Anne Godfroy

Anne Godfroy
Institut Français de Recherche pour l'Exploitation de la Mer | Ifremer · Laboratory of Microbiology of Extreme Environments (LM2E)

PhD

About

118
Publications
16,451
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
2,380
Citations
Citations since 2017
27 Research Items
969 Citations
2017201820192020202120222023050100150
2017201820192020202120222023050100150
2017201820192020202120222023050100150
2017201820192020202120222023050100150
Introduction
Anne Godfroy currently works at the Laboratory of Microbiology of Extreme Environments (LM2E), Institut Français de Recherche pour l'Exploitation de la Mer. Anne does research in Microbiology an Microbial ecology in Marine extreme environnement
Additional affiliations
Education
October 1985 - January 1989
October 1984 - June 1985
University Joseph Fourier - Grenoble 1
Field of study
  • Microbial Nitrogen fixation

Publications

Publications (118)
Article
This paper reports on the genome analysis of strain F29 representing a new species of the genus Thermosulfurimonas. This strain, isolated from the Lucky Strike hydrothermal vent field on the Mid-Atlantic Ridge, is able to grow by disproportionation of S0 with CO2 as a carbon source. Strain F29 possesses a genome of 2,345,565 bp, with a G+C content...
Article
Full-text available
Recent studies have shown the presence of an abiotic electrical current across the walls of deep-sea hydrothermal chimneys, allowing the growth of electroautotrophic microbial communities. To understand the role of the different phylogenetic groups and metabolisms involved, this study focused on electrotrophic enrichment with nitrate as electron ac...
Article
Full-text available
Carbon budgets of hydrothermal plumes result from the balance between carbon sinks through plume chemoautotrophic processes and carbon release via microbial respiration. However, the lack of comprehensive analysis of the metabolic processes and biomass production rates hinders an accurate estimate of their contribution to the deep ocean carbon cycl...
Article
Full-text available
Deep-sea hydrothermal vents are extreme and complex ecosystems based on a trophic chain. We are still unsure of the identities of the first colonizers of these environments and their metabolism, but they are thought to be (hyper)thermophilic autotrophs. Here we investigate whether the electric potential observed across hydrothermal chimneys could s...
Article
Removal of reducing equivalents is an essential catabolic process for all microorganisms to maintain their internal redox balance. The electron disposal by chemoorganotrophic Thermococcales generates H2 by proton reduction or H2S in presence of S0. Although in the absence of S0 growth of these (hyper)thermopiles was previously described to be H2-li...
Preprint
Recent studies have shown the presence of an abiotic electrical current across the walls of deep-sea hydrothermal chimneys, allowing the growth of electroautotrophic microbial communities. To understand the role of the different phylogenetic groups and metabolisms involved, this study focused on an electrotrophic enrichment, with nitrate as electro...
Article
A novel thermophilic, microaerophilic and anaerobic, hydrogen- sulphur- and thiosulphate-oxidising bacterium, designated MO1340T, was isolated from a deep-sea hydrothermal chimney collected from the Lucky Strike hydrothermal vent field on the Mid-Atlantic Ridge. Cells were short, motile rods of 1.4 - 2.2 µm length and 0.5 - 0.8 µm width. Optimal gr...
Preprint
Deep-sea hydrothermal vents are extreme and complex ecosystems based on a trophic chain. We are still unsure of the first colonizers of these environments and their metabolism, but they are thought to be (hyper)thermophilic autotrophs. Here we investigate whether the electric potential observed across hydrothermal chimneys could serve as an energy...
Article
Deep-sea hydrothermal vents are inhabited by complex communities of microbes and their viruses. Despite the importance of viruses in controlling the diversity, adaptation and evolution of their microbial hosts, to date, only eight bacterial and two archaeal viruses isolated from abyssal ecosystems have been described. Thus, our efforts focused on g...
