Aneta A Ptaszyńska

Maria Curie-Sklodowska University in Lublin · Institute of Biology and Biochemistry

Lysozyme-type antibacterial and antifungal activity in pupae of Cameraria ohridella was studied. Activity against Micrococcus luteus and Bacillus megaterium was detected in pupae extract. Also antifungal activity from C. ohridella pupae extract directed against Saccharomyces cerevisiae strain W 303 was shown. During immunoblotting two bands in pupa...

Despite the fact that there are many studies related to the adaptogenic and pro-healthy activities of plant-based compounds, there are some adaptogenic plants whose activities are not fully known, especially those coming from the wild regions of Asia, Africa, and South America. The aim of these studies was to examine the contents of non-nutritional...

Background: Microsporidia from the Nosema (Vairimorpha) genus are pathogenic fungi that complete their life cycle in the honeybee intestine. Therefore, the aim of this study was to determine the impact of the course of infection on the viability of honeybee intestine cells. Methods and Results: Intestines isolated from healthy and N. ceranae-infect...

Due to a widespread occurrence of multidrug-resistant pathogenic strains of bacteria, there is an urgent need to look for antimicrobial substances, and honey with its antimicrobial properties is a very promising substance. In this study, we examined for the first time antimicrobial properties of novel varietal honeys, i.e., plum, rapeseed, Lime, Ph...

The aim of this study was to detect and identify the volatile compounds in coffee that was obtained in defect roast processes versus standard roasting and to determine the type and strength of the correlations between the roast defects and the volatile compound profile in roasted coffee beans. In order to achieve this goal, the process of coffee be...

Honeybees are important pollinators, but they are continuously exposed to a variety of fungal and bacterial diseases. One of the various diseases affecting honeybees is nosemosis caused by microsporidia from the Nosema genus. Honeybees are mainly infected through consumption of infected food or faeces containing Nosema spp. spores. Nosemosis causes...

Honeybees are very important pollinators, and their role in ecosystems cannot be overestimated. Apis mellifera workers pollinate approximately 80 percent of flowering plants; only in the United States are efforts estimated up to $15 billion worth of crops annually performed. Moreover, honeybees produce honey, wax, pollen, royal jelly, and propolis,...

Eleutherococcus senticosus (Rupr. et Maxim.) Maxim. is well-known for its adaptogenic properties in traditional Eastern medicine. It has been categorized as an endangered species due to the over-exploitation of the roots. As a result, alternatives must be found, including the usage of renewable aerial parts such as fruits. The goal of this research...

Nowadays, there is an urgent need to look for antimicrobial substances due to a widespread occurrence of multidrug resistant bacterial and fungal strains. Honey with its antimicrobial properties is a very promising substance with many valuable properties. In this study we examined for the first time antimicrobial properties of novel varietal honeys...

Living in an epoch of forcible human-instigated transformations, we can witness their effects both on the Earth's geology and its ecosystems. So significant are these changes that we are often said to be witnessing the birth of a new epoch-the Anthropocene. This era is believed to be driven by the "Great Acceleration" that sees human population gro...

Fall armyworm, Spodoptera frugiperda, entered Thailand in late 2018 and has now spread in several regions, with devastating effects in maize and rice production, which are some of the most important cereals in the world. Since then, farmers have utilized the available chemical insecticides to try to control it, but their efforts have been futile. I...

Honeybee symbionts, predominantly bacteria, play important roles in honeybee health, nutrition, and pathogen protection, thereby supporting colony health. On the other hand, fungi are often considered indicators of poor bee health, and honeybee microbiome studies generally exclude fungi and yeasts. We hypothesized that yeasts may be an important as...

Dermatophytes are a group of eukaryotic microorganisms characterized by high capacity to colonize keratinized structures such as the skin, hair, and nails. Over the past years, the incidence of infections caused by zoophilic species, e.g., Trichophyton verrucosum, has been increasing in some parts of the world, especially in Europe. Moreover, the e...

