Anne Elizabeth Peaston

University of Adelaide · School of Animal and Veterinary Sciences

Mast cell tumours (MCT) have been documented in numerous species and mutations within the KIT proto‐oncogene are implicated in the neoplastic biology of mast cells in humans, dogs, and cats. This study determined high KIT gene nucleotide and Kit amino acid sequence homology between several species known to suffer mast cell neoplasia and especially...

Mast cell tumors in nondomestic felids are rarely reported and their biological characteristics are not well described. A retrospective review of the pathology records of 52 zoo-housed cheetahs (Acinonyx jubatus) identified five cases of mast cell tumor, involving four closely related individuals. The age at initial presentation varied from 14 mo t...

In this study, three different diagnostic tests for parvovirus were compared with vaccination status and parvovirus genotype in suspected canine parvovirus cases. Faecal samples from vaccinated (N17) and unvaccinated or unknown vaccination status (N41) dogs that had clinical signs of parvovirus infection were tested using three different assays of...

Mast cell neoplasia clinical presentation and biological behaviour vary considerably across mammalian species, ranging from a solitary benign mass to an aggressive systemic malignancy. Mutations in the KIT Proto‐Oncogene Receptor Tyrosine Kinase (KIT) gene are common molecular abnormalities involved in mast cell tumorigenesis. KIT mutations often o...

DNA amplification by PCR detects KIT exon 11 internal tandem duplications in canine mast cell tumors (MCTs). Tissue-specific inhibitors often contaminate DNA extracted from formalin-fixed, paraffin-embedded (FFPE) canine MCTs, blocking PCR amplification and, consequently, preventing mutation detection. We used a commercial kit to extract DNA from F...

Osteosarcoma is the most common pediatric primary bone malignancy. The major cause of death in osteosarcoma is drug‐resistant pulmonary metastasis. Previous studies have shown that thioredoxin reductase 2 is a driver of metastasis in osteosarcoma and can be inhibited by auranofin. Moreover, studies have shown that auranofin significantly reduces pu...

In Australia, compulsory microchipping legislation requires that animals are microchipped before sale or prior to 3 months in the Australian Capital Territory, New South Wales, Queensland and Victoria, and by 6 months in Western Australia and Tasmania. Describing the implementation of microchipping in animals allows the data guardians to identify i...

Little is known about genetic causes of congenital methemoglobinemia in dogs. Here, we report a CYB5R3 mutation in a Pomeranian dog with congenital methemoglobinemia. A 6‐year‐old neutered female Pomeranian dog was investigated for cyanosis noticed during anesthesia for an orthopedic procedure. The history included lifelong mild exercise intoleranc...

The aim of the study was to develop a qPCR assay for diagnosis and monitoring of mycoplasma urinary tract infections (UTI) in a dogs. An English Cocker Spaniel dog with the history of urinary tract infection was physically examined and laboratory findings identified chronic renal insufficiency and urinary tract infection. Attempts to culture organi...

Objective: To measure the prevalence of internal tandem duplications (ITDs) in exon 11 of the proto-oncogene C-KIT in a sample of Australian cutaneous canine mast cell tumours (MCTs) drawn from general practice and to evaluate relationships between tumour mutation status and prognostic factors including signalment, tumour histological grade, tumou...

VetCompass Australia is veterinary medical records-based research coordinated with the global VetCompass endeavor to maximize its quality and effectiveness for Australian companion animals (cats, dogs, and horses). Bringing together all seven Australian veterinary schools, it is the first nationwide surveillance system collating clinical records on...

A common approach for developing diagnostic tests for influenza virus detection is the use of mouse or rabbit monoclonal and/or polyclonal antibodies against a target antigen of the virus. However, comparative mapping of the target antigen using antibodies from different animal sources has not been evaluated before. This is important because identi...

Detailed statistical analysis performed in this study. (DOCX)

Vaccination is becoming a more acceptable option in the effort to eradicate avian influenza viruses (AIV) from commercial poultry, especially in countries where AIV is endemic. The main concern surrounding this option has been the inability of the conventional serological tests to differentiate antibodies produced due to vaccination from antibodies...

Mammalian oocytes are arrested at prophase I of meiosis, and resume meiosis prior to ovulation. Coordination of meiotic arrest and resumption is partly dependent on the post-transcriptional regulation of maternal transcripts. Here, we report that, SPINDLIN1 (SPIN1), a maternal protein containing Tudor-like domains, interacts with a known mRNA-bindi...

Characterization of Spin1 genetrap mouse line. (A) Genomic organization of mouse Spindlin1 (Spin1). Spin1 exons 1–6 are depicted by white boxes. β-geo denotes the cassette containing the galactosidase/neomycin phosphotransferase fusion gene. (B) Genotyping results of wild type (+/+), Spin1 genetrap heterozygous (GT/+) and homozygous E18.5 fetuses (...

