Andreina Schoeberlein

Universität Bern | UniBe · Department for BioMedical Research (DBMR)

The role of reactive astrocytes in perinatal white matter injury (WMI) is unclear. In a mouse model of WMI, we provide evidence that impairing the formation of a C3-expressing neuroinflammatory reactive astrocyte sub-state rescues myelination and behavioral deficits. We further demonstrate the presence of C3-expressing reactive astrocytes in human...

Hyperuricemia is a common feature in pregnancies compromised by pre-eclampsia, a pregnancy disease characterized by hypertension and proteinuria. The role of uric acid in the pathogenesis of pre-eclampsia remains largely unclear. The aim of this study was to investigate the effect of elevated uric acid serum levels during pregnancy on maternal bloo...

The selection of an appropriate animal model is key to the production of results with optimal relevance to human disease. Particularly in the case of perinatal brain injury, a dearth of affected human neonatal tissue available for research purposes increases the reliance on animal models for insight into disease mechanisms. Improvements in obstetri...

Perinatal derivatives (PnD) are gaining interest as a source for cell-based therapies. Since the eye is easily accessible to local administration, eye diseases may be excellent candidates to evaluate novel therapeutic approaches. With this work, we performed a systematic review of published preclinical and clinical studies addressing PnD in the tre...

Perinatal tissues, such as placenta and umbilical cord contain a variety of somatic stem cell types, spanning from the largely used hematopoietic stem and progenitor cells to the most recently described broadly multipotent epithelial and stromal cells. As perinatal derivatives (PnD), several of these cell types and related products provide an inter...

The increasing cancer incidence has certified oncological management as one of the most critical challenges for the coming decades. New anticancer strategies are still needed, despite the significant advances brought to the forefront in the last decades. The most recent, promising therapeutic approaches have benefitted from the application of human...

The last 18 years have brought an increasing interest in the therapeutic use of perinatal derivatives (PnD). Preclinical studies used to assess the potential of PnD therapy include a broad range of study designs. The COST SPRINT Action (CA17116) aims to provide systematic and comprehensive reviews of preclinical studies for the understanding of the...

Failed or altered gliogenesis is a major characteristic of diffuse white matter injury in survivors of premature birth. The developmentally regulated long noncoding RNA (lncRNA) H19 inhibits S-adenosylhomocysteine hydrolase (SAHH) and contributes to methylation of diverse cellular components, such as DNA, RNA, proteins, lipids, and neurotransmitter...

Knowledge of the beneficial effects of perinatal derivatives (PnD) in wound healing goes back to the early 1900s when the human fetal amniotic membrane served as a biological dressing to treat burns and skin ulcerations. Since the twenty-first century, isolated cells from perinatal tissues and their secretomes have gained increasing scientific inte...

Peripartum cerebral hypoxia and ischemia, and intrauterine infection and inflammation, are detrimental for the precursor cells of the myelin-forming oligodendrocytes in the prematurely newborn, potentially leading to white matter injury (WMI) with long-term neurodevelopmental sequelae. Previous data show that hypomyelination observed in WMI is caus...

BACKGROUND Perinatal complications may result in life-long morbidities, among which cerebral palsy (CP) is the most severe motor disability. Once developed, CP is a non-progressive disease with a prevalence of 1-2 per 1000 live births in developed countries. It demands an extensive and multidisciplinary care. Therefore, it is a challenge for our he...

Perinatal brain injury (PBI) in preterm birth is associated with substantial injury and dysmaturation of white and gray matter, and can lead to severe neurodevelopmental deficits. Mesenchymal stromal cells (MSC) have been suggested to have neuroprotective effects in perinatal brain injury, in part through the release of extracellular vesicles like...

Background Preterm newborns are at high risk of developing neurodevelopmental deficits caused by neuroinflammation leading to perinatal brain injury. Human Wharton’s jelly mesenchymal stem cells (hWJ-MSC) derived from the umbilical cord have been suggested to reduce neuroinflammation, in part through the release of extracellular vesicle-like exosom...

OBJECTIVE: The overall hypomyelination of the brain, resulting from the disturbed development and differentiation of the myelin-forming oligodendrocytes, is a major hallmark of perinatal white matter injury (WMI). We have recently shown in an animal model of WMI that transplanted mesenchymal stem cells (MSC) were able to reverse hypomyelination, wh...

Objective Infants born prematurely are at high risk to suffer from perinatal white matter injury. Perinatal white matter injury is characterized by exacerbated neuroinflammation which causes subsequent neurodevelopmental deficits. In animal models of white matter injury, Wharton’s jelly mesenchymal stem/stromal cells (WJ-MSC) derived from umbilica...

The loss of oligodendrocyte progenitor cells leading to overall hypomyelination of the brain is a major hallmark in perinatal brain damage. Experimental transplantations of mesenchymal stem cells (MSC) in animal models of perinatal brain damage strongly indicate that the regenerative effects are based on released factors such as MSC-derived exosome...

