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Introduction
Additional affiliations
September 1994 - March 2016
Position
- Senior Researcher
Description
- Host cytokine response to Salmonella infection. Chicken immune factors of genetic resistance to Salmonella intestinal carriage. Phenotypic traits of chicken susceptibility to avian influenza virus. Structure, and antibacterial activity of avian defensins.
September 1994 - March 2016
September 1994 - March 2016
Publications
Publications (61)
Divergent evolution for more than 310 million years has resulted in an avian immune system that is complex and more compact than that of primates, sharing much of its structure and functions. Not surprisingly, well conserved ancient host defense molecules, such as defensins and cathelicidins, have diversified over time. In this review, we describe...
Suppl. data (body text: https://doi.org/10.1016/j.rvsc.2021.08.013)
Coccidiosis is a disease caused by Eimeria, which represents the first parasitic disease in poultry farming. Among them, E. tenella is a virulent species which specifically colonizes the caecum. The inflammatory response to infection is associated to numerous host proteases including cysteine cathepsins that can be deleterious for tissue and innate...
Defensins are natural antimicrobial peptides. The avian beta-defensin AvBD7 isolated from the chicken bone marrow possess broad antibacterial spectrum and strong resistance to proteolysis. However, its ability to fight systemic infections of major concern for public health, such as salmonellosis, is unknown. As a first approach, fluorescence labeli...
Vasoactive peptides are key early mediators of inflammation released through activation of different enzymatic systems. The mammalian kinin-kallikrein (K-KLK) system produces bradykinin (BK) through proteolytic cleavage of a kininogen precursor by enzymes named kallikreins. BK acts through specific ubiquitous G-protein coupled receptors (B1R and B2...
We report here the complete genome sequences of two Myoviridae phages that infect various avian-pathogenic Escherichia coli strains and that are closely related to phage phAPEC8.
Les principes, contraintes et questions de recherche relatifs à plusieurs perspectives d’alternatives thérapeutiques aux antibiotiques sont présentés. Différentes approches telles que la phytothérapie et la phagothérapie, basée sur l’activité lytique des phages infectant les bactéries, sont anciennes mais leur utilisation pratique nécessiterait des...
Defensins are frontline peptides of mucosal immunity in the animal kingdom, including birds. Their resistance to proteolysis and their ensuing ability to maintain antimicrobial potential remains questionable and was therefore investigated. We have shown by bottom-up mass spectrometry analysis of protein extracts that both avian beta-defensins AvBD2...
AvBD2 and AvBD7 are resistant to proteolysis by trypsin, chymotrypsin, neutrophil elastase and cathepsin D.
(A) AvBD2 (12.5 μM) was incubated in the presence of trypsin, chymotrypsin, neutrophil elastase or cathepsin D during 4 h at 30°C (substrate-enzyme ratio: 100) as described in detail in the experimental section. (B) The same procedure was rep...
Identification of proteins from extracts of intestinal segments.
Results are given by computational analysis of bottom-up mass spectrometry (nanoLC-MS/MS) data. For each identified protein, NCBInr accession number is given. Proteins of interest (defensins and cathepsins) are indicated in red.
(XLSX)
Structural characterization of avian β-defensins 1, 2 and 7 petidoforms by top-down high resolution mass spectrometry (HR-MS).
Interpreted nanoESI HR-MS and HR-MS/MS spectra are presented. In each HCD fragmentation spectrum obtained for (A) AvBD2 and (B) AvBD7 and AvBD1, a zoom of the multicharged precursor is presented with the annotated fragmenta...
Poster - Abstract. Prix du meilleur poster par le GDR-CNRS MUltiFOnctions des Peptides AntiMicrobiens
Lung cysteine cathepsins B, K, L and S contribute to physiological and pathological processes including degradation of antimicrobial peptides/proteins (AMPs) such as surfactant protein SP-A, lactoferrin, secretory leukocyte peptidase inhibitor and beta-defensins -2 and -3. Substantial amounts of uncleaved LL-37, a 37-mer cationic AMP, were observed...
Avian influenza viruses are circulating continuously in ducks, inducing a mostly asymptomatic infection, while chickens are accidental hosts highly susceptible to respiratory disease. This discrepancy might be due to a different host response to the virus between these two bird species and in particular to a different susceptibility to reinfection....
