Anna Kisiala

Anna Kisiala
Trent University · Department of Biology

Ph.D. (Agriculture)

About

67
Publications
30,994
Reads
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985
Citations
Citations since 2017
36 Research Items
835 Citations
2017201820192020202120222023050100150200
2017201820192020202120222023050100150200
2017201820192020202120222023050100150200
2017201820192020202120222023050100150200
Additional affiliations
November 2011 - present
Trent University
Position
  • PostDoc Position
November 2010 - October 2011
Nipissing University
Position
  • PostDoc Position
September 2006 - December 2018
University of Technology and Life Sciences in Bydgoszcz
Position
  • Research Associate
Education
October 2002 - July 2008
October 2000 - June 2002
University of Technology and Life Sciences in Bydgoszcz
Field of study
  • Post-Graduate Teachers College
October 1997 - July 2002

Publications

Publications (67)
Article
Full-text available
Bacteria present in the rhizosphere of plants often synthesize phytohormones, and these signals can consequently affect root system development. In legumes, plants adapt to nitrogen starvation by forming lateral roots as well as a new organ, the root nodule, following a symbiotic interaction with bacteria collectively referred to as rhizobia. As cy...
Article
Full-text available
Phytohormones regulate multiple processes of plant physiology, including responses to biotic and abiotic stresses. Among phytohormones, cytokinins (CKs) are an important group of growth regulators responsible for cell division, nutrient allocation and photosynthetic performance. In this work, the effect of an increased pool of CKs in lentil (Lens c...
Article
Cytokinins (CKs) are adenine derivatives that act as phytohormones. These signaling molecules control plant cell division and differentiation, organ growth and senescence, and orchestrate plant interactions with biotic and abiotic environments. While CKs are predominately recognized as plant-based substances, CKs have been found across different do...
Article
Full-text available
Cytokinins (CKs) are a group of adenine-derived, small signaling molecules of crucial importance for growth and multiple developmental processes in plants. Biological roles of classical CKs: isopentenyladenine (iP), trans and cis isomers of zeatin (tZ, cZ), and dihydrozeatin, have been studied extensively and their functions are well defined in man...
Article
Full-text available
Background Symbiotic Methylobacterium strains comprise a significant part of plant microbiomes. Their presence enhances plant productivity and stress resistance, prompting classification of these strains as plant growth-promoting bacteria (PGPB). Methylobacteria can synthesize unusually high levels of plant hormones, called cytokinins (CKs), includ...
Article
Full-text available
Clubroot, a devastating soil-borne root disease, in Brassicaceae is caused by Plasmodiophora brassicae Woronin (P. brassicae W.), an obligate biotrophic protist. Plant growth and development, as well as seed yield of Brassica crops, are severely affected due to this disease. Several reports described the molecular responses of B. napus to P. brassi...
Preprint
Full-text available
Isopentenyltransferases ( IPT s), including adenosine phosphate-isopentenyltransferases ( ATP/ADP-IPT s and AMP-IPT s) and tRNA‐isopentenyltransferases ( tRNA-IPT s), are responsible for a rate-limiting step of cytokinin (CK) biosynthesis. tRNA-IPT s, which account for the synthesis of cis -zeatin ( c Z)-type CKs, are less understood and often thou...
Article
Fatty acid (FA) levels and profiles are vital for soybean oil quality, while cytokinins (CKs) and abscisic acid (ABA) are potent regulators of plant growth and development. Previous research suggested associations between FA biosynthesis and hormonal signalling networks; however, hormonal regulation of FA accumulation during soybean ( Glycine max )...
Preprint
Full-text available
Background Symbiotic Methylobacterium strains comprise a significant part of plant microbiomes. Their presence enhances plant productivity and stress resistance, prompting classification of these strains as plant growth-promoting bacteria (PGPB). Methylobacteria can synthesize unusually high levels of plant hormones, called cytokinins (CKs), includ...
Article
Full-text available
Main conclusion Riboside type cytokinins are key components in cytokinin metabolism, transport, and sensitivity, making them important functional signals in plant growth and development and environmental stress responses. Abstract Cytokinin (CKs) are phytohormones that regulate multiple processes in plants and are critical for agronomy, as they ar...
Article
Full-text available
Trichoderma is an important genus of symbiotic fungi, commonly used around the world as biocontrol agents and as biofertilizer. Although their beneficial effects are well known and are successfully exploited in sustainable agriculture practices, the biochemical mechanisms of plant growth-promoting actions of Trichoderma and their anti-pathogen char...
