Angel Jesús Matilla

Angel Jesús Matilla
University of Santiago de Compostela | USC · Department of Plant Physiology

Biological Sciences

About

127
Publications
46,190
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
2,323
Citations
Introduction
molecular studies on: (i) key genes involved in seed afterripening (AR) and its regulation by ABA, ethylene and GAs; and (ii) role of DOG1 gene and mannanase (MAN) gene family in the AR process.
Additional affiliations
September 1997 - present
University of Santiago de Compostela
Position
  • Professor (Full)
Description
  • We are currently studying in A. thaliana, Sisymbrium officinale and Brassica rapa: (i) key genes involved in seed afterripening (AR) and its regulation by ethylene, ABA and GAs; and (ii) role of DOG1 and mannanase (MAN) gene family in the AR process.
September 1997 - present
University of Santiago de Compostela (Spain)
Position
  • Plant Physiology Professor (Full)
Description
  • Plant Molecular Biology, Phytohormones, Plant Biotechnology
September 1997 - January 2015
University of Santiago de Compostela
Position
  • Professor (Full)
Education
May 1974 - September 1983
Universities of Santiago de Compostela, Salamanca and UAM
Field of study
  • Seed Physiology

Publications

Publications (127)
Article
Full-text available
Based on prior knowledge and with the support of new methodology, solid progress in the understanding of seed life has taken place over the few last years. This update reflects recent advances in three key traits of seed life (i.e., preharvest sprouting, genomic imprinting, and stored-mRNA). The first breakthrough refers to cloning of the mitogen-a...
Article
Full-text available
To survive in the dry state, orthodox seeds acquire desiccation tolerance. As maturation progresses, the seeds gradually acquire longevity, which is the total timespan during which the dry seeds remain viable. The desiccation-tolerance mechanism(s) allow seeds to remain dry without losing their ability to germinate. This adaptive trait has played a...
Article
The programmed cell death (PCD) occurs when the targeted cells have fulfilled their task or under conditions as oxidative stress generated by ROS species. Thus, plants have to deal with the singlet oxygen 1O2 produced in chloroplasts. 1O2 is unlikely to act as a primary retrograde signal owing to its high reactivity and short half-life. In addition...
Book
Full-text available
A set of seed dormancy traits is included in this book.
Article
Full-text available
A set of seed dormancy traits is included in this Special Issue. Thus, DELAY OF GERMINATION1 (DOG1) is reviewed in depth. Binding of DOG1 to Protein Phosphatase 2C ABSCISIC ACID (PP2C ABA) Hypersensitive Germination (AHG1) and heme are independent processes, but both are essential for DOG1's function in vivo. AHG1 and DOG1 constitute a regulatory s...
Experiment Findings
Full-text available
Auxin has recently emerged as an essential player that modulates, synergistically with ABA, different cellular processes involved in seed development as well as the induction, regulation and maintenance of primary seed dormancy (PSD). This review examines and discusses the key role of auxin as a signaling molecule that coordinates seed life. Recent...
Article
Full-text available
The production of viable seeds is a key event in the life cycle of higher plants. Historically, abscisic acid (ABA) and gibberellin (GAs) were considered the main hormones that regulate seed formation. However, auxin has recently emerged as an essential player that modulates, in conjunction with ABA, different cellular processes involved in seed de...
Article
Full-text available
DELAY OF GERMINATION-1 (DOG1), is a master regulator of primary dormancy (PD) that acts in concert with ABA to delay germination. The ABA and DOG1 signaling pathways converge since DOG1 requires protein phosphatase 2C (PP2C) to control PD. DOG1 enhances ABA signaling through its binding to PP2C ABA HYPERSENSITIVE GERMINATION (AHG1/AHG3). DOG1 suppr...
Article
Full-text available
The seed is an important organ in higher plants, it is an important organ for plant survival and species dispersion. The transition between seed dormancy and germination represents a critical stage in the plant life cycle and it is an important ecological and commercial trait....
Article
Full-text available
In higher plants, the seed precursor (ovule primordia) is composed of three parts: funiculus, nucellus and chalaza, generating the latter one (II) or two (OI and II) protective maternal integuments (seed coat, SC). The appearance of a viable seed requires the coordinate growth and development of the preceding three compartments. Integuments are ess...
Chapter
During the life cycle of plants, the demise of certain cells occurs when they have fulfilled their task. This selective and tightly regulated adaptive mechanism, known as programmed cell death (PCD), contributes to life and is fundamental for the maintenance of cellular homeostasis. Proteases are one of the key performers of PCD-mediated processes...
