Angeline Rouers

Angeline Rouers
Agency for Science, Technology and Research (A*STAR) | A*Star · Infectious Diseases Labs

PhD

About

33
Publications
5,877
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308
Citations
Introduction
Angeline Rouers currently works at the A*STAR Infectious Diseases Labs (Singapore). Angeline does research in Human Biology, Immunology and Cell Biology. Her current research focus is on COVID-19 and mosquito-borne diseases as dengue and malaria.

Publications

Publications (33)
Article
Full-text available
Understanding the impact of age on vaccinations is essential for the design and delivery of vaccines against SARS-CoV-2. Here, we present findings from a comprehensive analysis of multiple compartments of the memory immune response in 312 individuals vaccinated with the BNT162b2 SARS-CoV-2 mRNA vaccine. Two vaccine doses induce high antibody and T...
Preprint
Full-text available
Background On 26 November 2021, the World Health Organization designated the B.1.1.529 lineage of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) as the fifth variant of concern, Omicron. Infections have quickly spread worldwide, but understanding of the viral dynamics and the cytokine and cellular immunological response during infecti...
Article
Full-text available
The SARS-CoV-2 Delta (B.1.617.2) variant is capable of infecting vaccinated persons. An open question remains as to whether deficiencies in specific vaccine-elicited immune responses result in susceptibility to vaccine breakthrough infection. We investigated 55 vaccine breakthrough infection cases (mostly Delta) in Singapore, comparing them against...
Article
Full-text available
Objective: Despite the high vaccine efficacy of mRNA COVID-19 vaccines, there are individuals who developed excessive reactogenic and/or allergic responses after the first mRNA dose and were considered ineligible for further mRNA doses. CoronaVac, an inactivated SARS-CoV-2 vaccine, is recommended in Singapore as an alternative. Methods: Individu...
Preprint
Full-text available
Efficient COVID-19 vaccines have been developed in record time. Here, we present findings from a comprehensive and integrated analysis of multiple compartments of the memory immune response in 312 individuals vaccinated with the BNT162b2 mRNA vaccine. Two vaccine doses induced high antibody and T cell responses in most individuals. However, antibod...
Article
Full-text available
Prior immunological exposure to dengue virus can be both protective and disease-enhancing during subsequent infections with different dengue virus serotypes. We provide here a systematic, longitudinal analysis of B cell, T cell, and antibody responses in the same patients. Antibody responses as well as T and B cell activation differentiate primary...
Article
Full-text available
Clinically important broadly reactive B cells evolve during multiple infections, with B cells re-activated after secondary infection differing from B cells activated after a primary infection. Here we studied CD27highCD38high plasmablasts from patients with a primary or secondary dengue virus infection. Three transcriptionally and functionally dist...
Article
T cells play important multifaceted roles during dengue infection, and understanding their responses is important for defining correlates of protective immunity and identifying effective vaccine antigens. Using mass cytometry and a highly multiplexed peptide-HLA (human leukocyte antigen) tetramer staining strategy, we probed T cells from dengue pat...
Article
Full-text available
Regulatory T cells (Tregs) play a major role in immune homeostasis and in the prevention of autoimmune diseases. It has been shown that c-Rel is critical in Treg thymic differentiation, but little is known on the role of NF-κB on mature Treg biology. We thus generated mice with a specific knockout of RelA, a key member of NF-κB, in Tregs. These mic...
Article
Full-text available
Follicular helper T cells (Tfh) have been discovered in lymph nodes and, since then, are the focus of very intensive research to understand their origin, differentiation and functions. Tfh interact with B cells in the secondary lymphoid organs leading to B cell differentiation and maturation. Tfh are particularly studied in pathological contexts su...
Data
Proportions of memory B cell populations in HIV+ patients and HIV − donors. (a) Frequency of total memory B cells (CD27+ IgD −) among CD19+ B cells. (b) Frequency of naïve B cells (CD27 − IgD+) among CD19+ B cells. (c) Frequency of IgG + cells among memory B cells. (d) Frequency of IgG2+ cells among memory B cells. (e) Frequency of CD38hi plasmabla...
