Anja Kunze

Anja Kunze
Montana State University | MSU · Department of Electrical & Computer Engineering

Ph.D. - www.anyakunze.com

About

34
Publications
2,362
Reads
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589
Citations
Additional affiliations
August 2016 - present
Montana State University
Position
  • Professor (Assistant)
July 2015 - June 2016
University of California, Los Angeles
Position
  • Assistant Adjunct Professor
April 2012 - June 2012
University of California, Los Angeles
Position
  • PostDoc Position

Publications

Publications (34)
Article
Tangential curvatures are a key geometric feature of tissue folds in the human cerebral cortex. In the brain, these smoother and firmer bends are called gyri and sulci and form distinctive curved tissue patterns imposing a mechanical stimulus on neuronal networks. This stimulus is hypothesized to be essential for proper brain cell function but lack...
Article
Full-text available
Calcium fluorometry is critical to determine cell homeostasis or to reveal communication patterns in neuronal networks. Recently, characterizing calcium signalling in neurons related to interactions with nanomaterials has become of interest due to its therapeutic potential. However, imaging of neuronal cell activity under stable physiological condi...
Article
Full-text available
Cellular processes like membrane deformation, cell migration, and transport of organelles are sensitive to mechanical forces. Technically, these cellular processes can be manipulated through operating forces at a spatial precision in the range of nanometers up to a few micrometers through chaperoning force-mediating nanoparticles in electrical, mag...
Article
Vesicle transport is a major underlying mechanism of cell communication. Inhibiting vesicle transport in brain cells results in blockage of neuronal signals, even in intact neuronal networks. Modulating intracellular vesicle transport can have a huge impact on the development of new neurotherapeutic concepts, but only if we can specifically interfe...
Article
Despite increasing use of nanotechnology in neuroscience, the characterization of interactions between magnetic nanoparticles (MNPs) and primary cortical neural networks remains underdeveloped. In particular, how the age of primary neural networks affects MNP uptake and endocytosis is critical when considering MNP-based therapies for age-related di...
Article
Cellular processes like signaling, growth and network formation in neurons are highly linked to intracellular vesicle motion. To manipulate signaling from inside the cell, we utilize functionalized superparamagnetic nanoparticles (fMNPs) combined with amplified magnetic field gradients to direct and alter vesicle transport based on magnetic forces....
Article
Nano-magnetic force stimulation with ferromagnetic nanoparticles was found to trigger calcium influx in cortical neural networks without observable cytotoxicity. Stimulated neural networks showed an average of 20% increment in calcium fluorescence signals and a heightened frequency in calcium spiking. These effects were also confined spatially to a...
Article
Intra- and extracellular signaling play critical roles in cell polarity, ultimately leading to the development of functional cell-cell connections, tissues and organs. In the brain, pathologically-oriented neurons are often the cause for disordered circuits, severely impacting motor function, perception and memory. Aside from control through gene e...
Article
Compartmentalized microfl uidic devices are designed to engineer the cellular environment for cell cultures. The practical use of the compartmentalized chambers can be expanded to induce co-pathological cell cultures, where one cell population expresses a specifi c disease state, while being in direct-cell or metabolic contact to a second or third...
Article
We highlight recent progress in applying micro- and nanotechnology enabled cell separations to life sciences and clinical use. Microfluidic systems operate on a scale that matches that of cells (10-100 μm) and therefore allow interfacing and separations that are sensitive at this scale. Given the corresponding dimensions, it is not surprising that...
Article
A process to surface pattern polydimethylsiloxane (PDMS) with ferromagnetic structures of varying sizes (micrometer to millimeter) and thicknesses (>70 μm) is developed. Their flexibility and magnetic reach are utilized to confer dynamic, additive properties to a variety of substrates, such as coverslips and Eppendorf tubes. It is found that these...
Article
Microfabricated systems and microfluidic tools are well-suited to interface with cells because of the matching length scales. In this issue, we highlight three recent papers in which unique tools were used to control or measure cell migration. Cell migration is a key biological process involved in normal physiology (e.g. in embryonic development, i...
Article
In this issue we highlight recent microtechnology-enabled approaches to control the physical and biomolecular environment around cells: (1) developing micropatterned surfaces to quantify cell affinity choices between two adhesive patterns, (2) controlling topographical cues to align cells and improve reprogramming to a pluripotent state, and (3) co...
Article
Micro-scale biological tools that have allowed probing of individual cells - from the genetic, to proteomic, to phenotypic level - have revealed important contributions of single cells to direct normal and diseased body processes. In analyzing single cells, sample heterogeneity between and within specific cell types drives the need for high-through...
