Annalisa Bianchera

Annalisa Bianchera
Università di Parma | UNIPR · Department of Pharmacy- University of Parma

PhD

About

37
Publications
8,652
Reads
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529
Citations
Additional affiliations
August 2011 - present
Biopharmanet-TEC
Position
  • Research Associate
Description
  • Formulation development, controlled drug release, chitosan scaffolds for regenerative medicine
June 2008 - December 2008
Università di Parma
Position
  • Scholarship
Description
  • Use of stem cells in regenerative medicine
Education
January 2008 - December 2011
Università di Parma
Field of study
  • Molecular Biology and Pathology
October 2004 - April 2007
Università di Parma
Field of study
  • Biotechnology for Health
October 2001 - July 2004
Università di Parma
Field of study
  • Biotechnology

Publications

Publications (37)
Article
Objectives Evidence shows that bioactive (poly)phenols are pivotal in the prevention of chronic diseases, in particular in cardiometabolic health. However, the inter-individual variability existing in their bioavailability and physiological response can impact their true efficacy. Individuals showing similar metabolic profiles for specific (poly)ph...
Article
The combination of a chitosan 3D-printed scaffold with a hydrogel matrix containing an elastin-like polypeptide functionalized with the epidermal growth factor (HEGF) was evaluated as a possible strategy to obtain a bioactive platform with stimuli-responsive properties. We designed a chitosan/HEGF hybrid scaffold and examined the physico-chemical p...
Article
Full-text available
The delivery of a dexamethasone formulation directly into the lung appears as an appropriate strategy to strengthen the systemic administration, reducing the dosage in the treatment of lung severe inflammations. For this purpose, a hyaluronic acid-dexamethasone formulation was developed, affording an inhalable reconstituted nanosuspension suitable...
Article
Full-text available
Active dressings acting on multiple fronts are requested in the field of care for chronic skin ulcers in order to ameliorate patient compliance and tissue restoration. Currently, three-dimensional polymeric hydrogels are widely investigated; however, no prototypes aiming to control oxidative stress and bacterial proliferation in the wound bed have...
Article
Full-text available
The search for best performing carriers for dry powder inhalers is getting a great deal of interest to overcome the limitations posed by lactose. The aerosolization of adhesive mixtures between a carrier and a micronized drug is strongly influenced by the carrier solid-state properties. This work aimed at crystallizing kinetically stable D-mannitol...
Article
Full-text available
Three-dimensional (3D) printing has gained popularity in tissue engineering and in the field of cartilage regeneration. This is due to its potential to generate scaffolds with spatial variation of cell distribution or mechanical properties, built with a variety of materials that can mimic complex tissue architecture. In the present study, horse art...
Article
Alpha 1-antitrypsin is one of the first protein therapeutics introduced on the market – more than 30 years ago – and, to date, it is indicated only for the treatment of the severe forms of a genetic condition known as alpha-1 antitrypsin deficiency. The only approved preparations are derived from plasma, posing potential problems associated with it...
Article
Full-text available
Nanotherapeutics based on biocompatible lipid matrices allow for enhanced solubility of poorly soluble compounds in the treatment of ophthalmic diseases, overcoming the anatomical and physiological barriers present in the eye, which, despite the ease of access, remains strongly protected. Micro-/nanoemulsions, solid lipid nanoparticles (SLN) or nan...
Article
Full-text available
Ucuùba fat was characterized and used as the lipid phase for the production nanostructured lipid carriers (NLCs). The acid (38.76 mg KOH·g‐1), iodine (8.5 g I2·100 g‐1), refraction (1.45), saponification (230.21 mg KOH·g‐1) values as well as oxidative stability (>20h) of the fat were determined. The myristic acid was found to be the main fatty acid...
Article
Introduction Microbial resistance is a severe problem for clinical practice due to misuse of antibiotics that promotes the development of surviving strategies by bacteria and fungi. Microbial cells surrounded by a self-produced polymer matrix, defined as biofilms, are inherently more difficult to eradicate. Biofilms endow bacteria with a unique res...
Article
Introduction The oral route still represents the most popular way of administration of drugs; nowadays oral administration faces new challenges, in particular with regards to the delivery of APIs that are poorly absorbed and sensitive to degradation such as macromolecules and biotechnological drugs. Nanoparticles are promising tools for the efficie...
