Anoop Kumar Srivastava

ICAR-Central Citrus Research Institute ( Formerly National Research Centre for Citrus) · Soil Science

'Candidatus Liberibacter asiaticus' (CLas) is a non-culturable α-proteobacterium, putatively causing Huanglongbing (HLB), a devastating disease currently threatening citrus production in India and all over the world. Here, we report the whole genome sequence of a CLas strain obtained from the leaf midrib of a HLB-infected Khasi mandarin tree in Ass...

Easily extractable glomalin-related soil protein (EEG) released from arbuscular mycorrhizal fungi exhibits many roles in soil and plants, while it is not clear whether the biostimulator alone or in combination with agronomic practices can improve soil characteristics and fruit quality in citrus. The objective of this study was to analyze the effect...

The culturable endophytic fungus Serendipita indica has many beneficial effects on plants, but whether and how it affects physiological activities and phosphorus (P) acquisition of tea seedlings at low P levels is unclear. The objective of this study was to analyze the effects of inoculation with S. indica on growth, gas exchange, chlorophyll fluor...

Walnut (Juglans regia) is an important nut tree species in the world, whereas walnut trees often face inadequate phosphorus (P) levels of soil, negatively limiting its growth and yield. Arbuscular mycorrhizal fungi (AMF) can colonize walnut roots, but whether and how AMF promotes walnut growth, physiological activities, and P acquisition is unclear...

The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera : Liviidae) is the primary vector of pathogenic bacteria disseminating Huanglongbing. Nutrients enhancement have been reported to be few optional methods to relieve Huanglongbing. However, limited studies have focused on the interactions between citrus plants and psyllid under nutrition...

Arbuscular mycorrhizal (AM) fungi and plants require sugars, especially during fruit ripening stage, while the underlying mechanism influencing sugar accumulation in response to AM fungi is still unclear. The objective of this study was to analyze the field inoculation response of three AM fungi (Acaulospora scrobiculata, Diversispora spurca, and D...

Induction Nutritional enhancement have been reported to effectively relieve infected symptoms of Huanglongbing, one of the most destructive diseases of citrus. However, few studies focused on the role of plant nutrition in citrus plant-vector (Asian citrus psyllid; Diaphorina citri Kuwayama) interactions, which is regarded as an important part to d...

The amount and time of fertilization play an important role in the quality and yield of citrus. Moreover, twice fertilization in the spring (March) and summer (July) are generally applied in citrus orchards, of which the appropriate proportion is unclear, especially in Ganzhou. Therefore, the two-year field experiment containing three proportions (...

Review manuscript describes about citrus nutrition research in India and future strategies

Complete and balanced nutrition has always been the first line of plant defense due to the direct involvement of mineral elements in plant protection. Mineral elements affect plant health directly by modulating the activity of redox enzymes or improving the plant vigor indirectly by altering root exudates, and changing microflora population dynamic...

Rhizosphere security has become a prerequisite to sustain the production by exploiting the microbiome profile of acid lime, and fortifying with microbes via rhizosphere hybridization is one effective way of deciphering the interaction between plants and microbes. The lost photosynthates from plants through root exudates creates a novel environment...

A field experiment was conducted for four seasons (2013–17) on 12-year-old bearing Nagpur mandarin (Citrus reticulata Blanco) plants budded on rough lemon rootstock (Citrus jambhiri Lush) at ICAR-Central Citrus Research Institute, Nagpur, Maharashtra. Six zinc-based treatments, viz. S100 as soil application of ZnSO4 (100 g/plant), S200 as soil appl...

Arbuscular mycorrhizal fungi secrete glomalin deposited into the soil as glomalin-related soil protein (GRSP), which possess multiple utility to benefit both soil as well as plant health. The present study aimed to assess the effects of the foliar application of an easily extractable GRSP (EE-GRSP) on the fruit quality, soil nutrients, and soil str...

Microorganisms have dynamic and complex interactions with their hosts. Diverse microbial communities residing near, on, and within the plants, called phytobiome, are an essential part of plant health and productivity. Exploiting citrus-associated microbiomes represents a scientific approach toward sustained and environment-friendly module of citrus...

Earthworms and arbuscular mycorrhizal fungi (AMF) are beneficial organisms in the soil and play an important role in improving soil fertility and plant growth. The objective of this study was to analyze the response of single versus dual inoculation of earthworms (Pheretima guillelmi) and AMF (Funneliformis mosseae) on mycorrhizal growth, root morp...