Research Proposal
Full-text available
Dear Colleagues, The seed, a key entity in the life cycle of higher plants, allows and ensures its survival by acquiring primary dormancy, which is hormonally induced and then maintained and strictly regulated by the modulation of a suitable hormonal signaling network. The dormancy of some seeds can be relieved through a tightly regulated process...
Article
Full-text available
Main conclusion: Mannans but not endo-β-mannanases are mainly found in the mucilage layer of two Brassicaceae seeds. Nonetheless, mannanase mobilization from inner to outer seed layers cannot be ruled out. The contribution of endo-β-mannanase (MAN) genes to the germination of the wild-type Sisymbrium officinale and cultivated Brassica rapa (Brassi...
Chapter
Full-text available
Flooding and submergence are two stresses leading to anoxic conditions in the root system. Under this stress both anoxia and hypoxia are defined by O2 shortage. Flooding negatively prevents O2 influx into the root system, thus reducing oxidative phosphorylation and ATP levels. Flood tolerant higher plant species have adequate plasticity to adjust t...
Article
Full-text available
The molecular mechanisms underlying the regulation of seed dormancy and sensu stricto germination once dormancy is removed are still largely unknown. The hormone abscisic acid (ABA), through a complex crosstalk with other plant hormones, transcription factors and cellular signals belonging to the development program, directly controls the induction...
Article
DELAY OF GERMINATION 1 (AtDOG1) was the first gene identified as dormancy-associated, but its physiological role in germination is far from being understood. Here, an orthologue of AtDOG1 in Sisymbrium officinale (SoDOG1; KM009050) is being reported. Phylogenetically, the SoDOG1 gene is included into the dicotyledonous group together with DOG1 from...
Chapter
Full-text available
Hydrogen peroxide (H 2 O 2 ) is continuously produced as a by-product of oxidative plant aerobic metabolism. It plays a dual role in cells. Under normal conditions, H 2 O 2 acts as a key regulator of many biological processes because H 2 O 2 have been identifi ed as an important second messenger in signal transduction networks. On the other hand, H...
Article
We have here demonstrated for the first time that nitrate not only accelerates testa rupture of non-AR seeds but also modifies expression pattern of the cell-wall remodeling proteins (mannanases; SoMAN6 and SoMAN7) and key genes belonging to metabolism and signaling of ABA (SoNCED6, SoNCED9, SoCYP707A2 and SoABI5) and GAs (SoGA3ox, SoGA20ox, SoGA2o...
Article
Full-text available
The softening and degradation of the cell wall (CW), often mannan enriched, is involved in several processes during development of higher plants, such as meristematic growth, fruit ripening, programmed cell death, and endosperm rupture upon germination. Mannans are also the predominant hemicellulosic CW polymers in many genera of green algae. The e...
Article
Full-text available
The transition from the dormant to the non-dormant state of a viable and mature seed can take place at low hydration by exposure to air-dry storage conditions (dry afterripening; AR). The events occurring during this loss of dormancy are of considerable physiological, ecological and agricultural interest. AR may be attributable to increased sensiti...
Article
Full-text available
The endo-β-mannanase (MAN) family is represented in the Arabidopsis genome by eight members, all with canonical signal peptides and only half of them being expressed in germinating seeds. The transcripts of these genes were localized in the radicle and micropylar endosperm (ME) before radicle protrusion and this expression disappears as soon as the...
Article
Full-text available
Mannans are hemicellulosic polysaccharides in the plant primary cell wall (CW). Mature seeds, specially their endosperm cells, have CWs rich in mannan-based polymers that confer a strong mechanical resistance for the radicle protrusion upon germination. The rupture of the seed coat and endosperm are two sequential events during the germination of A...
Article
Full-text available
The rupture of the seed coat and that of the endosperm were found to be two sequential events in the germination of Sisymbrium officinale L. seeds, and radicle protrusion did not occur exactly in the micropylar area but in the neighboring zone. The germination patterns were similar both in the presence of gibberellins (GA(4+7)) and in presence of e...
Article
Full-text available
The seed is an important organ of higher plants regarding plant survival and species dispersion. The transition between seed dormancy and germination represents a critical stage in the plant life cycle and it is an important ecological and commercial trait. A dynamic balance of synthesis and catabolism of two antagonistic hormones, abscisic acid (A...
