Anis Ben Hsouna

Higher Institute of Applied Sciences and Technology of Mahdia

Citation: Ben Akacha, B.; Garzoli, S.; Ben Saad, R.; Brini, F.; Mnif, W.; Kačániová, M.; Ben Hsouna, A. Biopreservative Effect of the Tunisian Halophyte Lobularia maritima Flavonoid Fraction, Used Alone and in Combination with Linalool in Stored Minced Beef Meat. Metabolites 2023, 13, 371. https://doi.

Citrus fruits occupy an important position in the context of the fruit trade, considering that both fresh fruits and processed products are produced on a large scale. Citrus fruits are recognized as an essential component of the human diet, thanks to their high content of beneficial nutrients such as vitamins, minerals, terpenes, flavonoids, coumar...

Cold stress is a key environmental factor affecting plant growth and development, crop productivity, and geographic distribution. Thioredoxins (Trxs) are small proteins that are ubiquitously expressed in all organisms and implicated in several cellular processes, including redox reactions. However, their role in the regulation of cold stress in the...

Rapid global modernization, urbanization, and industrialization have accelerated the release of heavy metals, causing soil pollution. These highly noxious environmental pollutants induce oxidative stress in plants via stimulation of the production of reactive oxygen species (ROS). Thioredoxin (Trxs) is a highly conserved disulfide reductase that pl...

In this study, a new heteropolysaccharide extracted from Lobularia maritima (L.) Desv. (LmPS), a halophyte harvested in Tunisia, was evaluated as an antioxidant and antibacterial additive in the bio-preservation of raw minced meat. For antibacterial testing, Gram-positive bacteria such as Staphylococcus aureus ATCC and Listeria monocytogenes ATCC 1...

Family members within the plant-specific gibberellic acid-stimulated Arabidopsis (GASA) gene serve a crucial role in plant growth and development, particularly in flower induction and seed development. Through a genome-wide analysis of Triticum turgidum ssp. Durum (durum wheat), we identified 19 GASA genes, designated as TdGASA1‒19. Moreover, the c...

This study aims to investigate the phytochemical composition of various extracts (hexane, dichloromethane, ethyl acetate, and aqueous) of L. maritima. The extracts were tested for their anti-inflammatory and antimicrobial properties and to demonstrate the potential use of L. maritima extract, as a source of natural antioxidants, to preserve meat qu...

Halophytes are plant species widely distributed in saline habitats, such as beaches, postindustrial wastelands, irrigated lands, salt flats, and others. Excessive salt level, known to limit plant growth, is not harmful to halophytes, which have developed a variety of defense mechanisms allowing them to colonize harsh environments. Plants under stre...

This study was directed towards the investigation of the chemical composition and antimicrobial properties of the essential oil of Tunisian halophyte Lobularia maritime (LmEO). The antibacterial effects against major food-borne pathogenic and food spoilage bacteria were tested using the well diffusion method, followed by the determination of the mi...

The present study aimed to evaluate deer meat microbiological quality when treated with essential oil (EO) from Litsea cubeba (dissolved in rapeseed oil at concentrations 0.5 and 1%), in combination with vacuum packaging during 20 days of storage of meat at 4 °C. Total viable counts (TVC), coliforms bacteria (CB), lactic acid bacteria (LAB) and Pse...

Bioactivities of polysaccharides derived from halophyte plants have gained attention in recent years. The use of biostimulants in agriculture is an innovative method of dealing with environmental stressors affecting plant growth and development. Here, we investigated the use of natural polysaccharides derived from the halophyte plant Lobularia mari...

Thiamine (TA), also known as vitamin B1, is an essential amino acid derived from food sources for normal body function. TA is thought to have antioxidant, antimicrobial, and anti-inflammatory effects in addition to its nutritional benefits. The degree to which a number of microorganisms implicated in food rotting are sensitive to increasing concent...

