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Introduction
animal reproduction, semen conservation, sperm quality, sperm metabolism
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Publications
Publications (88)
The aim of this study was to investigate the effect of season (breeding vs. non-breeding) on the characteristics of warmblood stallion spermatozoa during liquid storage. Ejaculates were collected from eight stallions during the breeding and non-breeding seasons (March–July and September–December, respectively) and were diluted in an EquiPro extende...
The study aimed to examine the parameters of turkey ejaculates (n=40) of high and low quality (HQ and LQ, respectively), preserved with the addition of taurine (TAU; 5 mM, 10 mM) and ergothioneine (EGT; 5 mM, 10 mM) for 48 h at 5°C in a liquid state. The motility, plasma membrane integrity (PMI), mitochondrial function, apoptotic and necrotic sperm...
Simple Summary
The choice of the optimal sperm preservation method is an important consideration in animal breeding. Stored semen can be used for reproductive purposes to introduce new genotypes and prevent inbreeding, which poses a considerable problem in cervid farms. The aim of this study was to evaluate and compare the effect of storage time an...
The aim of the study was to assess the impact of solarium light therapy on selected biological and biochemical parameters of peripheral blood in recreational horses. The study involved 10 horses divided into two groups of young (aged 5 to 7 years) and old (aged 14 to 19 years) individuals. All animals participated in light therapy sessions every ot...
The biological properties of European red deer (Cervus elaphus elaphus) spermatozoa stored in the epididymides and in a liquid state were compared. Spermatozoa were collected from the
epididymides harvested post-mortem. In the first variant, spermatozoa were diluted in two extenders (Bovidyl® and Salomon’s), and were stored at 5 ◦C for up to 144 h....
Animal fertility is the result of a combination of genetic, physiological and environmental factors. Assessment of semen quality plays a key role in determining the reproductive performance of boars. The aim of the study was to determine the effect of two seasons of the year on the morphology, morphometry, cell membrane integrity and mitochondrial...
Simple Summary
Flow cytometry (FC) is the recommended technique for assessing sperm quality. In comparison with fluorescence microscopy (FM), FC supports analyses of much larger sperm populations and generates more reliable results. However, FC is not always accessible, and sperm populations are often evaluated with the use of FM. In the present st...
Recent advances in assisted reproductive technology (ART) have increased the effectiveness of fertility treatments [...]
The aim of this study was to assess the quality and fertilizing potential of red deer epididymal spermatozoa stored in a liquid state for up to 11 days (D11). In Experiment 1, sperm quality was determined. In Experiment 2, the efficiency of in vitro fertilization (IVF) and artificial insemination (AI) of stored sperm were evaluated. An analysis of...
Simple Summary
The use of commercial extenders for storing cervid epididymal spermatozoa in a liquid state can contribute to popularizing this method of semen preservation in cervid farms. The aim of this study was to assess the effect of various extenders (Bovidyl®, BoviFree®, and BioXcell®) on the quality of European red deer epididymal spermatoz...
The aim of this study was to assess the quality and fertilizing potential of red deer epididymal spermatozoa stored in a liquid state for up to 11 days (D11). In Experiment 1, sperm quality was determined. In Experiment 2, the efficiency in vitro fertilization (IVF) and artificial insemination (AI) of stored sperm were evaluated. An analysis of spe...
Spermatozoa derived from yellow semen (YS) are considered to
be those with lower quality and reduced fertilization capacity. We
investigated the differences in total motility (TMOT) (CASA sys-
tem), plasma membrane integrity (PMI) (SYBR-14/PI), mitochon-
drial potential (MP) (JC-1/PI) and activity of antioxidant enzymes:
superoxide dismutase (SO...
Most reports on the effect of freezing-thawing procedure on the
sperm of red deer concern the Iberian deer. This study investigated
the effect of cryopreservation on motility, membrane and DNA in-
tegrity of epididymal sperm of European red deer. Samples of sperm
were obtained post mortem from cauda epididymis (from 10 bucks).
