Aniket K Gade

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• PhD
• Associate Professor at Institute of Chemical Technology

Airborne pathogens, such as influenza and SARS‐CoV‐2, pose significant health risks. While traditional diagnostic methods have limitations, optical biosensors offer a promising solution due to their rapid, sensitive, and cost‐effective nature. This review focuses on the application of optical biosensors, including colorimetry, surface plasmon reson...

Extremophiles are organisms that can survive in what humans would consider to be ‘extreme’ environments. For several factors, conventional techniques are not suitable in situations with significant temperature and pH fluctuations, excessive salt, and so on. Extremophiles, namely, psychrophiles, acidophiles, thermophiles, and halophiles are environm...

Various Phytophthora species have long been reported to cause different citrus diseases, including gummosis, root rot, fruit brown rot, and citrus decline worldwide, including in India. Among these, Phytophthora nicotianae has gained considerable attention due to its ability to cause citrus gummosis, leading to significant yield losses (approximate...

Background: Phytochemicals, natural compounds derived from plants, have garnered significant attention for their potential health benefits and therapeutic properties. The burgeoning interest in leveraging phytochemicals for drug discovery underscores the necessity for comprehensive and reliable databases to store and analyze their structural and ac...

Global crop protection and food security have become critical issues to achieve the ‘Zero Hunger’ goal in recent years, as significant crop damage is primarily caused by biotic factors. Applying nanoparticles in agriculture could enhance crop yield. Nano-silver, or AgNPs, have colossal importance in many fields like biomedical, agriculture, and the...

Resistance against fungicides or antibiotics in plant pathogens is nowadays a greater challenge to the scientific community, as most of the pathogenic strains survive unless treated with anti-agents. Therefore, there is a need to develop sustainable approaches that could manage the diseases in plants. Silver nanoparticles (AgNPs) have received atte...

Background: Diosgenin, an essential sapogenin steroid with significant biological implications, is composed of a hydrophilic sugar moiety intricately linked to a hydrophobic steroid aglycone. While the antiviral properties of diosgenin against numerous RNA viruses have been extensively documented, its potential in combating Human Immunodeficiency V...

Ginger is one of the cash crops grown worldwide, and consumed daily as a spice food, and utilized as Ayurvedic medicine. Soft-rot or rhizome-rot, is a major rhizome-deteriorating fungal disease caused by various fungi like Fusarium spp. and Pythium spp. in ginger, leading to huge yield losses and economic losses. This study reported in vitro antifu...

Aspergillus infections are one of the significant threats to crop plants such as peanuts, cottonseed, maize, tree nuts, food, and feed. The fungal members of this genus can cause up to 100% losses in fruit plants under favorable conditions. It is thus a necessity to control these phytopathogenic fungi by a renewable, non-hazardous technology. Phoma...

Antimicrobial resistance (AMR) is a main public health issue and a challenge for the scientific community all over the globe. Hence, there is a burning need to build new bactericides that resist the AMR. The ZnONPs were produced by cell free extract of mint (Mentha piperita L.) leaves. Antibiotics that are ineffective against resistant bacteria lik...

Being an essential element for life on Earth water is required for everyday activities as well as major industrial applications, including agriculture. However, major anthropogenic activities, ranging from agriculture to the textile industry and many others, are leading to the release of numerous quantities of chemical effluents and dyes into water...

The genus Phoma has been explored for a wide range of secondary metabolites signifying a huge range of bioactivities. Phoma sensu lato is a major group that secretes several secondary metabolites. The genus Phoma mainly includes Phoma macrostoma, P. multirostrata, P. exigua, P. herbarum, P. betae, P. bellidis, P. medicaginis, P. tropica, and many m...

The microflora of the soil is adversely affected by chemical fertilizers. Excessive use of chemical fertilizers has increased crop yield dramatically at the cost of soil vigor. The pH of the soil is temporarily changed by chemical fertilizers, which kill the beneficial soil microflora and can cause absorption stress on crop plants. This leads to hi...

Citation: Pete, A.M.; Ingle, P.U.; Raut, R.W.; Shende, S.S.; Rai, M.; Minkina, T.M.; Rajput, V.D.; Kalinitchenko, V.P.; Gade, A.K. Biogenic Synthesis of Fluorescent Carbon Dots (CDs) and Their Application in Bioimaging of Agricultural Crops. Nanomaterials 2023, 13, 209. https://doi. Abstract: Fluorescent nanoparticles have a transformative potentia...