Article
Full-text available
To study the role of exoelectrogens within the trophic network of deep-sea hydrothermal vents, we performed successive subcultures of a hyperthermophilic community from a hydrothermal chimney sample on a mix of electron donors in a microbial fuel cell system. Electrode (the electron acceptor) was swapped every week to enable fresh development from...
Article
Full-text available
8-oxodeoxyguanosine (8-oxodG), a major oxidised base modification, has been investigated to study its impact on DNA replication in hyperthermophilic Archaea. Here we show that 8-oxodG is formed in the genome of growing cells, with elevated levels following exposure to oxidative stress. Functional characterisation of cell-free extracts and the DNA p...
Article
Full-text available
The mixing zone between high temperature hydrothermal fluids and seawater produces redox gradients, promoting the development of unique ecosystems based on chemotrophy. The structure of microbial communities depends on their environment, which can vary according to space and time. Hydrothermal circulation within the oceanic crust determines the che...
Article
Full-text available
This study provides an analysis of vesicomyid bivalve–symbiont community distribution across cold seep and hydrothermal vent areas in the Guaymas Basin (Gulf of California, Mexico). Using a combination of morphological and molecular approaches including fluorescent in situ hybridization (FISH), and electronic microscopy observations, vesicomyid cla...
Article
While more and more investigations are done to study hyperthermophilic exoelectrogenic communities from environments, none have been performed yet on deep-sea hydrothermal vent. Samples of black smoker chimney from Rainbow site on the Atlantic mid-oceanic ridge have been harvested for enriching exoelectrogens in microbial electrolysis cells under h...
Preprint
While more and more investigations are done to isolate hyperthermophilic exoelectrogenic communities from environments, none have been performed yet on deep-sea hydrothermal vent. Samples of black smoker chimney from Rainbow site on the Atlantic mid-oceanic ridge have been harvested for enriching exoelectrogens in microbial electrolysis cells under...
Book
How can the evolutionary success of prokaryotes be explained ? How did they manage to survive conditions that have fluctuated, with drastic events over 3.5 billion years ? Which significant metabolisms and mechanisms have appeared over the course of evolution that have permitted them to survive the most inhospitable conditions from the physicochemi...
Article
Full-text available
Insights into the factors controlling fluid circulation through the crust and the nature of fluid venting at the seafloor are first steps in understanding their effect on ocean properties and climate change. New data on the seafloor morphology, sub-surface sedimentary stratification, and water column of the sedimented Southern Trough hydrothermal f...
Chapter
How can the evolutionary success of prokaryotes be explained? How did they manage to survive conditions that have fluctuated, with drastic events over 3.5 billion years? Which significant metabolisms and mechanisms have appeared over the course of evolution that have permitted them to survive the most inhospitable conditions from the physicochemica...
Article
How can the evolutionary success of prokaryotes be explained ? How did they manage to survive conditions that have fluctuated, with drastic events over 3.5 billion years ? Which significant metabolisms and mechanisms have appeared over the course of evolution that have permitted them to survive the most inhospitable conditions from the physicochemi...
Article
Full-text available
In the Guaymas Basin, the presence at a few tens of kilometers of cold seeps and hydrothermal vents coupled with comparable sedimentary settings and depths offer a unique opportunity to assess and compare the microbial community composition of these deep-sea ecosystems. The microbial diversity in sediments from three cold seep and two hydrothermal...
Poster
Full-text available
In the 1980s was discovered in total darkness an unknown and luxuriant biocenosis, developed around vertical chimneys: the deep hydrothermal vents. Their observation have shown a complex ecosystems colonized by highly diversified (hyper)thermophilic microbial populations growing within dense biofilms. The primary production of organic matter would...
Article
Full-text available
Sitting at ~5 000 m water depth on the Congo-Angola margin and ~ 760 km offshore of the West African coast, the recent lobe complex of the Congo deep-sea fan receives large amounts of fluvial sediments (3-5% organic carbon). This organic-rich sedimentation area harbors habitats with chemosynthetic communities similar to those of cold seeps. In this...
