Angelina Chalima

• Doctor of Philosophy
• National Technical University of Athens

In this study, we examine the growth of four microalgae strains of Phaeocystis sp., Pleurochrysis sp., Chlorella sp. and Nannochloropsis sp. with Mediterranean origin, which have been poorly addressed in laboratory studies under autotrophic against mixotrophic and heterotrophic conditions. Our results demonstrate that in autotro-phic conditions, th...

Recovery of omega-3 fatty acids (FAs) from microalgae constitutes a valuable alternative to fish oil, and offers the ability to isolate a single ω-3 FA, instead of a mixture. In the framework of the concept of circular economy and sustainability, in the current work, an alternative carbon source was used as feed for oleaginous microalga Crypthecodi...

The market for pectinolytic enzymes is continually expanding. In addition to the food, animal feed, textile and paper industries, pectinases have recently found application in the field of biorefinery. However, the industrial production of these enzymes is associated with high costs. Thus, the search for new source of microbial enzymes with low cul...

Crypthecodinium cohnii represents a heterotrophic microalga rich in omega-3 polyunsaturated fatty acids (PUFAs) with significant health benefits, and especially docosahexaenoic acid (DHA). In the present study, freeze-dried C. cohnii biomass, cultivated in fed-batch bioreactors with a feed of acetic acid 33% (v/v), was used. In the first set of exp...

The establishment of a sustainable circular bioeconomy requires the effective material recycling from biomass and biowaste beyond composting/fertilizer or anaerobic digestion/bioenergy. Recently, volatile fatty acids attracted much attention due to their potential application as carbon source for the microbial production of high added-value product...

Dark fermentation is an anaerobic digestion process of biowaste, used to produce hydrogen as a fuel, which however releases high amounts of polluting volatile fatty acids in the environment. In order for the process to become more competitive, the acids stream can be utilized through conversion to high added-value docosahexaenoic acid by the microa...

Omega-3 fatty acids have become a commodity of high nutritional and commercial value; intensive fishing and its environmental and social cost has led researchers to seeking alternative more sustainable ways of producing them. Heterotrophic microalgae such as Crypthecodinium cohnii, a marine dinoflagellate, have the ability to utilize various substr...

Dark fermentation is an anaerobic digestion process of biowaste, used to produce hydrogen- for generation of energy- that however releases high amounts of polluting volatile fatty acids, such as acetic acid, in the environment. In order for this biohydrogen production process to become more competitive, the volatile fatty acids stream can be utiliz...

Polyphenol oxidases (PPOs) have been mostly associated with the undesirable post-harvest browning in fruits and vegetables and with implications in human melanogenesis. Nonetheless, they are considered useful biocatalysts in the food, pharmaceutical and cosmetic industries. The aim of the present work was to characterize a novel PPO and explore its...

Volatile Fatty Acids (VFA) are small organic compounds that have attracted much attention lately, due to their use as a carbon source for microorganisms involved in the production of bioactive compounds, biodegradable materials and energy. Low cost production of VFA from different types of waste streams can occur via dark fermentation, offering a p...