Article
Full-text available
Isopentenyltransferase (IPT) in plants regulates a rate-limiting step of cytokinin (CTK) biosynthesis. IPTs are recognized as key regulators of CTK homeostasis and phytohormone crosstalk in both biotic and abiotic stress responses. Recent research has revealed the regulatory function of IPTs in gene expression and metabolite profiles including sour...
Article
Full-text available
The number of organisms that are known to produce cytokinins (CKs) continues to increase. In fact, species from all life kingdoms have now been shown to either produce CKs or at least have the genetic components to make it possible. In vitro growth of microorganisms, plant/animal cells, and tissue cultures often requires nutrient-rich media compose...
Article
Previous observations of Eriophorum vaginatum L. growing in its natural habitats suggest that this perennial graminoid is highly tolerant of aluminum (Al). Eriophorum vaginatum is unusually productive in the bogs surrounding Sudbury (Ontario, Canada) where many years of intensive mining and industrial activities resulted in strong acidification of...
Article
Full-text available
Cytokinins (CKs) play a fundamental role in regulating dynamics of organ source/sink relationships during plant development, including flowering and seed formation stages. As a result, CKs are key drivers of seed yield. The cytokinin oxidase/dehydrogenase (CKX) is one of the critical enzymes responsible for regulating plant CK levels by causing the...
Article
Full-text available
The constant spread of heavy metal contamination creates an increasing global environmental issue that results in considerable deterioration of land and water ecosystems leading to a decline in the health of plants, animals and humans. Novel, algal-based filtration technologies have been gaining a great deal of attention given their eco-friendly, e...
Article
Full-text available
Heavy metal (HM) contamination of the environment is a major issue worldwide, creating an ever-increasing demand for remediation techniques. Remediation with algae offers an ecologically safe, cost-effective, and efficient option for HM removal. Similar to plants, growth and development of algae are controlled by the hormonal system, where phytohor...
Article
Full-text available
Heavy metals can represent a threat to the health of aquatic ecosystems. Unlike organic chemicals, heavy metals cannot be eliminated by natural processes such as their degradation into less toxic compounds, and this creates unique challenges for their remediation from soil, water, and air. Phytoremediation, defined as the use of plants for the remo...
Article
Full-text available
Main conclusion: Early cytokinin activity and late abscisic acid dynamics during wheat kernel development correspond to cultivars with higher yield potential. Cytokinins represent prime targets for marker development for wheat breeding programs. Two major phytohormone groups, abscisic acid (ABA) and cytokinins (CKs), are of crucial importance for...
Preprint
Full-text available
Cytokinins (CKs) play a fundamental role in regulating dynamics of organ source/sink relationships during plant development, including flowering and seed formation stages. As a result, CKs are key drivers of seed yield. The cytokinin oxidase/dehydrogenase (CKX) is one of the critical enzymes responsible for regulating plant CK levels by causing the...
Article
Full-text available
Cytokinins (CKs) are adenine-derived, small-molecule plant growth regulators that control aspects of almost all plant growth and development processes. Internally, CKs play significant roles in plant cell division, nutrient allocation, and photosynthetic performance, and they are also detection and signaling agents for plant responses to the enviro...
Article
Full-text available
Cytokinins (CKs) are key phytohormones that not only regulate plant growth and development but also mediate plant tolerance to drought stress. Recent advances in genome-wide association studies coupled with in planta characterization have opened new avenues to investigate the drought-responsive expression of CK metabolic and signaling genes, as wel...
Article
Full-text available
Cytokinins (CKs) are a class of compounds that have long been thought to be exclusively plant growth regulators. Interestingly, some species of phytopathogenic bacteria and fungi have been shown to, and gall-inducing insects have been hypothesized to, produce CKs and use them to manipulate their host plants. We used high performance liquid chromato...
Article
Full-text available
Cytokinins (CKs) are a family of evolutionarily conserved growth regulating hormones. While CKs are well-characterized in plant systems, these N6-substituted adenine derivatives are found in a variety of organisms beyond plants, including bacteria, fungi, mammals, and the social amoeba, Dictyostelium discoideum. Within Dictyostelium, CKs have only...
Article
Full-text available