Avian influenza viruses are circulating continuously in ducks, inducing a mostly asymptomatic infection, while chickens are accidental hosts highly susceptible to respiratory disease. This discrepancy might be due to a different host response to the virus between these two bird species and in particular to a different susceptibility to reinfection....
Poster - Abstract
Increasing feed efficiency of broiler chickens by selective breeding could lead to decreased feed cost and reduced environmental impact of poultry production. At INRA, two broiler chicken lines (D + /D - ) were divergently selected for their digestive efficiency. Strong differences were shown between both lines for the anatomy and histology of the...
Oral communication - Abstract in Proceedings
Poultry production will have to be increased in the future to meet the demands of the growing human population. This will increase the pressure on land use to produce cereals and proteins used in poultry diets and also the environmental pressure due to the increase of manure produced by these birds. Selecting birds able to digest more varied and le...
Colloque « Pour des aliments sains : savoir maîtriser les risques en alimentation » à Toulouse le 28 novembre 2012.
Numerous β-defensins have been identified in birds, and the potential use of these peptides as alternatives to antibiotics has been proposed, in particular to fight antibiotic-resistant and zoonotic bacterial species. Little is known about the mechanism of antibacterial activity of avian β-defensins, and this study was carried out to obtain initial...
There is increasing evidence that activation of innate immunity, in animals and man, by live vaccines, sub-unit vaccines or synthetic or non-synthetic stimulants can induce a profound and rapidly induced resistance to pathogens, including infectious agents that are unrelated to the stimulating antigen or agent. We review the evidence for this pheno...
Natural antimicrobial peptides are present in different compartments (eggshell, egg white, and vitelline membranes) of the
hen egg and are expected to be involved in the protection of the embryo during its development and to contribute to the production
of pathogen-free eggs. In the present study, we used vitelline membranes from hen (Gallus gallus...
beta-Defensins are important components of innate immunity in mucosal tissue, a major entry site for several pathogens. These small cationic peptides possess antimicrobial activity against various microorganisms including Salmonella. Two chicken inbred lines, 6 and 15I, diverge phenotypically with respect to levels of Salmonella Enteritidis intesti...
Three biologically active β-defensins were purified by chromatography from chicken bone marrow extract: avian β-defensin 1
(AvBD1), AvBD2, and the newly isolated β-defensin AvBD7. Mass spectrometry analyses showed that bone marrow-derived AvBD1,
-2, and -7 peptides were present as mature peptides and revealed posttranslational modifications for AvB...
La majorité des cas de salmonellose humaine dans les pays industrialisés est liée à la consommation d’oeufs et de viande de volaille mais aussi de charcuteries contaminées. Pour réduire ce risque alimentaire, la stratégie développée à l’INRA, en collaboration avec l’AFSSA, vise principalement à réduire la contamination des matières premières alimen...
Increasing resistance to acute Salmonellosis (that is, contamination level shortly after infection) is not sufficient to reduce the risk for consumers to be contaminated by Salmonella. Indeed, animals may remain contaminated at a low level for weeks or months. Increased resistance to the Salmonella carrier state, i.e., animals' ability to clear bac...
Most human cases of salmonellosis in developed countries are related to the consumption of contaminated eggs, poultry and pig meat. To improve food safety, INRA aims, in collaboration with AFSSA, at decreasing the prevalence of contamination of raw animal material, especially those originating from poultry or pigs. This implies improving animal hea...
Increasing resistance to acute Salmonellosis (that is, contamination level shortly after infection) is not sufficient to reduce the risk for consumers to be contaminated by Salmonella. Indeed, animals may remain contaminated at a low level for weeks or months. Increased resistance to the Salmonella carrier state, i.e. animals’ ability to clear bact...
Salmonellosis is one of the main causes of food-borne poisoning due to the consumption of contaminated poultry products. In the flocks, Salmonella is able to persist in the digestive tract of birds for weeks without triggering any symptom. In order to identify molecules and genes involved in the mechanism of host resistance to intestinal carrier-st...