Agriculture is a backbone of global economy and most of the population relies on this sector for their livelihood. Chitosan as a biodegradable material thus can be explored for in various fields in its nano form to replace non-biodegradable and toxic compounds. The chitosan has appealing properties like biocompatibility, non-toxicity, biodegradabil...

Green synthesis is an eco-friendly approach of nanoparticles fabrication gaining momentum among researches. Nanoparticles are used immensely, due to its small size, physical properties, orientation, which can apparently change the performance of any other material when in proximity. Antibacterial, antioxidant, antifungal, antiviral, anti-inflammato...

Wound healing is a complex phenomenon particularly owing to the rise in antimicrobial resistance. This has attracted the attention of the scientific community to search for new alternative solutions. Among these, silver being antimicrobial has been used since ancient times. Considering this fact, the main goal of our study was to evaluate the wound...

The increasing multidrug-resistance in pathogenic microbes and the emergence of new microbial pathogens like coronaviruses have necessitated the discovery of new antimicrobials to treat these pathogens. The use of antibiotics began after the discovery of penicillin by Alexander Fleming from Penicillium chrysogenum. This has attracted the scientific...

Aim: The main aim of the present study was to develop nanotechnology-based solutions for the management of a fungus, Phytophthora parasitica causing gummosis in citrus. Methods and Results: Biogenic copper nanoparticles (CuONPs) were synthesized using two different biocontrol agents, Pseudomonas fluorescens and Trichoderma viride and characterized...

Aim: The main aim of the present study is to develop nanotechnology-based solutions for the management of a fungus, Phytophthora parasitica causing gummosis in citrus. Methods and results: Biogenic copper nanoparticles (CuONPs) were synthesized using two different biocontrol agents, Pseudomonas fluorescens and Trichoderma viride and characterize...

There is a great demand for green methods of synthesis of nanoparticles. Fungi play an important role in the synthesis of nanoparticles, of which Aspergillus spp. are known to secrete different enzymes responsible for the synthesis of nanoparticles. The process of biosynthesis of nanoparticles is simple, rapid, cost-effective, eco-friendly, and eas...

Fabrication of metal nanoparticles till date has been reported by the use of several biological agents like bacteria, fungi, yeast, algae, and plants. Among all the biological systems used for the synthesis of metal nanoparticles, fungal system scores over the other biological system. The possible use of fungi has gained much importance, as they ar...

Accumulation of toxic contaminants and heavy metals in the environment is among the major problems causing pollution. They cause contamination of air, soil, and water. It ultimately leads to the development of hazardous effects on the humans and other organisms. They are quite difficult to recover from the environment. Traditionally those pollutant...

The microbial infections due to biofilm forming bacterial pathogens are very common in human subjects. The intensive application of antibiotics in integrated disease management strategy has led to increased multidrug resistance incommon pathogens. Thus, indicating need of developing an alternative method for the control of these multidrug resistant...

Introduction: Alternaria spp. and Candida spp. are the main fungal pathogen of indoor environment like house, office, classroom, etc. These may cause various diseases and infections like systemic infections, or chronic asthma in immunocompromised individuals through secretion of various toxic substances. Chemical-based commercially available room f...

Nanobiotechnology opens up the new avenue in the area of nanoscience and nanotechnology. The use of different and available biological resources in the fabrication of nanoparticles is a recent trend in the scientific community. Owing to the antimicrobial properties of silver and gold nanoparticles (NPs), these are routinely used in various antimicr...

Fungal infections are major issue in agricultural crop plants that affects the growth of plant causing huge economic losses. Silver nanoparticles (AgNPs) have shown antimicrobial effects. Here, we report the potential application of phytostabilized AgNPs as a novel nanofungicide and growth promoter in Vigna radiata (L.) R. Wilczek (Mung). The aqueo...

The coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a global health problem that the WHO declared a pandemic. COVID-19 has resulted in a worldwide lockdown and threatened to topple the global economy. The mortality of COVID-19 is comparatively low compared with previous SARS outbreaks, b...

The applications of metal-based nanoparticles (MNPs) in the sustainable development of agriculture and food security have received greater attention in recent years in the science community. Different biological resources have been employed to replace harmful chemicals to reduce metal salts and stabilize MNPs, i.e., green methods for the synthesis...

