Anna Biernasiuk

Medical University of Lublin · Department of Pharmaceutical Microbiology with the Laboratory of Microbiological Diagnostics

Comparative analysis of flavonoids and phenolic acids composition, in plants of six species of Monarda from family Lamiaceae was carried out. The 70% (v/v) methanolic extracts of flowering herbs of Monarda citriodora Cerv. ex Lag., Monarda bradburiana L.C. Beck, Monarda didyma L., Monarda media Willd., Monarda fistulosa L. and Monarda punctata L. w...

The occurrence of candidiasis, including superficial infections, has recently increased dramatically, especially in immunocompromised patients. Their treatment is often ineffective due to the resistance of yeasts to antimycotics. Therefore, there is a need to search for new antifungals. The aim of this study was to determine the antifungal effect o...

Ziziphora species (Lamiaceae) have been used in traditional medicine as sedatives, antiseptics, carminatives, or expectorants. Despite their common applications in phytotherapy, there is still lack of evidence about the composition of their extracts and its impact on biological properties of the plants. The aim of this study was to evaluate the con...

Taxa belonging to the genus Miscanthus L. (silvergrass) are non-invasive plants that can grow on marginal land that are not suitable for food production. These species give high yields of lignocellulosic biomass, being a rich and renewable source of organic matter for the production of second-generation biofuels (ethanol) and for other practical pu...

Searching for novel antimicrobial agents is up to day topic for many group of researchers due to the fact that each year the number of bacterial strains resistant to currently used medicines increases. Special attention in the scientific literature among various groups of bioactive organic compounds is focused on the antimicrobial activity of hydra...

In our research, we used nicotinic acid as a starting compound, which was subjected to a series of condensation reactions with appropriate aldehydes. As a result of these reactions, we were able to obtain a series of twelve acylhydrazones, two of which showed promising activity against Gram-positive bacteria (MIC = 1.95–15.62 µg/mL), especially aga...

Antibiotic resistance is now a global problem, and the lack of effective antimicrobial agents for the treatment of diseases caused by resistant microbes is increasing. The 3-acetyl-2,5-disubstituted-1,3,4-oxadiazolines presented in this article may provide a good starting point for the development of potential new effective antimicrobial agents use...

Many of the essential oils obtained from medicinal plants possess proven antimicrobial activity and are suitable for medicinal purposes and applications in the food industry. The aim of the present work was the chemical analysis of 19 essential oils (EOs) from seven different Cymbopogon species (C. nardus, C. citratus, C winterianus, C. flexuosus,...

Introduction. Mouthwashes should include antimicrobial compounds to inhibit microorganism multiplication, thus the formation and development of dental plaque. Aim. The aim of the study was to evaluate the antibacterial and antifungal effectiveness of mouthwashes depending on their active ingredients. Material and methods. In the study, the effect o...

Recently, the occurrence of candidiasis has increased dramatically, especially in immunocompromised patients. Additionally, their treatment is often ineffective due to the resistance of yeasts to antimycotics. Therefore, there is a need to search for new antifungals. A series of nine newly synthesized thiazole derivatives containing the cyclopropan...

Candida spp. is the most prevalent cause of fungal infection worldwide, and their increasing resistance to anti-fungal agents, especially to azoles, has become problematic. The aim of this work was to establish the susceptibility to fluconazole, itraconazole, voriconazole and posaconazole of 50 clinical C. albicans isolates from hematooncological p...

There is a need to search for new antifungals, especially for the treatment of the invasive Candida infections, caused mainly by C. albicans. These infections are steadily increasing at an alarming rate, mostly among immunocompromised patients. The newly synthesized compounds (3a–3k) were characterized by physicochemical parameters and investigated...

This research describes the synthesis and in vitro antimicrobial activity study of a series of 2,4,6-trimethylbenzenesulfonyl hydrazones. Twenty-five hydrazones (2–26) were synthesized on the basis of condensation reaction. The in vitro bioactivity study confirmed the potential application of obtained derivatives as antimicrobial agents. Among the...

There is a need to search for new antifungals, especially for the treatment of the invasive Candida infections, caused mainly by C. albicans. These infections are steadily increasing at an alarming rate, mostly among immunocompromised patients. The newly synthesized compounds (3a-3k) were characterized by physico-chemical parameters and investigate...

