About
45
Publications
11,878
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
1,040
Citations
Publications
Publications (45)
Autores: García,T.; Garateix, A.; Palmero,A.; Valdés, A.; Valdés, O.; Aneiros, A. RESUMEN En la búsqueda de nuevas fuentes de productos naturales con actividades útiles para el hombre, las algas ocupan un lugar destacado por su utilización en la industria y la biomedicina. Se presentan los resultados de la evaluación de dos algas empleando técnicas...
Sea anemones produce proteinaceous toxins for predation and defense, including peptide toxins that act on a large variety of ion channels of pharmacological and biomedical interest. Phymanthus crucifer is commonly found in the Caribbean Sea; however, the chemical structure and biological activity of its toxins remain unknown, with the exception of...
Context: The extract from the marine plant Thalassia testudinum BM-21, standardized to thalassiolin B content (5.8 ± 0.3%), possesses antioxidant, anti-inflammatory and neuroprotective effects on acrylamide-induced neurotoxicity in mice and global ischemia in Mongolian gerbils.
Aims: To determine whether or not BM-21 possesses neuroprotective effec...
The aqueous ethanolic extract of the marine plant Thalassia testudinum, named BM-21, has powerful antioxidant and antilipoperoxidative activities. The extract also has anti-inflammatory, antinociceptive and neuroprotective effect against acrylamide-induced neurotoxicity. Excessive generation of free radicals and decreased levels of the antioxidant...
The Journal of Pharmacy & Pharmacognosy Research (JPPRes) is an international, specialized and peer-reviewed open access journal, which publishes studies in the pharmaceutical and herbal fields concerned with the physical, botanical, chemical, biological, toxicological properties and clinical applications of molecular entities, active pharmaceutica...
Context: Acrylamide (ACR) neurotoxicity is associated with the enhancement of lipid peroxidation and the reduction of the antioxidative capacity distal axon and nerve terminal regions. The aqueous ethanolic extract of the marine plant Thalassia testudinum, named BM-21, have shown antioxidant, anti-inflammatory and analgesic properties.
Aims: To de...
Sea anemones produce ion channels peptide toxins of pharmacological and biomedical interest. However, peptides acting on ligand-gated ion channels, including acid-sensing ion channel (ASIC) toxins, remain poorly explored. PhcrTx1 is the first compound characterized from the sea anemone Phymanthus crucifer, and it constitutes a novel ASIC inhibitor....
Sea anemones produce ion channels peptide toxins of pharmacological and biomedical interest. However, peptides acting on ligand-gated ion channels, including acid-sensing ion channel (ASIC) toxins, remain poorly explored. PhcrTx1 is the first compound characterized from the sea anemone Phymanthus crucifer, and it constitutes a novel ASIC inhibitor....
Sea anemones are sources of biologically active proteins and peptides. However, up to date few peptidomic studies of these organisms are known; therefore most species and their peptide diversity remain unexplored. Contrasting to previous venom peptidomic works on sea anemones and other venomous animals, in the present study we combined pH gradient...
Sea anemones are known to contain a wide diversity of biologically active peptides, mostly unexplored according to recent peptidomic and transcriptomic studies. In the present work, the neurotoxic fractions from the exudates of Stichodactyla helianthus and Bunodosoma granulifera were analyzed by reversed-phase chromatography and mass spectrometry....
Acid-sensing ion channels (ASICs) have a significant role in the sensation of pain and constitute an important target for the search of new antinociceptive drugs. In this work we studied the antinociceptive properties of the BM-21 extract, obtained from the sea grass Thalassia testudinum, in chemical and thermal models of nociception in mice. The a...
Because of their prominent role in electro-excitability, voltage-gated sodium (NaV) channels have become the foremost important target of animal toxins. These toxins have developed the ability to discriminate between closely related NaV subtypes, making them powerful tools to study NaV channel function and structure. CgNa is a 47-amino acid residue...
