Anne Dudeck

Anne Dudeck
Otto-von-Guericke-Universität Magdeburg | OvGU · Institut für Molekulare und Klinische Immunologie

Prof. Dr., PhD

About

66
Publications
14,502
Reads
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2,358
Citations
Introduction
My research group addresses the intercellular communication of innate immune cells. Using intravital multiphoton microscopy we monitor immune cells at work in real time and follow immune responses during inflammation until regeneration of the tissue. Our main focus is the function of mast cells (MCs) in immune responses and inflammation. We have shown that MCs exhibit critical roles as tissue resident sentinel cells that initiate innate immune responses and impact on adaptive immunity.
Additional affiliations
August 2016 - present
Otto-von-Guericke-Universität Magdeburg
Position
  • Professor
April 2009 - July 2016
Technische Universität Dresden
Position
  • PostDoc Position
September 2005 - March 2009
Charité Universitätsmedizin Berlin
Position
  • PostDoc Position
Education
October 1995 - October 2000
University of Leipzig, Faculty of Biosciences, Pharmacy and Psychology
Field of study
  • Biochemistry

Publications

Publications (66)
Article
Full-text available
A prominent feature of sensitizing environmental compounds that cause allergic contact dermatitis is the rapid induction of an innate inflammatory response that seems to provide danger signals for efficient T cell priming. We generated mouse models of mast cell deficiency, mast cell-specific gene inactivation, and mast cell reporter mice for intrav...
Article
Full-text available
Mast cells (MCs) and dendritic cells (DCs) are essential innate sentinels populating host-environment interfaces. Using longitudinal intravital multiphoton microscopy of DC GFP /MC RFP reporter mice, we herein provide in vivo evidence that migratory DCs execute targeted cell-to-cell interactions with stationary MCs before leaving the inflamed skin...
Article
BACKGROUND: Mast cells (MCs) are best known as key effector cells of allergic reactions but they also play an important role in host defense against pathogens. Despite increasing evidence for a critical impact of MCs on adaptive immunity, the underlying mechanisms are poorly understood. OBJECTIVE: Here, we monitored MC intercellular communication...
Article
Full-text available
Mast cells (MCs) contact hypersensitivity responses, but the mode of MC activation is not yet understood. Hoppe et al. show, that MCs trigger an immediate edema formation and neutrophil infiltration by sensing cell stress concomitantly via the ATP/P2X7 and IL-33/ST2 pathway.
Article
Tissue resident mast cells (MCs) rapidly initiate neutrophil infiltration upon inflammatory insult, yet the molecular mechanism is still unknown. Here, we demonstrated that MC-derived tumor necrosis factor (TNF) was crucial for neutrophil extravasation to sites of contact hypersensitivity-induced skin inflammation by promoting intraluminal crawling...
Article
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There is evidence that mast cells contribute to inflammation induced by hemorrhagic shock, severe tissue injury or sepsis. Mast cells are highly responsive to alarm signals generated after trauma, and release many inflammatory mediators including interleukin-6, a key mediator of posttraumatic inflammation. An overwhelming posttraumatic inflammation...
Article
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T helper (Th) cells provide immunity to pathogens but also contribute to detrimental immune responses during allergy and autoimmunity. Th2 cells mediate asthmatic airway inflammation and Th1 cells are involved in the pathogenesis of multiple sclerosis. T cell activation involves complex transcriptional networks and metabolic reprogramming, which en...
Article
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Nitric oxide (NO) is an important antimicrobial effector but also prevents unnecessary tissue damage by shutting down the recruitment of monocyte-derived phagocytes. Intracellular pathogens such as Leishmania major can hijack these cells as a niche for replication. Thus, NO might exert containment by restricting the availability of the cellular nic...
Article
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Mast cells are important tissue resident sensor and effector immune cells, but play also a major role in osteoporosis development. Mast cells are increased in numbers in the bone marrow of postmenopausal osteoporotic patients, and mast cell‐deficient mice are protected from ovariectomy (OVX)‐induced bone loss. In this study, we showed that mast cel...
Article
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IL-33 and ATP are alarmines which are released upon damage of cellular barriers or are actively secreted upon cell stress. Due to high density expression of the IL-33 receptor T1/ST2 (IL-33R), and the ATP receptor P2X7, mast cells (MCs) are one of the first highly sensitive sentinels recognizing released IL-33 or ATP in damaged peripheral tissues....
