Andrey Vinokurov

Orlov State University · Department of industrial chemistry and biotechnology

Background Atherosclerosis is a complex cardiovascular disease often associated with mitochondrial dysfunction, which can lead to various cellular and metabolic abnormalities. Within the mitochondrial genome, specific mutations have been implicated in contributing to mitochondrial dysfunction. Atherosclerosis-associated m.15059G>A mutation has been...

The paper presents the results of the study of the effect of singlet oxygen induced by 1267 nm laser irradiation without the use of photosensitizers on the bioenergetics of rat RINm5F insulinoma cells.

A characteristic feature of diabetes mellitus is an increase in blood glucose levels and the development of hyperglycemia, which provokes the development of metabolic changes at the level of cells and tissues of the body. The dysfunction of pancreatic beta cells with impaired insulin secretion and a decrease in their mass is the main sign of the de...

Background Mitochondria are the main sites of cellular aerobic energy production through conjugation of respiration and oxidative phosphorylation. We have recently discovered mutations (genome variants) of mitochondrial DNA (mtDNA) associated with atherosclerosis. We have then investigated the possible mechanisms underlying such association and the...

Alteration of mitochondrial metabolism by various mutations or toxins leads to various neurological conditions. Age-related changes in energy metabolism could also play the role of a trigger for neurodegenerative disorders. Nonetheless, it is not clear if restoration of ATP production or supplementation of brain cells with substrates for energy pro...

Objective The aim of this study was to evaluate the effect of the m.15059G>A mitochondrial nonsense mutation on cellular functions related to atherosclerosis, such as lipidosis, pro-inflammatory response, and mitophagy. Heteroplasmic mutations have been proposed as a potential cause of mitochondrial dysfunction, potentially disrupting the innate im...

Neurodegenerative diseases are chronic conditions occurring when neurons die in specific brain regions that lead to loss of movement or cognitive functions. Despite the progress in understanding the mechanisms of this pathology, currently no cure exists to treat these types of diseases: for some of them the only help is alleviating the associated s...

Heat shock protein 70 (HSP70) is activated under stress response. Its involvement in cell protection, including energy metabolism and quality control makes it a promising pharmacological target. A strategy to increase HSP70 levels inside the cells is the application of recombinant HSP70. However, cell permeability and functionality of these exogeno...

Background and Aims The role of mitophagy in atherosclerosis has been extensively studied during the last few years. It was shown that mitophagy is involved in the regulation of macrophages, which are important players as immune cells in atherosclerosis development. In this study, we investigated the relationship between mitophagy and response to i...

The transmembrane receptor for advanced glycation end products (RAGE) is a signaling receptor for many damage‐ and pathogen‐associated molecules. Activation of RAGE is associated with inflammation and an increase in reactive oxygen species (ROS) production. Although several sources of ROS have been previously suggested, how RAGE induces ROS product...

Flavin adenine dinucleotide (FAD) autofluorescence from cells reports on the enzymatic activity which involves FAD as a cofactor. Most of the cellular FAD fluorescence comes from complex II of the electron transport chain in mitochondria and can be assessed with inhibitor analysis. The intensity of FAD autofluorescence is not homogeneous and vary b...

Mutations of mitochondrial DNA can have various effects on cell bioenergetics, in particular on the mitochondrial membrane potential (ΔΨm), decrease or increase of which negatively affects cell viability and can cause pathology development. A decrease in ΔΨm leads to matrix condensation and cytochrome c entry into the intermembrane space, triggerin...

Механизм влияния мутаций мтДНК на изменение окислительно-восстановительного баланса зависит от их нахождения в геноме. В то время, как повреждение гена цитохрома b вызывает снижение скорости утечки электронов в матрикс митохондрий и межмембранное пространство и соразмерное уменьшение содержания восстановленного глутатиона, мутации генов рРНК и тРНК...

This study contains analysis of existing approaches for detecting photosensitizer-free laser-induced singlet oxygen, including their ability to quantitatively measure the relationship between a dose of laser radiation and the amount of singlet oxygen produced.

This work considers the measurement of FAD fluorescence intensity as a method for the safety, simple and real-time detection of pathological cells and informative value of this approach. FAD participates in essential processes such as fatty acid oxidation, the Krebs cycle and other redox reactions. According to literature, cells in different physio...

This work investigates the influence of laser irradiation parameters (wavelength, power density and exposure time) on singlet oxygen (1O2) generation efficiency. Chemical trap (L-histidine) and fluorescent probe (Singlet Oxygen Sensor Green, SOSG) detection methods were used. Studies have been conducted for 1267, 1244, 1122 and 1064 nm laser wavele...