Abstract A large population is suffering from multifactorial urolithiasis worldwide with a reoccurrence rate of almost 70%–80% in males and 47%–60% in females. In the present study, the nephroprotective effect of silver nanoparticles (AgNPs) synthesised by Bryophyllum pinnatum was evaluated in ethylene glycol‐induced urolithiasis in rat. B. pinnatu...

Nanotechnology is a new and developing branch that has revolutionized the world by its applications in various fields including medicine and agriculture. In nanotechnology, nanoparticles play an important role in diagnostics, drug delivery, and therapy. The synthesis of nanoparticles by fungi is a novel, cost-effective and eco-friendly approach. Am...

Phytopathogens are responsible for huge losses in the agriculture sector. Amongst them, fungal phytopathogen is quite difficult to control. Many chemicals are available in the market, claiming the high activity against them. However, the development of resistance by the fungal pathogen is the main concern to overcome their menace. Nanotechnology-ba...

Nanotechnology is a broad and multidisciplinary area of research, which is growing explosively worldwide. Nanoparticles are considered as basic or fundamental units of nanotechnology, arising the need to develop safer, reliable, clean, and eco-friendly methods for the fabrication of these nanoparticles. The fabrication of nanoparticles is an import...

Endophytes are those inhabiting in plants without causing any apparent loss to the host plant. Phoma is a ubiquitously found genus of fungi in soil, water and air. Endophytic Phoma spp. are distributed with high specific diversity, those occur in plants and are mainly responsible for the production of a vast range of secondary metabolites. These se...

Food security is a high-priority issue for sustainable global development for both quantitatively and qualitatively parameters. The increases in production and application of nanomaterials and their releases in the environment raise concern for human health through/via the food chain. A terrestrial environment, especially soil is expected to be the...

The evaluation of toxic effects of nanoparticles (NPs) has become an important aspect of Nanotechnology research in the 21st century. The present investigation deals with the green synthesis of biogenic zinc oxide nanoparticles (ZnO-NPs) using Bryophyllum pinnatum leaves, their characterization and evaluation of acute oral toxicity in Wistar rats....

Nanoscience and nanotechnology offer an exciting prospect to study a state of matter that is intermediate between bulk and isolated atoms or ions, as well as the spatial confinement effect on electron behavior. It also provides an opportunity to investigate the problems associated with surface or interface because of their interfacial nature (Rai a...

Here, a rapid and easy transformation by electroporation technique for gene transfer in plants using cell penetrating amino nanocomplex (nanoplex) has been demonstrated in Nicotiana. Nanoplex was prepared using cell penetrating amino acids (CPAs) such as poly-L-lysine (PLL) and Argenine (Arg), in combination with the gold nanoparticles (AuNPs). PLL...

In this article, we have reported surface roughness of silver nano particle treated handmade paper sheets using electronic speckle pattern interferometer (ESPI). Paper sheets are specially designed for showing antimicrobial activities. Surface roughness values of nano particle treated paper sheets were compared with untreated paper sheets. Typical...

Background Copper is an important micronutrient required for the growth of the plants. It activates enzymes and helps in protein synthesis in plants. Nanoparticles in the size range from 1 to 100 nm possess unique properties, such as high surface area to volume ratio, size dependent capabilities and unique optical properties, and hence, copper nano...

The genus Phoma contains several species ubiquitously present in soil, water, and environment. There are two major groups of Phoma, viz., terrestrial and marine. After 1981 researchers all over the world have focused on marine-derived Phoma for their bioactive compounds. The marine Phoma are very rich sources for novel bioactive secondary metabolit...

Food spoilage is a major global concern due to the lack of proper packaging technology. Nanotechnology is expected to improve food packaging. Indeed, novel nano-based food packaging materials possess unique characteristics including antimicrobial potential, oxygen scavengers, and barriers to the gas or moisture, etc. The application of such nanomat...

This book is organized into 18 chapters covering the following subjects: history (Chapter 1); use of nanomaterials in agriculture (Chapter 2); green nanotechnology (Chapter 3); nanonutrients (Chapter 4); enhanced productivity (Chapter 5); synthesis and applications of nanoparticles in agriculture (Chapter 6); toxicity, fate and transport of nanomat...

