Anna Campalans

Anna Campalans
Atomic Energy and Alternative Energies Commission | CEA · Institute of cellular and molecular radiation biology (iRCM)

PhD

About

46
Publications
6,489
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1,175
Citations
Citations since 2016
20 Research Items
618 Citations
2016201720182019202020212022020406080100120
2016201720182019202020212022020406080100120
2016201720182019202020212022020406080100120
2016201720182019202020212022020406080100120

Publications

Publications (46)
Article
Many endocrine disruptors have been proven to impair the meiotic process which is required for the production of healthy gametes. Bisphenol A is emblematic of such disruptors, as it impairs meiotic prophase I and causes oocyte aneuploidy following in utero exposure. However, the mechanisms underlying these deleterious effects remain poorly understo...
Article
Full-text available
Cancer stem cells (CSCs) serve an essential role in failure of conventional antitumor therapy. In breast cancer, CD24‑/low/CD44+ phenotype and high aldehyde dehydrogenase activity are associated with CSC subtypes. Furthermore, CD24‑/low/CD44+ pattern is also characteristic of mesenchymal cells generated by epithelial‑mesenchymal transition (EMT). C...
Preprint
Full-text available
The DNA-glycosylase OGG1 oversees the detection and clearance of the 7,8-dihydro-8-oxoguanine (8-oxoG), which is the most frequent form of oxidized base in the genome. This lesion is deeply buried within the double-helix and its detection requires careful inspection of the bases by OGG1 via a mechanism that remains only partially understood. By ana...
Preprint
There is compelling evidence that cancer stem cells (CSCs) play an essential role in failure of conventional antitumor therapy. In breast cancer, CD24-/low/CD44+ phenotype as well as a high aldehyde dehydrogenase activity (ALDH+) are widely associated with CSC subtypes. Furthermore, CD24-/low/CD44+ pattern is also characteristic of the mesenchymal...
Article
Full-text available
Double-strand breaks (DSBs) are harmful lesions and a major cause of genome instability. Studies have suggested a link between the nuclear envelope and the DNA damage response. Here, we show that lamin B1, a major component of the nuclear envelope, interacts directly with 53BP1 protein, which plays a pivotal role in the DSB repair. This interaction...
Article
Full-text available
The most frequent DNA lesion resulting from an oxidative stress is 7,8-dihydro-8-oxoguanine (8-oxoG). 8-oxoG is a premutagenic base modification due to its capacity to pair with adenine. Thus, the repair of 8-oxoG is critical for the preservation of the genetic information. Nowadays, 8-oxoG is also considered as an oxidative stress-sensor with a pu...
Article
Full-text available
Background: Functional genomics employs several experimental approaches to investigate gene functions. High-throughput techniques, such as loss-of-function screening and transcriptome profiling, allow to identify lists of genes potentially involved in biological processes of interest (so called hit list). Several computational methods exist to ana...
Preprint
Full-text available
As many endocrine disruptors, Bisphenol A is emblematic as it impairs meiotic prophase I and causes oocyte aneuploidy following in utero exposure. Here, we investigated the effect on oogenesis in mouse of fetal exposure to two BPA analogs, Bisphenol A Diglycidyl Ether or Bisphenol AF. These analogs delay meiosis initiation, increase MLH1 foci per c...
Article
Full-text available
One of the most abundant DNA lesions induced by oxidative stress is the highly mutagenic 8-oxoguanine (8-oxoG), which is specifically recognized by 8-oxoguanine DNA glycosylase 1 (OGG1) to initiate its repair. How DNA glycosylases find small non-helix-distorting DNA lesions amongst millions of bases packaged in the chromatin-based architecture of t...
Article
Full-text available
Today, 3D imaging techniques are emerging, not only as a new tool in early drug discovery but also for the development of potential therapeutics to treat disease. Particular efforts are directed towards in vivo physiology to avoid perturbing the system under study. Here, we assess non-invasive 3D lensless imaging and its impact on cell behavior and...
Article
Full-text available
Repair of two major forms of DNA damage, single strand breaks and base modifications, are dependent on XRCC1. XRCC1 orchestrates these repair processes by temporally and spatially coordinating interactions between several other repair proteins. Here we show that XRCC1 contains a central DNA binding domain (CDB, residues 219–415) encompassing its fi...