Sustainable agriculture has been consistently challenged due to the menace of changing climate, thereby, causing depletion in carrying capacity of soil vis-à-vis agronomic response of crop. Of late, biochar has turned out to be the most promising option to address these issues. The present review takes stock of the research work accrued under varie...

Rhizosphere is considered to be hotspot of plant growth promoting microorganisms. Artificially altering total microbiome of any crop through hybridizing two contrasting crop-based rhizospheres could be a way forward approach to sustain soil health support quality citrus production. We carried out an experiment to testify the effect of rhizosphere h...

There are many anthropogenic activities that have caused the accumulation of potentially toxic metals (e.g., lead, cadmium, chromium, nickel, arsenic, cobalt, and mercury) in the environment. Lead (Pb) is known as a very toxic and non-biodegradable element that has no metabolic function in living creatures. It can be easily taken up and transferred...

Diagnosis and management of nutrient constraints are the two important pillars of sound citrus fertilizer program, although its history in India is as old as cultivation of citrus. Of the different diagnostic tools (leaf nutrient standards, soil thresholds of soil available nutrients, metalloenzyme activity, inflorescence analysis, juice nutrient s...

The study was carried out during 2015-16 at district Sriganganagar under semi arid region of Rajasthan India with the aim to diagnose the soil fertility indicator by using DRIS norms to improve the kinnow orchards efficiency. A total 105 kinnow mandarin orchards were selected through the preliminary survey, all the selected orchards were divided eq...

Diagnosis and management of nutrient constraints are the two important pillars of sound citrus fertilizer program, although its history in India is as old as cultivation of citrus. Of the different diagnostic tools (leaf nutrient standards, soil thresholds of soil available nutrients, metalloenzyme activity, inflorescence analysis, juice nutrient s...

Huanglongbing (HLB) is one of the most complex and destructive diseases of citrus, which has left a great negative impact on growth of world citrus industry. The characteristic symptoms of HLB include leaves with blotchy mottles and stunted growth of infected plants. These symptoms are often confused with the symptoms of nutritional deficiencies. M...

Limited availability of water and nutrients in soil affects the yield and longevity of citrus plantation. Conservation of rainfall runoff not only enhances water availability but improves the nutritional status in soil which might increase the orchard efficiency in citrus. Keeping this in view, a long term (7 years) experiment was imposed to study...

Glomalin, one of the glycoproteins generated in the spores and hyphae of arbuscular mycorrhizal (AM) fungi, has multiple functions in plants and soil, while the role of foliar spray of easily extractable glomalin-related soil proteins (EE-GRSP) in citrus fruits is not well defined. Our study aimed to use referenced transcriptome sequencing to uncov...

At the present time, cadmium (Cd) and arsenic (As) contamination has become an outstanding agronomic concern. Bacterial-induced mitigation of Cd and As toxicity in plants- and bacteria-mediated immobilization of Cd and As to prevent their uptake by the crops (with no or minimum accumulation of As and Cd in edible parts) has attracted an increased a...

Chapter deals with climate resilient soil fertility management in fruit crops , using microbial consortium .

Medicinal plants are well known to have the advantages of high concentration of medicinal ingredients having clinical importance, curative value, small toxic and side effects. Important compounds viz., paclitaxel, camptothecin, and vincristine have been developed from medicinal plants as first-line of clinical drugs, leading to their consistently i...

Multiple functions of glomalin released by arbuscular mycorrhizal fungi are well-recognized, whereas the role of exogenous glomalins including easily extractable glomalin-related soil protein (EE-GRSP) and difficultly extractable glomalin-related soil protein (DE-GRSP) is unexplored for plant responses. Our study was carried out to assess the effec...

The book is a compilation of invited lectures and technical papers presented during 3rd International Conference on Global Initiatives in Agricultural, Forestry and Applied Sciences for Food Security, Environmental Safety and Sustainable Development, 17-18 October, 2021.

• Huanglongbing (HLB) causes extensive damage in citrus orchards worldwide. Symptoms include blotchy mottle leaf (BML) and little leaf chlorosis (LLC), and nutrient deficiency usually occurs concurrently. However, the relationship between plant mineral content and infection with Candidatus Liberibacter asiaticus (CLas) is not clearly established. •...

Endophytes have the ability to improve plant nutrition alongside their agronomic performance, among which arbuscular mycorrhizal fungi provide the most benefits to their host. Previously, we reported for the first time that an arbuscular mycorrhizal-like fungus Piriformospora indica had the ability to colonize roots of trifoliate orange (Poncirus t...

The role of arbuscular mycorrhizal fungi in sweet oranges is well known, but the function of their secondary metabolite, especially the easily extractable glomalin-related soil protein (EE-GRSP), an active fraction of glomalin, is still unclear. The proposed study aimed to analyze the field response of foliar application of exogenous EE-GRSP on tre...