Chapter
The wide choice of biomaterials available, with their distinct physicochemical features, together with new technologies, is the key to facing the challenge of customization, offering tailored solutions for the variety of situations requiring tissue regeneration, as in the case of wounds. Biomaterials for wound healing can be classified in different...
Article
Full-text available
A decisive step in cell-biomaterial interaction is represented by the adsorption of proteins at the interface, whose fine control may be useful to trigger proper cell response. To this purpose, we can selectively control protein adsorption on biomaterials by means of aptamers. Aptamers selected to recognize fibronectin dramatically enhance chitosan...
Preprint
Full-text available
Drug delivery to the brain represents a challenge especially in the therapy of central nervous system malignancies. Simvastatin (SVT), as other statins, has shown potential anticancer properties that are difficult to exploit in the CNS. In the present work the physico-chemical, mucoadhesive and permeability enhancing properties of simvastatin-loade...
Article
Full-text available
3D biomaterial manufacturing strategies show an extraordinary driving force for the development of innovative therapies in the tissue engineering field. Here, the behaviour of 3D printed chitosan (CH)-based scaffolds was explored as a function of the post-printing gelation process. To this purpose, gel forming properties of different media were tes...
Article
Rationale Protein dynamics are fundamental for biological activity. Direct surface functionalization with these biomolecules often creates regions presenting non‐homogeneous and altered protein functionality. Aptamers have been shown to be an effective method to enhance the biocompatibility of biomaterials by acting as docking points for biomolecul...
Article
Here we have developed and validated an original LC-MS/MS SRM procedure flexible enough to quantitatively screen collagen types I-V in copies of the same type of stromal matrix prepared with different protocols of cell removal to retain the native 3D architecture of the ECM. In a first step, identification of tryptic sequences exclusive to specific...
Article
The fabrication of porous 3D printed chitosan (CH) scaffolds for skin tissue regeneration and their behavior in terms of biocompatibility, cytocompatibility and toxicity toward human fibroblasts (Nhdf) and keratinocytes (HaCaT), are presented and discussed. 3D cell cultures achieved after 20 and 35 days of incubation showed significant in vitro qua...
Article
Despite the increasing number of effective therapeutics for eye diseases, their treatment is still challenging due to the presence of effective barriers protecting eye tissues. Cell Penetrating Peptides (CPPs), synthetic and natural short amino acid sequences able to cross cellular membrane thanks to a transduction domain, have been proposed as pos...
Article
Full-text available
The augmented demand for medical devices devoted to tissue regeneration and possessing a controlled micro-architecture means there is a need for industrial scale-up in the production of hydrogels. A new 3D printing technique was applied to the automation of a freeze-gelation method for the preparation of chitosan scaffolds with controlled porosity....
Article
Full-text available
The aim of the present study was to investigate how the enrichment of chitosan films with anti-fibronectin aptamers could enhance scaffold colonization by osteoblasts, by improving their adhesion and accelerating their proliferation. Chitosan discs were enriched with excess of anti-fibronectin aptamer. Aptamer adsorption on chitosan was monitored b...
Article
The present study investigated the biocompatibility of chitosan films and scaffolds modified with d-(+)raffinose and their capability to support the growth and maintenance of the differentiation of articular chondrocytes in vitro. Primary equine articular chondrocytes were cultured on films and scaffolds of modified d-(+) raffinose chitosan. Their...
Article
There are no reports of saccharolytic enzymes being used in the preparation of formulations for animal semen extenders. In the present study, the authors evaluated the performance of an innovative semen extender (Formula12®) in the long-term liquid storage of boar semen at 17 °C. The formulation used a disaccharide (sucrose) as the energy source pr...
Article
Introduction: The main target of tissue engineering is the preparation and application of adequate materials for the design and production of scaffolds, that possess properties promoting cell adhesion, proliferation and differentiation. The use of natural polysaccharides, such as chitosan, to prepare hydrogels for wound healing and controlled drug...
Chapter