Bacterial infections, especially those caused by strains resistant to commonly used antibiotics and chemotherapeutics, are still a current threat to public health. Therefore, the search for new molecules with potential antimicrobial activity is an important research goal. In this article, we present the synthesis and evaluation of the in vitro anti...

Extracts from the June collection of aerial parts of Helianthus salicifolius A. Dietr and Helianthus tuberosus L. were obtained using carbon dioxide supercritical fluid extraction with water as co-solvent. The antimicrobial effect in vitro of these extracts was then determined against reference species of bacteria, as well as against fungi (represe...

The aim of our study was the two-stage synthesis of 1,3,4-oxadiazole derivatives. The first step was the synthesis of hydrazide–hydrazones from 3-methyl-4-nitrobenzhydrazide and the corresponding substituted aromatic aldehydes. Then, the synthesized hydrazide–hydrazones were cyclized with acetic anhydride to obtain new 3-acetyl-2,3-disubstituted-1,...

Hygienic procedures in oral cavity, both teeth brushing and using mouthwashes, have an influence on the composition and quantity of oral microflora. The aim of the work was to evaluate the impact of regular use of selected mouthwash on the titer of Lactobacillus and Streptococcus bacteria and Candida yeast-like fungi in the saliva. The evaluation o...

The extracts from the aerial parts of Helianthus salicifolius A. Dietr and Helianthus tuberosus L. collected in June were obtained using carbon dioxide supercritical fluid extraction with water as co-solvent. The antimicrobial activity in vitro of these extracts were determined against the reference species of Gram-positive and Gram-negative bacter...

In this study 14 novel hydrazide-hydrazones of 5-bromo-2-iodobenzoic acid (3-16) were synthesized on the basis of condensation reaction. The chemical structure of obtained derivatives was established on the basis of spectral data (¹H NMR and ¹³C NMR) and the lipophilicity of synthesized molecules was determined with the use of RP-HPTLC chromatograp...

One of the strategies for seeking new biologically active substances is to modify compounds with potential biological activity. In this paper, 1,2,4-triazolin-5-thione derivative (3) was obtained in the cyclization reaction of appropriate thiosemicarbazide (2) as an organic ligand. The copper(II) complex, [CuCl2(H2O)2L2] (L=4-cyclohexyl-3-(nitrophe...

The aim of the present study was to determine the composition, antiradical and antimicrobial activity of fruits, leaves and roots of an underestimated species of barberry-Berberis iliensis-growing in Kazakhstan. Particular attention was paid to the determination of the composition of its extracts by high-performance liquid chromatography coupled wi...

The purpose of this research was to investigate the chemical composition of essential oils (EOs) from: Origanum vulgare L., Satureja hortensis L., Thymus serpyllum L. and Thymus vulgaris L. (Lamiaceae) cultivated in Poland, and to study their antifungal activity towards clinical isolates of oral Candida spp. The hydrodistilled essential oils were a...

Synthesis and investigation of antimicrobial activity of fifteen novel thiazoles containing cyclohexene moiety are presented. Among the derivatives, compounds 3a–3d, 3f, 3n, and 3o showed very strong activity against the reference Candida spp. strains with MIC = 0.015–3.91 µg/ml. The activity of these compounds is similar and even higher than the a...

In this research we synthesized and evaluated for in vitro antimicrobial activity a new series of hydrazide‐hydrazones obtained from 5‐nitrofuran‐2‐carboxylic acid. New compounds were identified and characterized by spectral methods (1H NMR and 13C NMR). All tested hydrazide‐hydrazones proved to be promising antimicrobial agents. Antimicrobial and...

Thirteen new 3‐acetyl‐2,5‐disubstituted‐1,3,4‐oxadiazoline derivatives were synthesized from corresponding hydrazide‐hydrazones of isonicotinic acid in the reaction with acetic anhydride. The obtained compounds were identified with the use of spectral methods (IR, 1H‐NMR, 13C‐NMR, MS). In vitro antimicrobial activity screening of synthesized compou...

Searching for new antibacterial agents is getting more and more challenging due to increasing drug resistance, which has become a major concern in the field of infection management. Our study presents synthesis and characterization by IR, UV, 1H-NMR and 13C-NMR spectra, a homogenous series of β-EWG functionalyzed β-nitrostyrenes, which could be can...

