Anne Albrecht

Anne Albrecht
Otto-von-Guericke-Universität Magdeburg | OvGU · Institut für Anatomie

Professor

About

43
Publications
8,512
Reads
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457
Citations
Introduction
We are interested on how such stress across the life span shape local GABAergic interneuron circuits, signaling by modulatory neuropeptides as well as astrocyte-neuron-interactions in the dorsal and ventral hippocampus and its interaction with other structures of the limbic system. We combine biochemical and molecular expression analysis, pharmacological and viral interventions as well as behavioral tests to investigate neuronal mechanisms of stress (mal-)adaptation and learning.
Additional affiliations
October 2016 - present
Leibniz Institute for Neurobiology
Position
  • PostDoc Position
October 2012 - September 2016
University of Haifa
Position
  • Haifa University
December 2008 - September 2012
Otto-von-Guericke-Universität Magdeburg
Education
December 2008
October 2001 - November 2008
Otto-von-Guericke-Universität Magdeburg
Field of study
  • Human Medicine

Publications

Publications (43)
Preprint
Remembering the location of food is essential for survival. Rodents and humans employ mainly hippocampus-dependent spatial strategies, but when being stressed they shift to striatum-mediated stimulus-based strategies. To investigate underlying brain circuits, we tested mice with a heightened stress susceptibility due to a lack of the GABA-synthetiz...
Article
We recently introduced behavioral profiling as a translational approach to increase the validity of animal models of posttraumatic stress disorder (PTSD). Behavioral profiling utilizes the response of a ‘normal population’ of control animals and compares the performance of animals with a history of traumatic stress in different behavioral tests tha...
Article
Full-text available
Early life stress is an important vulnerability factor for the development of anxiety disorders, depression and late‐onset cognitive decline. Recently we demonstrated that juvenile stress (JS) lastingly enhanced long‐term potentiation via reduction of steady‐state glutamine synthetase mRNA expression and the associated dysregulation of the astrocyt...
Article
Studies in humans and rodents suggest a critical role for the hippocampal formation in cognition and emotion, but also in the adaptation to stressful events. Successful stress adaptation promotes resilience, while its failure may lead to stress-induced psychopathologies such as depression and anxiety disorders. Hippocampal architecture and physiolo...
Article
Full-text available
Adverse experiences during childhood are among the most prominent risk factors for developing mood and anxiety disorders later in life. Early-life stress interventions have been established as suitable models to study the neurobiological basis of childhood adversity in rodents. Different models such as maternal separation, impaired maternal care an...
Article
Full-text available
Only a minority of trauma-exposed individuals develops Posttraumatic stress disorder (PTSD) and active processes may support trauma resilience. Individual behavioral profiling allows investigating neurobiological alterations related to resilience or pathology in animal models of PTSD and is utilized here to examine the activation of different inter...
Article
A traumatic childhood is among the most important risk factors for developing stress-related psychopathologies such as posttraumatic stress disorder or depression later in life. However, despite the proven role of astrocytes in regulating transmitter release and synaptic plasticity, the contribution of astrocytic transmitter metabolism to such stre...
Article
Full-text available
Activation of the amygdala is one of the hallmarks of acute stress reactions and a central element of the negative impact of stress on hippocampus-dependent memory and cognition. Stress-induced psychopathologies, such as posttraumatic stress disorder, exhibit a sustained hyperactivity of the amygdala, triggered at least in part by deficits in GABAe...
Article
Full-text available
Cholinergic neuromodulation in the hippocampus controls the salience of background context memory acquired in the presence of elemental stimuli predicting an aversive reinforcement. With pharmacogenetic inhibition we here demonstrate that hilar perforant path-associated (HIPP) cells of the dentate gyrus mediate the devaluation of background context...
Article
Full-text available
The formation of fear memories is a powerful and highly evolutionary conserved mechanism that serves the behavioral adaptation to environmental threats. Accordingly, classical fear conditioning paradigms have been employed to investigate fundamental molecular processes of memory formation. Evidence suggests that a circadian regulation mechanism all...
Article
ALBRECHT, A., MÜLLER, I., ARDI, Z., ÇALIŞKAN, G., GRUBER, D., IVENS, S., SEGAL, M., BEHR, J., HEINEMANN, U., STORK, O., and RICHTER-LEVIN, G. Neurobiological consequences of juvenile stress: a GABAergic perspective on risk and resilience. NEUROSCI BIOBEHAV REV XXX-XXX, 2016. − Childhood adversity is among the most potent risk factors for developing...
Article
Full-text available
Inhibitory synaptic transmission in the amygdala plays a pivotal role in fear learning and its extinction. However, the local circuits formed by GABAergic inhibitory interneurons within the amygdala and their detailed function in shaping these behaviors are not well understood. Here, we employed lentiviral-mediated knockdown of the cell adhesion mo...
Article
Childhood trauma is a well-described risk factor for the development of stress-related psychopathology such as posttraumatic stress disorder or depression later in life. Childhood adversity can be modeled in rodents by juvenile stress (JS) protocols, resulting in impaired coping with stressful challenges in adulthood. In the current study, we inves...
Article
Aversive experiences in early life are thought to dispose to psychopathologies such as mood or anxiety disorders. In a two-hit stress model, we assessed the effects of juvenile and/or adult stress on the 5-HT-mediated modulation of synaptic inhibition of ventral dentate gyrus granule cells. Combined but not single stress exposure led to a significa...