Article
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The function of T cells is critically dependent on their ability to generate metabolic building blocks to fulfil energy demands for proliferation and consecutive differentiation into various T helper (Th) cells. Th cells then have to adapt their metabolism to specific microenvironments within different organs during physiological and pathological i...
Article
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Mast cells (MCs) are best-known as key effector cells of immediate-type allergic reactions that may even culminate in life-threatening anaphylactic shock syndromes. However, strategically positioned at the host–environment interfaces and equipped with a plethora of receptors, MCs also play an important role in the first-line defense against pathoge...
Preprint
Full-text available
Mast cells (MCs) are best known as key effector cells of immediate type allergic reactions that may even culminate in life-threatening anaphylactic shock syndromes. However, strategically positioned at host-environment interfaces and equipped with a plethora of receptors, MCs also play an important role in the first line defense against pathogens....
Article
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C-type lectin receptors (CLRs) are pattern recognition receptors that are crucial in the innate immune response. The gastrointestinal tract contributes significantly to the maintenance of immune homeostasis; it is the shelter for billions of microorganisms including many genera of Lactobacillus sp. Previously, it was shown that host-CLR interaction...
Article
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Although mast cells (MCs) are known as key drivers of type I allergic reactions, there is increasing evidence for their critical role in host defense. MCs not only play an important role in initiating innate immune responses, but also influence the onset, kinetics, and amplitude of the adaptive arm of immunity or fine-tune the mode of the adaptive...
Preprint
Full-text available
Although Mast cells are known as key drivers of type I allergic reactions, there is increasing evidence for their critical role in host defense. MCs do not only play an important role in initiating innate immune responses, but also influence the onset, kinetic and amplitude of the adaptive arm of immunity, or fine-tune the mode of the adaptive reac...
Article
Mast cells (MCs) are tissue-resident hematopoietic cells intensely studied for their role as effectors in allergic immune responses. Yolk sac-derived embryonic MCs first populate tissues and are later replaced by definitive MCs. We show that definitive MC progenitors expand locally in skin and form clonal colonies that cover stable territories. In...
Article
Full-text available
Mast cells (MCs) are important sensor and effector cells of the immune system that are involved in many physiological and pathological conditions. Increasing evidence suggests that they also play an important role in bone metabolism and bone disorders. MCs are located in the bone marrow and secrete a wide spectrum of mediators, which can be rapidly...
Article
Tissue resident mast cells (MCs) rapidly initiate neutrophil infiltration upon inflammatory insult, yet the molecular mechanism is still unknown. Here, we demonstrated that MC-derived tumor necrosis factor (TNF) was crucial for neutrophil extravasation to sites of contact hypersensitivity-induced skin inflammation by promoting intraluminal crawling...
Article
Mast cells (MCs), which are well known for their effector functions in TH2-skewed allergic and also autoimmune inflammation, have become increasingly acknowledged for their role in protection of health. It is now clear that they are also key modulators of immune responses at interface organs, such as the skin or gut. MCs can prime tissues for adequ...
Article
Full-text available
The virulence of intracellular pathogens such as Leishmania major (L. major) relies largely on their ability to undergo cycles of replication within phagocytes, release, and uptake into new host cells. While all these steps are critical for successful establishment of infection, neither the cellular niche of efficient proliferation, nor the spread...
Data
mKikume expression in BM cells allows identification of photoconverted phagocytes after 48h of photoconversion. Ubiquitous mKikume expressing mice were infected with non-fluorescent L. major wild type. Photoconversion in the mouse ear was performed 48h prior to analysis. Control samples were photoconverted 0 h prior to analysis or not photoconverte...
Data
Multiparameter microscopy analysis strategy. (A) Example MELC images of different aligned fluorescence channels of an infected tissue site. A nonspecific IgG staining of the same site is shown for comparison. (B) Deconvolved and aligned image stacks of CD11b, CD45 and CD54 stainings were used separately as membrane input, the corresponding propidiu...
Data
A quantitative in vitro analysis approach for parasite cell-to-cell transfer. (A) Analysis of expression levels of uninfected CD11c, F4/80 and MHC class II in bone marrow-derived dendritic cell (green histograms) and macrophage (blue histograms) cultured. Data are representative of two independent experiments. (B) Analysis of CD11c versus F4/80 exp...