Synthesis and investigation of antifungal, anticonvulsant and anti-Toxoplasma gondii activities of ten novel (2-(cyclopropylmethylidene)hydrazinyl)thiazole 3a–3j are presented. Among the derivatives, compounds 3a–3d and 3f–3j possess very high activity against Candida spp. ATCC with MIC = 0.015–7.81 µg/ml. Compounds 3a–3d and 3f–3j possess also ver...

In this research we synthesized, identified and evaluated new hydrazide-hydrazones (1-3) and 1,3-thiazolidin-4-one derivatives (4-6) for in vitro and in vivo activity. New hydrazide-hydrazones (1-3) were obtained by the condensation reaction of 3-hydroxy-2-naphthoic acid hydrazide with appropriate aldehydes. Synthesized hydrazide-hydrazones (1-3) w...

Four new Pd(II) complexes are presented, [Pd(L1)(Cl)]K (1), [Pd(L1)(Cl)]Na (2), [Pd(L2)(Cl)]Na (3) and [Pd(L2)(H2O)] (4), where the ligands are derived from ortho-vanillin and either L-glutamic acid (L1) or L-tyrosine (L2). The complexes were characterized by X-ray diffraction studies, ESI-MS, IR and NMR spectroscopy and elemental analysis. Complex...

Synthesis and investigation of antifungal activity of ten novel thiazoles derived from tetrahydrothiophen-3-one are presented. Among the derivatives, (E/Z)-2-(2-(dihydrothiophen-3(2H)-ylidene)hydrazinyl)thiazoles 3a-3d and 3h had very strong activity against reference strains of Candida albicans and Candida krusei with MIC = 0.24–3.91 μg/ml. Their...

This article describes the synthesis and antimicrobial activity evaluation of new pipemidic acid derivatives. New compounds were obtained on the basis of Mannich reaction of 4,5-disubstituted 1,2,4-triazole-3-thiones with pipemidic acid. Antimicrobial tests revealed high antibacterial activity of obtained derivatives. Gram-negative rods belonging t...

In this study, we described the synthesis of the derivatives of thiosemicarbazide, dicarboximide, 1,2,4-triazole-5-thione and 4-oxo-1,3-thiazolidine. Two different dicarboxylic acid anhydrides reacted with 4-substituted-3-thiosemicarbazide, and derivatives of thiosemicarbazide and dicarboximide were obtained. Next, cyclization reaction of dicarboxi...

Fungal infections are an important medical problem in patients from different risk groups. The majority of these infections are caused by Candida spp., with over 50% due to C. albicans. The purpose of the study was to evaluate in vitro chlorhexidine effect on C. albicans colonizing the mouth and throat isolated from 5 population groups. The study m...

The aim of the work done was the qualitative and quantitative assessment of a polyphenolic fraction contained in methanolic extracts obtained from the flowering herbs of Marrubium incanum Desr., M. peregrinum L., M. thessalum Boiss. & Heldr., and M. candidissimum L. The samples containing phenolic acids, having undergone acid and alkaline hydrolysi...

This paper describes the synthesis, lipophilicity and in vitro antimicrobial assays of 15 new hydrazide-hydrazones of isonicotinic acid. New derivatives were obtained on the basis of the condensation reaction of isonicotinic acid hydrazide with different aromatic aldehydes. The chemical structure of synthesized compounds was confirmed by spectral m...

Synthesis and investigation of antimicrobial and anticonvulsant activity of eleven novel quinuclidinone-thiazole derivatives are presented. Among the derivatives, compounds 3a–3f showed a broad-spectrum antibiotic activity against all reference Gram-positive or Gram-negative bacteria and fungi. These compounds exhibited very strong or good bacteric...

New derivatives of 2,5-dimercapto-1,3,4-thiadiazole substituted both at one or two exocyclic sulfur atoms with a series of aroyl or ethoxycarbonyl groups were synthesized in reactions of 2,5-dimercapto-1,3,4-thiadiazole salts with appropriate acid chlorides or ethyl chloroformate in mild conditions. The products were characterized by spectroscopy (...