The current study explorated the hepatoprotective and immunomodulatory effects of Linalool (Lin) against carbon tetrachloride (CCl4)‐induced toxicity in mice. Four study groups (n = 8 each) were used: (1) a negative control group; (2) a toxicity control group (single dose of CCl4 administered at day 14 as 1ml/kg of CCL4 in 1% olive oil. Intraperito...

Thioredoxins (Trxs) are multi-functional redox proteins characterized by a conserved redox-active CGPC site. In plants, Trxs are small antioxidant proteins encoded by a multigene family and regulate several processes during development and growth. Consequently, Thrxs are attractive candidates for transgenic plant improvement to overcome the abnorma...

Abstract The aim of the present study was to investigate the extraction and the characterization of a novel heteropolysaccharide from Tunisian halophyte Lobularia maritima (LmPS). We were also interested in its antioxidant, anti‐inflammatory, and hepatoprotective effects on carbon tetrachloride (CCl4)‐induced liver injury in rats. LmPS physicochemi...

TdAnn6 is a gene encoding an annexin protein in durum wheat (Triticum durum). The function of TdAnn6 in plant response to stress is not yet clearly understood. Here, we isolated TdAnn6 and characterized it in genetically modified Arabidopsis thaliana. Expressing TdAnn6 in Arabidopsis coincided with an improvement in stress tolerance at germination...

Lipid transfer proteins (LTP) are members of the family of pathogenesis-related proteins (PR-14) that play a key role in plant defense mechanisms. In this study, a novel gene TdLTP4 encoding an antifungal protein from wheat (cv. Om Rabiaa) was cloned, overexpressed in Escherichia coli BL-21 (DE3) and enriched using ammonium sulfate fractionation. T...

Halophyte Lobularia maritima LmSAP encodes an A20AN1 zinc-finger stress-associated protein which expression is up-regulated by abiotic stresses and heavy metals in transgenic tobacco. To deepen our understanding of LmSAP function, we isolated a 1,147 bp genomic fragment upstream of LmSAP coding sequence designated as PrLmSAP. In silico analyses of...

Soil salinity is an abiotic stress that reduces agricultural productivity. For decades, halophytes have been studied to elucidate the physiological and biochemical processes involved in alleviating cellular ionic imbalance and conferring salt tolerance. Recently, several interesting genes with proven influence on salt tolerance were isolated from t...

Stress-associated proteins (SAPs) are favorable targets to improve stress tolerance in plants, owing to their roles in developmental processes and stress responses. However, the role of SAPs and the molecular mechanisms by which they regulate plant stress responses remain poorly understood. Previously, it was reported that LmSAP expression was upre...

Stress-associated proteins (SAPs), such as A20/AN1 zinc-finger domain-containing proteins, have emerged as a novel class of proteins involved in abiotic stress signaling, and they are important candidates for preventing the loss of yield caused by exposure to environmental stresses. In a previous report, it was found that the ectopic-expression of...

Lobularia maritima (Alyssum maritimum, Brassicaceae), commonly known as sweet alyssum, is an annual ornamental halophyte widely spread along the Tunisian seashore. Lobularia maritima leaf ethanol extract was tested in an experimental model of hepatotoxicity induced by carbon tetrachloride (CCl4). L. maritima extract was found to possess in vitro an...

The present study was undertaken to investigate the expression, purification and biological activities of a novel Triticum durum Annexin 12 protein (TdAnn12). The findings indicated that the molecular weight of the purified TdAnn12 was estimated to 35 kDa. The purified TdAnn12 protein was modulated by, Methyl-jasmonate, and ethephon treatments. The...

Crop productivity depends heavily on several biotic and abiotic factors. Plant annexins are a multigene family of calcium-dependent phospholipid-binding proteins that function in response to environmental stresses and signaling during growth and development of plants. We recently isolated and characterized a Triticum durum annexin, called TdANN12,...