Article
Full-text available
Transcription-coupled nucleotide excision repair factor Cockayne syndrome protein B (CSB) was suggested to function in the repair of oxidative DNA damage. However thus far, no clear role for CSB in base excision repair (BER), the dedicated pathway to remove abundant oxidative DNA damage, could be established. Using live cell imaging with a laser-as...
Article
Accumulation of 8-oxoguanine (8-oxoG) in mitochondrial DNA and mitochondrial dysfunction have been observed in cells deficient for the DNA glycosylase OGG1 when exposed to oxidative stress. In human cells, up to eight mRNAs for OGG1 can be generated by alternative splicing and it is still unclear which of them codes for the protein that ensures the...
Article
Accumulation of 8-oxoG in mtDNA and mitochondrial dysfunction have been observed in cells deficient for the DNA glycosylase OGG1 exposed to oxidative stress. In human cells up to eight mRNAs for OGG1 can be generated by alternative splicing and it is still unclear which of them codes for the protein that ensures the repair of 8-oxoG in mitochondria...
Preprint
Functional genomics employs several experimental techniques to investigate gene functions. These techniques such as loss-of-function screening and transcriptome profiling performed in a high-throughput manner give as result a list of genes involved in the biological process of interest. There exist several computational methods for analysis and int...
Article
Full-text available
Abstract Motivation: Incorporating gene interaction data into the identification of ‘hit’ genes in genomic experiments is a well-established approach leveraging the ‘guilt by association’ assumption to obtain a network based hit list of functionally related genes. We aim to develop a method to allow for multivariate gene scores and multiple hit lab...
Article
Full-text available
Phenotypic screening monitors phenotypic changes induced by perturbations, including those generated by drugs or RNA interference. Currently-used methods for scoring screen hits have proven to be problematic, particularly when applied to physiologically relevant conditions such as low cell numbers or inefficient transfection. Here, we describe the...
Article
Full-text available
E2F transcription factors regulate a wide range of biological processes, including the cellular response to DNA damage. In the present study, we examined whether E2F family members are transcriptionally induced following treatment with several genotoxic agents, and have a role on the cell DNA damage response. We show a novel mechanism, conserved am...
Article
XRCC1 is an essential protein required for the maintenance of genomic stability through its implication in DNA repair. The main function of XRCC1 is associated with its role in the single-strand break (SSB) and base excision repair (BER) pathways that share several enzymatic steps. We show here that the polymorphic XRCC1 variant R194W presents a de...
Article
Full-text available
Single-strand break repair (SSBR) and base excision repair (BER) of modified bases and abasic sites share several players. Among them is XRCC1, an essential scaffold protein with no enzymatic activity, required for the coordination of both pathways. XRCC1 is recruited to SSBR by PARP-1, responsible for the initial recognition of the break. The recr...
Article
Inhibition of DNA repair has been proposed as a mechanism underlying heat-induced sensitization of tumour cells to some anticancer treatments. Base excision repair (BER) constitutes the main pathway for the repair of DNA lesions induced by oxidizing or alkylating agents. Here, we report that mild hyperthermia, without toxic consequences per se, aff...
Article
Full-text available
How DNA repair machineries detect and access, within the context of chromatin, lesions inducing little or no distortion of the DNA structure is a poorly understood process. Removal of oxidized bases is initiated by a DNA glycosylase that recognises and excises the damaged base, initiating the base excision repair (BER) pathway. We show that upon in...
Article
Plants use a variety of small peptides for cell to cell communication during growth and development. Leguminous plants are characterized by their ability to develop nitrogen-fixing nodules via an interaction with symbiotic bacteria. During nodule organogenesis, several so-called nodulin genes are induced, including large families that encode small...