Article
Full-text available
Members of the order Thermococcales are common inhabitants of high-temperature hydrothermal vent systems (black smokers) that are represented in clone libraries mostly by isolates from the Thermococcus genus. We report the complete sequence of a novel species from the Pyrococcus genus, P. kukulkanii strain NCB100, which has been isolated from a fla...
Article
Rich animal and microbial communities have been found at deep-sea hydrothermal vents. Although the biogeography of vent macrofauna is well understood, the corresponding knowledge about vent microbial biogeography is lacking. Here, we apply the multilocus sequence analysis (MLSA) to assess the genetic variation of 109 Sulfurimonas strains with ⩾98%...
Article
Full-text available
Here, we present the draft genome sequences of two thermophilic Marinitoga strain members of the Thermotogales order, Marinitoga camini DV1155 and Marinitoga camini DV1197. These strains were isolated from deep-sea hydrothermal vents of the Mid-Atlantic Ridge.
Poster
Full-text available
Electroactive microorganisms may play a key role in biogeochemical cycles and, consequently, in the functioning of ecosystems. They preferentially grow in biofilms composed of diverse microbial species, forming communities with strong inter-species interactions (mutualisms) and a complex trophic chain. Recently, a new type of mutualism called “elec...
Article
Full-text available
Cultivation in a bioreactor of immobilized deep-sea hydrothermal microbial community was tested in order to assess the stability and reactivity of this new system. A community composed of 8 hydrothermal strains was entrapped in a polymer matrix that was used to inoculate a continuous culture in a gas-lift bioreactor. The continuous culture was perf...
Article
Full-text available
A novel hyperthermophilic, piezophilic anaerobic archaeon, designated as NCB100T, was isolated from a hydrothermal vent flange fragment collected in the Guaymas basin at the hydrothermal vent site named "Rebecca's Roost" at a depth of 1997 m. Enrichment and isolation were done at 100°C under atmospheric pressure. Cells of strain NCB100T are highly...
Article
The presently active region of the Congo deep-sea fan (around 330 000 km2), called the terminal lobes or lobe complex, covers an area of 2500 km2 at 4700–5100 m water depth and 750–800 km offshore. It is a unique sedimentary area in the world ocean fed by a submarine canyon and a channel-levee system which presently deliver large amounts of organic...
Article
Full-text available
Aims: The aims of this study were (i) to develop a protocol for the entrapment of anaerobic (hyper)thermophilic marine microorganisms; (ii) to test the use of the chosen polymers in a range of physical and chemical conditions and; (iii) to validate the method with batch cultures. Methods and results: The best conditions for immobilization were o...
Research
Full-text available
Conference paper #OS43A-2031 AGU 2015 To study the hydrothermal forcing on microbial colonization, and impacts on the oceanic crust alteration, an integrated study was led at the Tour Eiffel hydrothermal site (Lucky Strike hydrothermal field, 37°N, MAR). We benefited from an annual survey between 2009 and 2011 of temperatures, along with sampling...
Article
Full-text available
To explore the capability of basaltic glass to support the growth of chemosynthetic microorganisms, complementary in situ and in vitro colonization experiments were performed. Microbial colonizers containing synthetic tholeitic basaltic glasses, either enriched in reduced or oxidized iron, were deployed off-axis from the Mid Atlantic Ridge on surfa...
Conference Paper
To study the hydrothermal forcing on microbial colonization, and impacts on the oceanic crust alteration, an integrated study was led at the Tour Eiffel hydrothermal site (Lucky Strike hydrothermal field, 37°N, MAR). We benefited from an annual survey between 2009 and 2011 of temperatures, along with sampling of focused and diffused fluids for chem...
Article
Full-text available
Understanding the ecological processes and connectivity of chemosynthetic deep-sea ecosystems requires comparative studies. In the Guaymas Basin (Gulf of California, Mexico), the presence of seeps and vents in the absence of a biogeographic barrier, and comparable sedimentary settings and depths offers a unique opportunity to assess the role of eco...