Article
Full-text available
After-ripening (AR) in Sisymbrium officinale seeds altered SoACS7, SoACO2, SoGA20ox2, SoGA3ox2, and SoGA2ox6 gene expression. Except for SoGA20ox2 expression, which sharply diminished, the expression of the other genes rose during development, particularly that of SoACS7. In contrast, only the SoACO2 and SoGA2ox6 transcripts increased with seed des...
Article
The seed, the organ by which higher plants perpetuate themselves, is programmed to survive after being dispersed from the mother plant until establishing a photosynthetically competent seedling. Seed dormancy, conceived as the temporary failure of an intact viable seed to complete germination under favourable conditions, is developed during the las...
Article
The effects of abscisic acid (ABA) on the free polyamine: spermine (Spm), spermidine (Spd), putrescine (Put) and cadaverine (Cad) content, mitotic index (MI) and DNA synthesis have been studied in embryonic axes isolated from chick-pea (Cicer ariennum L.) seeds. Spermine and spermidine decreased in controls during the first 24 h of germination, the...
Article
The contribution of the seed and pericarp to the content of malic, quinic, citric and fumaric acids, and sucrose, fructose and glucose was determined during development and ripening of damson plum fruits. In whole fruit, (i) malic and quinic acids were the principal organic acids (OA) and their levels varied significantly, the highest being found a...
Article
Full-text available
Seed dormancy is a primary component of the different life-history strategies of annual plants. The evolution of the seed habit has permitted higher plants to colonize a large number of ecologicla niches due to the excellence of ortodox seeds as dispersal units relatively impervious to harsh environmental conditions. Seed dormancy, defined as the i...
Chapter
Full-text available
In dehiscent fruits, such as the silique of Arabidopsis thaliana, housed ripe seeds are dispersed into the surrounding environment through a process known as pod shattering. This seed-expelling process is a consequence of the partial and gradual dismantling of the silique architecture. The shattering occurs at a precise site in the silique (i.e. va...
Article
In this work, a complete cDNA encoding a polygalacturonase (Pd-PG1) has been identified by screening a cDNA library constructed from the ripe mesocarp of Prunus domestica L. subsp. insititia (damson plum). According to similarity in the amino-acid sequence and the absence of pro-sequence, Pd-PG1 belongs to a group of PGs that are expressed in fruit...
Article
In this work, four cDNA clones (Pd-ACS1,AJ890088; Pd-ETR1 and Pd-ERS1, AJ890092, AJ890091; and Pd-CTR1, AJ890089) encoding an ACC-synthase, two putative ethylene (ET) receptors, and a putative MAPKKK, respectively, were isolated and phylogenetically characterized in Prunus domestica L. subsp. insititia. Their expression was studied by real-time PCR...
Article
Full-text available
The seeds of Sisymbrium officinale are largely heterogeneous in mass and colour within the ripe fruit. In order to initiate the charac-terization of the germinative process from these heterogeneous seeds, we have used two different seed lots (i.e. brown and light-brown), and studied several physical and physiological properties and their possible i...
Article
Full-text available
Contents of total free [PA(S)] and conjugated polyamines [PA(SH), PA(PH)] were higher in turnip (Brassica rapa L. cv. Rapa) seeds during imbibition (0–36 h) and radicle protrusion (36–48 h) than during the further growth (10 d). Ethylene production was activated with the protrusion, reaching a maximum at the second day of germination and dropping a...
Article
Full-text available
A cDNA-encoding a 1-aminocyclopropane-1-carboxylic acid synthase (Ca-ACS1) was isolated and characterized from zygotic embryos of chick-pea. This gene, which belongs to a multigenic family, exhibited homology with other 1-aminocyclopropane-1-carboxylic acid synthases from several higher-plant species (e.g. Pisum sativum Ps-ACS2 and Glycine max Gm-A...
Article
Full-text available
Ethylene (ET) is a notable signaling molecule in higher plants. In the year 1993 the ET receptor gene, ETR1, was identified; this ETR1 receptor protein being the first plant hormone receptor to be isolated. It is striking that there are six ET receptors in tomato instead of five in Arabidopsis, the two best-known signaling-model systems. Even thoug...
Article
In this paper, ethylene production, ACC quantification, and the determination of ACC-oxidase (ACO) activity and the transcript levels PdACS1, PdACO1, and PdACO2, have been evaluated in different parts of developing flowers (sepals, petals, stamens, and pistils) and fruits (epicarp, mesocarp, and seed) of damson plum (Prunus domestica L. subsp. Syri...
