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Introduction
PhD scholar researching the role of mitochondria and ER in ischemic stroke. My work focuses on organelle crosstalk, mitochondrial dynamics, and ER stress in post-stroke neurodegeneration. I use high-resolution respirometry, Western blotting, RT-PCR, immunofluorescence, biochemical assays, and in vitro assays to explore related molecular pathways and identify potential therapeutic targets.
Current institution
Publications
Publications (5)
Background
Pregnancy may be a risk factor for stroke in females. Stroke in pregnancy influences mitochondrial dynamics as well as the inflammatory responses in mothers. However, limited studies are available that report any epigenetic changes in the offspring following a stroke in mothers. In the present study we investigate the effect of stroke in...
Stroke is one of the major causes of mortality and long-term disability worldwide. Chronic-kidney-disease (CKD) is a condition where patients have shown increased vulnerability to stroke with poor functional and cognitive outcomes. Impaired cerebral autoregulation in CKD patients may impose a high risk of stroke. To date, the mechanism of worsened...
The progressive brain damage following ischemic stroke is primarily due to oxidative stress and activation of inflammatory pathways. Post-stroke neurodegeneration can lead to the loss of neurons and glial cells, including oligodendrocytes, contributing to demyelination. Following ischemic stroke, reperfusion results in increased intracellular calci...
Despite rapid advances in stroke management and rehabilitation therapy, no effective treatment is available for the later recovery phase following stroke. Therefore, complementary and alternative medicine system (CAM) has emerged as promising adjunct therapy for stroke management. CAM has its own cultural and philosophical aspects with different so...
Background
Conventional post-stroke edema management strategies are limitedly successful as in multiple cases of hemorrhagic transformation is being reported. Clinically, acute-ischemic-stroke (AIS) intervention by endovascular mesenchymal stem cells (MSCs) have shown benefits by altering various signaling pathways. Our previous studies have report...
Questions
Question (1)
whenever I take cryostat sections of rat brain, many holes are distorting the normal morphology(image attached). what may be the reason?
protocol which we follow:
* Perfuse the brain in PBS and then store in 4% PFA overnight (4 deg).
*Transfer and keep the brain in 15% sucrose until it sinks(4 deg).
*Transfer and keep the brain in 30% sucrose until it sinks(4 deg).
*Take out the brain and wipe the excess sucrose using tissue paper, and keep it in cryostat set to -20 °c.
*Embed the tissue using OCT media on a metal chuck by covering the tissue layer by layer inside the cryostat.
*Start sectioning.