Main conclusion This study revealed that elevated carbon dioxide increases Arabidopsis tolerance to higher temperature and drought stress by mitigating oxidative stress and improving water status of plants. Abstract Few studies have considered multiple aspects of plant responses to key components of global climate change, including higher temperat...
Article
Full-text available
The arbuscular mycorrhizal (AM) symbiosis between terrestrial plants and AM fungi is regulated by plant hormones. For most of these, a role has been clearly assigned in this mutualistic interaction; however, there are still contradictory reports for cytokinin (CK). Here, pea plants, the wild type (WT) cv. Sparkle and its mutant E151 (Pssym15), were...
Article
Full-text available
Cytokinins (CKs) encompass a group of phytohormones, known to orchestrate many critical processes in plant development. Excluding Archaea, CKs are pervasive among all kingdoms, but much less is reported about their metabolism beyond plants. Recent evidence from mammalian tissues indicates the presence of six additional CK forms beyond the previousl...
Article
Cytokinins (CK) have been extensively studied for their roles in plant development. Recently, they also appeared to ensure crucial functions in the pathogenicity of some bacterial and fungal plant pathogens. Thus, identifying cytokinin-producing pathogens is a prerequisite to gain a better understanding of their role in pathogenicity. Taking advant...
Article
Full-text available
Cytokinins (CKs) are a group of phytohormones essential to plant growth and development. The presence of these N6-modified adenine derivatives has also been documented in other groups of organisms, including bacteria, fungi, and insects. Thus far, however, only a single CK, N6-(Δ2-isopentenyl) adenine-9-riboside (iPR), has been identified in mammal...
Article
While lab and greenhouse based studies have long indicated that cytokinins (CK) promote yield increases in soybean (Glycine max L.), it is not known if the relationship would be valid under more complex field conditions. Thus, an ambitious CK metabolite analysis was undertaken involving long-term field trials of commercial and historical soybean va...
Article
Full-text available
The aim of this work was to examine the function of phytohormones in the pathogenesis of cedar-apple rust, a fungal disease caused by Gymnosporangium juniper-virginianae Schwein. on Eastern red cedar (Juniperus virginiana L.) and crabapple trees (Malus spp. Mill.). Control cedar branchlets, gall tissues, fungal telial horns, as well as healthy and...
Article
Full-text available
In legume plants, low nitrogen soils promote symbiotic interactions with rhizobial bacteria, leading to the formation of nitrogen-fixing root nodules. Amongst critical signals regulating this developmental process are bacterial Nod Factors (NFs) and several plant hormones, including cytokinins (CKs) and gibberellins (GAs). Here we show in Medicago...
Article
Full-text available
Several herbivorous insects and plant-associated microorganisms control the phytohormonal balance, thus enabling them to successfully exploit the plant by inhibiting plant defences and withdrawing plant resources for their own benefit. The leaf-mining moth Phyllonorycter blancardella modifies the cytokinin (CK) profile of mined leaf-tissues, and th...
Article
Full-text available
Nod factors (NF) are lipochitooligosaccharidic signal molecules produced by rhizobia, which play a key role in the rhizobium-legume symbiotic interaction. In this study, we analyzed the gene expression reprogramming induced by purified NF (4h and 24h of treatment) in the root epidermis of the model legume Medicago truncatula. Tissue-specific transc...
Article
Full-text available
Vegetable oil utilization is determined by its fatty acid composition. In soybean and other grain crops, during the seed development oil accumulation is important trait for value in food or industrial applications. Seed development is relatively short and sensitive to unfavorable abiotic conditions. These stresses can lead to a numerous undesirable...
Article
Full-text available
Plants produce cytokinin (CK) hormones for controlling key developmental processes like source/sink distribution, cell division or programmed cell-death. Some plant pathogens have been shown to produce CKs but the function of this mimicry production by non-tumor inducing pathogens, has yet to be established. Here we identify a gene required for CK...
Data
Primers used for RT-qPCR gene expression studies and for PCR genotyping. MoCKS1: Magnaporthe oryzae Cytokinin Synthesis 1; OsRR1, 2, 3, 6, 10 corresponding to the annotation of response regulator coding genes published by Pareek et al., (2006) [53]. PBZ1: Probenazole-inducible gene; CHI and CHI7 coding for chitinases; PR: Pathogenesis Related genes...
Data
The cks1 mutant triggers different host CK signaling and defense transcriptional responses during early stages of infection. (A) The transcriptional regulation of CK marker genes (OsRR2 (Os12g04500), OsRR3 (Os01g72330) and OsRR10 (Os02g35180) as named by Pareek et al., (2006) [53] was evaluated by quantitative RT-PCR using the Actin gene for normal...