Article
Full-text available
The CA3 associative network plays a critical role in the generation of network activity patterns related to emotional state and fear memory. We investigated long-term changes in the corticosterone-sensitive function of this network following fear conditioning and fear memory reactivation. In acute slice preparations from mice trained in either cond...
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Molecular processes in GABAergic local circuit neurons critically contribute to information processing in the hippocampus and to stress-induced activation of the amygdala. In the current study, we determined expression changes in GABA-related factors induced in subregions of the dorsal hippocampus as well as in the BLA of rats 5 h after spatial lea...
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Pharmacological evidence suggests that the neuropeptide somatostatin (SST) exerts anxiolytic action via the amygdala, but findings concerning the putative role of endogenous SST in the regulation of emotional responses are contradictory. We hypothesized that an endogenous regulation of SST expression over the course of the day may determine its fun...
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Fearful associations can be replaced by neutral associations through repetitive exposure of an individual to the fearful situation without the aversive component. Recently, Peña and his colleagues demonstrated that pairing activation of noradrenergic (NA) pathways through vagus nerve stimulation (VNS) with extinction learning accelerates consolidat...
Article
Full-text available
Evidence suggests that regulated ubiquitination of proteins plays a critical role in the development and plasticity of the central nervous system. We have previously identified the ubiquitin ligase Praja1 as a gene product induced during fear memory consolidation. However, the neuronal function of this enzyme still needs to be clarified. Here, we i...
Data
Confirmation of a neuronal phenotype. PC12 cells of each stably transfected line were stained with antibodies against the neuron-specific markers beta3-tubulin (also called TuJ1; red) or MAP2 (red), each in combination with DAPI (cyan) and anti-alpha-tubulin (blue). (A) through (C) show the separate and merged stainings for cells overexpressing Pra...
Data
Praja1-induced polyubiquitination and changes of NRAGE levels in COS-7 cells. Immunoblot analysis shows an increase of total protein ubiquitination and reduction of endogenous NRAGE in cells overexpressing HA-tagged Praja1.1 and Praja1.2 (lane 2) or HA-tagged Praja1.2 alone (lane 3) compared to mock controls (lane 1). At the same time, levels of NR...
Data
Substrate independence of Praja effects. (A) Reduced growth of neurites of ≥25 µm is observed in cells overexpressing Praja1.1/2, regardless of the substrate used. (B) Cell rounding is reduced on all tested substrates except for collagen IV. (C) However, the overall proportion of differentiated cells, showing a neuron-like morphology, is generally...
Data
Specificity of anti-Praja1 serum. The expression of both Praja1.1 and Praja1.2 from HA-tagged praja1.1 (lane 3) is detected in HEK293T cells using Praja-specific serum. The apparent molecular weight of ca. 95 kD and 65 kD, respectively, differs clearly from the predicted molecular weight of the two isoforms but is in agreement with the previously r...
Data
Induction of apoptosis in COS-7 cells through Praja1 expression. (A) COS-7 cells acutely transfected with praja1.1 or praja1.2 display cell rounding and microspike formation (arrow), and develop pyknotic nuclei, indicating an induction of apoptosis. EGFP and HA fusion proteins are equally effective in inducing this phenotype (data not shown), where...
Data
Supplemental experimental procedures. (DOC)
Article
Full-text available
Pathological fear and anxiety can be studied, in rodents, with fear conditioning and exposure to reminder cues. These paradigms are thought to critically involve the ventral hippocampus, which also serves as key site of glucocorticoid action in the brain. Here, we demonstrate a long-lasting reduction of kainate-induced gamma oscillations in slice p...
Article
Full-text available
Genetic and biomarker studies in patients have identified the Neural Cell Adhesion Molecule (NCAM) and its associated polysialic acid (PSA) as a susceptibility factors for schizophrenia. NCAM and polysialtransferase mutant mice have been generated that may serve as animal models for this disorder and allow to investigate underlying neurodevelopment...
Article
Full-text available
Evidence suggests that the neural cell adhesion molecule (NCAM) is an important molecular constituent of adaptive and maladaptive circuit (re-)organization in the central nervous system. Here, we further investigate its putative involvement in amygdala and hippocampus functions during context fear memory formation. Using laser capture microdissecti...
Article
Experimental animal models provide an important tool for the identification of inheritable components of fear and anxiety. 'Pavlovian' fear conditioning has been tremendously successful to characterize the neuronal circuitry and cellular mechanisms of the formation, consolidation and extinction of fear memories. Here we summarize recent progress th...

Questions

Question (1)
Question
Can anyone recommend an anti-HA antibody in tissue that works well in tissue (host specifies preferably not rabbit)?
We have mouse brains injected with a lentiviral construct that expresses an HA-tag. We would like to visualise the HA-tag in mouse brain sections (usually fixed with 4% PFA, 30 micrometer thick) via fluorescence immunhistochemistry and double label for other markers.
Thanks a lot!

Projects

Projects (3)
Project
Here, we aim to identify electrophysiological biomarkers for pathological fear and anxiety through investigation of hippocampal network oscillations and its interactions with other limbic oscillations using variety of behavioural tests and rodent models of impaired fear extinction and exessive anxiety.