Samples were froz...
Celem przeprowadzonych badań była ocena ruchliwości oraz zmian morfologicznych plemników najądrzowych jelenia szlachetnego przechowywanych w komercyjnych rozcieńczalnikach w temperaturze 5°C.
Próby plemnikowe pobrano pośmiertnie z ogona najądrzy (od 4 byków jelenia szlachetnego) i rozrzedzono w rozcieńczalnikach: BioXcell (lMV Technologies, Francja...
Na jakość przechowywanego nasienia zwierząt wpływa wiele czynników m. in. skład rozcieńczalnika, czas i temperatura przechowywania. Przechowywanie nasienia w stanie płynnym w odpowiedniej temperaturze może znacznie przedłużyć żywotność i funkcjonalność plemników. Celem prowadzonych badań była analiza ruchliwości, żywotności i zmian apoptotycznych n...
The aim of the study was to evaluate viability longevity and quality of liquid-stored epididymal sperm of European red deer (Cervus elaphus elaphus). Sperm samples were recovered from epididymides of 15 mature stags. Samples were diluted to 100 × 106 sperm⁄mL in modified Salomon's extender and stored at 5 °C for 25 days. Sperm were analyzed to dete...
The red deer shows a seasonal reproductive pattern shaped by pho-toperiod, and is considered to be sexually active from September to November, and in seasonal anestrus for the rest of the year. The pattern of the steroids estradiol (E2) and progesterone (P4) reflect reproductive activity. The aims of the study were the estimation of P4 and E2 profi...
The aim of this study was to identify the proteoforms of albumin and kallikrein in stallion seminal plasma (SP), and to determine their correlations with sperm motility parameters. The experimental material consisted of ejaculates from 8 stallions, which were collected during the breeding and non-breeding seasons (BS and NBS, respectively). SP prot...
The objective of this study was a comparative analysis of the impact of the addition of lipoproteins from ostrich egg yolk (LPFo) and hen egg yolk (HEY) to a Tris-citric acid-fructose (TCF) extender on the quality of dog sperm preserved at 5 °C for 7 days. The sperm-rich fraction of the ejaculate was manually collected and extended using the follow...
The aim of this study was to evaluate the effect of lipoprotein fraction isolated from ostrich egg yolk (LPFo) on the metabolic activity of boar spermatozoa following liquid semen storage in different extenders and temperatures. Boar ejaculates were extended in Androhep, BTS, and MR-A without (control) or with the addition of LPFo and stored for th...
The aim of this study was to evaluate the effect of lipoprotein fraction isolated from ostrich egg yolk (LPFo) on the metabolic activity of boar spermatozoa following liquid semen storage in different extenders and temperatures.
Boar ejaculates were extended in Androhep, Beltsville thawing solution (BTS), and Martín-Rillo and Alias (MR-A) without (...
The aim of this study was to investigate the effect the sperm-rich fraction (F1) and the post-F1 fraction (F2) on the quality of boar spermatozoa stored in a liquid state. Ejaculates were collected from three Polish Landrace boars. Each ejaculate fraction was diluted with BTS short-term extender and Safe-Cell Plus (SCP) long-term extender and store...
Wprowadzenie: Na jakość przechowywanych plemników knura wpływa wiele czynników m.in. rozcieńczalnik, temperatura, czas przechowywania i frakcja ejakulatu. Cel pracy: Celem badań było określenie wpływu frakcji ejakulatu (gęstej i pozostałej) na parametry ruchu plemników knura przechowywanych w stanie płynnym. Materiał i metody: Frakcje ejakulatu roz...
Wprowadzenie: Białka plazmy nasienia pełnią kluczowe funkcje związane z regulacją procesu zapłod-nienia. Badania zmian w poziomie ekspresji białek plazmy nasienia pozwalają na identyfikację protein związanych z płodnością samca. Cel pracy: Celem przeprowadzonych badań była analiza zmian profili białkowych plazmy nasienia ogiera w zależności od wiek...