Survivors of preterm birth are at risk to develop hypoxic-ischemic encephalopathy (HIE) of prematurity. HIE is characterized by severe neuroinflammation leading to severe long-term disability. In animal models of HIE, Wharton's jelly mesenchymal stem cells (WJ-MSC) derived from umbilical cords can reduce neuroinflammation, in part because they rele...

The loss of oligodendrocyte progenitor cells (OPC) is a hallmark of perinatal brain injury. Our aim was to develop an in vitro culture condition for human chorion-derived mesenchymal stem cells (MSC) that enhances their stem cell properties and their capability to differentiate towards OPC-like cells. MSC were grown either in serum replacement medi...

OBJECTIVE: Perinatal brain damage is accompanied by oligodendrocyte progenitor cell loss. The neuroregenerative effects of transplanted mesenchymal stem cells (MSC) in animal models of perinatal brain damage are presumed to rely on secreted factors such as MSC-derived extracellular vesicles (EV). Thus, the aim of this study is to evaluate the capac...

OBJECTIVE: Every year, an estimated 15 million babies are born preterm. Survivors of preterm birth are at risk to develop severe necrotizing enterocolitis, bronchopulmonary dysplasia and perinatal brain damage. The latter is caused by cerebral hypoxia-ischemia (HI) and systemic maternal-fetal inflammation and leads to severe neurological adverse ef...

Hypoxic-ischemic (HI) insult in the perinatal phase harbors a high risk of encephalopathy in the neonate. Brain cells undergo apoptosis, initiating neurodegeneration. So far, therapeutic approaches such as cooling remain limited. Transplantation of mesenchymal stem cells (MSCs) exhibits therapeutic success despite the short-time survival in the hos...

Background aims: Wharton's jelly mesenchymal stromal cells (WJ-MSCs) might be ideal candidates to treat perinatal brain damage. Their secretome has been shown to have beneficial effects on neuroregeneration, in part through interaction with neural progenitor cells (NPCs). However, it remains unclear whether cell-to-cell contact decisively contribu...

OBJECTIVE: We have previously shown that the transplantation of mesenchymal stem cells (MSC) in animal models of perinatal brain damage has neuro-regenerative effects in spite of low long-term survival in host tissue. Thus, the therapeutic effect of MSC may be due to secreted factors including MSC-derived exosomes. The aim of this study is the char...

The development of a mammalian brain is a complex and long-lasting process. Not surprisingly, preterm birth is the leading cause of death in newborns and children. Advances in perinatal care reduced mortality but morbidity still represents a major burden. New therapeutic approaches are thus desperately needed. Given that mesenchymal stem/stromal ce...

Preterm white matter injury (WMI) is an important cause for long-term disability. Stem cell transplantation has been proposed as a novel therapeutic approach. However, intracerebral transplantation is not feasible for clinical purpose in newborns. Intranasal delivery of cells to the brain might be a promising, non-invasive therapeutic approach to r...

Perinatal complications in both term- and preterm-born infants are a leading cause of neonatal morbidities and mortality. Infants face different challenges in the neonatal intensive care unit with long-term morbidities such as perinatal brain injury and bronchopulmonary dysplasia being particularly devastating. While advances in perinatal medicine...

The discovery of mesenchymal stem cells (MSCs) in perinatal sources, such as the amniotic fluid (AF) and the umbilical connective tissue, the so-called Wharton's jelly (WJ), has transformed them into promising stem cell grafts for the application in regenerative medicine. The advantages of AF-MSCs and WJ-MSCs over adult MSCs, such as bone marrow-de...

A synthetic peptide (sPIF) analogous to the mammalian embryo-derived PreImplantation Factor (PIF) enables neuroprotection in rodent models of experimental autoimmune encephalomyelitis and perinatal brain injury. The protective effects have been attributed, in part, to sPIF's ability to inhibit the biogenesis of microRNA let-7, which is released fro...

OBJECTIVE: New routes for cell transplantation into the brain need to be explored as intracerebral or intrathecal applications have a high risk to cause damage to the central nervous system. It has been hypothesized that transnasally administrated cells bypass the blood-brain barrier and migrate along the olfactory neural route into the brain and c...

Significance Secreted from viable embryos into the maternal circulation, PreImplantation factor (PIF) has long been implicated in modulating maternal immune tolerance and promoting embryo implantation. Recent evidence also suggests its possible therapeutic use in CNS disorders. However, the molecular signal-transduction pathways driving PIF-mediate...

Abstract Objective The aim of the study was to compare the neuroglial phenotype of Wharton's jelly derived mesenchymal stem cells (WJ-MSC) from pregnancies complicated with preeclampsia and gestational age (GA)-matched controls. Methods WJ-MSC were isolated from umbilical cords from both groups and analyzed for the cell surface expression of MSC ma...

OBJECTIVE: Premature birth is a major cause of neonatal morbidity and mortality. Although improvements in perinatal and neonatal care reduce mortality, morbidity remains a serious challenge. Sur-vivors of premature birth will confront enormous neurological problems. Transplantation of mesenchymal stem cells and treatment of ischemic brain injury ha...

