Andreas Bosio

Andreas Bosio
Miltenyi Biotec B.V. & Co. KG | MACS · R&D

About

196
Publications
34,084
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
7,875
Citations

Publications

Publications (196)
Article
Major advances have been achieved in targeted immunotherapy with significant clinical benefits for patients. However, on-target/off-tumor toxicity is a major concern. This issue highlights the clinical need for better targets to improve the safety profile of immunotherapies. On-target/off-tumor toxicity is mainly based on the expression of tumor-as...
Article
Full-text available
Multimarker analysis can identify potential targets for immune therapy against solid tumors. With MICS we analysed human glioblastoma, ovarian and pancreatic carcinoma, and 16 healthy tissues, identifying the pair EPCAM/THY1 as a potential target for chimeric antigen receptor (CAR) T cell therapy for ovarian carcinoma. Using an Adapter CAR T cell a...
Article
Full-text available
While the transcription factor NEUROD2 has recently been associated with epilepsy, its precise role during nervous system development remains unclear. Using a multi-scale approach, we set out to understand how Neurod2 deletion affects the development of the cerebral cortex in mice. In Neurod2 KO embryos, cortical projection neurons over-migrated, t...
Article
Full-text available
The Human Developmental Cell Atlas (HDCA) initiative, which is part of the Human Cell Atlas, aims to create a comprehensive reference map of cells during development. This will be critical to understanding normal organogenesis, the effect of mutations, environmental factors and infectious agents on human development, congenital and childhood disord...
Preprint
Full-text available
Many critical advances in research utilize techniques that combine high-resolution with high-content characterization at the single cell level. We introduce the MICS (MACSima TM Imaging Cyclic Staining) technology, which enables the immunofluorescent imaging of hundreds of protein targets across a single specimen at sub-cellular resolution. MICS is...
Article
Full-text available
Shwachman–Diamond syndrome (SDS) is a bone marrow failure (BMF) syndrome associated with an increased risk of myelodysplasia and leukemia. The molecular mechanisms of SDS are not fully understood. We report that primitive hematopoietic cells from SDS patients present with a reduced activity of the small RhoGTPase Cdc42 and concomitantly a reduced f...
Article
Full-text available
The formation of the cerebellum is highly coordinated to obtain its characteristic morphology and all cerebellar cell types. During mouse postnatal development, cerebellar progenitors with astroglial-like characteristics generate mainly astrocytes and oligodendrocytes. However, a subset of astroglial-like progenitors found in the prospective white...
Article
Full-text available
A major roadblock prohibiting effective cellular immunotherapy of pancreatic ductal adenocarcinoma (PDAC) is the lack of suitable tumor-specific antigens. To address this challenge, here we combine flow cytometry screenings, bioinformatic expression analyses and a cyclic immunofluorescence platform. We identify CLA, CD66c, CD318 and TSPAN8 as targe...
Article
Full-text available
It has been over 30 years since visionary scientists came up with the term “Gene Therapy,” suggesting that for certain indications, mostly monogenic diseases, substitution of the missing or mutated gene with the normal allele via gene addition could provide long-lasting therapeutic effect to the affected patients and consequently improve their qual...
Conference Paper
The in-depth characterization of tumor-infiltrating leukocytes (TILs) is crucial to further improve cancer immunotherapies. Since TILs generally constitute a small subpopulation of solid tumors, they can be lost in the background noise of downstream analyses such as flow cytometry or single cell sequencing. Therefore, improving pre-enrichment metho...
Conference Paper
Glioblastoma multiforme (GBM) is a highly malignant, non-curative tumor of the primary central nervous system with dismal prognosis. Treatment options are limited and conventional therapies, including surgery, radiotherapy, chemotherapy have not resulted in major improvements in the survival of patients. The advent of chimeric antigen receptor (CAR...