The aim of the study was to determine the effects of platelet-activating factor (PAF) on selected quality parameters of cryopreserved bull semen with reduced sperm motility used for artificial insemination. The aim of experiment 1 was to identify the optimal concentration of the phospholipid able to preserve sperm viability. Cryopreserved semen was...
Contents
The objective of this study was to make the preliminary characterization of the antioxidant defence systems of the yellow fraction ( YF ) of red deer's ( Cervus elaphus L.) semen during the rutting period. The semen was collected using artificial vagina ( AV ). The studies included spectrophotometric determination of antioxidant enzymes ac...
The aim of this study was to investigate the effects of storage of semen in different commercial extenders on the pre-freezing and post-thawing quality of boar spermatozoa. Semen was diluted in BTS, Androhep (AH) and Gedil (GD), stored for 24 h at 17°C, and then frozen in accordance with the cryopreservation protocol. Analyses of the quality of spe...
This study investigated the effects of semen storage in different extenders, prior to freezing, on post-thaw sperm quality characteristics. Boar ejaculates were diluted in BTS, Androhep (AH, Minitübe) and Gedil (GD, Genes Diffusion) extenders, stored for 24h at 17 degrees C, and frozen using a standard cryopreservation protocol. Sperm assessments i...
The aim of the study was to analyse sperm proteomes of ejaculates from Polish Large White (PLW) and Polish Landrace (PL) boars and to identify differences which putatively influence semen quality. Spermatozoa protein profiles were analysed by electrophoretic methods followed by selected techniques to evaluate semen quality on the following factors:...
Zinc-binding proteins (ZnBPs) isolated from boar seminal plasma exert multiple effects on boar
spermatozoa. The aim of this study was to determine whether the isolated zinc-binding proteins were
capable of binding additional ligands. The affinity chromatography method was used for the first
time to demonstrate that boar ZnBPs show affinity for both...
This study aimed to investigate the effect of seasonal variations on the metabolic characteristics of fresh and extended stallion spermatozoa. Ejaculates, collected from 4 stallions (aged 10 to 20 years) during the breeding and non-breeding seasonal periods (March-July and September-December, respectively), were diluted in an EquiProTM extender (Mi...
This study investigated seasonal changes in the metabolic performance of spermatozoa and activity of the antioxidant enzymes in the seminal plasma of three wild boar/domestic pigs (aged 1.5 to 2.5 years) and the activity of the antioxidant enzymes in fluids of the cauda epididymidis and vesicular glands from 16 wild boar/domestic pig hybrids (aged...
Sperm surface binding sites for non-zinc-binding proteins (nZnBPs) and zinc-binding proteins (ZnBPs) were studied by the fluorescence technique with biotin-labelled proteins. The nZnBPs binding pattern was unspecific, no characteristic sites on plasmalemma were found. ZnBPs were attached mainly to the acrosomal region of sperm head and to the sperm...
This study investigated seasonal changes in the metabolic performance of spermatozoa and
activity of the antioxidant enzymes in the seminal plasma of three wild boar/domestic pigs (aged 1.5 to
2.5 years) and the activity of the antioxidant enzymes in fluids of the cauda epididymidis and vesicular
glands from 16 wild boar/domestic pig hybrids (aged...
Semen quality assessment methods are very important in predicting the fertilizing ability of persevered spermatozoa and to improve animal reproductive technology. This review discusses some of the current laboratory methods used for semen quality assessments, with references to their relevance in the evaluation of male fertility and semen preservat...
This study was aimed to analyze the metabolic activity and membrane integrity of boar spermatozoa following storage in long-term semen extenders. Boar semen was diluted with Androhep EnduraGuard (AeG), DILU-Cell (DC), SafeCell Plus (SCP) and Vitasem LD (VLD) extenders and stored for 10 days at 17 degrees C. Parameters of the analyzed sperm metaboli...
Zinc ions and zinc-protein ligands play an essential role in the reproductive process in mammals. Recently, we have been isolated and characterized zinc-binding proteins (ZnBPs) and non-zinc-binding proteins (nZnBPs) of canine seminal plasma. The aim of this study was to investigate the effect of the ZnBPs and nZnBPs fractions on motility and acros...