Data
Intravital 2-photon microscopy of cell-to-cell transfer of L. major (red) from recipient CD11c-EYFP cells (green) into newly recruited adoptively transferred cells (blue). CD11c-EYFPtg mice were infected in the ear for 16 weeks with monofluorescent L. major DsRed, ECFP-expressing bome marrow cells were adoptively transferred and the site of infecti...
Data
Grid control of the flow-cytometry based proliferation analysis approach. (A) Grid illumination approach for scrambled photoconversion. Left panel, illumination grid (transmitted light image). Middle panel, intravital 2-photon image of a scramble-photoswitched LmSWITCH-infected site in the ear, an image aligned to the grid showing photoswitched 80...
Data
Synchronization of newly recruited cell arrival. (A) Flow cytometry analysis of CD45.1 mice infected with LmSWITCH, adoptively transferred 2 or 5 days before analysis with C57BL/6 (CD45.2) bone marrow cells. Gating strategy on CD45.2+ (newly recruited) and CD45.1+ (steady state) cells, infected and non-infected. (B) Quantification of newly recruite...
Data
Intravital 2-photon microscopy demonstration of de novo infection of newly recruited phagocytes by L. major juxtapositioned to a CD11c+ cell. (A) Two examples de novo infection experiments of newly recruited cells (blue) by L. major (red) initially juxtapositioned to a CD11c+ host cell (green). Images are selected projections of 10–13 slices of 3 μ...
Data
Optimization of RACE conditions for single cell detection. Deconvolved 400 x 400 x 8 micron stacks were segmented with the RACE settings indicated. Three infection sites from different mice (Site1-Site3) and two Z planes per site (ZPl1-ZPl2) were converted into flow cytometry datasets and analyzed as described in the supplementary methods (see S1 T...
Data
Time lapse videomicroscopy of intraperitoneal macrophages infected for 24 h with fluorescently labeled L. major. Three examples of cell-to-cell transfer between two phagocytes are shown. (MOV)
Article
Full-text available
Innate inflammatory responses are crucial for induction and regulation of T cell and antibody responses. Mast cell (MC)-deficient Kit mutant mice showed impaired adaptive immunity, suggesting that MCs provide essential adjuvant activities, and pharmacological MC activation was proposed as a new adjuvant principle. However, the Kit mutations result...
Article
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The neurobeachin-like 2 protein (Nbeal2) belongs to the family of beige and Chediak-Higashi (BEACH) domain proteins. Loss-of-function mutations in the human NBEAL2 gene or Nbeal2 deficiency in mice cause gray platelet syndrome, a bleeding disorder characterized by macrothrombocytopenia, splenomegaly, and paucity of α-granules in megakaryocytes and...
Article
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Mast cells, important sensor and effector cells of the immune system, may influence bone metabolism as their number is increased in osteoporotic patients. They are also present during bone fracture healing with currently unknown functions. Using a novel c-Kit-independent mouse model of mast cell deficiency, we demonstrated that mast cells did not a...
Article
Full-text available
Cerebral toxoplasmosis is characterized by activation of brain resident cells and recruitment of specific immune cell subsets from the periphery to the central nervous system (CNS). Our studies revealed that the rapidly invaded Ly6G⁺ neutrophil granulocytes are an early non-lymphoid source of interferon-gamma (IFN-γ), the cytokine known to be the m...
Article
Full-text available
The IL-1R family member IL-33R mediates Fcε-receptor-I (FcεRI)-independent activation of mast cells leading to NF-κB activation and consequently the production of cytokines. IL-33 also induces the activation of MAPKs, such as p38. We aimed to define the relevance of the p38-targets, the MAPK-activated protein kinases 2 and 3 (MK2 and MK3) in IL-33-...
Article
The maintenance and modulation of cutaneous mast cell (MC) numbers is held to be important for skin immune responses to allergens and pathogens. The increase of MC numbers in the skin is achieved by proliferation and the differentiation of precursor to mature MCs. Fibroblast-derived SCF is thought to be the major skin MC growth factor and it potent...
Article
Full-text available
Mast cells and basophils are innate immune cells with overlapping functions that contribute to anti-helminth immunity. Mast cell function during helminth infection was previously studied using mast cell-deficient Kit-mutant mice that display additional mast cell-unrelated immune deficiencies. Here, we use mice that lack basophils or mucosal and con...