Specialty fertilizers, completely water-soluble fertilizers with low salt index, include broad category of plant nutrient products used for managing soil health, practicing precision nutrient management, and supposedly taking care of post-harvest keeping quality. With fruits assuming centrality in the country's current thrust of achieving nutrition...

Presence and functional roles of arbuscular mycorrhizal (AM) fungi in wetland environment have gained a global importance in recent past. Wetlands build a low-oxygen atmosphere, forming a completely different ecological environment for AM fungi, known more to occur in terrestrial environment. Nevertheless, as many 101 of AM fungi species, belonging...

Doubling the farmers' income through maximising production per unit drop of water and sustaining soil health is the national goal. Horticulture sector is currently witnessing paradigm shift from 'yield maximisation' to 'enhancement of use efficiencies of irrigation water and nutrients'. Emphasis is on reducing excessive percolation of water along w...

The inoculation response of single arbuscular mycorrhiza fungi (AMF) or rhizobia (Rh) in relation to nitrogen (N) acquisition of plants is well established, while the combined effect of both AMF and Rh is poorly known with regard to N assimilation for changes in amino acids of white clover (Trifolium repens). A pot study was carried out to evaluate...

Fruit crops offer a strong sink for sequestration of atmospheric carbon dioxide, thereby, aid in moderating the impact of climate change-related issues, besides creating a parallel nutrient sink. Mere annual application of organic manures/composts has failed to address different soil fertility functions due to failure to prolong the impact of soil...

Aims: Special fertilizer and soil mulching have been used to improve crop yield worldwide. However, the effects of special fertilizer with mulching on soil characteristics of citrus orchards were not yet fully understood. This study aimed at assessing the effects of different mulch patterns including plastic mulching and grass mulching on improving...

Tea (Camellia sinensis (L.) O. Kuntze) is predominantly grown in acidic soils with severe limitations of P-nutrition. The present study evaluated the effects of an arbuscular mycorrhizal (AM) fungus, Claroideoglomus etunicatum, on root morphology, leaf and root P concentration, phosphatase activity in soils and roots, and the relative expression of...

The growing demand of quality planting material in acid lime makes the researchers to think about its sustainability in main field, maintain the soil fertility status without hampering the rhizosphere ecosystem and produce quality planting material at cheap and eco-friendly manner. Being a microbial hotspot, rhizosphere ecology performs a complex n...

Soil arbuscular mycorrhizal fungi (AMF) enhance the tolerance of plants against soil moisture deficit stress (SMDS), but the underlying mechanisms are still not fully understood. Polyamines (PAs) as low-molecular-weight, aliphatic polycations have strong roles in abiotic stress tolerance of plants. We aimed to investigate the effect of AMF (Funneli...

Different kinds of soil animals and microorganisms inhabit the plant rhizosphere, which function closely to plant roots. Of them, arbuscular mycorrhizal fungi (AMF) and earthworms play a critical role in sustaining the soilplant health. Earthworms and AMF belong to the soil community and are soil beneficial organisms at different trophic levels. Bo...

Techniques and managements of perennial fruits production are nowadays directed towards the need to conserve resources, energy and a commitment to the environment. In this sense, fertigation has risen as a valuable tool in recent years spreading around the world in major fruit belts. The million dollar question strikes why fertigation out-performs...

Rhizosphere engineering is an innovative approach to add qualitative value to plant rhizosphere to trigger improvement in crop performance without hampering the rhizosphere quality. We carried out a study addressing the agronomic performance of acid lime through microbes-mediated rhizosphere engineering in 7-year-old acid lime plant (var. NRCC acid...

Arbuscular mycorrhizal fungi (AMF) produce glomalin-related soil protein (GRSP) that influences organic carbon (C) storage in soil; however, how much purified GRSP fractions contribute to soil organic carbon (SOC) is yet not known. The present study evaluated the contribution of GRSP towards changes in SOC in trifoliate orange grown in a rootbox di...

The nutrient responsive nature of fruit crops predisposes them to multiple nutrient deficiencies, resulting in their economic manifestations on quality production is a formidable challenge to nutritionists. The crop-based soil health care is basic to any fruit nutrition program. But, still a bigger challenge lies in diagnosing the genesis of any nu...

Arbuscular mycorrhizal fungi (AMF) inhabited in citrus rhizosphere playing multiple roles in plant growth, nutrient acquisition, and stress tolerance are well known under potted conditions. however, information regarding response of AMF application in field citrus trees is scarce. In this study, single inoculation of Funneliformis mosseae and mixtu...

