Anne Ponstein

Anne Ponstein
University of Applied Sciences Leiden | UAS

Bachelor of Arts/ Master of science
Setting up my own practice as researcher/ art therapist in the psycho-oncological field. Looking for collaborations!

About

50
Publications
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2,135
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Publications

Publications (50)
Article
Full-text available
Introduction The aim of this study was to develop a prototype of an anthroposophic complex intervention (CI) for oncological patients in primary care. Methods Standardized methods for the development of CIs were used. Qualitative data were collected among professionals (n = 44) working in 3 Dutch anthroposophic primary care centers. The following...
Article
Full-text available
Anxiety is a major problem for many individuals, causing impairment in daily life. Art therapy is often deployed and although positive results are communicated in clinical practice, its effectiveness and working mechanisms have hardly been studied. Therefore, it is important to systematically describe the intervention process and to detect the work...
Article
Full-text available
Objectives: Art therapy as a treatment option for anxiety is regularly employed in clinical practice, but scientific evidence for its effectiveness is lacking, since this intervention has hardly been studied. The aim was to study the effectiveness of art therapy (AT) on anxiety in adult women. The specific type of AT studied was anthroposophic art...
Article
Full-text available
Background Anxiety disorders are one of the most diagnosed mental health disorders. Common treatment consists of cognitive behavioral therapy and pharmacotherapy. In clinical practice, also art therapy is additionally provided to patients with anxiety (disorders), among others because treatment as usual is not sufficiently effective for a large gro...
Data
Background characteristics of the included studies. (PDF)
Data
Full list of search terms and databases. (PDF)
Data
Excluded studies with reasons for exclusion. (PDF)
Article
De maatschappij vraagt in toenemende mate om gegevens die de werkzaamheid en het effect van vaktherapie aantonen. Tevens is er – vooral vanuit de beroepsgroepen – een vraag naar het expliciteren van de werkingsmechanismen van de vaktherapieën. Een bijdrage aan het beantwoorden van beide vragen kan op basis van casusbeschrijvingen gegeven worden, mi...
Article
Objectives: The aim of the study was to develop a whole medical system healthcare program as an alternative for group-oriented guidelines and as a basis for future studies on safety and (cost) effects. Mild to moderately severe depressive disorders were chosen as an illustrative example. Design: Literature surveys, monodisciplinary expert interv...
Article
Anthroposophic art therapists (AATs) report individual cases in narratives of poor scientific quality. Good quality case reports are an important factor in the development of evidence-based practice. A guideline for scientific case reports could contribute to this. However, the recently published guideline for medical case reports (the CARE Guideli...
Article
Full-text available
depression, patients frequently have physical complaints and body-image disturbances.1 Somatic symptoms are now re-garded as “common presenting features throughout the world.”2These are also expressions of psychosomatic processes.The therapeutic strategy in body psychotherapy (BPT) is experiential and relational.3 According to Heller, it includes “...
Article
In a search for novel plant-derived antimicrobial proteins, we screened extracts from salicylic acid (SA)-treated lettuce and sunflower leaves. These extracts displayed very potent antimicrobial activity against a set of phytopathogens. Characterisation of these extracts revealed that in both extracts, proteins of approximately 60 kDa were responsi...
Article
A simple strategy to identify and isolate new promoters suitable for driving the expression of selectable marker genes is described. By employing a Brassica napus hypocotyl transformation protocol and a promoterless gus::nptII tagging construct, a series of 20 kanamycin-resistant tagged lines was produced. Most of the regenerated plants showed hard...
Article
TheAspergillus niger gene encoding phytase(phyA) was expressed in canola (Brassicanapus). Phytase expression is controlled by the seed-specificcruciferin (CruA) promoter. Secretion of the enzyme was aimed for byincorporating the cruciferin signal peptide in the expression construct.Transgenic canola lines were generated by Agrobacteriummediated tra...
Article
Full-text available
As a first step toward the exploitation of the disaccharide trehalose as a stress-protective and preservative agent in plants, we engineered trehalose biosynthesis in tobacco (Nicotiana tabacum) and potato (Solanum tuberosum) by introducing the otsA and otsB genes from Escherichia coli, which encode trehalose-6-phosphate synthase and trehalose-6-ph...
Article
A novel chitinase gene of tobacco was isolated and characterized by DNA sequence analysis of a genomic clone and a cDNA clone. Comparative sequence analysis of both clones showed an identity of 94%. The proteins encoded by these sequences do not correspond to any of the previously characterized plant chitinases of classes I-IV and are designated as...
Article