In this research we synthesized and tested for in vitro antimicrobial activity 21 nitrofurazone analogues. The compounds we obtained were identified on the basis of ¹H NMR and ¹³C NMR spectroscopy. The in vitro screening of antimicrobial properties of synthesized compounds revealed a wide spectrum of antimicrobial activity. Compounds 28, 29, 32-43,...

The main aim of this research was the synthesis, spectral identification and in vitro antimicrobial evaluation of new hydrazides and hydrazide-hydrazones of 2,3-dihalogen substituted propionic acids. New hydrazides were obtained by the substitution reaction of appropriate ethyl esters of 2,3-dihalogen substituted propionic acids with hydrazine hydr...

Reaction of phenoxyacetic acid hydrazide with isocyanate was used to the synthesis of new semicarbazide derivatives. Cyclization of these compounds in a 2% aqueous solution of sodium hydroxide led to formation of 1,2,4-triazole-3-one. The chemical structure of synthesized compounds was confirmed by elemental analysis and spectroscopic methods (1H a...

This study presents the synthesis and antimicrobial properties of new Mannich bases of 1,2,4-triazole-3-thiones. New compounds were prepared by the reaction of 4,5-disubstituted-1,2,4-triazole-3-thiones with formaldehyde and various amines. The structures of the prepared compounds were established on the basis of spectral methods (¹H NMR, ¹³C NMR)....

In this work the phenolic acids in crude methanol extracts from the flowering herbs of Carluus acanthoides L. were identified. The samples containing free phenolic acids and those released after acid and alka- line hydrolyses were investigated by 2D TLC on cellulose. After purification by SPE, samples were also analyzed by RP-HPLC. Chlorogenic, pro...

Drug resistance has become a major concern in the field of infection management, therefore searching for new antibacterial agents is getting more and more challenging. Our study presents an optimized and eco-friendly synthesis scheme for a panel of nitroalkenes bearing various functional groups in the aromatic moiety and bromine or cyano substituen...

In this paper, 14 new nalidixic acid–1,3-thiazolidin-4-one hybrid compounds (17–30) were synthesized by the cyclization reaction of corresponding N-substituted nalidixic acid hydrazones (3–16) with mercaptoacetic acid in the presence of 1,4-dioxane. The structures of the obtained compounds were confirmed by means of ¹H NMR and ¹³C NMR spectroscopy....

The aim of present study was to determine and to compare the chemical composition of six Impatiens species. For this purpose, UHPLC-DAD-MS3 analyses and spectrophotometric assays were used. The results revealed the presence of nineteen constituents comprising phenolic acids and flavonoids. The total phenolic content in the samples ranged from 8.8 t...

The title compounds are prepared by cyclization reaction of a variety of N-substituted carboxylic acid hydrazides with mercaptoacetic acid.

In this study 30 hydrazide-hydrazones of phenylacetic (3-10) and hydroxyacetic acid (11-32) were synthesized by the condensation reaction of appropriate 2-substituted acetic acid hydrazide with different aromatic aldehydes. The obtained compounds were characterized by spectral data and evaluated in vitro for their potential antimicrobial activities...

Studies of the stability of a ligand derived from L-glutamic acid and ortho-vanillin and its new [Cu4] complex are presented. The [Cu4] complex contains a heterocubane [CuII4O4] core and pendant carboxylic groups increasing its solubility in water, also under basic conditions. The stability of the complex in different solvents is confirmed with ESI...

Abstract In this paper, we describe twelve 3-hydroxy-2-naphthoic hydrazide derivatives (six thiosemicarbazides and six 1,2,4-triazole-3-thiones) which we have synthesized. Thiosemicarbazide derivatives were prepared by condensation reaction of 3-hydroxy-2-naphthoic hydrazide with appropriate isothiocyanates. The 1,2,4-triazole-3-thiones were synthe...

Synthesis and investigation of antimicrobial activity of 22 novel thiazoles and selenazoles derived from dihydro-2H-thiopyran-4(3H)-one are presented. Additionally, anticonvulsant activity of six derivatives is examinated. Among the derivatives, compounds 4a–f, 4i, 4k, 4 l, 4n, 4o–s and 4v have very strong activity against Candida spp. with MIC = 1...