Environmental pollution and toxicity have been increasing due to the overuse of chemical fertilisers, which has encouraged nanotechnologists to develop eco-friendly nano-biofertilisers. The authors demonstrated the effect of biogenic copper nanoparticles (CuNPs) on the growth of pigeon pea (Cajanus cajan L.). The UV-visible analysis showed absorban...

Background: In recent years, the agro-bionanotechnology has paved the way towards revolutionizing the current practices of food and agricultural fields. In the present study, the effect of copper nanoparticles alone and in combination with growth promoting fungus Piriformospora indica on two-week old seedlings of Cajanus cajan was evaluated. Gelat...

There is a demand for environmentally friendly processes to synthesize nanoparticles. Here, we synthesized silver nanoparticles using encapsulated biomass beads of Phoma exigua var. exigua. Nanoparticles were characterized by nanoparticle tracking and analysis (NTA), Fourier-transform infrared spectroscopy, X-ray diffraction, transmission electron...

In the present study, the extracellular phycofabrication (synthesis by algae) of silver nanoparticles was demonstrated using algae i.e. Spirogyra sp. recovered from the fresh water. The reduction of silver ions present in the aqueous solution of silver sulphate (Ag2SO4) was done by the cell filtrate of Spirogyra sp. leading to the synthesis of silv...

Nanoparticle (NPs) containing essential metals are being considered in formulations of fertilizers to boost plant nutrition in soils with low metal bioavailability. This paper addresses whether colonization of wheat roots by the bacterium, Pseudomonas chlororaphis O6 (PcO6), protected roots from the reduced elongation caused by CuO NPs. There was a...

Association of Cu with unwashed coleoptiles after growth in sand with CuO NPs. /kg sand. (DOCX)

Effect of CuO NPs addition to growth matrix on coleoptile growth. (DOCX)

Culturability of cells of wild type PcO6 or its gacS mutant recovered from 7 d-old wheat seedlings grown in the presence of 0, 100 or 300 mg Cu from CuO NPs. (DOCX)

Background fluorescence of wheat root tips under imaging conditions used for fluorescence from DHE and Sytox Blue (SB), BODIPY (BD) and Dark Red (DB). (DOCX)

Gene specific primer pairs used for qRT-PCR. (DOCX)

Effect of PcO6 root colonization on shoot height in the presence of 300 mg Cu from CuO NPs/kg sand. (DOCX)

Contamination of outer but not inner surfaces of coleoptiles with CuO NPs. (DOCX)

Experiment on storage stability and performance of aqueous and dry formulation of Helicoverpa armigera nuclear polyhedrosis virus (HaNPV) was conducted during 2011-2013 in Bio control laboratory, Department of Entomology and Insect Biotechnology laboratory, Biotechnology Centre, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola with an objective to d...

Experiment on storage stability and performance of aqueous and dry formulation of Helicoverpa armigera nuclear polyhedrosis virus (HaNPV) was conducted during 2011-2013 in Bio control laboratory, Department of Entomology and Insect Biotechnology laboratory, Biotechnology Centre, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola with an objective to d...

The present study reports the synthesis of silver nanoparticles (AgNPs) using both biological and chemical routes to find out the best method for control of their size and activity. The fungal agent (Fusarium oxysporum) and the plant (Azadirachta indica) were found to be the best source for AgNPs synthesis. Both biosynthesis and chemosynthesis were...

Aim: The authors report the biological synthesis of zinc oxide nanoparticles (ZnO-NPs) from the petals extract of Rosa indica L. (rose). Its efficacy was evaluated against two dermatophytes: namely: Trichophyton mentagrophytes and Microsporum canis which cause onychomycosis. The activity of antibiotics against the tested dermatophytes was enhanced,...

In spite of the limited sources of fossil fuels, energy demand has been considerably increased since the last century. The problems associated with global warming due to rising atmospheric greenhouse gas levels and scarcity of fossil fuels make it imperative to reduce our heavy dependency on fossil fuels. These reasons forced countries throughout t...

Cost-effective “green” methods of producing Ag nanoparticles (NPs) are being examined because of the potential of these NPs as antimicrobials. Ag NPs were generated from Ag ions using extracellular metabolites from a soil-borne Pythium species. The NPs were variable in size, but had one dimension less than 50 nm and were biocoated; aggregation and...

