Anna Patrícya Florentino

Wageningen University & Research | WUR · Laboratory of Microbiology

The physiological adaptability of microorganisms thriving by respiration of sulfur compounds and generating sulfide as an end product makes them an attractive tool for biotechnological applications in a variety of settings. Such versatility entices the scientific community interest; and, therefore, engineering and microbiological techniques have be...

An analytical method was developed to quantify a mixture of acetaminophen, metoprolol, methylparaben, carbamazepine, naproxen, estrone, estradiol, diclofenac, benzophenone, ibuprofen, progesterone, and mefenamic acid from domestic wastewater samples. To match fast and efficient chromatographic separation for different classes of compounds, an autom...

Medication waste is a concern for today’s society, from both environmental and public health perspectives. This study aims to contribute to the issue by presenting a mixed-methods approach to the problem through reverse logistics. First, a systematic literature review was performed to map the literature and identify the best practices implemented w...

This study compared the rates and microbial community development in batch bioassays on autotrophic denitrification using elemental sulfur (S0), pyrite (FeS2), thiosulfate (S2O32–), and sulfide (S2–) as electron donor. The performance of two inocula was compared: digested sludge (DS) from a wastewater treatment plant of a dairy industry and anaerob...

This research assessed the microbiological suitability of oleate degradation coupled to sulfidogenesis by enriching communities from anaerobic sludge treating dairy products with S0, SO 3 2 - , SO 4 2 - , and S2 O 3 2 - as electron acceptors. The limiting factor hampering highly efficient oleate degradation was investigated in batch r...

The current study was conducted to investigate the effects of mixing ratios on anaerobic co-digestion of blackwater and kitchen organic waste. The biological methane potential (BMP) of co-digested blackwater and kitchen waste were 0.85 ± 0.07 and 0.83 ± 0.06 at volatile solids (VS) ratios of 1:2 and 1:3 whereas the BMP of blackwater alone was 0.34...

The effect of phosphate on anaerobic ammonium oxidation (anammox) was investigated in an integrated fixed film activated sludge-sequencing batch reactor (IFAS-SBR) treating raw digester sludge thickening lagoon supernatants before and after Ostara® treatment (for phosphorus recovery). At a similar nitrogen loading rate (NLR) of 0.31 kg N/(m3·d), pr...

This study investigated the effect of granular activated carbon (GAC) on the digestion of blackwater collected from different collection systems, by monitoring the biochemical methane production (BMP), adsorption of molecules to GAC and their impacts on the microbial community. Without GAC-amendment, BMP reached 35.6, 42.6 and 50.4% in 1L, 5L and 9...

This work aimed to investigate algal diversity at the genus level in stabilization pond systems treating domestic wastewater and to evaluate the feasibility of an electroflotation by alternate current (EFAC) system for simultaneous microalgae separation and cell disruption. Evaluation of algal diversity showed that the genera Euglena and Chlorella...

Source diverted blackwater (i.e., toilet wastewater with or without food waste residuals) represents an ideal waste stream for bioenergy recovery through anaerobic digestion. However, due to the high free ammonia concentration contained in blackwater collected from water conserving toilets (e.g., vacuum toilets), CH4 recovery can be significantly r...

The source diverted blackwater treatment is receiving growing attention as an alternative to conventional energy intensive wastewater management and treatment systems. Blackwater, containing concentrated organic materials, can be anaerobically digested to recovery bioenergy. However, the methane recovery from blackwater is often inhibited by the pr...

Methanogenesis and enrichment of microorganisms capable of interspecies electron and/or hydrogen exchange was investigated with addition of granular activated carbon (GAC) to batch anaerobic digesters treating vacuum collected blackwater with high ammonia concentration. The biochemical methane potential increased from 34% to 53%, while chemical oxy...

Partial nitrification was developed in this study for the sidestream treatment of high ammonium strength digested sludge supernatant in an integrated fixed film activated sludge (IFAS) reactor operating under sequencing batch mode. This paper elucidated the impacts of feed water characteristics on microbial population dynamics and kinetics in a par...

Figure S1. Sulfide production levels of Desulfurella amilsii cultures incubated under different reductive conditions.

Table S1. Unique proteins detected per condition analyzed. The locus tag for the genes encoded in D. amilsii is DESAMIL20_*. To avoid repetition of the prefix in the tables, the locus tags are represented only by the specific identifier. Table S2. Differentially expressed proteins in a pairwise comparison Acet_S_6.5 vs Acet_thio_6.5. Positive valu...

Biosulfidogenesis can be used to remediate low pH and high metal content waters such as acid mine drainage and recover the present metals. The selection of a cheap electron donor for the process is important for the economic viability. In this work we isolated a novel versatile acidotolerant fermentative bacterium (strain ALET) that is able to use...

Many questions regarding proteins involved in microbial sulfur metabolism remain unsolved. For sulfur respiration at low pH, the terminal electron acceptor is still unclear. Desulfurella amilsii is a sulfur‐reducing bacterium that respires elemental sulfur (S⁰) or thiosulfate, and grows by S⁰ disproportionation. Due to its versatility, comparative...

Source-diverted blackwater (toilet wastewater) contains most of the organic energy in domestic wastewater and can be treated anaerobically to maximize energy recovery. Blackwater collected from toilets of different water saving options (e.g., conventional, dual and vacuum toilets) represents different characteristics, but their digestibility has no...

Municipal wastewater contains valuable organic contents that can be recovered as energy through anaerobic digestion. The operating temperature impacts the design and performance of anaerobic digestion systems and can be selected to accommodate different geographical zones, from tropical to frigid. Here the biological methane potential (BMP) and the...

The acidotolerant sulfur reducer Desulfurella amilsii was isolated from sediments of Tinto River, an extremely acidic environment. Its ability to grow in a broad range of pH and to tolerate certain heavy metals offers potential for metal recovery processes. Here we report its high-quality draft genome sequence and compare it to the available genome...

Sulfur-reducing prokaryotes play an important role in the sulfur biogeochemical cycle, especially in deep-sea vents, hot springs and other extreme environments. The reduction of elemental sulfur is not very favorable thermodynamically, but still provides enough energy for growth of microorganisms. Currently known sulfur reducers are spread over abo...

A novel acidotolerant and moderately thermophilic sulfur-reducing bacterium was isolated from sediments of the Tinto River (Spain), an extremely acidic environment. Strain TR1T stains Gram-negative, is obligately anaerobic, non-spore forming and motile. Cells are short rods (1.5-2 by 0.5-0.7 µm),appearing single or in pairs. Strain TR1T is catalase...

Microbiological suitability of acidophilic sulfur reduction for metal recovery was explored by enriching sulfur reducers from acidic sediments at low pH (from 2 to 5) with hydrogen, glycerol, methanol and acetate as electron donors at 30°C. The highest levels of sulfide in the enrichments were detected at pH 3 with hydrogen and pH 4 with acetate. C...

This study investigates the effects of essential oil of Pterodon polygalaeflorus (EOPP) and β-caryophyllene (β-CAR). EOPP and β-CAR relaxed the basal tone of ileum smooth muscle in a concentration-dependent manner (IC(50) s = 394.35 ± 62.12 and 68.65 ± 9.51 μg/mL respectively), an effect that was unaltered by hexamethonium, L-nitroarginine methyl e...