Chapter
Extreme physicochemical conditions (low and high temperatures, high salinity, low and high pH, high hydrostatic pressure, etc.) existing on Earth are compatible with the occurrence of microbial life. The diversity and metabolic features of microbial trophic groups inhabiting extreme environments (cold, hot, saline, acidic, alkaline, and deep marine...
Article
Full-text available
A novel, anaerobic, chemoorganotrophic bacterium, designated strain Ra1766HT, was isolated from sediments of the Guaymas basin (Gulf of California, Mexico) at 2002 m depth. Cells were thin, motile, Gram-positive flexible rods forming terminal endospores. Strain Ra1766HT grew at temperatures between 25-45 °C (optimum 30 °C), pH 6.7-8.1 (optimum 7.5)...
Article
Full-text available
Understanding the ecological processes and connectivity of chemosynthetic deep-sea ecosystems requires comparative studies. In the Guaymas Basin (Gulf of California, Mexico), the presence of seeps and vents in the absence of biogeographic barrier, comparable sedimentary settings and depths offers a unique opportunity to assess the role of ecosystem...
Article
Full-text available
Active hydrothermal chimneys host diverse microbial communities exhibiting various metabolisms including those involved in various biogeochemical cycles. To investigate microbe-mineral-fluid interactions in hydrothermal chimney and the driver of microbial diversity, a cultural approach using a gas-lift bioreactor was chosen. An enrichment culture w...
Article
Full-text available
In the Sonora Margin cold seep ecosystems (Gulf of California), sediments underlying microbial mats harbor high biogenic methane concentrations, fuelling various microbial communities such as abundant lineages of Anaerobic Methanotrophs (ANME). However biodiversity, distribution and metabolism of the microorganisms producing this methane remain poo...
Article
Les écosystèmes hydrothermaux sous-marins profonds hébergent des communautés microbiennes d'une étonnante diversité tant par leur métabolisme que par leur capacité à se développer dans des conditions extrêmes, notamment de température et de pression. Ces microorganismes sont ainsi de bons candidats pour les biotechnologies.
Article
Full-text available
To assess the production and the export of particulate and biological material in deep-sea hydrothermal vents, four moorings with sediment traps and current meters were deployed for 24 days in the Azores Triple Junction (ATJ) region. They were deployed along an axis starting at the base of a hydrothermal vent chimney and ending 1000 m away. The par...
Article
The subsurface realm is colonized by microbial communities to depths of 41000 meters below the seafloor (m.b.sf.), but little is known about overall diversity and microbial distribution patterns at the most profound depths. Here we show that not only Bacteria and Archaea but also Eukarya occur at record depths in the subseafloor of the Canterbury B...
Conference Paper
Full-text available
Biological patterns driven by extreme environmental settings: a comparison between vent and seep macrofaunal communities in the Guaymas basin Portail Marie1, Karine Olu1, Lénaick Menot1, Yves Gélinas3, Jean-Claude Caprais1, Stanislas Dubois2, Anne Godfroy1, Jozée Sarrazin1 1 Institut Carnot Ifremer EDROME, Centre de Bretagne, REM/EEP, Laboratoire...
Conference Paper
Full-text available
It was recently discovered (Kim et al., 2010) that some Thermococcus strains (T. onnurineus NA1, T. gammatolerans EJ3T, and Thermococcus sp. DS-1, Ch5 and DT4), isolated from geographically remote deep-sea hydrothermal vents of the Atlantic and Pacific Oceans, can grow at the expense of hydrogen production from formate. Based on comparison of the T...
Article
TET aminopeptidases assemble as large homo-dodecameric complexes. The reason why prokaryotic genomes often encode a diverse set of TET peptidases homologues remains unclear. In the archaeon Pyrococcus horikoshii, PhTET1, PhTET2 and PhTET3 homo-oligomeric particles have been proposed to work in concert to breakdown intracellular polypeptides. When c...