Sea anemone toxins bind to site 3 of the sodium channels, which is partially formed by the extracellular linker connecting S3 and S4 segments of domain IV, slowing down the inactivation process. In this work we have characterized the actions of BcIII, a sea anemone polypeptide toxin isolated from Bunodosoma caissarum, on neuronal sodium currents us...
Sticholysins I and II (St I/II) are cytolysins purified from the sea anemone Stichodactyla helianthus. In this study, we show their pharmacological action on guinea-pig and snail models in native and pH-denatured conditions in order to correlate the pharmacological findings with the pore-forming activity of both isoforms. In guinea-pig erythrocytes...
CgNa (Condylactis gigantea neurotoxin) is a 47-amino-acid- residue toxin from the giant Caribbean sea anemone Condylactis gigantea. The structure of CgNa, which was solved by 1H-NMR spectroscopy, is somewhat atypical and displays significant homology with both type I and II anemone toxins. CgNa also displays a considerable number of exceptions to t...
We have characterized the actions of ApC, a sea anemone polypeptide toxin isolated from Anthopleura elegantissima, on neuronal sodium currents (I(Na)) using current and voltage-clamp techniques. Neurons of the dorsal root ganglia of Wistar rats (P5-9) in primary culture were used for this study. These cells express tetrodotoxin-sensitive (TTX-S) an...
A new peptide toxin exhibiting a molecular weight of 5043Da (av.) and comprising 47 amino acid residues was isolated from the sea anemone Condylactis gigantea. Purification of the peptide was achieved by a multistep chromatographic procedure monitoring its strong paralytic activity on crustacea (LD(50) approx. 1microg/kg). Complete sequence analysi...
The Cuban marine flora and fauna is characterized by its richness in species that represents an extraordinary natural source of unexplored biomolecular diversity. Many of those biomolecules could represent important tools for industrial applications. For this reason, in our Centre the main aim was focused on the isolation, purification and characte...
Marine organisms represent a valuable source of new compounds. The biodiversity of the marine environment and the associated chemical diversity constitute a practically unlimited resource of new active substances in the field of the development of bioactive products. In this paper, the molecular diversity of different marine peptides is described a...
Toxin research in Cuba has made some important contributions to the field in recent decades. Most of the work carried out on marine toxins has been devoted to the isolation, purification, and characterization of polypeptide substances. The purification, molecular, and functional characterization as well as the pharmacological properties of these to...
We have characterized the effects of BgII and BgIII, two sea anemone peptides with almost identical sequences (they only differ by a single amino acid), on neuronal sodium currents using the whole-cell patch-clamp technique. Neurons of dorsal root ganglia of Wistar rats (P5-9) in primary culture (Leibovitz's L15 medium; 37 degrees C, 95% air/5% CO2...
We studied the effects of BgK toxin on outward K+ currents in isolated neurons of the snail Helix aspersa, using the whole cell patch clamp technique. BgK partially and reversibly blocked K+ currents in the 1 pM to 100 nM concentration range (n=53). The dose–response curve for BgK current inhibition had a maximum blocking effect at 100 nM. Our resu...
RESUMEN Las algas constituyen una fuente importante de compuestos de interés biomédico. En este estudio se presentan los resultados del pesquisaje realizado a extractos de 7 especies de algas en modelos de conducta exploratoria y convulsiones inducidas por choque eléctrico en ratones machos Balb-c y OF1 respectivamente, de 18-22 g de peso corporal;...
The effects were studied of a toxin (Bainh) isolated from the secretion of the Caribbean sea anemone Bunodosoma granulifera on electrical and mechanical activities of rat ventricular muscle. The effects on the ionic currents of single rat and dog ventricular cardiomyocytes were studied using the whole-cell recording patch-clamp technique. In the co...
In the search for new glutamate antagonists it seems promising to characterize the effects of venom from invertebrates that prey mainly on crustaceans. In this work, the exudate of the sea anemone Phyllactis flosculifera was used as a source of this type of compound. The action of chromatographic fraction D from P. flosculifera was tested upon micr...