Background: Premature birth is a major cause of neonatal morbidity and mortality. Although improvements in perinatal and neonatal care reduce mortality, morbidity remains a serious challenge. Survivors of premature birth will confront enormous neurological problems. Wharton's jelly-derived mesenchymal stem cells (WJ-MSC) are a promising source for...

Fragestellung: Perinataler Hirnschaden und Frühgeburtlichkeit können zu klinischen Problemen führen. Neurale Entwicklungsstörungen sind die Folge. Ziel dieser Studie ist es den therapeutischen Effekt der intrakraniellen Stammzelltransplantation von humanen mesenchymalen Stammzellen (MSC) zu testen. Die perinatale hypoxisch-ischämische Hirnschädigun...

Objective: The aim of the study is to determine the neuroglial differentiation potential of human Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) from preterm birth when compared to term delivery. Study design: The WJ-MSCs from umbilical cords of preterm birth and term controls were isolated and induced into neural progenitors. The cell...

OBJECTIVE: Perinatal brain damage is a major neurological problem in surviving premature infants. Transplantation experiments in various animal models suggest a neuro-regenerative potential of multipotent mesenchymal stem cells (MSC). The curative effect of MSC might be due to their production of neurotrophic factors. The Wharton's jelly represents...

OBJECTIVE: Peripartal brain injury in the premature infant leads to a large spectrum of clinical problems including lifelong neurodevelopmental deficits. We examined intracranial mesenchymal stem cells (MSC) transplantation as a promising therapy to facilitate motor recovery after perinatal hypoxic-ischemic brain damage with inflammatory component...

OBJECTIVE: 1% of newborns are affected by neurological injuries. Treating such complex diseases is difficult. Multipotent mesenchymal stem cells (MSC) have the ability to differentiate towards neuronal lineages with appropriate stimulation. Moreover, MSC are easily accessible in placenta and could be a valuable source as cell graft for preand perin...

OBJECTIVE: The aim of the study was to asses therapeutic effects of an early intracranial mesenchymal stem cell transplantation after induction of perinatal brain injury in an perinatal rat model. STUDY DESIGN: The study was conducted in a sham controlled design. Subcutaneous administration of LPS from E. coli and ligation of the left carotid arter...

Aim: Perinatal brain injury in the premature infant often leads to severe longterm disability. Stem cell transplantation has been proposed as a therapy for neurodegenerative diseases.The aim of this study is to assess the therapeutic potential of stem cell transplantation in an animal model of peripartal brain damage. Perinatal brain injury model:...

The aim of this study is to assess early homing of placenta-derived stem cells after perinatal intracerebral transplantation in rats. Neonatal Wistar rats (2-4 days old) were anesthetized, and 250,000 human placenta-derived mesenchymal stem cells (MSC) injected into the lateral ventricle or the paraventricular white matter using a stereotactic fram...

Kongressbeitrag Einleitung: Perinataler Hirnschaden besonders bei Frühgeburten kann häufig zu klinischen Problemen führen. Hypothermie ist derzeit die einzige therapeutische Option. Stammzelltransplantation zur Regeneration von geschädigten Hirngewebe ist eine vielversprechende Methode. Die Machbarkeit, Proliferation, Migration, und Integration der...

The objective of the study was to determine the feasibility of generating a biodegradable, stem cell-loaded osteogenic composite graft from human placenta. Initially, a scaffold from human chorion membrane was produced. Human placenta mesenchymal stem cells (MSCs) derived from either first-trimester chorionic villi or term chorion membrane were dif...

The Fetus as Recipient for Pre- and Perinatal Stem Cell Transplantation Prenatal Cell Transplantation: Current Experience How Do We Improve the Success of Prenatal Stem Cell Transplantation? Mesenchymal Stem Cells for Prenatal Transplantation Fetal Gene Therapy as a New Strategy Current Issues of Postnatal Gene Therapy Prenatal Gene Therapy Animal...

We aimed to induce neural stem (NSC) and progenitor cells (NPC) from human placental tissues. Placental stem cells from first-trimester placental chorionic villi and term chorion were isolated. Neural differentiation was initiated with plating on collagen, retinoic acid, and/or human brain-derived neurotrophic factor and epidermal and fibroblast gr...

Advances in human prenatal medicine and molecular genetics have allowed the diagnosis of many genetic diseases early in gestation. In-utero transplantation of allogeneic hematopoietic stem cells (HSC) has been successfully used as a therapy in different animal models and recently also in human fetuses. Unfortunately, clinical success of this novel...

RESULTS: A total of 80 women fit inclusion criteria. 51 women underwent delivery after a mature FLM-TDx test, and 29 after immature or indeterminate FLM-TDx testing with an LS 2.0. The risk of developing respiratory morbidity was significantly higher in the group delivered after reflex L/S testing (0% vs. 24%, p 0.001). There were 3 cases of RDS an...