Article
Mast cells (MCs) are pro-inflammatory sensor and effector cells of the immune system. MCs seem to play a role in bone metabolism, because patients with systemic mastocytosis develop osteoporosis. MCs are present in the fracture callus during bone healing, however, their function has not yet been investigated. Here, we examined the role of MCs in th...
Article
Full-text available
Graphical Abstract Highlights d TNF release by mast cells is required for efficient CD8 + T cell responses to haptens d Mast cell TNF promotes the migration and maturation of CD8 + DCs specifically d Mast cell TNF distinctly boosts the CD8 + T-cell-priming efficiency of CD8 + DCs In Brief The impact of mast cells on T-cell-dominated adaptive immuni...
Article
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NF-κB activation depends on the IKK complex consisting of the catalytically active IKK1 and 2 subunits and the scaffold protein NEMO. Hitherto, IKK2 activation has always been associated with IκBα degradation, NF-κB activation, and cytokine production. In contrast, we found that in SCF-stimulated primary bone marrow-derived mast cells (BMMCs), IKK2...
Article
Full-text available
Objectives Recently, mast cell functions in autoimmune disorders have been discussed controversially. Mast cell deficient Kit mutant mice rendered resistant to K/BxN serum transfer arthritis, whereas a genetic model of mast cell deficiency independent of Kit mutation exposed full susceptibility. This debate might lead to the assumption of mast cell...
Article
Full-text available
Allergic contact dermatitis and its animal model, contact hypersensitivity (CHS), are T cell–mediated inflammatory skin diseases induced by contact allergens. Though numerous cellular and molecular players are known, the mechanism of chemical-induced sensitization remains poorly understood. Here, we identify neutrophils as crucial players in the se...
Article
Full-text available
Over the past decades, research on in vivo functions of mast cells has largely relied on kit-mutant mouse strains. Recently, new mouse models for investigation of mast cell functions based on the Cre/loxP recombination system have been published and results in these new models challenged findings of previous studies in kit-mutant mice. Herein we de...
Article
Full-text available
The immunoglobulin E (IgE)-mediated mast cell (MC) response is central to the pathogenesis of type I allergy and asthma. IκB kinase 2 (IKK2) was reported to couple IgE-induced signals to MC degranulation by phosphorylating the SNARE protein SNAP23. We investigated MC responses in mice with MC-specific inactivation of IKK2 or NF-κB essential modulat...
Article
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Neutrophil recruitment is an important early step in controlling tissue infections or injury. Here, we report that this influx depends on both tissue-resident mast cells and macrophages. Mice with mast cell deficiency recruit reduced numbers of neutrophils in the first few hours of intraperitoneal lipopolysaccharide (LPS) stimulation. Conversely, i...
Article
Mast cells (MCs) play an important role in the regulation of protective adaptive immune responses against pathogens. However, it is still unclear whether MCs promote such host defense responses via direct effects on T cells or rather by modifying the functions of antigen-presenting cells. To identify the underlying mechanisms of the immunoregulator...
Article
Please cite this paper as: Immature mast cells exhibit rolling and adhesion to endothelial cells and subsequent diapedesis triggered by E- and P-selectin, VCAM-1 and PECAM-1. Experimental Dermatology 2010; 19: 424–434. Abstract: Mast cell numbers are markedly increased at sites of chronic inflammation. However, the underlying mechanisms of mast cel...
Article
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Members of the Toll/interleukin-1 receptor (TIR) family are of importance for host defense and inflammation. Here we report that the TIR-family member interleukin-33R (IL-33R) cross-activates the receptor tyrosine kinase c-Kit in human and murine mast cells. The IL-33R-induced activation of signal transducer and activator of transcription 3 (STAT3)...
Article
Mast cells (MCs) are important effector cells in host defense against bacteria. In the course of a bacterial infection, MCs can be activated by various mechanisms, i.e. bacterial toxins, endogenously produced infection-associated peptides or via complement receptors, fimbrial adhesion molecules and toll-like receptors (TLRs). While some of these me...
Article
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The chronic inflammatory skin disease psoriasis is characterized by prominent skin infiltration by neutrophils and microabscess formation. The adhesion of leukocytes and subsequent transmigration through the activated endothelium is one prerequisite for the accumulation of these cells in skin. In recent studies, the human Thy-1 (CD90) was character...