En el presente trabajo se realizó el aislamiento y purificación de una toxina anticolinérgica de alto peso molecular presente en la anemona marina Condylactis gigantea. Los extractos proteicos fueron fraccionados utilizando Sephadex G-50 como primera etapa de purificación e intercambio iónico en DEAE-Celulosa 52 en sistema semi-batch como segunda e...
La purificación de una toxina de elevado peso molecular a partir de la anémona marina Condylactis gigantea se logró mediante la combinación de Ia filtración en gel y cromatografía de intercambio iónico. Los extractos de partida fueron preparados a partir de la pared de la columna corporal del animal. La filtración en gel de Sephadex G-50 permitió I...
En el presente trabajo se realizó el aislamiento y purificación de una toxina anticolinérgica de alto peso molecular presente en la anemona marina Condylactis gigantea. Los extractos proteicos fueron fraccionados utilizando Sephadex G-50 como primera etapa de purificación e intercambio iónico en DEAE-Celulosa 52 en sistema semi-batch como segunda e...
1. The effects of a high molecular weight toxin isolated from the sea anemone Condylactis gigantea (Condytoxina 2) on the cholinergic responses were studied in two different preparations: identified cells of a land snail and enzymatically dissociated mice sensory neurons. These neurons were studied using intracellular recording and concentration cl...
A description of the activation phase of the land snail Zachrysia guanensis delayed potassium current (IK) is presented. It was found that IK activation kinetics may be congruent with the Hodgkin-Huxley scheme if one assumes that the proportion of n particles at the beginning of the pulse is not zero. In this case IK activation may be treated as ca...
1. Quinidine was applied to isolated, voltage-clamped, internally perfused neurons of Zachrysia guanensis.2. The apparent inhibitory constant for the block of potassium delayed current was about 25 μM.3. Quinidine induces an increase in the inactivation rate, probably independent of the effects upon current amplitude.4. These results differ of thos...
A computer program based on the Gauss-Newton method was developed for the Hodgkin-Huxley estimation of kinetic parameters of membrane currents recorded in voltage-clamp experiments. Fast potassium current of land snail neurons was estimated, and found to be in agreement with literature reports.
1.1. The experiments were performed on enzymatically isolated neurons from the Cuban land snail Zachrysia guanensis.2.2. The method of intracellular perfusion and voltage clamp was used to evaluate the effect of the Na+ and K+ currents of these neurons of Condylactis gigantea phospholipase A externally and internally applied.3.3. A preferential act...
1.1. The nicotinic anticholinergic effects of Physalia-toxin P1, a purified polypeptide (MW 220 Kdalton) of Physalia physalis tentacles extracts, was tested on two identified neurons of the land snail Zachrysia quanensis and on frog (Rana pipiens) neuromuscular junction.2.2. P1 reversibly blocks in a dose-dependent manner the nicotinic cholinergic...
The effects of a high molecular weight toxin from Physalia physalis (P3) were investigated on glutamate evoked potentials in snail (Zachrysia guanesis) neurons and in crayfish (Cambarus clarkii) neuromuscular junction. The glutamate evoked potentials of snail neurons were reversibly blocked by P3 in a dose-dependent manner (2-200 microM). A reversi...
Summary Voltage dependent sodium channels represent an important target for different neurotoxins and there have been identified different binding sites according to these interactions. The so called site 3 toxins comprise a diverse group of peptides obtained from sea anemones and α-scorpions that bind to voltage gated sodium channels slowing down...
We have characterized the effects of BgII and BgK, two sea anemone peptides obtained from the sea anemone Bunodosoma granulfíera on neuronal currents using whole-cell patch clamp technique. Neurons of dorsal root ganglia of Wistar rats (P5-9) in primary culture (L-15 medium, 37 ºC, 95% air, 5% CO2) were used for this study (n =112). BgII produced a...
