Anoja Priyadarshani Attanayake

Anoja Priyadarshani Attanayake
University of Ruhuna · Department of Biochemistry

BSc (Chemistry), PhD (Biochemistry)
Professor in Biochemistry, Faculty of Medicine, University of Ruhuna

About

92
Publications
17,860
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
311
Citations
Citations since 2016
77 Research Items
304 Citations
2016201720182019202020212022020406080100
2016201720182019202020212022020406080100
2016201720182019202020212022020406080100
2016201720182019202020212022020406080100

Publications

Publications (92)
Article
The search for therapeutic agents that improve kidney function against doxorubicin-induced renal toxicity is important. Herein, the potential nephroprotective activity by Asparagus falcatus L. (AF, Asparagaceae) leaf extracts against doxorubicin-induced renal toxicity (5 mg/kg, ip) in Wistar rats (n = 6/group) after oral administration of hexane (5...
Article
Introduction : Despite the wisdom of using medicinal flora as antidiabetic therapies by practitioners of Ayurveda and traditional medicine, the process of documenting this knowledge is untapped to date. The objective of the present survey was to record the knowledge and the utility of the medicinal plants in antidiabetic remedies that have been use...
Article
A new dimeric carbazole alkaloid, 3,3′ ,5,5′,8-pentamethyl-3,3′ -bis(4-methylpent-3-en-1-yl)-3,3′ ,11,11′ -tetrahy-dro-10,10′ -bipyrano[3,2-a]carbazole, was isolated from the hexane extract of leaves of Murraya koenigii (L.) Sprengel. (Family: Rutaceae). The structure was elucidated based on 13C and 1H NMR, High- Resolution Mass Spectrometry (HRMS)...
Article
Full-text available
Aim: Medicinal flora is rich with bioactive compounds that exhibit promising antioxidant activities thereby protecting against free radical pathologies leading to health-promoting effects. The use of potent antioxidants is considered a substantial approach to prevent or diminish the risk of many dative stress-related diseases. The present review in...
Article
Full-text available
Coccinia grandis (L.) Voigt (family: Cucurbitaceae) is a popular leafy vegetable in Sri Lankan diet. C. grandis is high in phenolics and flavonoids. The present work attempted to determine a suitable extraction technique, and further optimise it to obtain phenolic-and flavonoid-rich extract from C. grandis leaves, with an aim at developing a nutrac...
Conference Paper
Full-text available
Garcinol is naturally rich in many plants of the Garcinia genus, including Garcinia quaesita Pierre, and it is a derivative of polyisoprenylated benzophenone. The in vivo antidiabetic potential of garcinol has been reported in different previous studies. However, in vitro antidiabetic potential of the compound remain limited details. In vitro tests...
Conference Paper
Full-text available
An experimental animal model of type 2 diabetes mellitus (T2DM) facilitates the studying of antidiabetic and antioxidative effects of novel drugs. Feeding high-fat diet (HFD) followed by a low-dose streptozotocin (STZ) injection is used to induce diabetes and oxidative changes in the experimental animal model of diabetes.
Article
Full-text available
This study focused on qualitative and quantitative analysis of phytochemicals and the study of antioxidant and acetylcholinesterase (AChE) inhibitory activities of fruit pulps of three abundant banana varieties grown in Sri Lanka. Aqueous, ethanol, ethyl acetate and hexane extracts of nearly three-month-old fruit pulps of Musa accuminata AAA, Musa...
Article
Full-text available
Polyherbal preparations have gained much attention as a potential source for discovering new drug therapeutics for the treatment of diabetes mellitus. The present investigation aims to determine in vitro and in vivo antidiabetic activity and the antioxidant potential of hexane, ethyl acetate and methanol extracts of a polyherbal mixture prepared fr...
Article
Full-text available
The scarcity of novel drugs has prompted scientific research to re-evaluate natural products as drug lead compounds with high chemical and biological potential. Among the many phytochemicals, alkaloids are an important group of natural products consisting of an extensive list of bioactivities such as antitumor, anti-inflammatory, antiviral, antihyp...