Article
Treatment with cyclohexylamine, an inhibitor of spermidine synthase, accelerated radicle emergence in chick-pea (Cicer arietinum L. cv. Castellana) seeds. Stimulation in the growth of embryonic axis was correlated with: (a) a rise in putrescine (Put) and cadaverine (Cad); (b) a decrease in spermidine (Spd) and spermine (Spm), and (c) a concomitant...
Article
Ethylene production by isolated chick-pea embryonic axes during the initial stages of germination has been studied. Maximum production of ethylene occurs when growth is entirely due to cell elongation and before mitotic activity begins. This peak increases three-fold in the presence of calcium, but it is diminished by osmotic inhibitors, polyamines...
Article
An acid phosphatase (EC 3.1.3.2.) from the embryonic axes of chickpea seeds (Cicer arietinum L. cv. Castellana) was purified by ammonium sulphate precipitation, chromatography on Sephacryl S-200 and polyacrylamide gel electrophoresis. The preparation has an apparent molecular weight of 39 kDa, pH optimum for p-nitrophenylphosphate hydrolysis of 5.2...
Article
Nuclei and mRNA obtained from the embryonic axes of chick-pea (Cicer arietinum) seeds grown in the presence of spermine (Spm) have been used to study certain characteristics of gene expression induced by this polyamine. In the embryonic axes, free Spm varied between 100 and 300 μg (g fresh weight)−1. whereas Spm bound to macromolecules varied betwe...
Article
The flower opening of damson plum (Prunus insititia L.) was accompanied by an increase in the content of free-polyamines (PA) in the sepals, petals and sex organs, the ovary being most active in accumulating spermine (Spm). The fertilization process and senescence brought on a decline in ovarian Spm, but stimulated putrescine (Put) and spermidine (...
Article
Full-text available
Higher plants have several strategies to perpetuate themselves under adequate ecophysiological conditions. The production of heterogeneous seeds is one such strategy. That is, to ensure the survival of the next generation, an individual plant might produce seeds that are heterogeneous with respect to the extent of dormancy, dispersion and persisten...
Article
In a previous report from the present authors, it was shown that the 1-aminocyclopropane-1-carboxylate (ACC) oxidation may play a crucial role during zygotic embryogenesis of turnip tops seeds. The present study was performed to elucidate the contribution of the silique-wall and seeds in ethylene production during this developmental process. ACC co...
Article
Senescence in higher plants involves a series of physiological processes integrated within developmental programme which nomally lead to the loss of the maternal plant or most ot its organs. After senescence the plant is prepared to start the next generation. One of the facts related to senescence is the softening and failure of cell cohesion in so...
Article
During zygotic embryogenesis of turnip-tops (Brassica rapa L. cv. Rapa), the polygalacturonase activity (PG; EC 3.2.1.15), measured as a decrease in viscosity of polygalacturonic acid, reached a high when the desiccation process in the seeded silique was triggered and the valves had lost more than 70-75% of their moisture (45-50 DPA). The PG activi...
Article
Full-text available
The mature silique of turnip-tops (Brassica rapa L. cv. Rapa) contains seeds that are heterogeneous in colour. From these seeds, we have selected three homogeneous lots: black (B), dark brown (DB) and light brown (LB). The dry seeds of these lots contained different levels of free and conjugated 1-aminocyclopropane-1-carboxylic acid (ACC), polyamin...
Article
Prior to the embryogenic study of Brassica rapa (turnip tops), the PA (polyamine) changes during flower development were determined. Whereas the flower was closed, no notable changes were found. However, pollination strongly stimulated the synthesis of the F (free) and ASC (acid soluble conjugated) forms, the pistil and not the petals being the mai...
Article
The involvement of ethylene in zygotic embryogenesis is a litle known aspect of the growth and development in higher plants. The scant data available do date do not appear to indicate that ethylene is involved directly in formation of seeds. Perhaps a relationship between ethylene and abscisic acid (ABA) exist to control this phase of such importan...
Article
Full-text available
Diamine oxidase (DAO, EC 1.4.3.6.), which participates in oxidative catabolism of polyamines (PAs), was not detected in the dry viable chickpea (Cicer arietinum L.) seeds. From the time when the embryonic axis acquired an aerobic metabolism, DAO increased concomitantly with the growth of the embryonic axis and at the same time with the deterioratio...