Buckwheat honey is widely consumed by consumers due to its numerous health-promoting properties. Characteristically it is dark, tea-like in colour, sharp, tickly, and sweet in flavour, and has smelled of buckwheat flowers. In the current study, various commercial honey samples were examined to test the quality of buckwheat honey samples available i...

This paper presents the analyses of the effect of fiber additives on volatile organic compounds in bread. The bread was baked from wheat flour with the addition of 3% of fruit fiber, following common procedures. After baking, volatile organic compounds contained in the control bread and breads supplemented with cranberry, apple, and chokeberry fibe...

Forager Apis melliefera honeybees were collected from four localities located in Europe, i.e.: London, UK; Athens, Greece; Marchamalo, Spain and Lublin, Poland. Furthermore, from Asia we have collected A. mellifera as well as A. cerana foragers form Chiang Mai in Thailand We used next generation sequencing (NGS) to analyse the 16S rRNA bacterial ge...

Eleutherococcus senticosus (Rupr. et Maxim.) Maxim. is a medicinal plant used in Traditional Chinese Medicine (TCM) for thousands of years. However, due to the overexploitation, this species is considered to be endangered and is included in the Red List, e.g., in the Republic of Korea. Therefore, a new source of this important plant in Europe is ne...

European Apis mellifera and Asian Apis cerana honeybees are essential crop pollinators. Microbiome studies can provide complex information on health and fitness of these insects in relation to environmental changes, and plant availability. Amplicon sequencing of variable regions of the 16S rRNA from bacteria and the internally transcribed spacer (I...

Taking into account that fructophilic lactic acid bacteria (FLAB) can play an important role in the health of honey bees and can be used as probiotics, phenotypic properties of probiotic interest of Lactobacillus kunkeei (12 strains) and Fructobacillus fructossus bacteria (2 strains), isolated from Apis mellifera gastrointestinal tract, have been s...

European Apis mellifera and Asian Apis cerana honeybees, are essential crop pollinators. Microbiome studies can provide complex information on health and fitness of these insects in relation to environmental changes, and plant availability. Amplicon sequencing of variable regions of 16S rRNA and internally transcribed spacers (ITSs) allow identific...

Recent years have witnessed a fall in the numbers of bee colonies worldwide. To a large extent, this is due to diseases affecting honeybees. One such disease is nosemosis, which is caused by fungi from the genus Nosema. The antibiotic fumagallin used to be administered to treat nosemosis, but as it was found capable of being transferred to bee prod...

Pollinators, the cornerstones of our terrestrial ecosystem, have been at the very core of our anxiety. This is because we can nowadays observe a dangerous decline in the number of insects. With the numbers of pollinators dramatically declining worldwide, the scientific community has been growing more and more concerned about the future of insects a...

The aim of the study was to assess the level of genetic diversity within the whole island population of Senecio macrophyllus M. BIEB. (Asteraceae) and investigate genetic distance among its particular fragments. In July 2007 leaf samples from 33 plants were collected for genetic analyses from the Biaáa Góra population (SE Poland). We used ITS regio...

Żyjemy w epoce wielkich przemian spowodowanych przez człowieka zarówno w geologii Ziemi, jak i w ekosystemach. Zmiany te są na tyle istotne, że jesteśmy świadkami narodzin nowej epoki, antropocenu. Epoka ta napędzana jest „Wielkim Przyspieszeniem”, w którym wzrostowi ulega przede wszystkim populacja ludzka, co skutkuje w drastycznym zwiększeniu zap...

Lactate is produced by active skeletal muscles. H+ is produced during lactate neutralization in the Cori cycle, which leads to muscle acidosis and sores (so called Delayed Onset Muscle Soreness – DOMS) in vertebrates. The aim of the study was to determine the activities/concentrations of compounds which may be involved in the Cori cycle in bee work...

