Andrei Krasnopeev

Russian Academy of Sciences | RAS · Limnological Institute SB

For the first time, microcystin-producing cyanobacteria have been detected in Khubsugul, which is ancient, pristine and one of the world’s largest lakes. The microcystin synthetase genes belonged to the genera Nostoc, Microcystis and possibly Snowella spp. No microcystins were found in the water of the lake. Using the HPLC-HRMS/TOF, five microcysti...

Cyanobacterial expansion is harmful to the environment, the ecology of Lake Baikal and the economy of nearby regions and can be dangerous to people and animals. Since 2011, the process of colonisation of the lake with potentially toxic cyanobacteria belonging to the genus Tychonema has continued. An understanding of the mechanism of successful expa...

The gut microbiota and its development for the health of pets are currently being actively developed. The composition of the gut microbiome is found in the reduction of food intake, the state of the immune system of animals, productivity and growth in livestock content. Also found in the colon are unusual pathogen phyla that can serve as markers of...

This article characterises viral fraction metatranscriptomes (smaller than 0.2 µm) from the pelagic zone of oligotrophic Lake Baikal (Russia). The study revealed the dominance of transcripts of DNA viruses: bacteriophages and algal viruses. We identified transcripts similar to Pithovirus sibericum, a nucleocytoplasmic large DNA virus (NCLDV) isolat...

The ecological crisis in the coastal zone of Lake Baikal, which began in 2010-2011, caused changes in the taxonomic composition of microbial communities in the water and sponges. To identify these changes and their possible cause, we analyzed and compared the composition and diversity of microbial communities in the Baikal bacterioplankton and ende...

Lake Baikal phage communities are important for lake ecosystem functioning. Here we describe the diversity of T4-bacteriophage associated with the bacterial fraction of filtered water samples collected from the pelagic zone, coastal zone and shallow bays. Although the study of the diversity of phages for the g23 gene has been carried out at Lake Ba...

The diversity of aerobic anoxygenic phototrophs (AAPs) and rhodopsin-containing bacteria in the surface microlayer, water column, and epilithic biofilms of Lake Baikal was studied for the first time, employing pufM and rhodopsin genes, and compared to 16S rRNA diversity. We detected pufM-containing Alphaproteobacteria (orders Rhodobacterales, Rhizo...

Lake Sevan, Armenia, is the largest freshwater body in the Caucasus region. Cyanobacteria have become increasingly dominant in summer in Lake Sevan, reflecting the eutrophication of the lake and formed a massive bloom event in 2018. These recent observations mark the transition of this previously oligotrophic high mountain lake into a eutrophic lak...

This study investigates in detail species diversity of cyanobacteria which proliferated on the surface of diseased sponges and bedrock in Lake Baikal (Siberia, Russia) during the ecological crisis that has been progressing in the littoral zone since 2011 and caused numerous negative changes in biota. In 2014–2015, we examined samples from diseased...

This study describes two viral communities from the world’s oldest lake, Lake Baikal. For the analysis, we chose under-ice and late spring periods of the year as the most productive for Lake Baikal. These periods show the maximum seasonal biomass of phytoplankton and bacterioplankton, which are targets for viruses, including bacteriophages. At that...

We have assessed the diversity of bacteria near oil-methane (area I) and methane (area II) seeps in the pelagic zone of Lake Baikal using massive parallel sequencing of 16S rRNA, pmoA, and mxaF gene fragments amplified from total DNA. At depths from the surface to 100 m, sequences belonging to Cyanobacteria dominated. In the communities to a depth...

Here, we report on the sequences of the g23 communities of phages from biofilms and the surface microlayer in the most ancient lake of the world. The data are interpreted in terms of biogeography of the g23 communities. We show that bacteriophages from biotopes of biofilms of stones and sponges from the littoral zone and of the water from the pelag...

In this study, we analysed the diversity and composition of double-stranded DNA viral and bacterial communities within the sample of surface coastal water of Southern Baikal through metagenomics and deep sequencing of the 16S ribosomal RNA gene, respectively. The 16S rRNA gene analysis has revealed 14 phyla and dominance of the 'Actinobacteria' (43...

Characterization of the Genetic Diversity of T4-like Bacteriophages in Benthic Biofilms of Lake Baikal S. A. Potapov, A. Yu. Krasnopeev, I. V. Tikhonova, A. D. Galachyants, G. V. Podlesnaya, I. V. Khanaev, O. I. Belykh Limnological Institute SB RAS, Irkutsk Abstract. The genetic diversity of T4-like bacteriophages in biofilms formed on various subs...

Based on second generation sequencing (MiSeq platform), we determined the genetic diversity of T4-like bacteriophages of the family Myoviridae by analysing fragments of the major capsid protein gene g23 in the plankton of Lake Baikal. The sampling depth in our study was significantly higher than in those obtained by the Sanger method before. We obt...