Article
Full-text available
Subsurface sediments of the Sonora Margin (Guaymas Basin), located in proximity of active cold seep sites were explored. The taxonomic and functional diversity of bacterial and archaeal communities were investigated from 1 to 10 meters below the seafloor. Microbial community structure and abundance and distribution of dominant populations were asse...
Article
Full-text available
Next Generation Sequencing (NGS) opens up exciting possibilities for improving our knowledge of the environmental microbial diversity, allowing rapid and cost-effective identification of both cultivated and uncultivated microorganisms. However, library preparation, sequencing and analysis of the results can provide inaccurate representation of the...
Article
Full-text available
A novel obligately anaerobic, extremely thermophilic, organotrophic bacterium, strain Rift-s3T, was isolated from a deep-sea sample containing Riftia pachyptila sheath from Guaymas Basin, Gulf of California. Cells of new isolate were rods 0.3-0.8 μm in width and 1.5-10 μm in length surrounded by a sheath-like structure (toga). Strain Rift-s3T grew...
Article
Full-text available
The subsurface realm is colonized by microbial communities to depths of >1000 meters below the seafloor (m.b.sf.), but little is known about overall diversity and microbial distribution patterns at the most profound depths. Here we show that not only Bacteria and Archaea but also Eukarya occur at record depths in the subseafloor of the Canterbury B...
Conference Paper
Full-text available
In the dark ocean, microbial communities must thrive on organic matter coming from the photic zone or on redox reaction through chemosynthesis. Chemosynthetic microorganisms need to couple oxidized compounds, which are abundant in seawater, to reduced compounds. Near ocean ridges, newly formed basalt is enriched in these compounds. Even if the bioa...
Article
Full-text available
The Sonora Margin cold seeps present on the seafloor a patchiness pattern of white microbial mats surrounded by polychaete and gastropod beds. These surface assemblages are fuelled by abundant organic inputs sedimenting from the water column and upward-flowing seep fluids. Elevated microbial density was observed in the underlying sediments. A previ...
Article
Full-text available
In Archaea, the proteins involved in the genetic information processing pathways, including DNA replication, transcription, and translation, share strong similarities with those of eukaryotes. Characterizations of components of the eukaryotic-type replication machinery complex provided many interesting insights into DNA replication in both domains....
Article
Full-text available
Oceanic basalts host diverse microbial communities with various metabolisms involved in C, N, S, and Fe biogeochemical cycles which may contribute to mineral and glass alteration processes at, and below the seafloor. In order to study the microbial colonization on basaltic glasses and their potential biotic/abiotic weathering products, two coloniza...
Conference Paper
Full-text available
Hematite, the most abundant mineral in Iron Oxide Copper Gold (IOCG) deposits in the Gawler Craton of South Australia, may incorporate significant amounts of U, W, Mo and radiogenic Pb. These elements are concentrated in hematite grains which display either oscillatory and/or sectorial compositional zoning, or are porous. The presence of U and Pb r...
Article
Full-text available
Hydrothermally active sediments are highly productive, chemosynthetic areas which are characterized by the rapid turnover of particulate organic matter under extreme conditions in which ammonia is liberated. These systems might be suitable habitats for anaerobic ammonium oxidizing (anammox) bacteria but this has not been investigated in detail. Her...
Article
Full-text available
A novel obligately anaerobic, non-spore-forming, rod-shaped mesophilic, halophilic, Gram-stain-negative bacterium, was isolated from sediments of Guaymas Basin. The strain, designated Ra1766G1(T), grew at 20-40 degrees C (optimum, 30-35 degrees C) and at pH 6.0-8.0 (optimum, pH 6.5-7.5). It required 0.5-7.5 % NaCl (optimum, 2-3 %) for growth. Sulfa...
Article
Full-text available
Tetrahedral (TET) aminopeptidases are large polypeptide destruction machines present in prokaryotes and eukaryotes. Here, the rules governing their assembly into hollow 12-subunit tetrahedrons are addressed by using TET2 from Pyrococcus horikoshii (PhTET2) as a model. Point mutations allowed the capture of a stable, catalytically active precursor....