Protein adsorption on surfaces occurs shortly after scaffold insertion. This process is of pivotal importance to achieve therapeutic success in tissue engineering (TE), and favorable proteins should be adsorbed at the interface without unfolding to preserve their structure and function. Protein misfolding at the interface is a common phenomenon, wh...
Article
Full-text available
This study aimed to investigate the preservability and viability of the rabbit spermatozoa diluted in a new semen extender Formula® in comparison with Lepus® at 17 °C of storage. The main characteristic of the new extender formulation is the use of an enzymatic agent associated to a polysaccharide as energy source precursor, added with gentamycin....
Article
Rationale: Drug development efforts involving therapeutic peptides or proteins strongly lead optimization of drug delivery, drug stability, solubility and functionality. Key feature of controlled drug delivery is the use of biocompatible polymers able to interact via non-covalent bonds with an active principle through multiple functional groups. H...
Conference Paper
Full-text available
No disclosure exists wherein the saccharolytic enzymes are associated to the preparation of formulations for animal semen extenders (International Publication Number WO 2015/193265 A1). In the present study, the Authors developed an innovative boar semen extender (Formula®) for long–term storage at 15°C. The extender formulation is based on the use...
Article
The potential of 3D printing technology was here exploited to prepare tailored polylactic acid (PLA) supports for desorption electrospray ionization (DESI) experiments. PLA rough solid supports presenting wells of different shape (i.e. cylindrical, cubic and hemispherical cavities) were designed to accommodate samples of different physical state. T...
Article
Full-text available
The aim of the present study was to investigate whether chitosan-based scaffolds modified with D-(+) raffinose and enriched with thiol-modified gelatin could selectively improve osteoblast adhesion and proliferation. 2, 3 and 4.5% chitosan films were prepared. Chitosan suitability for tissue engineering was confirmed by protein adsorption assay. Sc...
Article
Chitosan is a biodegradable, biocompatible polymer of natural origin widely applied to the preparation of functional hydrogels suitable for controlled release of drugs, peptides and proteins. Non-covalent interactions, expecially ionic interactions, are the main driver of the loading and release behaviour of amino acids or peptides from chitosan hy...
Article
Full-text available
A novel, simple and reliable reversed-phase liquid chromatography (LC)–spectrophotometric UV stability-indicating method was developed and validated for the simultaneous assay of marbofloxacin, clotrimazole and dexamethasone acetate in the presence of their impurities and degradation products in a pharmaceutical formulation for veterinary use. A C1...
Article
Full-text available
This paper provides an analytical characterization of chitosan scaffolds obtained by freeze-gelation toward the uptake and the controlled release of chondroitin sulphate (CS), as cartilage repair agent, under different pH conditions. Scanning electron microscopy (SEM), attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR),...
Article
Full-text available
Two simple and fast C18 and HILIC liquid chromatography-electrospray mass spectrometry methods for the determination of hyaluronic acid (HA) in a mucoadhesive chitosan-based formulation were developed and validated. The performances of both methods were compared in terms of validation parameters and matrix effect. A simple sample preparation method...
Article
Full-text available
The translocation t(9;11)(p22;q23) generates the MLL-AF9 oncogene and is commonly associated with monocytic acute myeloid leukemia (AML-M5; FAB-classification). For the oncogenicity of MLL-AF9, the (over)expression of several other genes, including selected HOXA cluster genes as well as MEIS1 (a HOX cofactor), is required. We previously showed that...

Projects

Projects (2)
Project
The objective of this project is to provide new tools for the therapy of Localized Bone Defects (LBDs) through the pharmaceutical and preclinical development of new medical devices constituted by a combination of a bioresorbable scaffold and an already approved drug with osteo/chondrogenic activity
Project
Original 3D printing technology for 3D printing of medicated hydrogels supporting in situ one-stepapplication of biocompatible and bioresorbable healing patches. • Patented chitosan hydrogels • Improved porous structure and cell adhesion • No organic solvents or chemical modifications needed • Suitable for loading of active molecules and controlled drug release • Micro geometry optimized to promote cell growth • Macro geometry customizable to fit ulcers • Breathable structure • No expensive or toxic additives