A series of 28 hydrazide-hydrazones of 3-methoxybenzoic and 4-tert-butylbenzoic acid were synthesized and screened in vitro against the panel of reference strains of bacteria and fungi with the use of the broth microdilution method according to EUCAST and CLSI guidelines. Five of the synthesized compounds were found to exhibit high bacteriostatic o...

Background Haemophili are representative microbiota of the upper respiratory tract. The aim of this study was to assess the effects of perioperative antimicrobial prophylaxis and/or postoperative treatment on Haemophilus parainfluenzae prevalence, and antimicrobial sensitivity in short-term hospitalized patients with lung cancer who underwent surge...

Background Haemophilus species are the most common microbiota in humans. The aim of this paper was to investigate Haemophilus spp., mainly H. parainfluenzae prevalence, in the upper respiratory tract of chronic hepatitis C (CHC-positive) patients with or without therapy using pegylated interferon alfa and ribavirin. Material/Methods We collected 4...

Background: Synthesis, characterization and investigation of antibacterial activity of ten novel Schiff base derivatives of 4-formylbenzoic acid is presented. Their structures were determined using 1H and 13CNMR, EI(+)-MS and elemental analyses. Additionally, DFT calculations of interaction energies in complexes of the novel drugs and DNA bases ar...

Synthesis and investigation of antimicrobial activities of novel thiazoles and selenazoles is presented. Their structures were determined using NMR, FAB(+)-MS, HRMS and elemental analyses. To support the experiment, theoretical calculations of the 1H NMR shifts were carried out for representative systems within the DFT B3LYP/6-311++G** approximatio...

An analysis of the fractions of free acids and phenolic acids liberated by way of hydrolysis in the rhizomes and herbaceous tissues of Sanguisorba officinalis L. was conducted through utilizing the 2D-TLC method. Fifteen phenolic acids were identified. Our work showed that ellagic, protocatechuic, gentisic, p-hydroxybenzoic, syringic, vannilic and...

A series of nalidixic acid-based hydrazones have been synthesized and evaluated for their in vitro antimicrobial activity using the broth microdilution method against a panel of reference strains of microorganisms, including Gram-positive bacteria, Gram-negative bacteria, and fungi belonging to yeasts Candida spp. and molds Aspergillus spp., Penici...

The aim of this study was to investigate phenolic acids and flavonoids in methanolic, dichloromethane, acetone and ethyl acetate extracts and fractions from inflorescences of Cirsium canum (L.). RP-HPLC analysis enabled identification of the following: chlorogenic acid, caffeic acid, p-coumaric acid, protocatechuic acid, p-hydroxybenzoic acid, vani...

Synthesis, characterization and investigation of antimicrobial activity of 11 novel adamantanyl-thiazoles are presented. Their structures were determined using 1H and 13C NMR, EI(+)-MS, HRMS, and elemental analyses. Among the derivatives, compound 3c showed very strong activity, especially against Candida albicans ATCC 10231 and Candida parapsilosi...

This study presents the design, synthesis, spectral analysis, and in vitro antimicrobial evaluation of a new series of furan/thiophene-1,3-benzothiazin-4-one hybrids (17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32). New compounds were obtained by cyclization reaction of N-substituted furan/thiophene-2-carboxamide derivatives (1, 2,...

In this article, a new series of 2,3-disubstituted-1,3-thiazolidin-4-one derivatives have been designed, synthesized, and evaluated as antimicrobial agents. New compounds were prepared by the cyclization reaction of N-substituted carboxylic acid hydrazide derivatives with mercaptoacetic acid. The structures of the obtained compounds were confirmed...

Synthesis, characterization, and investigation of antimicrobial activities of nine novel imidazolylthiazoles are presented. Their structures were determined using 1H NMR, 13C NMR, and elemental analysis. Compounds 3e and 3i show very strong or strong bacteriostatic or bactericidal activity [minimal bactericidal concentration/minimal inhibitory conc...

Infections caused by non-albicans Candida spp. are an important medical problem in people from risk groups, e.g. hematooncological patients. The aim of this paper was to analyse the in vitro activity of micafungin against 30 clinical isolates of non-albicans Candida spp. (C. glabrata, C. famata, C. tropicalis, C. inconspicua, C. lusitaniae, C. para...