Article
Full-text available
Background Herbal remedies are used to manage type 2 diabetes mellitus (type 2 DM) as the sole treatment or as a complementary therapy. Limitations of herbal remedies, such as poor stability and limited absorption, impede their development as therapeutic agents, which could be overcome by nanoformulations. Objectives This review attempts to summar...
Article
Absteact Ethnopharmacological relevance Ambrette (Abelmoschus moschatus Medik., Family: Malvaceae) is a common Ayurvedic herbal medicine used in the treatment of kidney-related diseases, in the forms of tea, medicated oil, medicated wine, etc., however, its nephroprotective mechanisms remain unexploited. Aim of the study To investigate the mechan...
Conference Paper
Full-text available
Low solubility and degradation under unfavorable conditions limit the bioactivities of herbal extracts. Previous literature supports that herbal extract encapsulated nanoformulations exhibit remarkable inhibitory potential on carbohydrate hydrolysing enzymes. This study aimed to characterize the aqueous extract of Catharanthus roseus L. (Family: Ap...
Conference Paper
Full-text available
Antidiabetic mechanisms of an Ayurvedic polyherbal mixture composed of dried fruit rinds of Garcinia queasita Pierre, leaves of Murraya koenigii L. Spreng., seeds of Piper nigrum L. and cloves of Allium sativum L. are scientifically untapped. In the present evaluation, the effect of the hexane extract of the mixture on the enzyme activities of the...
Article
The development of a stable disease model with an adequate biochemical profile is crucial for the preclinical investigation of new antidiabetic agents. This study aimed at optimization and characterization of high fat diet (HFD) fed streptozotocin (STZ) induced type 2 diabetes mellitus (type 2 DM) Wistar rat model. Wistar rats fed with HFD for four...
Article
Development of novel wound dressing materials having the ability to prevent bacterial infections and capable of accelerating the tissue regeneration process is utmost important, since the wounds in patients can cause severe health issues. In the present work, we synthesized novel N-succinyl chitosan nanoparticles (N-SuC NPs) films and tested their...
Conference Paper
Full-text available
Introduction and objectives: Development of phytotherapeutics using nanoencapsulation is a timely approach to enhance the antidiabetic activity. Aegle marmelos L. (Bael, Family: Rutaceae) has been used in complementary medicine with proven antidiabetic activity. A previous study confirmed that the 1 mg/mL concentration of A. marmelos showed the opt...
Article
IntroductionDysglycemia includes prediabetes and diabetes. We aimed to study the prevalence of dysglycemia, and its associations with age and body mass index (BMI) among community dwelling adults in Sri Lanka.Methods The prevalence of dysglycemic state (FPG > 100 mg/dL) and its associations with age and BMI in males and females were estimated. The...
Article
Full-text available
A folksy polyherbal remedy used in the traditional medicine system made of seeds of Piper nigrum L., leaves of Murraya koenigii L. Sprengel., cloves of Allium sativum L. and dried fruit rinds of Garcinia quaesita Pierre is considered as an efficient treatment for the dyslipidaemia associated with diabetes mellitus by Ayurvedic medical practitioners...
Article
Full-text available
The nephroprotective effect of standardized aqueous root extract of Vetiveria ziza�nioides (L.) Nash (Family: Poaceae) was investigated in doxorubicin-induced (20 mg/ kg, ip) experimental nephrotoxicity model of Wistar rats. The freeze-dried aqueous refluxed (4 hr) root extract of V. zizanioides (25, 50; equivalent human therapeutic dose and 100...
Conference Paper
Full-text available
Background: Development of alginate nanoparticles loaded with extract of Coccinia grandis L. (Ivy Gourd, Family: Cucurbitaceae) is a prospective approach to enhance the antidiabetic activity of the aqueous leaf extract of C. grandis. Objective: To assess in vitro antidiabetic activity of aqueous leaf extract of C. grandis encapsulated alginate nan...
Conference Paper
Full-text available
Glucose-lowering activity of Coccinia grandis (L.) Voigt leaf extract encapsulated nanoliposomes in Wistar rats induced with diabetes mellitus Background: Glucose-lowering activity of polyphenol rich aqueous extracts of Coccinia grandis (L.) Voigt (Family: Cucurbitaceae) has been scientifically proven. However, it was reported that the C. grandis e...