Data
The tRNA-IPT protein is highly conserved across Ascomycetes. Phylogenetic tree based on the primary sequence of putative orthologous tRNA-IPT proteins identified by BLASTp in Ascomycetes. Multiple protein sequences alignment was performed with MUSCLE and alignment curation with Gblocks. The phylogenetic tree was obtained using PhyML [100]. The phyl...
Data
The MGG_04857 protein is similar to a tRNA-Isopentenyl transferase. (A) Primary sequence alignment of MOD5, AtIPT2 and the orthologous protein from M. oryzae. MOD5 and AtIPT2 are tRNA-isopentenyl transferases involved in CK biosynthesis in yeast [68] and Arabidopsis [24], respectively. Binding sites (underlined) are conserved in the putative tRNA-I...
Data
Early developmental of cks1 is not modified in vitro or on the plant surface. (A) The mycelial growth of the different strains was initiated from a fungal disc of 1cm of diameter from a first plate where the fungi reached maximum growth. The diameter of mycelia was measured during 13 days on minimal medium. The values are the mean and SD of 5 repli...
Data
The reduced virulence of cks1 strain is restored by exogenous application of cis-zeatin. Plants were treated with 50μM of cis-zeatin or buffer alone 24h after inoculation with cks1 mutant and cks1CKS1 complemented strain. The symptoms were observed 6dpi (A) and the number of lesion per leaf is shown (B). The values represent the mean and SD of thre...
Data
Accumulation of amino acids at the infected site and around. Sugar and amino acid contents were quantified (presented in nmol/mg of fresh weight), during infection (times are indicated), at the site of inoculation corresponding to the”infected zone” and one centimeter apart (respectively named “lower” and “upper non-infected zones”). For more detai...
Data
Putative genes involved in CK metabolisms and signaling pathways, in Oryza sativa, Arabidopsis thaliana, Magnaporthe oryzae and Saccharomyces cerevisiae. CK biosynthesis in rice and Arabidopsis is well described. Based on homology between primary protein sequences some putative orthologous could be identified in M. oryzae and yeast by BLASTp. By th...
Data
Characterization of fungal cks1 mutants and complemented strains. (A) Genomic structure of MoCKS1 (MGG_04857) gene. The cks1 strain was generated by homologous recombination between the endogenous CKS1 gene and a PCR fragment containing the hygromycin resistance gene. The knock-out mutation was complemented by a construct containing the genomic seq...
Data
The cks1 mutant is less tolerant to oxidative stress. The cks1 mutant and complemented strain were grown on minimal medium containing H2O2 and radial growth was measured as a read-out of fungal fitness. (A) The cks1 mutant is hypersensitive to oxidative stress. (*: P<0.001; t-test comparing cks1 and complemented mutant strains of 5 replicates). (B)...
Article
Full-text available
The aim of the research was the agromorphological and molecular analysis of eight DH-R3 lines of pepper (Capsicum annuum L.). The following lines of C. annuum were used in the research – three parental forms: ATZ1, PO, CDT, and R3 generation of DH lines obtained in anther culture of two hybrids (ATZ1×PO)F1 : lines AP1, AP2, AP10, AP15, AP32, AP40 a...
Article
Full-text available
The main objective of this study was to determine the optimal concentrations of a wide spectrum of exogenous phytohormones for effective stimulation of cell division and production of maximum cell yield in Euglena gracilis Klebs cultured in vitro. Results indicate that two hormones combined exert more effective growth stimulation than a single horm...
Article
Full-text available
The combined effect of anther incubation time on CP induction medium (12, 14 and 16 days) and 2 concentrations of kinetin in R1 regeneration medium (0.1 and 0.3 mg/L) on the effectiveness of androgenesis was investigated in 17 genotypes of Capsicum grown in Poland. Plant material consisted of breeding lines and intraspecific hybrids of C. annuum, t...
Data
Full-text available
One of the key factors determining the effectiveness of pepper anther cultures is donor plant genotype. The stock material for androgenic embryos inductions is usually made up of hybrid forms, since the higher the degree of heterozygosity, the greater the chances of producing regenerates with unique genotypes. The aim of the presented research was...
Article
Full-text available
Eriophorum vaginatum L. is a promising spe-cies for phytostabilization, restoration, or creation of wet-lands, because it can survive in cold, nutrient-poor, or metal-contaminated soils. However, its propagation on a large scale is problematic due to the infrequent production of viable seeds, seed dormancy, and the limitations of reproduction by rh...
Article
Full-text available