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MYC-tagged SERBP1 is co-immunoprecipitated with HA-tagged SPIN1. MYC-tagged SERBP1 was pulled down from HEK293T cells using MYC-antibody. HA-tagged SPIN1 was probed using HA-antibody. (DOCX)

Wild type SPIN1 and SPIN1 point mutant (Y155F) are expressed at similar level in HEK293T cells. Protein extracts were prepared from HEK293T cells expressing various constructs. Endogenous SERBP1 and MYC-SPIN1 were probed with SERBP1-antibody and MYC-antibody, respectively. Actin was included as loading control. (DOCX)

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This is the dataset of the 2-cell stage mouse embryo expressed genes updated in 2008.

During its growth phase, a mouse oocyte accumulates RNA that is the sole template for new protein synthesis in the transcriptionally silent interval between growth completion and transcriptional activation of the embryonic genome. Over this transcriptionally silent interval, almost half the quantity of RNA accumulated in the full-grown oocyte is de...

In recent years, the molecular underpinnings of the long-observed resemblance between neoplastic and immature tissue have begun to emerge. Genome-wide transcriptional profiling has revealed similar gene expression signatures in several tumor types and early developmental stages of their tissue of origin. However, it remains unclear whether such a r...

Frequency plots for all cancer types and all developmental time series.

Heatmap of enrichment p-values for gene sets that ranked among the 20 most enriched in the downregulated genes of either group 1, 2 or 3.

Raw data for the lung development validation time series (after RMA-normalization).

This shows a bimodal distribution with the left peak containing group 3 tumors, the right peak containing group 1 tumors and intermediate cases (group 2) falling in between.

Frequency plots for the top 450 differentially expressed genes in CRCC1 and CRCC2.

Frequency plots for all cancers and all time series after CC subtraction.

Heatmap of probability distribution slopes after CC subtraction (analogously to Figure 3).

Detailed annotation for all data sets used in this study.

Probability distribution plots and linear regression fits for all cancers and all time series.

The same data as additional data file 9, but after CC subtraction.

This 12-month-old, outdoor intact male cat (Fig. 1) was examined because of an untreated draining nasal lesion. The lesion. initially appeared as small punctures, and progressed ovcr 2 months to an ulcerated draining nodule that gradually enlarged to involve the whole muzzle, extending bilaterally to the medial canthi and having multiple drainage p...

Abstract— The clinical and histologic features of a 2-year-old intact male golden retriever with nodular dermatofibrosis and renal cystadenomas are described. The skin lesions failed to respond to antibiotic and glucocorticoid therapy. Abdominal ultrasound revealed a wedge-shaped area of hyperechoic mottled echogenicity at the cranial pole of the l...

In dissecting the molecules and molecular mechanisms that control mammalian oocyte-to-embryo transition, we found abundant transcripts representing developmentally regulated ERVs (endogenous retroviruses) in mouse oocyte and two-cell stage embryo cDNA libraries. These retrotransposons can act as alternative promoters and first exons for diverse gen...

What causes phenotypic variation? By now it is clear that phenotype is a result of the interaction between genotype and environment, in addition to variation not readily attributable to either. Epigenetic phenomena associated with phenotypic variation at the biochemical, cellular, tissue, and organism level are now well recognized and are likely to...

A comprehensive analysis of transposable element (TE) expression in mammalian full-grown oocytes reveals that LTR class III retrotransposons make an unexpectedly high contribution to the maternal mRNA pool, which persists in cleavage stage embryos. The most abundant transcripts in the mouse oocyte are from the mouse transcript (MT) retrotransposon...

The mechanisms causing persistence of embryonal cells that later give rise to tumors is unknown. One tumorigenic factor in the embryonal childhood tumor neuroblastoma is the MYCN protooncogene. Here we show that normal mice developed neuroblast hyperplasia in paravertebral ganglia at birth that completely regressed by 2 weeks of age. In contrast, g...

Excerpt In one sense, development of multicellular organismsbegins well before fertilization because the moleculesand controlling mechanisms that direct early development are put in place during oogenesis. The informationalcontent of a mammalian egg at fertilization is not only restricted to its DNA sequence, but also to various DNAand chromatin mo...

The transcriptome of the 2-cell mouse embryo was analyzed to provide insight into the molecular networks at play during nuclear reprogramming and embryonic genome activation. Analysis of ESTs from a 2-cell cDNA library identified nearly 4,000 genes, over half of which have not been previously studied. Transcripts of mobile elements, especially thos...