Article
Full-text available
There is an increasing trend of investigating natural bioactive compounds targeting pancreatic β-cells for the prevention/treatment of diabetes mellitus (DM). With the exploration of multiple mechanisms by which β-cells involve in the pathogenesis of DM, herbal medicines are gaining attention due to their multitasking ability as evidenced by tradit...
Article
Full-text available
Background Insulin resistance (IR) has been considered as a therapeutic target in the management of type 2 diabetes mellitus (T2DM). Readily available, simple and low cost measures to identify individuals with IR is of utmost importance for clinicians to plan optimal management strategies. Research on the associations between surrogate markers of I...
Conference Paper
Full-text available
Background: Gmelina arborea Roxb. is a medicinal plant used in complementary medicine with antidiabetic properties. G. arborea aqueous extract (GAE) has been reported to exert α-amylase inhibitory activity. However, low bioavailability of polyphenols and flavonoids, and lack of appropriate delivery systems delimit use of GAE as a commercially viabl...
Conference Paper
Full-text available
Background: Nanoencapsulated herbal extracts have gained significant interest in the management of diabetes mellitus. Determination of polyphenol, flavonoid contents and alpha amylase inhibition activity is a substantial approach to screen herbal extracts and to develop commercially viable nanoencapsulated herbal formulations. Objectives: To deter...
Conference Paper
Full-text available
Decoctions prepared from the bark of Gmelina arborea have been employed in Sri Lankan traditional medicine as a remedy against diabetes mellitus. Encapsulation of bioactive secondary metabolites of G. arborea could enhance their therapeutic potential and provide controlled release. Amongst the different matrices available as options to encapsulate...
Conference Paper
Full-text available
The homeostatic model assessment of insulin resistance (HOMA-IR) and-cell functions (HOMA-recognized as important indices in evaluating the preclinical efficacy of novel antidiabetic agents. The present investigation was aimed to evaluate the effect of hexane extract of a Ayurvedic polyherbal mixture made from Allium sativum L. (cloves), Murraya ko...
Conference Paper
Full-text available
Nanoencapsulation is an innovative approach to overcome the limitations of conventional herbal extracts that are used as therapeutic agents. The optimization of conditions in the preparation of nanoparticles is critical in the development of nanonutraceutical formulations from edible plant extracts. The objective of the present study was to optimiz...
Article
Full-text available
The use of medicinal plant extracts and their isolated bioactive compounds for the management of diabetes mellitus has been tremendously increased in recent decades. The present study aimed at providing in-depth information on medicinal flora that has been widely used in the Sri Lankan traditional healthcare system for the management of diabetes me...
Article
Full-text available
Proteinuria is an important prognostic marker in the diagnosis and management of kidney diseases. Sulfosalicylic acid method (SSA) is a simple, low cost, qualitative test, widely used to assess proteinuria. The aim of this study was to optimize SSA test as a quantitative screening tool to assess proteinuria at lower excretory levels which would fac...
Conference Paper
Full-text available
Medicinal plants are often rich in polyphenols and flavonoids, some of which are reported to possess anti-diabetic properties. In this study, Spondias pinnata (L.f) Kurz and Gmelina arborea Roxb were selected based on the ethnomedical usage in the management of diabetes mellitus. Crude extracts were screened for the presence of high-amylase using i...
Article
Full-text available
The correct volume of sample and time of storing prior to the analysis are important considerations in the estimation of plasma glucose concentration of patients. The present study was to determine the effect of sample volume variation and time delay in the analysis of plasma glucose results in healthy adults. A total of 30 individuals aged between...
Article
Ethnopharmacological relevance Traditional polyherbal preparations have been utilized in Sri Lanka since ancient times and have gained a wide acceptance throughout the country. Although an extensive body of evidence supports the use of traditional herbal mixtures in the treatment of diabetes mellitus, only a few polyherbal mixtures have been subjec...
Article
Background Several lines of preclinical studies have shown promising antidiabetic effects of the aqueous leaves extract of Coccinia grandis (Linn.) Voigt (Cucurbitaceae) in vivo and in vitro. Purpose : The present study was conducted to evaluate the efficacy and safety of a newly developed herbal formulation of C. grandis in newly diagnosed patien...