Climate change related issues in recent past have gripped the production sustainability of citrus worldwide. Various ways and means have been put forward to tackle the impact of climate change and eventually the derivatives of organic farming have some proven distinction. In this regard, rhizosphere security has become a pre-requisite to sustain th...

: A potted experiment was carried out to evaluate the effect of an arbuscular mycorrhizal fungus (AMF), Acaulospora scrobiculata, on peach seedlings grown in non-replant (NR) and replant (R) soils, to establish whether AMF inoculation alleviated soil replant disease through changes in physiological levels and relevant gene expression. After 15 week...

Climate change related issues in recent past have gripped the production sustainability of citrus worldwide. Various ways and means have been put forward to tackle the impact of climate change and eventually the derivatives of organic farming have some proven distinction. In this regard, rhizosphere security has become a pre-requisite to sustain th...

The combined effect of drip irrigation with fertigation on plant growth, yield and quality of 10 year-old bearing sweet orange (Citrus sinensis cv. Sathgudi) was studied during 2013-17 at Dr YSR Horticultural University, AICRP on Fruits, CRS, Tirupati. The experiment was laid out in factorial randomised block design with nine treatment combinations...

Arbuscular mycorrhizas (AMs) have the ability to enhance drought tolerance of citrus, but the underlying mechanisms have not been clearly elucidated. Considering the strong association of cell membrane fatty acid (FA) unsaturation with plant drought tolerance, the present study hypothesized that AM fungi (AMF) modulated the composition and unsatura...

Arbuscular mycorrhizal fungi (AMF) have multi-pronged utility in citrus performance, while their propagation technique is a gap. In this study, indigenous AMF in rhizosphere of Citrus unshiu grafted on trifoliate orange were isolated from fresh root segments (Φ<2 mm), fresh rhizosphere soil (< 4 mm size), and air-dried rhizosphere soil (< 4 mm size...

Sustainability in quality production and ensuring a tangible soil health, are the two most discerning challenges, which the fruit crops are likely to confront with in years to come. Integrated Nutrient Management (INM) is one such option, which exploits the synergy of divergent nutrient sources. As many as four modules of INM (T 2 : RDF + VAM 500 g...

Sustainability in quality production and ensuring a tangible soil health, are the two most discerning challenges, which the fruit crops are likely to confront with in years to come. Integrated Nutrient Management (INM) is one such option, which exploits the synergy of divergent nutrient sources. As many as four modules of INM (T2: RDF + VAM 500 g/p...

The present investigation entitled "Effect of application of value added consortium on, physico-chemical, properties of sweet orange orchard from field experiment" was conducted during the year 2014-15. In the, field experiment was laid out at Zari Ta. & Dist. Parbhani on important satellite treatments emerged out from pot culture experiment i.e.T1...

This review paper critically assesses the literature on soil-microbe-plant interactions influencing availability of micronutrients in the rhizosphere. The research entitled "Effect of rhizsophere hybridization on macro and micronutrient activities of soil" was conducted during the year 2013-14 in three phases. In the first phase, representative soi...

Importance of optimum soil fertility and plant nutrition in sustaining the citrus orchard performance is well known. Extensive surveys were carried out covering as many as 40 orchards of Khasi mandarin in Thangal village of the sub-division Nungba under Tamenglong district in the year 2010-2011. DRIS-based analysis predicted the optimum values of a...

Extensive surveys were carried out covering a many as 40 orchards of Khasi mandarin in Nungba sub-division under Tamenglong district of Manipur, India. DRIS-based analysis predicted the optimum values of available nutrients in soil as: N 142.4-222.3 %, P 5.9-8.2%, K 110.5-270.3%, Ca 152.4-281.3%, Mg 28.3-79.4%, Fe 32.4-49.2ppm, Mn 26.6-41.1ppm...

Replant disease is one of the main growth limiting factors, interfering with plant growth and yield of stone fruit trees such as peach trees. The ecological feedback mechanisms by replanted soil microbes regulating peach growth and soil structure are rarely known. In our study, rhizosphere soils collected from 18-year-old peach trees were used to p...

Mycorrhizal hyphae possess the ability of absorbing water directly from the surrounding matrix to root, whereas it is not clear whether water flow redistributes from hyphae to surrounding matrix. In this study, potted trifoliate orange (Poncirus trifoliata) seedlings were inoculated with an arbuscular mycorrhizal (AM) fungus Funneliformis mosseae a...

The experiment was conducted to analyze the status of root colonization by arbuscular mycorrhizal (AM) fun