Data
Distribution and frequency of B cells according to their Ab isotype in HIV+ and HIV − donors. Frequencies of B cells secreting IgG (a), IgG1 (b), IgG2 (c) or IgG3 (d) among total B cells in EC (circle: HLA-B*57+ or square: HLA-B*57 −), cART and HIV-negative donors. Each individual is represented by a specific dot on each graph (shape and color). St...
Data
Memory B cell responses against Influenza vaccine antigens. Frequency of Flu-specific IgG+ B cells in EC, cART and HIV-negative donors. Each individual is represented by a specific dot on each graph (shape and color). Circle: HLA-B*57+ EC; square: HLA-B*57 − EC. Statistical significance was calculated using a Kruskal-Wallis test followed by a Dunn’...
Data
Proportion of HLA-B57+ and HLA-B57 − EC responding to viral antigens. HLA-B57+ EC (n = 16) are represented in green and HLA-B*57 − (n = 17) in orange. Proportions of patient presenting (a) IgG +, (b) IgG1 +, (c) IgG2 + and (d) IgG3 + B cell responses against HIV-Env antigens (gp140Yu2b, gp41S30 or gp160THO) and Influenza vaccine antigens (Flu, VAXI...
Data
Phenotypic analysis of circulating B cell populations using flow cytometry. B cells were identified using CD19, and further subdivided in naïve (CD27–IgD+), total memory B cells (CD27+ IgD–), activated memory (AM, CD27+ CD21 −), resting memory (RM, CD27+ CD21+), intermediate memory (IM, CD27− CD21+), tissue-like memory (TLM, CD27 − CD21 −), plasmab...
Data
The quantity of HIV-specific IgG in patient’s sera does not correlate with HIV-specific B cell frequency. (a) Quantity of HIV-specific IgG normalized to total IgG, evaluated using gp41 and gp140 HIV antigens, in sera from HLA-B*57 + (yellow circles) and HLA-B*57 − (pink squares) EC. Bars indicate median values. (b) Spearman correlation between the...
Article
Full-text available
A fraction of HIV-1-infected individuals (so-called elite controllers, ECs) naturally control HIV-1 replication maintaining undetectable viral loads. Understanding the mechanisms implicated in natural control of HIV-1 infection will help in developing efficient HIV vaccines. In ECs, we analyzed the influence of B cell antibody responses. We show th...
Article
Full-text available
The discovery of broad and potent HIV-1 neutralizing antibodies (bNAbs) has renewed optimism for developing an effective vaccine against HIV-1. The generation of most bNAbs requires multiple rounds of B cell receptor affinity maturation, suggesting a crucial role of follicular helper T (Tfh) cells in their production. However, less than 1% of HIV-i...
Thesis
Full-text available
La réponse humorale est altérée lors de l’infection par le virus de l’immunodéficience humaine (VIH). Les lymphocytes T CD4+ folliculaires helper (Tfh) sont impliqués dans la maturation des lymphocytes B (LB) dans les organes lymphoïdes secondaires. Mon travail de thèse s’est articulé autour de deux axes complémentaires visant à étudier les Tfh et...
Article
Full-text available
It is widely assumed that CD4(+) T cells recognize antigenic peptides (epitopes) derived solely from incoming, exogenous, viral particles or proteins. However, alternative sources of MHC class II (MHC-II)-restricted Ags have been described, in particular epitopes derived from newly synthesized proteins (so-called endogenous). In this study, we show...
Article
(75 words max) Adoptive cell therapy represents a promising approach for several chronic diseases. This study describes an innovative strategy for biofunctionalization of nanoparticles, allowing the generation of synthetic particulate antigens (SPAg). SPAg activate polyclonal B cells and vectorize non-cognate proteins into their endosomes, generati...
Article
Full-text available
Follicular helper T (Tfh) cells within secondary lymphoid organs control multiple steps of B cell maturation and antibody (Ab) production. HIV-1 infection is associated with an altered B cell differentiation and Tfh isolated from lymph nodes of HIV-infected (HIV+) individuals provide inadequate B cell help in vitro. However, the mechanisms underlyi...
Article
Full-text available
CD4(+)CD25(+)Foxp3(+) regulatory T (Treg) cells play a major role in peripheral tolerance. Multiple environmental factors and cell types affect their biology. Among them, activated effector CD4(+) T cells can boost Treg cell expansion through TNF or IL-2. In this study, we further characterized this effector T (Teff) cell-dependent Treg cell boost...

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Project (1)
Project
We have followed a cohort of dengue-infected patients and analysed B (and T) cell responses from few days after infection until 1 year after infection.