Aim: We report Myxobacteria virescens mediated synthesis of silver nanoparticles (AgNPs) and its efficacy against Staphylococcus aureus (ATCC-33591), Salmonella typhi (ATCC-51812), Escherichia coli (ATCC-14948), Klebsiella pneumoniae (MTCC-4030) and Pseudomonas aeruginosa (MTCC-4673). The organism exhibiting resistance to various antibiotics showed...

The gradual increase in bacterial resistance to prevalent antimicrobial therapies is a severe global threat. In this context, there is an urgent need to search for alternative strategies to cope with drug resistance in human pathogens worldwide. Antimicrobial nanoparticles have gained considerable scientific attention as potent bioagents to restric...

Curcumin is one of the polyphenols, which has been known for its medicinal use since long time. Curcumin shows poor solubility and low absorption, and therefore, its use as nanoparticles is beneficial due to their greater solubility and absorption. The main aim of the present study was the formation of curcumin nanoparticles (Nano curcu), evaluatio...

With the rise in human population across the globe especially in developing countries, the incidence of microbial infections are increasing with greater pace. On the other hand, available medication and therapies are found to be insufficient for the complete cure of such microbial infections due to the development of resistance against various anti...

In India more than 60 % of the population relies on crops for their livelihood. However, crop diseases are one of the major factors limiting productivity. Hence, nanotechnology appears as a new means to control diseases and enhance yield. Here, stable copper nanoparticles were synthesized using cetyl trimethyl ammonium bromide and copper nitrate at...

Many scientists have focused their research on the role of nanotechnology for the control of human pathogens, but there are also many topical pathogens present in animals, which infect animals and transfer to humans. Topical therapy is extremely important for the management of dermatological condition in animals. Therefore, the present study aims t...

Present investigation was carried out to test the potency of Silver nanoparticles (SNPs) as UV protectants along with tinopal and mango leaf extract at Department of Entomology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola during 2011 - 2013. Formulated viral suspension was exposed to UV 20 W Philips lamp (Type 05, range 285 - 320 nm) for 15 min...

The authors report extracellular mycosynthesis of silver nanoparticles (AgNPs) by Phoma capsulatum, Phoma putaminum and Phoma citri. The AgNPs thus synthesised were characterised by UV-visible spectrophotometer, Fourier transform infrared spectroscopy, Nanosight LM20 and transmission electron microscopy, which confirmed the synthesis of mostly sphe...

Curcumin (diferuloylmethane) is one of the potent, nontoxic, and major bioactive components present in turmeric. The major drawbacks of curcumin are low absorption and poor bioavailability. The present review highlights on the methods for the fabrication of curcumin nanoparticles and their applications in treatment of cancer and wound infections. C...

We report an eco-friendly method for the synthesis of copper nanoparticles (CuNPs) using Citron juice (Citrus medica Linn.), which is nontoxic and cheap. The biogenic copper nanoparticles were characterized by UV-Vis spectrophotometer showing a typical resonance (SPR) at about 631 nm which is specific for CuNPs. Nanoparticles tracking analysis by N...

Baculovirus could be an attractive candidate for integrated pest management (IPM) because of its inherent high pathogenicity, narrow host range, and safety to vertebrates, plants and the environment. Despite these advantages, its practical application as a microbial pesticide has not been fully exploited due to rapid inactivation of the virus by su...

Curcumin (diferuloylmethane) is one of the potent, non-toxic and major bioactive components present in turmeric. Major drawbacks of curcumin are low absorption and poor bioavailability. The present review highlights on methods for the fabrication of curcumin nanoparticles and their applications in treatment of cancer and wound infections. Curcumin...

The production of transgenic plants is considered as a valuable tool in plant research and the technology is comprehensively useful in agricultural research. Gene transfer in plants is generally carried out by Agrobacterium sp., application of some chemicals, and physical techniques (electroporation, microprojectile, etc.). Now-a-days with better e...

We studied ten-isolates of F. equiseti isolated from different fruits and vegetables, which were identified on the basis of morphological characteristics and Random amplified polymorphic DNA (RAPD). Analysis of these isolates was made by using 25 different universal decamer primers to confirm the identity. UPGMA study differentiated of ten isolates...

The present study reports the synthesis of silver nanoparticles (AgNPs) using both biological and chemical routes to find out the best method for control of their size and activity. The fungal agent (Fusarium oxysporum) and the plant (Azadirachta indica) were found to be the best source for AgNPs synthesis. Both biosynthesis and chemosynthesis were...