This study aimed to investigate the effects of seasonal changes in the metabolic status of spermatozoa and antioxidant enzyme activity of the fluids of the vesicular glands and cauda epididymidis, and in the seminal plasma of the wild boar/domestic pig hybrids. Besides sperm motility and plasma membrane integrity assessments, parameters of the sper...
Boar variability affects sperm metabolism activity in liquid stored semen at 5°C
Metabolic activity of boar spermatozoa, liquid stored for three days at 5°C, was measured using bioluminescence for ATP content, fluorescent assay (JC fluorochrome) of mitochondrial activity and oxygen consumption. Sperm motility and plasma membrane integrity (PMI) wer...
Effect of platelet activating factor (PAF) supplementation in semen extender on viability and ATP content of cryopreserved canine spermatozoa
The aim of this study was to investigate the effect of platelet activating factor (PAF) on the quality characteristics of cryopreserved canine spermatozoa. Cryopreserved semen of 5 mixed-breed dogs was treate...
Energy produced by the sperm mitochondria is used for motility, capacitation, hyperactivation and acrosome reaction, as well as for the transport of substrates across the membranes. Hence, evaluation of the mitochondrial function of spermatozoa during liquid semen storage is necessary to assess their viability prior to artificial inseminations. The...
The sperm plasma membrane integrity is involved in metabolic exchanges with the surrounding medium and plays an important role in several fertilization related events. Differences in the sperm cryotolerance between individual boars might be one of the sources of variability that could affect the fertilizing ability of frozen-thawed spermatozoa. In...
Celem badań było określenie wpływu dializy nasienia knura jako jednego z etapów postępowania technologicznego przed jego zamrażaniem na wybrane parametry oceny funkcjonalności mitochondriów plemników. Analizy zmian zawartości ATP w plemnikach określano metodą bioluminescencyjną adoptowaną do plemników knura. Integralność błon plazmatycznych w różny...
This study investigated the effects of different temperatures (5º and 16ºC) on the metabolic activity of boar spermatozoa preserved in a standard semen extender, Kortowo 3 (K3), supplemented with or without egg yolk lipoprotein fractions (LPF) isolated from hen or ostrich egg yolk. Besides motility and plasma membrane integrity (PMI) assessments of...
Low-molecular weight components of the seminal plasma have a detrimental effect on sperm function. The present study was undertaken to evaluate the effect of the removal of low-molecular weight components by dialysis on sperm characteristics prior to and after freezing. Semen, collected from 5 boars, was extended in Kortowo-3 extender (K-3, Poland)...
This study investigated the effects of the supplementation of lipoprotein fractions isolated from ostrich egg yolk (LPFo) to extender on sperm charactertics during liquid storage (5ºC and 16ºC) or cryopreservation of boar semen. Deterioration in the sperm characteristics, such as motility, plasma membrane integrity (CFDA/PI) and mitochondrial funct...
The aim of this study was to investigate the effect of providing a supplement containing polyunsaturated fatty acids and antioxidants (PROSPERM) on the biochemical characteristics of boar semen. Two sexually mature boars were fed a standard diet with PROSPERM (250 g daily) for a 24-week period. Ejaculates collected prior to supplementation were use...
La yema de huevo ha sido el componente más común de los diluyentes desde que se descubrió su acción protectora contra el choque frío en espermatozoides de toro suspendido en una solución tampón fosfato (Philips, 1939). Se ha confirmado que las fracciones lipoproteicas de los fosfolípidos de la yema de huevo protegen a los espermatozoides contra el...
The aim of this study was to investigate the effects of supplementing boars’ diet with specific polyunsaturated fatty AIDS (PUFAs) and antioxidants (PROSPERM®) on semen characteristics and subsequent liquid preservation. The control group consisted of boars receiving a standard diet, whereas boars of the experimental group received the nutritional...