Article
Ethnopharmacological relevance: Traditional polyherbal preparations have been utilized in Sri Lanka since ancient times and have gained a wide acceptance throughout the country. Although an extensive body of evidence supports the use of traditional herbal mixtures in the treatment of diabetes mellitus, only a few polyherbal mixtures have been su...
Conference Paper
Full-text available
Introduction: Polyphenols and flavonoids have an array of health-promoting benefits; they are of current interest due to their vital biological and pharmacological properties including the possible role in preventing cardiovascular diseases and cancer. The geographical location is an important determinant in the collection of plants for preparation...
Conference Paper
Full-text available
A homemade Ayurvedic remedy made of cloves of Allium sativum, leaves of Murraya koenigii, seeds of Piper nigrum and dried fruit rinds of Garcinia quaesita is considered as an antidiabetic polyherbal mixture. This polyherbal mixture has been used for the treatment of diabetes mellitus and dyslipidaemia in Sri Lankan traditional medicine. The in-vitr...
Article
Full-text available
Although science education, including biochemistry and molecular biology education, starts before students commence university, for many students, undergraduate programs are their first real introduction to biochemistry and molecular biology. Students often report that biochemistry and molecular biology are relatively difficult topics hence the use...
Article
Full-text available
The dried fruit of Garcinia quaesita Pierre (Clusiaceae), is a well-known endemic spice in Sri Lanka and has been documented in traditional medicine as an antidiabetic agent. The antihyperglycemic effect of cold water, hot water, water : acetone fruit extracts of G. quaesita and its isolated compound, garcinol in streptozotocin-induced diabetic rat...
Article
Full-text available
Chronic kidney disease remains as one of the serious health issues in South Asia. The paucity of effective pharmacotherapy targeting the management of chronic kidney disease has led to a search for alternative pharmacologic therapies. The traditional knowledge of medicinal plants plays a key role in the discovery of novel nephroprotective agents. T...
Article
Full-text available
Nephrotoxicity is a major limitation of adriamycin (ADR) chemotherapy. We hypothesized that administration of standardized aqueous bark extract of Gmelina arborea Roxb. (GA) (Family; Verbenaceae), a traditional therapeutic agent, may reduce the nephrotoxicity caused by ADR in Wistar rats. The dose-dependent nephroprotective activity of the standard...
Article
Full-text available
A polyherbal drug composed of leaves of Murraya koenigii L. Spreng, cloves of Allium sativum L., fruits of Garcinia quaesita Pierre, and seeds of Piper nigrum L. is a popular drug which has been used by indigenous practitioners in Sri Lanka for the treatment of diabetes mellitus and dyslipidemia. The acute toxicity assessment was conducted, followi...
Article
Full-text available
Abelmoschus moschatus Medik. (family: Malvaceae) has a long history of being used as a folk medicine in Sri Lanka. Despite the therapeutic use of this plant in traditional medicine, leaves of A. moschatus have not been subjected to scientific evaluation of toxicity/adverse effects in vivo. Thus, the present study was aimed to assess the acute and 2...
Article
Ethnopharmacological relevance Abelmoschus moschatus Medik. (family: Malvaceae), Asparagus falcatus (family: Asparagaceae) and Barleria prionitis Linn. (family: Acanthaceae) have been used in the treatment of kidney diseases in Sri Lankan traditional medicine. Besides the traditional use, scientific scrutinisation of safe therapeutic use of these m...
Article
Full-text available
Diagnostic tools used in detecting individuals with diabetes mellitus (DM) include fasting plasma glucose (FPG), glycated hemoglobin (HbA1C), and oral glucose tolerance test (OGTT). The present study was aimed to determine the demographic associations of diabetes status by both tests (FPG and HbA1C) in Galle district, Sri Lanka. 147 adults (30–60 y...
Article
Full-text available
Diabetes mellitus has become a global epidemic over the past few decades and is one among the major causes of death worldwide. Although various modern medicines are in the market, herbal medicines have usually maintained worldwide popularity in achieving the primary health care needs of the diabetic population because of the multiple adverse effect...