Article
Amyotrophic lateral sclerosis (ALS) is the most common motor neuron disease. ALS is believed to be a non-cell autonomous condition, as other cell types, including astrocytes, have been implicated in disease pathogenesis. Hence, to facilitate the development of therapeutics against ALS, it is crucial to better understand the interactions between ast...
Article
Mechanical forces have long been studied in relation to stem cell differentiation or cell migration, however, their significance during brain and neural circuit formation are poorly understood. We present a magnetic gradient platform that allows investigation of small scale forces acting on arrays of neurons in parallel. The approach draws magnetic...
Article
A major problem in traditional cell culture methods, such as Petri dishes and culture flasks, is the very simplified artificial environment around the cells. Traditional cell culture methods lack features of the native cell niche, such as gradients and cell organization. This lack probably explains why pharmaceutics against the neurodegenerative Al...
Data
Morphological evaluation through immunostaining. Chosen immunostainings are explained in detail and non specific binding issues in the hydrogel are discussed. (DOC)
Data
Evaluation of the cell response on the NGF/B27 gradient based on synaptic density. This file gives further details on evaluating spatial synapse distribution through determining local synaptic densities. (DOC)
Data
Design and fabrication of the microfluidic based cell culture device. This file gives further details on the microfluidic design and its fabrication steps. (DOC)
Data
Chemical gradient characterization and modeling. Details on gradient measurements and modelling are presented. (DOC)
Data
Synapse distribution increases with a higher gradient slope. Additional results that show the increased spatial synapse distribution through increased gradient slope. (DOC)
Data
Evaluation of the cell response on the NGF/B27 gradient based on synapse formation. This file gives details how spatial synapse distribution was evaluated based on spatial fluorescence intensity measurements. (DOC)
Article
Full-text available
Native functional brain circuits show different numbers of synapses (synaptic densities) in the cerebral cortex. Until now, different synaptic densities could not be studied in vitro using current cell culture methods for primary neurons. Herein, we present a novel microfluidic based cell culture method that combines 3D micropatterning of hydrogel...
Article
This communication presents a novel experimental model for Alzheimer studies, where connected primary neurons were set into subtend, co-pathological states. Cortical neurons were cultured in two separated cell compartments in a microfluidic device. A neurite network was generated in a main channel through the neurite outgrowth from both cell compar...
Article
Cortical neurons, in their native state, are organized in six different cell layers; and the thickness of the cell layer ranges from 0.12 mm to 0.4 mm. The structure of cell layers plays an important role in neurodegenerative diseases or corticogenesis. We developed a 3D microfluidic device for creating physiologically realistic, micrometer scaled...
Article
Full-text available
By enhancing the environment of micro electrode arrays with microfluidic channels we cultured primary neuronal cells in patterned two- and three dimensional structures. Alternatively, the same microfluidic channels can be used to generate a local chemical stimulation over a two-dimensional neuronal cell culture. We demonstrate that a significantly...
Article
Full-text available
Neuronal cell culture models in vitro are often restricted to 2D surfaces. Engineering the complexity of the neuronal microenvironment in microfluidic systems can help to generate more tissue like cultures. We have developed a new neuronal cell culturing system based on a microfluidic device that can culture primary neurons in a 3D patterned hydro-...
Article
Full-text available
We present a simple and easy to handle PDMS microfluidic device for neuronal cell culture studies in three-dimensional hydrogel scaffolds. The hydrogel is structured in parallel layers to reconstruct cell layers close to the natural environment. Dissociated cortical neurons of embryonic rats have been cultured in 0.5% w/v agarose including 0.2% w/v...
Article
Controlling alginate gel formation by diffusion of Ca ions through a filter barrier, a layer-by-layer deposition technique with resolution on the size scale of a single cell is presented. It gives the possibility of exposing cells under biocompatible conditions to microheterogeneous three-dimensional environments, mimicking the layered structure of...
Article
We present a channel geometry that allows for clean switching between different inlets of a microchip without any contamination of the inlets or the downstream flow. We drive this virtual valve with a pneumatic pressure setup that minimizes disturbance of the downstream flow during the switching procedure by simultaneous variation of the pressures...
Article
Solvent viscosity was found to be a predominant parameter to control free-radical surface graft polymerization. Poly(ethylene-alt-tetrafluoroethylene) foils were lithographically exposed to 92 eV photons in order to create patterns of radicals at their surface, which react with the oxygen of the ambient air to produce peroxides. Glycidyl methacryla...

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