Anna Clara Milesi Galdino

Cedars-Sinai Medical Center · Department of Pathology and Laboratory Medicine

Tackling microbial resistance requires continuous efforts for the development of new molecules with novel mechanisms of action and potent antimicrobial activity. Our group has previously identified metal-based compounds, [Ag(1,10-phenanthroline-5,6-dione)2]ClO4 (Ag-phendione) and [Cu(1,10-phenanthroline-5,6-dione)3](ClO4)2.4H2O (Cu-phendione), with...

Denture-related stomatitis caused by Candida spp. affects elderly individuals using partial/total prosthesis, provoking several discomforts including burning sensation and altered taste. Herein, we have studied 52 denture-wearing individuals (>60 years-old), attended at the dentistry clinic of UNIVALE, aiming to isolate Candida spp. directly from t...

Therapeutic options are limited for patients infected with Acinetobacter baumannii due to its multidrug-resistance profile. So, the search for new antimicrobials against this gram-negative bacterial pathogen has become a worldwide priority. The present study aimed to evaluate the effects of 1,10-phenanthroline (phen), 1,10-phenanthroline-5,6-dione...

Hydrazide ligand, (Z)-N’-(6-oxo-1,10-phenanthrolin-5(6H)-ylidene)isonicotinohydrazide, 1 forms from a 1:1 Schiff base condensation reaction between isoniazid (INH) and 1,10-phenanthroline-5,6-dione (phendione). Ag+ and Mn2+ complexes with 1:2 metal:ligand stoichiometry are prepared:[Ag(1)2]NO3, [Ag(1)2]BF4 and [Mn(1)2](NO3)2. Polymeric {[Ag(1)(NO3)...

Enteroaggregative Escherichia coli (EAEC) is an emerging pathotype responsible for acute and persistent diarrhea. It can be classified as typical and atypical strains, respectively, based on the presence or absence of the AggR regulon, suggesting a higher virulence for typical EAEC. This study aimed to evaluate in the Galleria mellonella model if t...

Elastase B (lasB) is a multifunctional metalloenzyme secreted by the gram-negative pathogen Pseudomonas aeruginosa, and this enzyme orchestrates several physiopathological events during bacteria-host interplays. LasB is considered to be a potential target for the development of an innovative chemotherapeutic approach, especially against multidrug-r...

Anti-virulence strategy metric. A search in PubMed database (www.pubmed.com) was conducted on May 14th, 2018, using the keywords "anti-virulence". For the analysis, only papers published in English containing this word in the title or abstract were considered just. The graph shows that the number of anti-virulence publications is sharply increasing...

Pseudomonas aeruginosa is a non-fermentative, gram-negative bacterium that is one of the most common pathogens responsible for hospital-acquired infections worldwide. The management of the infections caused by P. aeruginosa represents a huge challenge in healthcare settings due to the increased emergence of resistant isolates, some of them resistan...

Biofilm is the preferential growth lifestyle of many microorganisms, including both bacterial and fungal human pathogens. A biofilm is considered a strong and dynamic structure, which confers a broad range of advantages to the microorganisms such as adhesion/cohesion capabilities, mechanical properties, nutritional sources, metabolites’ exchange pl...

Background Pseudomonas aeruginosa is an important human pathogen that causes severe infections in a wide range of immunosuppressed patients. Herein, we evaluated the proteolytic profiles of 96 Brazilian clinical isolates of P. aeruginosa recovered from diverse anatomical sites. Methods Cell-associated and extracellular proteases were evidenced by...

Pseudomonas aeruginosa is a ubiquitous and opportunistic human pathogen that represents a critical problem to the clinician due to the increased number of resistant strains isolated from hospital settings. In addition, there is a great variety of pathologies associated with this versatile Gram-negative bacterium. P. aeruginosa cells are able to pro...

Background: Pseudomonas aeruginosa is an important human pathogen that causes severe infections in a wide range of immunosuppressed patients. Herein, we evaluated the proteolytic profiles of 96 Brazilian clinical isolates of P. aeruginosa recovered from diverse anatomical sites. Methods: Cell-associated and extracellular proteases were evidenced b...

Denture stomatitis, also known as atrophic candidiasis, remains the most frequent form of oral candidiasis, being detected in approximately 25%-65% of denture-wearing patients. Denture stomatitis is characterized by an erythematous inflammation of mucosal areas covered by prosthesis with preferential localization in the palatal mucosa. Although the...

Objectives: The beneficial antimicrobial properties of 1,10-phenanthroline (phen)-based drugs, together with the imperative need to develop new chemotherapeutic options for prevention/treatment of infections caused by MDR Gram-negative bacteria, led us to evaluate the effects of phen, 1,10-phenanthroline-5,6-dione (phendione), [Ag(phendione)2]ClO4...