Andreas Aufschnaiter

Andreas Aufschnaiter
Stockholm University | SU · Department of Biochemistry and Biophysics

Dr. rer. nat.

About

25
Publications
7,258
Reads
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315
Citations
Additional affiliations
December 2019 - present
Stockholm University
Position
  • PostDoc Position
April 2019 - November 2019
Stockholm University
Position
  • PostDoc Position
January 2019 - March 2019
Karl-Franzens-Universität Graz
Position
  • Researcher
Education
August 2015 - August 2018
Karl-Franzens-Universität Graz
Field of study
  • Molecular Biology and Biochemistry
October 2013 - July 2015
Graz University of Technology
Field of study
  • Biochemistry and Molecular Biomedical Sciences
October 2011 - January 2019
Medical University of Graz
Field of study
  • Human Medicine

Publications

Publications (25)
Article
Full-text available
Respiratory chains are crucial for cellular energy conversion and consist of multi-subunit complexes that can assemble into super-complexes. These structures have been intensively characterized in various organisms, but their physiological roles remain unclear. Here, we elucidate their function by leveraging a high-resolution structural model of ye...
Article
Full-text available
Mitochondria contain their own gene expression systems, including membrane-bound ribosomes dedicated to synthesizing a few hydrophobic subunits of the oxidative phosphorylation (OXPHOS) complexes. We used a proximity-dependent biotinylation technique, BioID, coupled with mass spectrometry to delineate in baker’s yeast a comprehensive network of fac...
Article
Full-text available
Mitochondrial dysfunction is a common feature of many neurodegenerative diseases, including proteinopathies such as Alzheimer’s or Parkinson’s disease, which are characterized by the deposition of aggregated proteins in the form of insoluble fibrils or plaques. The distinct molecular processes that eventually result in mitochondrial dysfunction dur...
Article
Full-text available
Mitochondrial dysfunction is a prominent trait of cellular decline during aging and intimately linked to neuronal degeneration during Parkinson's disease (PD). Various proteins associated with PD have been shown to differentially impact mitochondrial dynamics, quality control and function, including the leucine-rich repeat kinase 2 (LRRK2). Here, w...
Article
Full-text available
Intrinsic apoptosis as a modality of regulated cell death is intimately linked to permeabilization of the outer mitochondrial membrane and subsequent release of the protein cytochrome c into the cytosol, where it can participate in caspase activation via apoptosome formation. Interestingly, cytochrome c release is an ancient feature of regulated ce...
Article
Full-text available
A key function of mitochondria consists of energy conversion, performed with the help of the respiratory chain and the ATP synthase. Biogenesis of these essential molecular machines requires expression of nuclear and mitochondrially encoded genes. We describe our current understanding how these processes are coordinated and how they are organized i...
Article
Full-text available
The capacity of a cell to maintain proteostasis progressively declines during aging. Virtually all age-associated neurodegenerative disorders associated with aggregation of neurotoxic proteins are linked to defects in the cellular proteostasis network, including insufficient lysosomal hydrolysis. Here, we report that proteotoxicity in yeast and Dro...
Article
Full-text available
Nutrient limitation results in an activation of autophagy in organisms ranging from yeast, nematodes and flies to mammals. Several evolutionary conserved nutrient-sensing kinases are critical for efficient adaptation of yeast cells to glucose, nitrogen or phosphate depletion, subsequent cell-cycle exit and the regulation of autophagy. Here, we demo...
Preprint
Full-text available
Cytochrome c oxidase is a pivotal enzyme of the mitochondrial respiratory chain, which sustains bioenergetics of eukaryotic cells. Cox12, a peripheral subunit of cytochrome c oxidase, is required for full activity of the enzyme, but its exact function is unknown. Here, experimental evolution of a Saccharomyces cerevisiae Δ cox12 strain for ~300 gen...
Article
Full-text available
In all eukaryotic cells, intracellular organization and spatial separation of incompatible biochemical processes is established by individual cellular subcompartments in form of membrane-bound organelles. Virtually all of these organelles are physically connected via membrane contact sites (MCS), allowing interorganellar communication and a functio...
Article
Full-text available
Natural products represent important sources for the discovery and design of novel drugs. Bee venom and its isolated components have been intensively studied with respect to their potential to counteract or ameliorate diverse human diseases. Despite extensive research and significant advances in recent years, multifactorial diseases such as cancer,...
Chapter
Neuronal dysfunction during sporadic and familial forms of Parkinson's disease is intimately connected to mitochondrial dysfunction. Diverse genetic and environmental factors contributing to Parkinson's disease development and progression have been shown to interfere with and to compromise mitochondrial bioenergetics, dynamics and trafficking. Mito...
Article
Full-text available
Autophagy, a membrane-dependent catabolic process, ensures survival of aging cells and depends on the cellular energetic status. Acetyl-coenzyme A carboxylase 1 (Acc1) connects central energy- to lipid metabolism and is rate limiting for the de novo synthesis of lipids. However, it is unclear how de novo lipogenesis and its metabolic consequences a...
Article
Full-text available
Cellular ageing results in accumulating damage to various macromolecules and the progressive decline of organelle function. Yeast vacuoles as well as their counterpart in higher eukaryotes, the lysosomes, emerge as central organelles in lifespan determination. These acidic organelles integrate enzymatic breakdown and recycling of cellular waste wit...
Article
Full-text available
The dissemination of multi-resistant bacteria represents an enormous burden on modern health-care. Plasmid-borne conjugative transfer is the most prevalent mechanism, requiring a type IV secretion system that enables bacteria to spread beneficial traits, such as resistance to last-line antibiotics, among different genera. Inc18 plasmids, like the G...
Article
Full-text available
The accumulation of protein aggregates in neurons is a typical pathological hallmark of the motor neuron disease amyotrophic lateral sclerosis (ALS) and of frontotemporal dementia (FTD). In many cases, these aggregates are composed of the 43 kDa TAR DNA-binding protein (TDP 43). Using a yeast model for TDP 43 proteinopathies, we observed that the v...
Article
TDP-43 is a nuclear RNA-binding protein whose cytoplasmic accumulation is the pathological hallmark of amyotrophic lateral sclerosis (ALS). For a better understanding of this devastating disorder at the molecular level, it is important to identify cellular pathways involved in the clearance of detrimental TDP-43. Using a yeast model system, we syst...
Article
Full-text available
Cellular homeostasis requires a tightly controlled balance between protein synthesis, folding and degrada- tion. Especially long-lived, post-mitotic cells such as neurons depend on an e cient proteostasis system to maintain cellular health over decades. us, a functional decline of processes contributing to protein degra- dation such as autophagy an...
Article
Full-text available
The degeneration of dopaminergic neurons during Parkinson’s disease (PD) is intimately linked to malfunction of α-synuclein (αSyn), the main component of the proteinaceous intracellular inclusions characteristic for this pathology. The cytotoxicity of αSyn has been attributed to disturbances in several biological processes conserved from yeast to h...
Article
Conjugative transfer plays a major role in the transmission of antibiotic resistance in bacteria. pIP501 is a Gram-positive conjugative model plasmid with the broadest transfer host-range known so far and is frequently found in Enterococcus faecalis and Enterococcus faecium clinical isolates. The pIP501 type IV secretion system is encoded by 15 tra...
Article
Full-text available
Peroxisomes and mitochondria are highly dynamic organelles with diverse roles in cellular signaling and metabolism. Oxidative metabolism and in particular the production and detoxification of reactive oxygen species (ROS) link peroxisomes and mitochondria to cell death and aging. Interestingly, peroxisomes and mitochondria exist in close functional...