Ângelo C. Salvador

• University of Aveiro

Diabetes mellitus (DM) is one of the most prevalent lifestyle diseases with an increasing impact on human health. Chronic hyperglycaemia in diabetes leads to various metabolic disorders such as protein glycation, fluid and mineral imbalance, and deterioration of tissues. Currently available pharmacological treatments of DM help control glycaemia an...

Elderberry (Sambucus nigra L.) consumption has been growing in the last years, generating a large number of stalks (~10% of the berries bunch) that are still under-valorized. This study focused on the evaluation of elderberry stalks as a source of high-value phytochemicals. In this vein, the essential mineral content and lipophilic composition were...

The main goal of this study was to chemically characterize an aqueous S. nigra flower extract and validate it as a bioactive agent. The elderflower aqueous extraction was performed at different temperatures (50, 70 and 90 °C). The extract obtained at 90 °C exhibited the highest phenolic content and antiradical activity. Therefore, this extract was...

Horticultural crops are vulnerable to several disorders, which affect their physiological and organoleptic quality. For about forty years, the control of physiological disorders (such as superficial scald) in horticultural crops, particularly in fruit, was achieved through the application of the antioxidant diphenylamine (DPA), usually combined wit...

Substantial amounts of vine wastes are produced during vineyard management, and the chemical profiling of high-value lipophilic phytochemicals is becoming crucial in order to find a complementary route towards their integrated valorisation. The prospection of bioactive phytochemicals from unripe grape, vine shoot, vine cane, stalk and leaf dichloro...

Plant secondary metabolites are synthesized for their protection and regulation purposes. Quite often, due to their properties, these metabolites have relevant organoleptic and biological properties and can play important roles in human health and general well-being. A relevant case study in this context is berries and flowers from Sambucus nigra L...

The volatile terpenic and norisoprenoids profile from elderflowers (Sambucus nigra L.) was established for two cultivars by multidimensional gas chromatography. From 47 monoterpenic, 13 sesquiterpenes and 5 norisoprenoids components, 38 are reported for the first time on elderflowers. Elderflower seasonality implies proper handling and storage cond...

Elderberry (Sambucus nigra L.) lipophilic and polar extract dietary supplementation effects were evaluated according to diabetes management indices, using an in vivo model. A research pipeline was constructed, that ranged from extract preparation, partial chemical characterization and toxicity evaluation, to examining the elderberry extract dietary...

The integration of plant metabolomics to support preharvest fruit development studies can provide important insights into the biochemical mechanisms involved and lately support producers on harvesting management. A metabolomic-based strategy for fingerprinting of volatile terpenoids and norisoprenoids from Sambucus nigra L. berries from three culti...

The chemical composition of the lipophilic extracts of three Portuguese elderberries cultivars belonging to the Sambucus nigra L. species (‘Bastardeira’, ‘Sabugueira’ and ‘Sabugueiro’), was studied by gas chromatography–mass spectrometry. The influence of the harvesting season (2012 and 2013), cultivar and the ripening stage was evaluated. Regardin...

The lipophilic composition of wild Arbutus unedo L. berries, collected from six locations in Penacova (center of Portugal), as well as some general chemical parameters, namely total soluble solids, pH, titratable acidity, total phenolic content and antioxidant activity was studied in detail to better understand its potential as a source of bioactiv...

Lipophilic constituents (triterpenoids, fatty acids, fatty alcohols, and sterols) from the berries of the three major Sambucus nigra L. cultivars grown in Portugal ('Sabugueira', 'Sabugueiro', and 'Bastardeira') were characterized by gas chromatography-mass spectrometry. Triterpenoids were the most abundant constituents, accounting for up to 92% of...

Metabolomics has expanded on the study of medicinal plants namely on the secondary metabolites plant profiling, that are linked to diverse health benefits on humans, arising as a suitable approach to evaluate biochemical-mediated responses. Elderberries have been used for generations in folk medicine, with several health benefits being reported [1]...

In the last years, new approaches use scats analysis in ecological studies, like gas chromatographic techniques, has been developed. The aim of this study is to use VOCs (volatile organic compounds) released from scats to distinguish three mammal species: the ungulates red and fallow deer and the carnivore Eurasian otter through the application of...

Elderberries and the respective juice from them (Sambucus nigra and S. canadensis) have antioxidant, anti-viral, anti-hypertensive and other health effects due to the phenolic components (including anthocyanins), terpenoids and proteins (lectins) among others. They also have a diuretic effect and can relieve constipation. Moreover, they offer prote...

The effect of high pressure on salt and water diffusion in the desalting process of cod was studied. Under pressure, up to 300 MPa, the osmotic equilibrium is reached much faster, compared to desalting at atmospheric pressure. Water (D ew) and salt (D es) effective diffusion coefficients reached a maximum at 200 MPa, increasing 500- and 160-fold, r...

A novel approach based on headspace solid-phase microextraction (HS-SPME) combined with comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry (GC×GC–ToFMS) was developed for the simultaneous screening of microbial and mite contamination level in cereals and coffee beans. The proposed approach emerges as a powerful tool f...

A microextracção em fase sólida (SPME) combinada com a cromatografia de gás (GC) apresenta um elevado potencial de aplicação, quer no estabelecimento de perfis voláteis globais de uma dada amostra, quer na quantificação de analitos alvo. Essa combinação tem sido utilizada em inúmeras áreas, nomeadamente na caracterização de produtos naturais, alime...

The aim of this study was to investigate the influence of monospecific colonization of sediment stands by Spartina maritima or Halimione portulacoides on benthic prokaryote assemblages in a salt-marsh located in Ria de Aveiro (Portugal). The distribution of Bacteria, Archaea and sulfate-reducing bacteria (SRB) in sediments with monospecific plant s...

During beer production, xanthohumol (XN) extracted from hop through wort boiling is largely converted into its isomeric flavanone, isoxanthohumol (IXN), due to the thermal treatment. XN has a great biological interest as a functional compound (being anti-carciogenic, antioxidant, anti-inflammatory, and anti-infective) and its loss leads to beers wi...

This work aims to compare the accuracy of several drying modelling techniques namely semi-empirical, diffusive and artificial neural network (ANN) models as applied to salted codfish (Gadus Morhua). To this end, sets of experimental data were collected to adjust parameters for the models. Modelling of codfish drying was performed by resorting to Pa...

The effect of the ionic liquid 1-butyl-3-methylimidazolium chloride ([bmim]Cl) and of high pressure on the activity of cellulase from Aspergillus niger were studied separately and in combination. The enzyme activity decreased with increasing concentrations of [bmim]Cl, reaching 50% the value in aqueous buffer with 20% [bmim]Cl. However, when the en...