Secondary metabolites of the roots of Eleutherococcus spp. cultivated in Poland, or the bioactivity, are not fully known. The 75% methanol extracts of five Eleutherococcus spp. ( E. senticosus , E. divaricatus , E. sessiliflorus , E. gracilistylus , and E. henryi ) were examined for the content of polyphenols and phenolic acids as well as for antia...

This paper describes taxonomic position, phylogeny, and phenotypic properties of 14 lactic acid bacteria (LAB) originating from an Apis mellifera guts. Based on the 16S rDNA and recA gene sequence analyses, 12 lactic acid bacteria were assigned to Lactobacillus kunkeei and two others were classified as Fructobacillus fructosus. Biochemically, all i...

Proper bioelement content is crucial for the health and wellness of all organisms, including honeybees. However, the situation is more complicated in these important pollinators due to the fact that they change their physiology during winter in order to survive the relatively harsh climatic conditions. Additionally, honeybees are susceptible to man...

The bioelement content [ng/mg] in honey and soil samples. Statistica (version 12.0, StatSoft Inc., USA), at the significance level of α = 0.05. NI–N. ceranae-infected honeybees. NF–N. ceranae-free honeybees. Typed in bold–data differ significantly. Table a in S1 Table. The results of comparison of bioelement content for the spring honey, the winter...

Results of the two-way ANOVA, factors: Nosema infection status and worker bee type. Statistica (version 12.0, StatSoft Inc., USA), at the significance level of α = 0.05. Factors: Nosema infection status and bee type. NHS–Nosema health status (Nosema-infected vs. Nosema-free). WBT–worker bee type (summer vs. winter). WBT*NHS–interaction of the Nosem...

Principal component analysis (PCA) of twenty two bioelements for the summer honey from different conditions. SH-NF summer honey made by Nosema-infected bees, SH-NI summer honey made by Nosema-free bees, WF-NF winter food stored by Nosema-infected bees, WF-NI winter food stored by Nosema-free bees. (a) A variable graph showing the position of the lo...

Principal component analysis (PCA) of twenty two bioelements for the spring honey from different conditions. SpH-NI spring honey made by Nosema-infected bees, SpH-NF spring honey made by Nosema-free bees, WF-NF winter food stored by Nosema-infected bees, WF-NI winter food stored by Nosema-free bees. (a) A variable graph showing the position of the...

The study of organic/inorganic molecules with activity against intracellular fungi of the phylum Microsporidia is of critical importance. Here, for the first time, the inactivation of these parasitic fungi by porphyrins is reported. The biological effects of porphyrins (10 µM and 100 µM) on the microsporidian Nosema ceranae was investigated in hone...

Bio-analysis of insects is increasingly dependent on highly sensitive methods that require high quality biological material, such as hemolymph. However, it is difficult to collect fresh and uncontaminated hemolymph from adult bees since they are very active and have the potential to sting, and because hemolymph is rapidly melanized. Here we aimed t...

Background Nosema ceranae infection not only damages honey bee (Apis melifera) intestines, but we believe it may also affect intestinal yeast development and its seasonal pattern. In order to check our hypothesis, infection intensity versus intestinal yeast colony forming units (CFU) both in field and cage experiments were studied. Methods/Finding...

The study was conducted to investigate the effect of Lactobacillus rhamnosus (a commercial probiotic) and inulin (a prebiotic) on the survival rates of honeybees infected and uninfected with Nosema ceranae, the level of phenoloxidase (PO) activity, the course of nosemosis, and the effect on the prevention of nosemosis development in bees. The cells...

The natural intestine microbiota of honeybees is dominated by Gram-negative bacteria from such families as: Enterobacteriaceae, Alcaligenaceae and Pseudomonadaceae, less numerous are Gram-positive bacteria, yeasts and other fungi. Our research was focused on the impact of commercial vertebrae probiotics, on honeybees’ intestine yeast number, hemoly...

Dietary fibres are regarded as the source of polysaccharides and antioxidants such as polyphenols. However, addition of dietary fibre to bread causes significant reduction in its quality. The bread quality is connected with the structure of gluten proteins. For this reason, Fourier transform Raman spectroscopy was applied to determine changes in st...