In one sense, development of multicellular organisms begins well before fertilization because the molecules and controlling mechanisms that direct early develop- ment are put in place during oogenesis. The informational content of a mammalian egg at fertilization is not only re- stricted to its DNA sequence, but also to various DNA and chromatin mo...

The elucidation of the molecular control of the initiation of mammalian embryogenesis is possible now that the transcriptomes of the full-grown oocyte and two-cell stage embryo have been prepared and analysed. Functional annotation of the transcriptomes using gene ontology vocabularies, allows comparison of the oocyte and two-cell stage embryo betw...

This chapter discusses the fertilization and activation of the embryonic genome. The full-grown oocyte arrested in prophase of the first meiotic division contains all of the molecules that are utilized to bridge the period of transcriptional silence that begins with the completion of oocyte growth. Under hormonal stimulation the full-grown oocyte b...

We have previously reported a strong correlation between poor prognosis in childhood neuroblastoma (NB) patients and high-level expression of the transmembrane efflux pump, Multidrug Resistance-associated Protein (MRP1), in NB tumour tissue. In this study, we inhibited the endogenous expression of MRP1 in 2 different NB tumour cell lines by stably...

To determine the efficacy of doxorubicin when used alone in inducing remission in cats with lymphosarcoma. Prospective multi-institutional study of naturally occurring disease. Cases were accrued from veterinary institutions in Australia and New Zealand after obtaining consent from informed owners. Cats were treated with doxorubicin every 3 weeks f...

Specific germline mutations in the RET proto-oncogene predispose to the familial cancer syndromes: multiple endocrine neoplasia (MEN) types 2A and 2B, and familial medullary thyroid carcinoma. Expression of the RET receptor tyrosine kinase is tightly restricted to tumours of neural crest origin, such as neuroblastoma, and neuroblastoma has been obs...

Point mutations, deletions, and recombinations of the RET proto-oncogene are associated with several inherited human diseases of neural crest-derived cells: Hirschsprung's disease, familial medullary thyroid carcinoma, and the multiple endocrine neoplasia (MEN) syndromes, types 2A and 2B. RET expression is restricted to normal and malignant cells o...

Microtubular reorganisation contributing to apoptotic morphology occurs in normal and neoplastic cells undergoing apoptosis induced by cytotoxic drugs [1-3]. The aim of this study was to correlate the changes in the microtubules (MTs) with behavior of the centrosome in apoptotic cells, and to see whether post-translational changes in tubulin occurr...

To characterise epidemiological and clinical findings, and diagnostic procedures undertaken, in cats with lymphosarcoma at a veterinary teaching hospital. Retrospective case study. Hospital records were reviewed for 7159 cats, sick or healthy, examined during a 10-year period (1984 to 1994). Sixty cats with lymphosarcoma were identified and classif...

A cat with weight loss, pyrexia and recurrent lethargy and depression was found to have pleural and peritoneal eosinophilic effusions, peripheral eosinophilia, eosinophilic lymphadenitis and a massively enlarged mesenteric lymph node. Visceral mast cell neoplasia was diagnosed after histopathological examination of a biopsy of the mass. Palliative...

Phthalocyanines are currently under investigation as photosensitizers for use in photodynamic therapy (PDT). We have been examining the efficacy of PDT in Domestic cats that have been treated for nasal and aural squamous cell carcinomas (SCC) using the photosensitizer aluminum phthalocyanine tetrasulphonate (AIPCS,) (Porphyrin Products, Logan, UT)....

Sixteen cats with malignant tumors (10 carcinomas, 6 sarcomas) of the nasal cavity and paranasal sinuses were treated with curative intent by radiotherapy. Clinical stating was based on radiographic findings, using the tumor, node, metastasis classification system of the World Health Organization. Irradiation was done with a telecobalt-60 unit (13...

Eighteen random-bred cats with a total of 19 nasal or aural squamous cell carcinomas were treated with photodynamic therapy, using aluminum phthalocyanine tetrasulfonate as the photosensitizer. Cats were irradiated at power densities of 100 mW/cm2 and energy densities of 100 J/cm2. Successful outcome was obtained in 10 tumors after 1 treatment, and...

The pattern of distribution of cytokeratin (CK) intermediate filaments can be used to characterize subsets of epithelial tissues. The purpose of the study was to examine the CK expression of feline pinna skin. Six normal feline pinnae were routinely processed in formalin. An immunohistochemical method was used to stain the pinnae with 8 commerciall...

A dog was examined because of anorexia and development of submandibular, sternal, and forelimb edema. Physical examination revealed engorged jugular veins and engorged blood vessels of the conjunctivae and nictitating membranes. Thoracic radiography revealed pleural and pericardial effusions, later identified as chyle. Contrast angiography revealed...