Article
Full-text available
A recombinant protein (approximately 38 kDa by SDS/PAGE), induced by expression in Escherichia coli of a cDNA encoding a 1-aminocyclopropane-1-carboxylate oxidase (ACO) isolated from embryonic axes of Cicer arietinum, was recognized by an antibody raised against an apple ACO. A monoclonal antibody, obtained from recombinant ACO of chick-peas, was u...
Article
Full-text available
The involvement of ethylene in zygotic embryogenesis is a little known aspect of the growth and development in higher plants. In the present work, we study the alterations of the last step of the ethylene biosynthesis pathway during the formation period of turnip tops (Brassica rapa cv. Rapa) seeds and its repercussions in the germination process a...
Article
Full-text available
Abstract In seed formation the role of ethylene has received little attention. The data available on zygotic embryogenesis suggest an association of the ethylene biosynthetic pathway and seed maturation. Over the course of dicot embryogenesis, ACC-oxidase mRNA can be expressed in the cotyledons and embryonic axis. However, as maturation proceeds, c...
Article
Full-text available
Some physiological characteristics of ACC-Nmalonyltransferase (ACC-N-MTase) have been studied in the seeds of chick-pea (Cicer arietinum L.). This enzymatic activity was detectable during all periods of zygotic embryogenesis; however, the highest values were found in the dry seed. In dry seeds, the enzymatic activity was greater in the embryonic ax...
Article
Both in vivo and in vitro ACC-oxidase activities as well as ethylene production from embryonic axes of chickpea seeds were strongly inhibited by EGTA, a selective extracellular Ca2+ ion chelator, indicating that the influx of Ca2+ is important for enzymatic activity. EGTA inhibition was restored by exogenous Ca2+. Treatments of embryonic axes with...
Article
Variations in the endogenous content of polyamines, both free and conjugated to compounds of high and low molecular weight during zygotic embryogenesis in seeds of the legume Cicer arietinum were investigated for the first time. The possible role of putrescine, spermidine and spermine throughout the process of seed formation was examinated on the b...
Article
Full-text available
A full length cDNA (caaco1) encoding a putative ACC-oxidase (ACCO) was isolated and sequenced from embryonic axes of chick-pea (Cicer arietinum L.) seeds, which depend on ethylene synthesis for germination. The deduced protein for caaco1 has a molecular weight of 36 kDa, a high homology with other ACCOs and is apparently found in the cytosolic frac...
Article
Short-chain saturated fatty acids (SCSFA), ranging from pentanoic (C5) to decanoic acid (C10), when added at micromolar concentrations to the germination medium of chick-pea seeds, did not significantly affect radicle emergence, although fresh weight diminished. Only heptanoic acid (C7) and octanoic acid (C8) stimulated ethylene production and free...
Article
The activity of ACC-oxidase (ACCO) and the production of ethylene were evaluated in embryonic axes of chick-pea (Cicer arietinum) seeds incubated during 24 h in the presence of short-chain saturated fatty acids (SCSFA, C5–C10), some of which are known inhibitors of germination. Ethylene synthesis was stimulated by SCSFA C7 and C8 (10μmol/L and 100μ...
Article
Full-text available
In the present work, certain biochemical characteristics of the enzyme 1-aminocyclopropane-1-carboxylate N-malonyltransferase (ACC N-MTase) which is responsible for the malonylation of 1-aminocyclopropane-1-carboxylate (ACC) in chickpea (Cicer arietinum) are described. Phosphate buffer was the most appropriate buffer with regard to enzyme stability...
Article
This review examines the presence of free and bound PAs during the life cycle of seeds. Although data are still rather scarce, PAs, present, but with an unknown function during embryogenesis, are accumulated in relatively high quantities in mature dry seeds of some species. PA concentration can be altered by the process of stratification, and PAs a...
Article
Germination of chickpea (Cicer arietinum L.) was inhibited by supraoptimal temperatures of 30 or 35ºC, but the inhibition was alleviated by a relatively low concentration (1 mM) of putrescine (Put). This allevation may be due to (a) stimulation of the 1-aminocyclopropane-1-carboxylate (ACC) synthase and ACC oxidase activities; (b) increased levels...
Article
Polyamines (putrescine, spermidine and spermine) are aliphatic nitrogenous compounds that at present are considered growth regulators because they have some demonstrated effect on cellular growth, division and differentiation at low concentrations. Polyamines, due to polycationic nature, can be bound to negatively charged molecules such as nucleic...