The aim of the research was the analysis of 11 DH-R 2 pepper (Capsicum annuum L.) lines, obtained in anther cultures of '(ATZ1 × PO)' F 1 , '(ATZ1 × CDT)' F 1 and '(ATZ1 × TG)' F 1 hybrids. To determine the genetic homogeneity of anther-derived lines, the biometrical characteristics of fruits, as well as fruit colour and shape inheritance, were ana...
Data
Full-text available
Eight anther-derived DH lines of pepper hybrids: two red-fruited (AP14, AP15) and two yellow lines (AP25 and AP32) of C. annuum (ATZ1 × PO)F2, two lines of (C. frute-scens × C. annuum)F1 (FA1, FA2), and two of (C. frutescens × C. chinense)F1 (FCH2, FCH3), were studied regarding im-portant morphological plant and fruit characters. C. annuum breeding...
Conference Paper
Full-text available
Eriophorum vaginatum L. (cottongrass; Cyperaceae), an ecologically important tussock-forming perennial graminoid, is the most abundant herbaceous species in Northern Hemisphere peatlands and circumpolar tussock tundra. It possesses a high adaptability to a broad range of environmental conditions, and is widely distributed in nutrient-poor sites. Ad...
Conference Paper
Full-text available
Aluminum (Al) is the third most abundant element within the earth’s crust. Al pollutes air, water and soil, and is especially common in the highly acidified environments (with a pH of less than 5.0), where it can be very toxic to plants. The wetland ecosystems around Sudbury (ON, Canada) are extremely acidified with sulphur dioxide emissions and co...
Conference Paper
Full-text available
In vitro induced androgenesis is the formation of sporophyte from immature pollen grains in anther or isolated microspore cultures. It is a fast and efficient method of obtaining haploid plants, widely applied in breeding of many vegetable and ornamental species. Because of a single set of chromosomes, haploids are a precious material for basic gen...
Article
Full-text available
The present study evaluated the individual plants reaction of F2 hybrid generation of C. annuum: ATZ1×PO and ATZ1×CDT as well as two interspecific hybrids: C. frutescens×C. annuum ATM1 and C. frutescens×C. chinense on androgenesis conditions in in vitro anther cultures. The experiment was carried out following a modified method of Dumas de Vaulx et...
Data
Full-text available
Wstęp Androgeneza in vitro, indukowana w kulturach pylników, jest podstawową metodą otrzymywania roślin haploidalnych, a następnie linii podwojonych haploidów papryki (linie DH). Stanowią one bardzo cenny materiał hodowlany, poniewaŜ posiadają w pełni homozygotyczny układ genów. Przed włączeniem linii podwojonych haploidów do dalszych prac hodowlan...
Article
In the reported experiment, the effect of culture media composition on the effectiveness of androgenesis in anther culture of selected pepper (Capsicum) breeding lines and hybrids was tested. Anther culture was conducted according to Dumas de Vaulx et al. (1981), with slight modifications concerning the level of kinetin in R1 medium (0.1, 0.2, 0.3,...
Chapter
Full-text available
The aim of the research was to estimate the frequency of haploid formation through androgenesis and polyembryony, in cultivated and wild genotypes of Capsicum genus. Three cultivated forms of C. annuum: ATM 1, PO, CDT, C. frutescens and interspecific hybrid (C. frutescens × C. annuum) F1 were investigated. Anther culture was maintained according to...
Article
Full-text available
The primary aim of the study was to establish the effectiveness of induced androgenesis in in vitro anther culture of two pepper (Capsicum annuum L.) breeding lines--ATZ1 and PO, and a hybrid between these two lines (ATZ1 x PO)F1. Anther culture was maintained according to the method developed by Dumas de Vaulx et al. (1981) with some modifications...
Article
Full-text available
The aim of the research was to make a preliminary determination of the effectiveness of the induction of haploids in Capsicum frutescens L. In order to induce androgenesis red and yellow fruit forms of species were used, each bred by the researchers on their own. The experiment was performed in October. Anther cultures were conducted according to a...
Article
The aim of this research was to make a preliminary determination of the effectiveness of androgenesis of Capsicum frutescens L. This species is a source of a lot of interesting features and therefore should be wider included in pepper breeding programmes,what gives the opportunity of greater genetic variability. anther culture of red- and yellow-fr...

Questions

Questions (2)
Question
I would like to perform a field experiment testing the effect of beneficial endophytic bacteria on plants. I would like to deliver bacteria to the seeds in the form of powder inoculant, after mixing bacteria suspension with the carrier. I am not sure what materials are a suitable to use as a carrier; how I can prepare the carrier before bacteria can be mixed with it (grinding, sieving, sterilization, etc.) ; what volume ratio of carrier to bacteria suspension is optimal, what proportions of the inoculant vs..

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