Conference Paper
Glioblastoma multiforme (GBM), a highly malignant, incurable brain tumor of the primary central nervous system, was subclassified into different subsets. Here, we introduce the new MACSimaTM technology that uses iterative fluorescent staining, image acquisition, and signal erasure with multiple fluorochrome-conjugated antibodies per cycle. We have...
Chapter
The phenotype of a cell is determined by the amount, the proportion and the condition of proteins present in this cell. Although every cell in an organism possesses the same genetic information, only certain genes are transcribed into MESSENGER RIBONUCLEIC ACID (mRNA) according to the function and demands of the cell. Based on the information provi...
Conference Paper
The recent successes in gene therapy for the treatment of rare blood disorders such as primary immunodeficiencies and hemoglobinopathies have highlighted the need to generate robust and scalable manufacturing processes. Here we compare large scale lentiviral transductions of hemopoietic stem cells performed on the CliniMACS Prodigy® to manual proce...
Article
Full-text available
Fibroblasts are thought to be key players in the tumor microenvironment. Means to identify and isolate fibroblasts as well as an understanding of their cancer-specific features are essential to dissect their role in tumor biology. To date, the identification of cancer-associated fibroblasts is widely based on generic markers for activated fibroblas...
Conference Paper
Glioblastoma multiforme (GBM), a highly malignant, non-curative brain tumor of the primary central nervous system, has been subclassified in the past years in several distinct subtypes using a multitude of analysis methods. Here, we introduce the MACSima™ imaging platform which allows for fully automated, multiparametric, cyclic immunofluorescence...
Conference Paper
Here we report the use of the MACSima™ imaging platform to perform high-content imaging for the pre-clinical validation of tumor target expression on tumor and healthy human tissues indicative for potential on-target/off-tumor toxicity in vivo. Major advances have been achieved in cancer therapy in the past decade. In particular, targeted-immunothe...
Conference Paper
Cancer cell lines are widely used as in vitro models to study tumor biology and for efficacy testing of novel anti-cancer therapeutics. In the past, most of this work has been done using established cell lines that have been cultured for decades. Extensive in vitro propagation has been shown to lead to the acquisition of multiple genetic and epigen...
Conference Paper
Here we report the use of the MACSima™ imaging platform to perform high-content imaging for the pre-clinical validation of tumor target expression on tumor and healthy human tissues indicative for potential on-target/off-tumor toxicity in vivo. Major advances have been achieved in cancer therapy in the past decade. In particular, targeted-immunothe...
Conference Paper
Glioblastoma multiforme (GBM), a highly malignant, non-curative brain tumor of the primary central nervous system, has been subclassified in the past years in several distinct subtypes using a multitude of analysis methods. Here, we introduce the MACSima™ imaging platform which allows for fully automated, multiparametric, cyclic immunofluorescence...
Conference Paper
Background & Aim Human pluripotent stem cells (hPSCs) hold great promise for disease modeling, drug discovery and clinical applications. Therefore, working with highly pluripotent, well characterized and quality controlled cell stocks during development is crucial to ensure reproducible experimental conditions. Methods, Results & Conclusion Here,...
Conference Paper
Background & Aim Human mesenchymal stem cells (MSCs) hold great promise for clinical use and cell therapy applications and can be isolated from multiple tissue, e.g. bone marrow (BM), umbilical cord (UC) or adipose tissue (AT). As there is a high number of clinical trials and applications using MSCs, quality and safety of the resulting cellular pro...
Conference Paper
Background & Aim Major complications of allogeneic stem cell transplantation are GvHD and infections. T cell depletion (TCD) can effectively prevent GVHD, however it may prolong immune reconstitution and increase the risk of infections. Depletion of TCRab⁺ T cells combined with the transfer of CD45RA⁻ donor memory T cells can ameliorate the infecti...
Poster
Cancer cell lines are used to study tumor biology and as models for testing of novel anti-cancer therapeutics. The vast majority of this work has been done using established cell lines that have been cultured for decades. Upon extensive in vitro propagation, cancer cell lines acquire multiple genetic and epigenetic alterations, lose initial heterog...