Alterations in function of hypoxanthine guanine phosphoribosyl transferase (HPRT), one of the major enzymes involved in purine nucleotide exchange, lead to overproduction of uric acid and produce various symptoms of Lesch-Nyhan syndrome (LNS). One of the hallmarks of LNS is maximal expression of HPRT in the central nervous system with the highest a...

Mitochondrial diseases are a large class of human hereditary diseases, accompanied by the dysfunction of mitochondria and the disruption of cellular energy synthesis, that affect various tissues and organ systems. Mitochondrial DNA mutation-caused disorders are difficult to study because of the insufficient number of clinical cases and the challeng...

The theory of rheology of non-Newtonian fluids is based on the generalized Newtonian hypothesis of viscosity. The viscometers for non-Newtonian fluids should implement fluid flows with the known stress and strain state parameters distributions. Ideally, the distributions should be homogeneous in the flow domain. The idea of the proposed method is b...

Aggregation of alpha-synuclein (α-Syn) drives Parkinson’s disease (PD), although the initial stages of self-assembly and structural conversion have not been directly observed inside neurons. In this study, we tracked the intracellular conformational states of α-Syn using a single-molecule Förster resonance energy transfer (smFRET) biosensor, and we...

Chronic human diseases, especially age-related disorders, are often associated with chronic inflammation. It is currently not entirely clear what factors are responsible for the sterile inflammatory process becoming chronic in affected tissues. This process implies impairment of the normal resolution of the inflammatory response, when pro-inflammat...

Neurodegenerative disorders are currently incurable devastating diseases which are characterized by the slow and progressive loss of neurons in specific brain regions. Progress in the investigation of the mechanisms of these disorders helped to identify a number of genes associated with familial forms of these diseases and a number of toxins and ri...

The brain produces various reactive oxygen species in enzymatic and non-enzymatic reactions as a by-product of metabolism and/or for redox signaling. Effective antioxidant system in the brain cells maintains redox balance. However, neurons and glia from some brain regions are more vulnerable to oxidative stress in ischemia/reperfusion, epilepsy, an...

Brain is one of the most energy-demanding organs. Energy in the form of ATP is produced in brain cells predominantly in oxidative phosphorylation coupled to mitochondrial respiration. Any alteration of the mitochondrial metabolism or prolonged ischemic or anoxic conditions can lead to serious neurological conditions, including neurodegenerative dis...

Brain is one of the most energy-demanding organs. Energy in the form of ATP is produced in brain cells predominantly in oxidative phosphorylation coupled to mitochondrial respiration. Any alteration of the mitochondrial metabolism or prolonged ischemic or anoxic conditions can lead to serious neurological conditions, including neurodegenerative dis...

Brain is not homogenous and neurons from various brain regions are known to have different vulnerabilities to mitochondrial mutations and mitochondrial toxins. However, it is not clear if this vulnerability is connected to different energy metabolism in specific brain regions. Here, using live‐cell imaging, we compared mitochondrial membrane potent...

A device for optical biopsy with a fluorescence spectroscopy channel and a fine-needle optical probe for use in fine-needle aspiration biopsy of liver tumors is described. To test the developed device, experimental measurements of the fluorescence of internal organs of a laboratory rat were carried out in vivo while exposing the tissue surface to m...

The article describes the development of the optical phantom mimicking flavin adenine dinucleotide (FAD) and skin collagen fluorescence. The results of experimental studies using fluorescence imaging and fluorescence spectroscopy methods are demonstrated. The method of optical phantom production for quick calibration of fluorescence imaging devices...

The use of liposomal nanoparticles with an incorporated active substance is an innovative and promising approach to diagnostics and therapy. The application of liposomal nanoparticle-based drugs allows for targeted localized delivery, overcomes the natural barriers within the body effectively, and minimizes possible side effects. Liposomes are able...

The studies were carried out on groups of clinically healthy mice line of outbred CD-1 stock. The model animals were divided into 2 groups and received experimental liposomal formulations. Using the method of fluorescence spectroscopy, we investigated the effectiveness of penetration into the circulatory system of fluorescently stained liposomes wi...

The low-temperature fractionation of fruit Malus domestica Borkh (Common Antonovka) has been performed. We obtained by fractionation the biologically active products that are the dehydrated concentrate of juice and the powder of pomace fibers. Use of low temperature minimizes biological value losses during processing. These fractions of fruit Malus...

This study examines the effect of blood absorption on the endogenous fluorescence signal intensity of biological tissues. Experimental studies were conducted to identify these effects. To register the fluorescence intensity, the fluorescence spectroscopy method was employed. The intensity of the blood flow was measured by laser Doppler flowmetry. W...