Andrea Zaccagnini

• University of Buenos Aires

Objective To evaluate if variation in the end‐tidal CO2 partial pressure (∆Petco2) after a fluid challenge could predict fluid responsiveness with a sensitivity of 75% and a specificity of 70% in healthy anesthetized and mechanically ventilated dogs. Design Diagnostic accuracy study. Setting University hospital. Animals Twenty‐seven dogs admitte...

Objective To evaluate the changes in flow velocities of the middle cerebral artery before and after a carotid compression maneuver using transcranial color‐coded duplex sonography (TCCD) in healthy anesthetized dogs under mechanical ventilation. Design Prospective study. Setting University teaching hospital. Animals Eleven healthy adult dogs. I...

This study aimed to evaluate whether the velocity-time integral of aortic blood flow (VTIAo) assessed by transthoracic echocardiography is significantly increased after passive leg-raising maneuver (PLRM) in anesthetized and mechanically ventilated fluid responder pigs. Eight healthy Landrace pigs were anesthetized, mechanically ventilated, and sub...

Genome editing in pigs for xenotransplantation has seen significant advances in recent years. This study compared three methodologies to generate gene-edited embryos, including co-injection of sperm together with the CRISPR-Cas9 system into oocytes, named ICSI-MGE (mediated gene editing); microinjection of CRISPR-Cas9 components into oocytes follow...

The current COVID-19 pandemic has led the world to an unprecedented global shortage of ventilators, and its sharing has been proposed as an alternative to meet the surge. This study outlines the performance of a preformed novel interface called ’ACRA’, designed to split ventilator outflow into two breathing systems. The ’ACRA’ interface was built u...

Objectives To evaluate the use of 0.7 mA as a fixed electrical current to indicate epidural needle placement and to confirm that 0.7 mA is greater than the upper limit of the minimal electrical threshold (MET) for sacrococcygeal epidural needle placement in dogs. Study design Prospective clinical study. Animals A group of 20 client-owned dogs. M...

Objective To describe the sonoanatomy of the abdominal wall in live cats and to compare the distribution pattern of two versus three ultrasound-guided transversus abdominis plane (TAP) injections using clinically applicable volumes of lidocaine-dye solution in cat cadavers. Study design Prospective anatomical study. Animals A total of eight clien...

Nerve stimulation (NS) and ultrasound have been reported as useful tools to determine real-time epidural injection in cats (Otero et al 2016). The aim of the study was to determine if a nerve stimulation test (NST) could act as a monitoring technique to confirm sacrococcygeal epidural needle placement in dogs. Eight adult Beagle dogs (14 ± 2.5 kg)...

Objectives: To describe the ultrasonographic anatomy of the caudal lumbar spine in cats and to detect ultrasound (US) signs associated with epidural or intrathecal injection. Study design: Prospective, clinical study. Animals: Twenty-six client-owned cats. Methods: Transverse (position 1) and parasagittal (position 2) two-dimensional US scan...

Objective To determine if a nerve stimulation test (NST) could act as a monitoring technique to confirm sacrococcygeal epidural needle placement in cats.Study designProspective experimental trial in a clinical setting.AnimalsTwenty-four adult cats, scheduled for a therapeutic procedure where epidural anesthesia was indicated.Methods Under general a...

Objective To determine the minimal electrical threshold (MET) necessary to elicit muscle contraction of the pelvic limb or tail when an insulated needle is positioned outside (METout) and inside (METin) the lumbosacral epidural space in cats. Study designProspective, blinded study. AnimalsTwelve mixed-breed healthy adult cats, scheduled for a thera...

To determine the minimal electric threshold of neurostimulation dorsally and ventrally to the interarcuate ligament in the lumbosacral area necessary to cause muscle contraction of the hind limb or tail and determine whether a continuous electrical stimulation applied to an insulated needle during lumbosacral epidural needle placement could be used...