for the purpose. Characteristics of the lab-produc...
Article
On-site greywater recycling is one of the main ways of preserving water resources in urban or arid areas. This study aims to formulate model synthetic greywater (SGW) in order to evaluate and compare the performances of several recycling processes on a reproducible effluent. The formulated SGW is composed of septic effluent to provide indicators of...
Article
Separation and Purification Technology j o u r n a l h o m e p a g e : w w w . e l s e v i e r . c o m / l o c a t e / s e p p u r a b s t r a c t Grateloupia turuturu, an invasive red macro-alga, represents an unexploited important biomass. A poten-tial use of this seaweed is the production of valuable molecules, such as R-phycoerythrin. Neverthel...
Article
Full-text available
The production by enzymatic treatment of fish protein hydrolysates (FPH) is a promising route to add value to fisheries proteinic co-products (fish frames, heads etc.). Indeed, FPH possess good nutritional properties and biological activities for food and feed uses. Pressure-driven membrane separations such as ultrafiltration (UF) and nanofiltratio...
Article
The objective of this paper was to understand the detachment of multispecies biofilm caused by abrasion. By submitting a biofilm to different abrasion strengths (collision of particles), stratification of biofilm cohesion could be highlighted and related to stratification of biofilm bacterial communities using the PCR-SSCP fingerprint method. The b...
Article
The objective of this study was to use indirect methods to assess the biofilm cohesion and its vertical stratification. Biofilms were grown under low hydrodynamic strengths in two reactors, a low shear stress reactor (LSSR) or under a defined shear stress in a Couette-Taylor Reactor (CTR), using different electron donors and acceptors. The stratifi...
Chapter
Full-text available
This review reports some interesting works on the recovery and fractionation by membrane techniques of marine compounds resulting from hydrolysis of fish by-products such as proteins or peptides, lipids or fatty acids, and carbohydrates. Ultrafiltration (UF) appears as the most widely used technique for such applications. It must be noted that only...
Article
This work aims to compare biomass structure and performance of a submerged membrane bioreactor (SMBR) and an activated sludge process (ASP) treating the same domestic wastewater. The influence of the separation technique (membrane filtration or settling) and operation at high sludge-retention time (SRT) were investigated. Over the entire range of S...
Article
Sludge characteristics of a submerged membrane bioreactor (MBR) and an activated sludge process (AS) were compared, during a first phase at the same operating conditions (low MLSS and conventional SRT) and in a second phase with a high sludge retention time (SRT) in the membrane bioreactor. During the first phase, a bimodal flocs size distribution...
Article
Cette étude s’attache à décrire et analyser les interactions liant les spécificités du milieu biologique d’un bioréacteur à membranes immergées (BAMI) et les phénomènes de colmatage. Le BAMI a été comparé avec un procédé à boues activées, tous deux alimentés avec la même eau résiduaire urbaine. Les performances d’épuration des deux procédés sont co...

Projects

Project (1)
Project
Development and optimisation of refining processes for metabolites recovery Wet biomass treatments (bead milling) Lipids and pigments extraction Milking processes development