Angelica Menchaca

Angelica Menchaca
University of Bristol | UB · School of Biological Sciences

Master of Arts

About

10
Publications
18,510
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116
Citations
Citations since 2016
8 Research Items
96 Citations
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20162017201820192020202120220510152025

Publications

Publications (10)
Article
Full-text available
The American crocodile (Crocodylus acutus) is a widely distributed species across coastal and brackish areas of the Neotropical region of the Americas and the Greater Antilles. Available information on patterns of genetic differentiation in C. acutus shows a complex structuring influenced by interspecific interactions (mainly hybridization) and ant...
Article
Full-text available
The tequila bat (Leptonycteris yerbabuenae) has been the focus of intense research over the last 20 years. Its close relationship to economically important plants used in the production of tequila and mezcal has raised awareness of its importance as both a keystone mutualist and a mobile link between habitats. The study of its migratory habits has...
Preprint
Full-text available
Connectivity among jaguar (Panthera onca) populations will ensure natural gene flow and the long-term survival of the species throughout its range. Jaguar conservation efforts have focused primarily on connecting suitable habitat in a broad-scale. Accelerated habitat reduction, human-wildlife conflict, limited funding, and the complexity of jaguar...
Article
Full-text available
Background: Connectivity among jaguar (Panthera onca) populations will ensure natural gene flow and the long-term survival of the species throughout its range. Jaguar conservation efforts have focused primarily on connecting suitable habitat in a broad-scale. Accelerated habitat reduction, human-wildlife conflict, limited funding, and the complexi...
Preprint
Full-text available
Background Connectivity among jaguar (Panthera onca) populations will ensure natural gene flow and the long-term survival of the species throughout its range. Jaguar conservation efforts have focused primarily on connecting suitable habitat in a broad-scale. Accelerated habitat reduction, human-wildlife conflict, limited funding, and the complexity...
Preprint
The American crocodile ( Crocodylus acutus ) is a widely distributed species across coastal and brackish areas of the Neotropical region of the Americas and the Greater Antilles. Available information on patterns of genetic differentiation in C. acutus shows a complex structuring influenced by interspecific interactions (mainly hybridization) and a...
Preprint
Background: Effective connectivity between jaguar ( Panthera onca ) populations across the American continent will ensure the natural gene flow and the long-term survival of the species throughout its range. Jaguar conservation efforts have focused primarily on connecting suitable habitat in a broad-scale. However, accelerated habitat reduction, li...
Article
Full-text available
The Andes Mountains particularly the forests along the mid-elevations of their eastern and western slopes, are a hotspot of biodiversity (high numbers of species and endemics). Among mammals, rodents are a priority group for study in the Tropical Andes given their high diversity and often relatively small geographic ranges. Here, we use DNA barcodi...
Research
Full-text available
Master Thesis - Columbia University
Article
Full-text available
Bat pups produce individually distinct isolation calls to facilitate maternal recognition. Increasing evidence suggests that, in group-living bat species, adults often use similar calls to maintain contact. We investigated if isolated adults from all three species of the highly cooperative vampire bats (Phyllostomidae: Desmodontinae) would produce...

Questions

Questions (6)
Question
Dear community. Where can I get the most up to date GIS high resolution shape files of vegetation cover for Latin America? They don’t have to be free. Specifically im looking for maps of Central America
Question
I have 7 multiplexed assays with 3 primers each that have been tagged with 6-FAM, HEX and TET. According to the Applied Biosystems website these dyes can only be read if the machine is calibrated with setup DS-34 and using TAMRA as a standard ladder in an ABI3500. However, I've been suggested that these can also be read using LIZ 500 in an ABI3730. Does any of you have any experience using these dyes and what ABI machine is most recommended? 
Question
I've used genemapper before when I've had to analyze multiplexed PCRs, I know geneious has a plugin to analyze microsats, but a few years ago it was not capable of analyzing multiplexed mstats, do you know if it can do this today? 
Question
I am trying to decide how to place some samples that will be sequenced in a Hiseq2500. I want to be sure these are randomized and that I have enough replicates to interpret my results. I know publishing this data is tricky and reviewers always have concerns about replicates. So I want your opinion before I spend a lot of money on something that wont be published later.
I am trying to validate the use of blood transcriptomes as a proxy for brain transcriptomes. My genes of interest are found in the brain but I do not have access to many of these samples, hence I will use blood. I have collected samples like so:
Brain individual A, Blood Individual A (collection site 1)
Brain individual B, Blood Individual B (collection site  1)
Brain individual C, Blood Individual C (collection site 2)
Brain individual D, Blood Individual D (collection site  2)
Blood from 12 Individuals from collection site 1
Blood from 12 individuals from collection site 2
I can afford 3 lanes in the HiSeq2500. And because I want deep coverage I will not pool more than 12 samples per lane.
Thank you!
Question
I have a multiplex PCR with 6 Fwd species specific primers and one universal reverse primer. I usually run single PCRs and after this I clean them with exosap, then cycle sequence each with each primer and then do an ethanol precipitation before sequencing in an abi3730. Do I need to add M13 tails? Or how would you think it could work the best? 
Question
I ran a 0.8% agarose gel with Ethidium Bromide to detect DNA. When analysing under UV I see bands but I also have a very dark zone impeding visualization of the migration. Is this caused by a depletion of EtBr, or even an excess of it? I tried adding EtBr to the elecrophoresis tank anode. Any suggestions to improve my gels will be much appreciated

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Projects

Project (1)
Project
Understand the molecular basis of migration in bats. I am interested in learning how environmental and genetic cues shape this behavior. I work with nectar feeding bats in Mexico.