The present study evaluates the chemical profiling of the essential oil of a halophyte, L. maritima (LmEO), and its protective potential against CCl4-induced oxidative stress in rats. Forty compounds have been identified in LmEO. The major components are α-pinene (3.51%), benzyl alcohol (8.65%), linalool (22.43%), pulegone (3.33%), 1-phenyl butanon...

Plant annexins are proteins with multiple functions and roles in plant development and responses to abiotic stresses.Wereport here the functional analysis of the TdAnn12 annexin protein isolated from Triticum durum Desf.Wehave previously shown that TdAnn12 expression is highly induced by different abiotic stresses. In the present study, to investig...

This study was undertaken to determine the antibacterial efficacy of the essential oil (EO) of peppermint (Mentha piperita L.), in vitro and in vivo, against the phytopathogenic bacteria Agrobacterium tumefaciens (A. tumefaciens). The EO composition of M. piperita L. was investigated by Gas chromatography-mass spectrometry (GC/MS) with 26 identifie...

Myrtus communis L. (Myrtle) is one of the most important aromatic and medicinal species from the Myrtaceae family. It is traditionally used as antiseptic, disinfectant drug and hypoglycemic agent. The aim of our study was to evaluate the protective effect of Myrtus communis essential oil (McEO) on CCl4-induced hepatotoxicity in rat. Thirty two adul...

The present study aimed (1) to investigate the chemical composition as well as the anti‐inflammatory properties and in vitro antioxidant activity of Citrus aurantium peel essential oil (p EOCa ) and (2) to evaluate its potential effect in vivo. The main results showed that the major components of p EOCa are Limonene and Linalool. Additionally, DPPH...

The objective of this study is to explore the preventive effects of ethyl acetate fraction from Citrus aur-antium leaf extract (EACA), associated with its phytochemical content, against the toxic impacts of acute exposure to carbon tetrachloride (CCl 4) in the liver of adult rats. HPLC analysis of ethyl acetate fraction from extract C. aurantium re...

Background Mentha piperita L. is a flowering plant belonging to the Lamiaceae family. Mentha plants constitute one of the main valuable sources of essential oil used in foods and for medicinal purposes. Methods The present study aimed to investigate the composition and in vitro antioxidant activity of Mentha piperita leaf essential oil (MpEO). A si...

Background Lemon (Citrus limon) is a flowing plant belonging to the Rutaceae family. Citrus plants constitute one of the main valuable sources of essential oil used in foods and medicinal purposes. Methods In this study, we assessed chemical composition, antioxidant and antimicrobial activities of C. limon essential oil (ClEO) with its preservativ...

A straightforward method has been developed for the synthesis of 1,2,4-triazol-3-one 3 and 1,2,4-triazoles 6a, 6b, 6c, 6d starting from N1-substituted-N1-tosylhydrazonates 2 and hydrazine monohydrate. This methodology affords a number of 1,2,4-triazol-3-one 3 and 1,2,4-triazoles 6a, 6b, 6c, 6d in reasonable yields. The structures of all new compoun...

The aims of this study were to assess the antimicrobial efficacy of the leaf essential oil and the leaf extracts of R. montana against Botrytis cinerea, Fusarium oxysporum, Verticillium dahliae, Aspergillus oryzae and Fusarium solani. The oil (1.000 µg/disk) and the extracts (1.500 µg/disk) revealed a remarkable antifungal effect against the tested...

Myrtus communis is a typical plant of the Mediterranean area, which is mainly used as animal and human food and, in folk medicine, for treating some disorders. In the present study, we evaluated in vitro antibacterial and antifungal properties of the essential oils of Myrtus communis (McEO), as well as its phytochemical composition. The GC/MS analy...

Artemisia phaeolepis, a perennial herb with a strong volatile odor, grows on the grasslands of Mediterranean region. Essential oil obtained from Artemisia phaeolepis was analyzed by gas chromatography-flame ionization detection and gas chromatography-mass spectrometry. A total of 79 components representing 98.19% of the total oil were identified, a...