Conference Paper
Full-text available
Introduction Syngeneic mouse tumour models are widely used to analyse tumour immunology due to their fully competent immune-repertoire and have paved the way for novel immunotherapy agents in multiple tumour entities. However, the amount and composition of tumour infiltrating leukocytes (TIL) is highly variable. This complicates targeted analysis,...
Article
The intestine is maintained by stem cells located at the base of crypts and distinguished by the expression of LGR5. Genetically engineered mouse models have provided a wealth of information about intestinal stem cells, while less is known about human intestinal stem cells due to difficulty detecting and isolating these cells. We established an org...
Preprint
Full-text available
The intestine is maintained by stem cells located at the base of crypts and distinguished by the expression of LGR5. Genetically engineered mouse models have provided a wealth of information about intestinal stem cells, while less is known about human intestinal stem cells due to difficulty detecting and isolating these cells. We established an org...
Article
Full-text available
In vivo brain electroporation of DNA expression vectors is a widely used method for lineage and gene function studies in the developing and postnatal brain. However, transfection efficiency of DNA is limited and adult brain tissue is refractory to electroporation. Here we present a systematic study of mRNA as a vector for acute genetic manipulation...
Article
Full-text available
In the postnatal forebrain regionalized neural stem cells along the ventricular walls produce olfactory bulb (OB) interneurons with varying neurotransmitter phenotypes and positions. To understand the molecular basis of this region specific variability we analyzed gene expression in the postnatal dorsal and lateral lineages in mice of both sexes fr...
Data
Table S1. Antibodies Used for Flow Cytometric Marker Screening and Standard Flow Cytometric Analyses
Data
Document S1. Supplemental Experimental Procedures, Figures S1–S5, Tables S2, S4, and S5
Data
Table S3. List of Top 100 Differentially Expressed Genes from Microarray Analysis of IAP+ (d16) versus IAP− (d16) and IAP+ (d16) versus IAP+ (d50)
Article
Full-text available
Human pluripotent stem cell (hPSC)-derived mesencephalic dopaminergic (mesDA) neurons can relieve motor deficits in animal models of Parkinson's disease (PD). Clinical translation of differentiation protocols requires standardization of production procedures, and surface-marker-based cell sorting is considered instrumental for reproducible generati...
Poster
Immunotherapy against cancer has proven clinical efficacy and tremendous potential in multiple tumor entities. Syngeneic mouse tumor models represent the gold standard to analyze effects of immunotherapy due to their fully competent immune-repertoire. However, the amount and composition of tumor infiltrating leukocytes (TIL) is highly variable, com...
Article
Aneuploidy is a hallmark of most human tumors, but the molecular physiology of aneuploid cells is not well characterized. In this study, we screened cell surface biomarkers of ~300 proteins by multiparameter flow cytometry using multiple aneuploid model systems such as cell lines, patient samples and mouse models. Several new biomarkers were identi...
Article
Astrocytes are the most abundant cell type of the central nervous system and cover a broad range of functionalities. We report here the generation of a novel monoclonal antibody, anti-astrocyte cell surface antigen-2 (Anti-ACSA-2). Flow cytometry, immunohistochemistry and immunocytochemistry revealed that Anti-ACSA-2 reacted specifically with a not...
Article
Medium spiny neurons (MSNs) are a key population in the basal ganglia network, and their degeneration causes a severe neurodegenerative disorder, Huntington's disease. Understanding how ventral neuroepithelial progenitors differentiate into MSNs is critical for regenerative medicine to develop specific differentiation protocols using human pluripot...
Article
Full-text available
Scientific Reports 6 : Article number: 35729; 10.1038/srep35729 published online: 21 October 2016 ; updated: 21 December 2016 . The original version of this Article contained a typographical error in the spelling of the author Nathalie Coré, which was incorrectly given as Coré Nathalie.
Poster
Full-text available
Tissue dissociation and preparation of single-cell suspensions with high cell viability and a minimum of cell debris are prerequisites for reliable cellular analysis, cell culture, and cell separation. As dissociation of adult brain requires sophisticated mechanical and enzymatic treatment to successfully disaggregate the tightly connected neural c...