Asymptomatic Salmonella enterica serovar Enteritidis carrier state in poultry has serious consequences on food safety and public health due to the risks of food poisoning following consumption of contaminated products. An understanding the mechanisms of persistence of Salmonella in the digestive tract of chicken can be achieved by a better knowledg...
Interleukins (ILs) are known best for their involvement in the immune system and their role during inflammation. In the ovary, a growing body of evidence suggests that the ovarian follicle is a site of inflammatory reactions. Thus ovarian cells could represent sources and targets of ILs. Since then, the IL-1 system components (IL-1alpha, IL-1beta,...
A growing body of evidence suggests that the ovary is a site of inflammatory reactions, and thus, ovarian cells could represent sources and targets of the interleukin-1 (IL-1) system. The aim of the present work was to investigate the expression of IL-1alpha, IL-1beta, IL-1ra, IL-1R1, and IL-1R2 genes in equine cumulus cells and oocytes. Moreover,...
In order to investigate the determinism of the host specificity and to better understand the host resistance mechanisms, infections of sheep were performed with either S. abortusovis, serotype specific for ovine species, or with S. dublin, serotype adapted to cattle and accidentally transmissible to human. Following a subcutaneous challenge, S. dub...
The early interferon-gamma (IFN-gamma) response after Salmonella infection was compared in resistant and susceptible congenic mice strains differing by the allele of Nramp1. IFN-gamma gene expression in vivo was studied by quantitative reverse transcription-PCR performed from the spleen of mice challenged by Salmonella either in multiplication or i...
A quantitative competitive RT-PCR method was developed in order to measure IL-1beta, IL-4, IL-12, IFNgamma, TNFalpha and G(3)PDH mRNA from samples of ovine tissue such as lymph node or spleen. The main advantage of the method relies on the use, for each target sequence, of an internal competitor construct similar to the relevant target, but 4-bp di...
Knowledge of the host response, of the resistance process, and of the mediators committed against Salmonella infection is essential to progress towards better means of prophylaxis and eradication. In this context, the present contribution attempts to interconnect, with the pivotal role of the macrophage, the early resistance process under the contr...
*INRA Pathologie infectieuse et immunologie 37380 Nouzilly (FRA) Diffusion du document : INRA Pathologie infectieuse et immunologie 37380 Nouzilly (FRA)
The aim of this work was to define the respective responsibilities of the lipid and protein components of syncytiotrophoblast plasma membranes on the inhibition of lymphocyte proliferation induced in vitro.
A fractionation method using octyl-beta-D-glucopyranoside enabled lipoprotein, lipid, and protein fractions to be isolated from the membrane.
T...
In comparison to B cell stimulation mediated by surface immunoglobulin (Ig) antigen receptor ligation, little is known about the intracellular events associated with T cell-dependent B cell responses. A model for the efferent phase of T cell-B cell interaction was used to examine the capacity of activated T cells to trigger nuclear expression of th...
The ability of syncytiotrophoblast plasma membrane lipid and protein fractions (STPM lipids, STPM proteins), tested under a reconstituted form, to inhibit lymphocyte proliferation induced by PHA was investigated. The cytostatic activity of STPM proteins appeared greater than that of the STPM lipids. Furthermore, IL-2 production and IL-2 receptor ex...
The effects of syncytiotrophoblast plasma membrane vesicles (STPM) on stimulated Jurkat leukemic T cells have been investigated. STPM inhibited IL-2 production and the expression of protein P55 of the IL-2 receptor (IL-2R P55), when Jurkat cells were stimulated by a combination of calcium ionophore A23187 (CaI) + phorbol 12-myristate 13-acetate (PM...
The mechanisms by which vesicles of syncytiotrophoblast plasma membranes (STPM) prepared from full-term human placentas inhibit lymphocyte proliferation have been investigated. In the presence of STPM, IL-2 secretion and the expression of protein P55 (IL-2R P55) from its receptor were examined in two models of PBMC proliferation: induced by PHA in...
We have used an ELISA procedure to compare the reactivity of samples with or without IgG anticardiolipin antibodies (ACA) on cardiolipin-coated wells (target wells) and cardiolipin-free wells (control wells), using 10% fetal calf serum (10% FCS), 10% adult bovine serum (10% ABS) or 1% bovine serum albumin (1% BSA) as buffer. With 1% BSA, ACA reacti...