This study aimed to investigate the antioxidant properties of different fractions obtained from the fruits of Lawsonia inermis, a widely used medicinal plant, against carbon tetrachloride (CCl4)-induced oxidative stress in rat liver. The results show that several fractions obtained from L. inermis fruits possessed important antioxidant activity. Am...

The preparation of novel metal-free phthalocyanines and metallophthalocyanine complexes 6 and 7 (MPcs, M = Co, Zn, Cu and Mn), with four peripheral 6-hydroxy-4-methylcoumarin and 6-hydroxycoumarin substituents, were prepared by cyclotetramerization of compounds 4 and 5 with the corresponding metal salts (Zn(OAc)(2)center dot 2H(2)O, Co(OAc)(2)cente...

Citrus aurantium L. essential oil is commonly used as a flavouring agent. In the present study, the essential oil of fresh Citrus aurantium L. (CaEO) flowers cultivated in North East of Tunisia (Nabeul) was analyzed by GC-FID and GC-MS. 33 compounds were identified, representing 99% of the total oil. Limonene (27.5%) was the main component followed...

The crude methanol extract of Citrullus colocynthis fruit and Emex spinosa leaves were examined for antimicrobial and antioxidant potentialities. The phytochemical analysis revealed presence of some bioactive principles, such as alkaloids, flavonoids and anthraquinones for E. spinosa and saponin, flavonoids, terpenoids and alkaloids for C. colocynt...

In the present study, we evaluated in vitro antimicrobial and antioxidant properties of Thymelaea hirsuta extracts as well as its phytochemical composition. Total phenolic and flavonoid contents were highest in acetone and ethyl acetate extracts respectively. The different extracts were tested against a panel of microorganisms, food-borne bacteria...

Background Pelargonium graveolens (P. graveolens) L. is an aromatic and medicinal plant belonging to the geraniacea family. Results The chemical compositions of the essential oil as well as the in vitro antimicrobial activities were investigated. The GC-MS analysis of the essential oil revealed 42 compounds. Linallol L, Citronellol, Geraniol, 6-Oc...

Fluorescent Pseudomonas spp., isolated from tomato and pepper plants rhizosphere soil, was evaluated in vitro as a potential antagonist of fungal pathogens. Pseudomonas strains were tested against the causal agents of tomatoes damping-off (Sclerotinia sclerotiorum), root rot (Fusarium solani), and causal agents of stem canker and leaf blight (Alter...

The antimicrobial activity of crude methanolic extract of Ceratonia siliqua (Carob) extracted from pods was tested on 13 different references and food isolated bacterial pathogens (8 Gram positive and 5 Gram negative) and 8 different references and food isolated fungal pathogens. Antimicrobial activities of the pods of C. siliqua were comparable to...

We present a new method for the synthesis of bis-triazolones 3 , which result from the processing of novel bis-amidrazones 2 with ethyl chloroformate. The bis-amidrazones 2 are prepared by the reaction of the N 1 -tosylhydrazonates 1 with two equivalents of aliphatic diamine. The structures of the new products were determined through IR, 1H and 13C...

Ceratonia siliqua is a typical Mediterranean plant, mainly used in food and Tunisian traditional folk medicine. Among the tested extracts, the ethyl acetate fraction (EACs) exhibited the highest total phenolic and flavonoids content. The antioxidant activity in vitro systems showed a more significant potent free radical scavenging activity of this...

Methanol is primarily metabolized by oxidation to formaldehyde and then to formate. These processes are accompanied by formation of superoxide anion and hydrogen peroxide. This article reports data on the effect of methanol-induced oxidative damage in experimental rats and the role of aqueous extract of Opuntia vulgaris fruit extract (OE) to counte...

The antioxidant potential of different fractions of Lawsonia inermis (Lythraceae) was investigated. The n-butanolic fraction showed the highest yield of extraction; it also exhibited a strong antioxidant activity in the DPPH assay and a potent capacity in preventing linoleic acid oxidation. Five phenolic glycosides were identified in this fraction....