Article
Full-text available
During neurogenesis, generation, migration and integration of the correct numbers of each neuron sub-type depends on complex molecular interactions in space and time. MicroRNAs represent a key control level allowing the flexibility and stability needed for this process. Insight into the role of this regulatory pathway in the brain is still limited....
Article
Gene modulation of human haematopoietic stem and progenitor cells (HSPCs) harbours great potential for therapeutic application of these cells and presents a versatile tool in basic research to enhance our understanding of HSPC biology. However, stable genetic modification might be adverse, particularly in clinical settings. Here, we review a broad...
Article
Full-text available
The use of in vitro cell line models for cancer research has been a useful tool. However, it has been shown that these models fail to reliably mimic patient tumors in different assays¹. Human tumor xenografts represent the gold standard with respect to tumor biology, drug discovery, and metastasis research²⁻⁴. Tumor xenografts can be derived from d...
Article
Full-text available
Extracellular vesicles (EVs) are specifically loaded with nucleic acids, lipids, and proteins from their parental cell. Therefore, the constitution of EVs reflects the type and status of the originating cell and EVs in melanoma patient’s plasma could be indicative for the tumor. Likewise, EVs might influence tumor progression by regulating immune r...
Conference Paper
Full-text available
Owing to their capacity to eradicate tumors, T cells represent an attractive means for immunomodulation in cancer immunotherapy. In this context, chimeric antigen receptor (CAR) – based therapies are receiving increasing attention. The combination of antibody-derived specificity with T cell effector function renders the immune cells MHC-independent...
Poster
Full-text available
The need for xeno-free and GMP-compliant production of clinical-scale mesenchymal stem cells (MSCs) is rising. A number of clinical Phase II/ III trials are registered. In this context, the use of fetal calf serum (FCS) is undesirable with regard to adverse events and lot-to-lot variations. StemMACS™ MSC Expansion Media Kit XF (StemMACS MSC XF) sup...
Chapter
Starting from a zygote, an organism is made up of thousands, highly organized stem cells, progenitor cells and postmitotic cells which are generated in spatio-temporally coordinated proliferation and differentiation steps. The ongoing advancements in cell culture, isolation techniques, and molecular analyses have driven our basic understanding of d...
Article
We report that the efficiency of reprogramming human somatic cells to induced pluripotent stem cells (hiPSCs) can be dramatically improved in a microfluidic environment. Microliter-volume confinement resulted in a 50-fold increase in efficiency over traditional reprogramming by delivery of synthetic mRNAs encoding transcription factors. In these sm...
Article
Full-text available
The surface protein composition of extracellular vesicles (EVs) is related to the originating cell and may play a role in vesicle function. Knowledge of the protein content of individual EVs is still limited because of the technical challenges to analyse small vesicles. Here, we introduce a novel multiplex bead-based platform to investigate up to 3...
Data
A novel multiplex bead-based platform highlights the diversity of extracellular vesicles
Chapter
MicroRNAs, or miRNAs, are key regulators of neural stem cell biology, controlling many important biophysiological and pathophysiological processes, including normal stem cell differentiation and cancer stem cell formation, renewal, and differentiation. The absence of miRNAs in neural stem cells has been shown to prevent numerous aspects of neural d...
Article
Full-text available
Rationale: Central questions such as cardiomyocyte subtype emergence during cardiogenesis or the availability of cardiomyocyte subtypes for cell replacement therapy require selective identification and purification of atrial and ventricular cardiomyocytes. However, current methodologies do not allow for a transgene-free selective isolation of atri...
Data
Intracellular and surface marker expression of manually separated and dissociated atria and ventricles. (A) Density plots show representative flow analysis of the cardiomyocyte content in both fractions as indicated by α-actinin staining. (B) Identity of atrial and ventricular cells was analyzed by qRT-PCR with regard to transcript levels of the ve...