Article
Full-text available
The antioxidant activity of garcinol has been identified as the basis for various bioactivities in it, and the genus Garcinia is the main source for the garcinol. G. quaesita, which is endemic to Sri Lanka, is a representative member of genus Garcinia, and the dried fruit rind of G. quaesita is used practically in all curry preparations to impart a...
Conference Paper
Full-text available
Introduction: Proteinuria is an important prognostic marker in diagnosis and management of Chronic Kidney Disease (CKD). Previously, 25% sulfosalicylic acid (SSA) method has been optimized as a cost-effective quantitative method to evaluate proteinuria in the lower range (0-50 mg/dL). This study was conducted to assess the clinical applicability by...
Article
Full-text available
The present study aimed to determine the total polyphenol content and total antioxidant activities of ten selected seasonal fruit extracts grown in Sri Lanka. The aqueous refluxed fruit extracts (4h) were used at the initial concentration of 0.05 g/mL. The total polyphenol content was determined according to the Folin-Ciocalteu method. The total an...
Conference Paper
Full-text available
Background: Morbidity and mortality due to diabetes mellitus have been considered as a major public health problem in Sri Lanka over the last two decades. Many oral antidaibetic agents, such as meglitinides, biguanides etc. have recorded considerable side effects. Thus, there is an increasing necessity of natural products with high antidiabetic pot...
Article
Full-text available
Animals are used as experimental models to reproduce human diseases. To date, various animal models have been successfully developed by numerous methods to simulate human diseases including chronic kidney disease (CKD). Such models have played a central role in developing dialysis, transplantation experiments and more importantly in the discovery o...
Article
Full-text available
Animals are used as experimental models to reproduce human diseases. To date, various animal models have been successfully developed by numerous methods to simulate human diseases including chronic kidney disease (CKD). Such models have played a central role in developing dialysis, transplantation experiments and more importantly in the discovery o...
Article
Full-text available
The objectives of the study were to determine the acetylcholinesterase (AChE) inhibitory activity and antioxidant activity in the selected Sri Lankan medicinal plants. AChE inhibitory activity of the selected medicinal plant extracts was determined using the Ellman’s method. The antioxidant activities were determined by four in vitro methods namely...
Article
Dietary polyphenols, mainly phenolic acids and flavonoids, are the primary sources of antioxidants that can be generally recommended for human consumption. Polyphenols are derived from plant based food including fruits, vegetables, spices, herbs etc. In the present era, there has been a great interest towards the health benefits of dietary polyphen...
Article
Animals are used as experimental models to reproduce human diseases. To date, various animal models have been successfully developed by numerous methods to simulate human diseases including chronic kidney disease (CKD). Such models have played a central role in developing dialysis, transplantation experiments and more importantly in the discovery o...
Article
Full-text available
The objective of the present study was to investigate the effect of refluxed aqueous extracts of Gmelina arborea , Spondias pinnata , and Coccinia grandis on atherogenicity and oxidative stress in rats with chemically induced type 1 diabetes mellitus. Alloxan monohydrate (150 mg/kg, intraperitoneal) was used to induce diabetes to Wistar rats. There...
Article
Full-text available
Background: Effective β-cell regeneration is a recognized therapeutic strategy in the treatment of type 1 diabetes mellitus. Regeneration of β-cells could be achieved via exogenous natural sources as medicinal plant extracts. Medicinal plants selected for the investigation were Spondias pinnata (Linn. f.) Kurz, Coccinia grandis (L.) Voigt and Gmel...
Article
Full-text available
Gmelina arborea Roxb. (common name: Et-demata, Family: Verbenaceae) has been used traditionally in Sri Lanka as a remedy against diabetes mellitus. The objective of the present study was to evaluate antidiabetic mechanisms of the aqueous bark extract of G. arborea in streptozotocin induced (STZ) diabetic male Wistar rats. Aqueous bark extract of G....
Article
Full-text available
A wide array of Sri Lankan medicinal plants has been used for the treatment of many chronic diseases such as diabetes mellitus, liver diseases and arthritis in Ayurvedic medicine. Antioxidants are reported to play central roles in reducing or preventing free radical damage in the above oxidative stress related diseases. The present study was conduc...