Full-text available
A novel pathogen- and wound-inducible antifungal protein of 20 kD was purified from tobacco (Nicotiana tabacum) Samsun NN leaves inoculated with tobacco mosaic virus (TMV). The protein, designated CBP20, was purified by chitin-affinity chromatography and gel filtration. In vitro assays demonstrated that CBP20 exhibits antifungal activity toward Tri...
Article
A novel pathogen- and wound-inducible antifungal protein of 20 kD was purified from tobacco (Nicotiana tabacum) Samsun NN leaves inoculated with tobacco mosaic virus (TMV). The protein, designated CBP20, was purified by chitin-affinity chromatography and gel filtration. In vitro assays demonstrated that CBP20 exhibits antifungal activity toward Tri...
Chapter
An Escherichia coli clone containing the ftf gene of Streptococcus mutans encoding an inulosucrase was studied in in vitro experiments. The enzyme transferred fructose units donated by sucrose to water as well as to sucrose (and fructo-oligosaccharides). The apparent Km of the “self-transfer” reaction was 18 ± 2 mM. In the presence of inu...
Article
Full-text available
Different isoforms of chitinases and [beta]-1,3-glucanases of tobacco (Nicotiana tabacum cv Samsun NN) were tested for their antifungal activities. The class I, vacuolar chitinase and [beta]-1,3-glucanase isoforms were the most active against Fusarium solani germlings, resulting in lysis of the hyphal tips and in growth inhibition. In additon, we o...
Chapter
The phenomenon of induced resistance in plants is accompanied by the induction of the synthesis of a large number of proteins. Here we demonstrate that among these proteins the intracellular class I chitinases, ß-1,3-glucanases and AP24 exhibit antifungal activity in vitro. In contrast, the class II isoforms of these induced proteins show limited o...
Article
A soluble starch synthase preparation obtained from potato tubers (Solanum tuberosum L.) was used to identify the acceptor molecules of the enzyme in vitro. Maltose was the shortest effective primer molecule, and several straight and branched glucose polymers were also able to serve as primer. Osmotically permeabilized potato tissue slices were use...
Article
Full-text available
The genomic sequence of the potato gene for starch granule-bound starch synthase (GBSS; waxy protein) has been determined for the wild-type allele of a monoploid genotype from which an amylose-free (amf) mutant was derived, and for the mutant part of the amf allele. Comparison of the wild-type sequence with a cDNA sequence from the literature and a...
Article
The amylose-free (amf) potato clone 86.040 (2n = 1x = 12), in which the enzyme granule bound starch synthase (GBSS) is affected, has been used for the induction and isolation of revertants with loosely branched amylopectin. Screening of 5500 microtubers, which were induced on stem segments of 685 irradiated buds gave in 44 cases reverted sectors fr...
Article
Full-text available
Osmotically permeabilized potato (Solanum tuberosum L.) tuber slices were used to study the biosynthesis of starch under semi in vivo conditions. Criteria to distinguish the various enzymes involved in starch biosynthesis were developed based on the characteristics of the enzymes in in vitro experiments. Branching enzyme activity was inhibited at p...
Article
Thesis statement, foreword, and summary in Dutch. "Stellingen" ([2] p.) inserted. Thesis (doctoral)--Rijksuniversiteit Groningen, 1990. Includes bibliographical references (p. 106-119).
Article
Full-text available
Osmotically permeabilized potato (Solanum tuberosum L.) tuber slices were used to study the biosynthesis of starch under semi in vivo conditions. Criteria to distinguish the various enzymes involved in starch biosynthesis were developed based on the characteristics of the enzymes in in vitro experiments. Branching enzyme activity was inhibited at p...
Article
Starch branching enzyme was purified from potato (Solanum tuberosum L.) tubers as a single species of 79 kilodaltons and specific antibodies were prepared against both the native enzyme and against the gel-purified, denatured enzyme. The activity of potato branching enzyme could only be neutralized by antinative potato branching enzyme, whereas bot...
Article
Two important starch enzymes, granule-bound starch synthase and branching enzyme, were purified from potato tubers and characterized by immunological comparison with the corresponding enzymes of other plants. Granule-bound starch synthase was identified as a 60-kd protein homologous to the corresponding enzymes of maize and amaranth; the enzyme was...
Article
An amylose-free potato mutant was isolated after screening 12,000 minitubers. These minitubers had been induced on stem segments of adventitious shoots, which had been regenerated on leaf explants of a monoploid potato clone after Rntgen-irradiation. The mutant character is also expressed in subterranean tubers and in microspores. Starch granules f...
Article
Osmotically permeabilized potato (Solanum tuberosum L.) tuber slices wereusedtostudy thebiosynthesis ofstarch under semiinvivoconditions. Criteria todistinguish thevarious en- zymesinvolved instarch biosynthesis weredeveloped basedon thecharacteristics oftheenzymes ininvitro experiments. Branch- ingenzymeactivity wasinhibited atpH8.5orhigher, while...

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