Article
The induction of mutations in mammalian cells exposed to cadmium has been associated with the oxidative stress triggered by the metal. There is increasing evidence that the mutagenic potential of Cd is not restricted to the induction of DNA lesions. Cd has been shown to inactivate several DNA repair enzymes. Here we show that exposure of human cell...
Article
Full-text available
The retina is highly exposed to oxidative stress due to the high level of oxygen consumption in this tissue and its exposure to light. The main DNA base lesion generated by oxygen free radicals is 8-oxoguanine (8-oxoG). However, its presence in retinal cells and the mechanisms underlying its repair remain undetermined. 8-oxoguanine DNA glycosylase...
Article
Full-text available
The DNA glycosylase hOGG1 initiates base excision repair (BER) of oxidised purines in cellular DNA. Using confocal microscopy and biochemical cell fractionation experiments we show that, upon UVA irradiation of human cells, hOGG1 is recruited from a soluble nucleoplasmic localisation to the nuclear matrix. More specifically, after irradiation, hOGG...
Article
Repair of chemically modified bases in DNA is accomplished through base excision repair (BER). This pathway is initiated by a specific DNA glycosylase that recognizes and excises the altered base to yield an abasic (AP) site. After cleavage of the AP site by APE1, repair proceeds through re-synthesis and ligation steps. In mammalian cells, the XRCC...
Article
Full-text available
In eukaryotes, diverse mRNAs containing only short open reading frames (sORF-mRNAs) are induced at specific stages of development. Their mechanisms of action may involve the RNA itself and/or sORF-encoded oligopeptides. Enod40 genes code for highly structured plant sORF-mRNAs involved in root nodule organogenesis. A novel RNA binding protein intera...
Article
Cinnamoyl-coenzyme A reductase (CCR, EC 1.2.1.44), cinnamyl alcohol dehydrogenase (CAD, EC 1.1.1.195) and caffeic O-methyl transferase (COMT, EC 2.1.1.68) are three enzymes of the biosynthetic pathway of lignin. This pathway has been mostly studied in dicots but is less well known in monocots. We report the histochemical localization of these enzym...
Chapter
The proceedings include the Johanna Döbereiner memorial lecture, a note on the discovery in 1965 of inorganic nitrogen complexes, the keynote address, and 68 papers in 15 sections (each mostly introduced by an overview): Chemistry and biochemistry of nitrogenase; Bacterial genomics; Plant genomics; Signal transduction; Developmental biology; Signal...
Article
Full-text available
We analyzed several genes that were strongly expressed in response to dehydration of almond (Prunus amygdalus (L.) Batsch) as a means of identifying and determining the genetic basis of mechanisms involved in drought tolerance. The advantages of using almond as a model system for studying dehydration tolerance in woody species include its small dip...
Article
The expression pattern of proteins was analysed during late embryogenesis in almond (Prunus amygdalus) to obtain information on the molecular events leading to the acquisition of the embryo desiccation tolerance. Analysis of the ABA content in developing embryos revealed high levels at 50 DAF with a rapid decrease at the onset of desiccation. Two-d...
Article
The expression pattern of proteins was analysed during late embryogenesis in almond (Prunus amygdalus) to obtain information on the molecular events leading to the acquisition of the embryo desiccation tolerance. Analysis of the ABA content in developing embryos revealed high levels at 50 DAF with a rapid decrease at the onset of desiccation. Two-d...
Article
The plant hormone abscisic acid (ABA) is necessary for the regulation of several aspects during seed development and for the response to environmental stresses such as desiccation, salt and cold. An important part of the physiological response to ABA is achieved through gene expression. Although different signal molecules have been identified, the...

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Projects (2)
Archived project
Project
Current priority directions: * consequences of DNA lesions for gene transcription in function of the lesion structure and position; * redundancy of the DNA repair pathways, determinants of the pathway choice and factors limiting the repair; * functional significance of the oxidation products of 5-methylcytosine