Spores of the microsporidian gut pathogens of honey bees Nosema apis and Nosema ceranae, the causative agents of nosemosis, are difficult to distinguish using the light microscope. This study aimed to find methods which could help to identify N. apis and N. ceranae spores, and to define morphometric parameters in order to distinguish the difference...

Nosemosis is a contagious disease of honeybees (Apis mellifera) manifested by increased winter mortality, poor spring build-up and even the total extinction of infected bee colonies. In this paper, loop-mediated isothermal amplifications (LAMP) were used for the first time to identify and differentiate N. apis and N. ceranae, the causative agents o...

In this study, the nitrogen fixing Astragalus glycyphyllos symbionts were characterized by phenotypic properties, restriction fragment length polymorphism (RFLP), and sequences of 16S rDNA. The generation time of A. glycyphyllos rhizobia in yeast extract mannitol medium was in the range 4-6 h. The studied isolates exhibited a low resistance to anti...

Streszczenie: Organizmy należące do typu mikrosporydia (Regnum: Fungi) są przyczyną wielu poważnych chorób ludzi i zwierząt. Większość gatunków mikrosporydiów, wywołujących zakaźne choroby u ssaków, może być również przyczyną infekcji u ludzi. Dlatego do badań tych groźnych patogenów został wybrany jako organizm modelowy owad – pszczoła miodna (Api...

Nosema spp. spores are extremely resistant to external stress factors and can survive several years without losing the ability for further infection within the insect body. For this reason, combating nosemosis is difficult. Some beekeepers add ethanol to the sucrose solution before the winter to prevent nosemosis infection and to cure already infec...

Nosemosis, is caused by two microsporidian species: Nosema apis and N. ceranae. In the last decade there has been rapid development and spread of bee diseases, creating serious problems for bee keeping management. The above situation requires a substantial increase in the intensity of research, which would result in a successful means of combating...

Intestinal microflora is a very important part of the digestive system in every animal, and plays a role in the synthesis of vitamins and the metabolism of many toxic chemical compounds. The indigenous intestinal flora of bees changes even as a result of changing their diet from natural to artificial or placing them in cages. Such factors have an i...

Nosemosis is a panzootic bee disease caused by two species of the genus Nosema (Fungi: Microsporidia). This disease was described for the first time in hon-eybees (Apis mellifera) 100 years ago (34). Initially, it was believed that nosemosis was caused solely by one species: Nosema apis. At the end of the twentieth century a new species of this mic...

Nosemosis, is caused by two microsporidian species: Nosema apis and N. ceranae. In the last decade there has been rapid development and spread of bee diseases, creating serious problems for bee keeping management. The above situation requires a substantial increase in the intensity of research, which would result in a successful means of combating...

Intestinal microflora is a very important part of the digestive system in all animals. It profoundly affects the anatomical, physiological and immunological de-velopment of the host, and through bacterial and fungal antagonism, inhibits intestinal colonization by exoge-nous pathogens. Furthermore, intestinal microflora benefits to the host is the s...

Nosemosis is a serious honeybee disease linked to Colony Collapse Disorder (CCD). It cause many changes at the individual bee level, which also affects the health of the entire bee colony. N. ceranae and N. apis are not tissue specific as was previously thought and besides the ventriculus epithelium their spore are also present in other tissue, suc...

Nosemosis is serious and widespread bee disease connected with Collony Colapse Disorder (CCD). Monitoring of this disease is crucial to a better understanding of the effect of this disease on the health in both individual bees and at the whole colony level. There is little information about nosemosis in different parts of Poland, which have distinc...

The systematics of the family Apionidae, as well as the superfamily Curculionoidea, is currently in a state of flux. The comparative analyses of COI sequences from our studies shed some light on the systematics of these weevils. To study the relationship among the organisms of the family Apionidae, we determined the COI sequences of representatives...