Andrea Pilloni

Sapienza University of Rome | la sapienza · Department of Oral and Maxillo-facial Sciences

Background: Advanced platelet-rich fibrin (A-PRF) is produced by centrifuging the patient’s blood in vacuum tubes for 14 min at 1500 rpm. The most important component of A-PRF is the platelets, which release growth factors from their ⍺-granules during the clotting process. This process is believed to be the main source of growth factors. The aim of...

Aim: To assess the differential clinical response to step 2 of periodontal therapy and repeated subgingival instrumentation between teeth with suprabony and intrabony defects. Methods: Electronic and manual search were performed to identify studies reporting the differential clinical outcomes of non-surgical periodontal therapy (NSPT) in presenc...

Background The coronally advanced flap (CAF) can be a predictable surgical technique for the treatment of gingival recessions. However, the characteristics of the defect (e.g., limited amount of keratinized gingiva or flap tension, etc.) may limit the use of the CAF with a possible requirement of additional surgical interventions (i.e., the use of...

Purpose: To evaluate the response of human peri-implant soft tissue (PIST) on different healing abutment materials 24 hours after positioning, by assessing the expression of genes related to the early connective tissue wound healing response. Materials and methods: Experimental abutments of 4 different materials (A): grade 4 titanium, (B) grade...

Polynucleotides and Hyaluronic Acid (PN-HA) mixture showed several effects in modulation of healing process. The aim of this study was to assess the safety and clinical performance of PN-HA alone or in association with Deproteinized Bovine Bone Mineral (DBBM) with papillary preservation flaps (PPF) in the treatment of residual pockets. A total of 4...

The purpose of this study was to evaluate the clinical efficacy of a non-surgical periodontal treatment using a piezoelectric power-driven device with a novel insert. Plaque index (PlI), bleeding on probing (BoP), probing depth (PD), recession depth (Rec) and clinical attachment level (CAL) were assessed at 6 weeks, 3 months and 6 months. Furthermo...

Dental plaque is a biofilm structured in an extracellular matrix of polymers of host and microbial origin; the microorganisms can coexist in harmony with the host, thus guarantying oral health. Environmental modifications can lead to dysbiosis and onset of oral diseases; in fact, plaque is the etiological agent both of periodontal disease and denta...

Background: The purpose of this study was to evaluate the reliability and accuracy in the assignment of the case definitions of peri-implant health and diseases according to the 2018 Classification of Periodontal and Peri-implant Diseases and Conditions. Methods: Ten undergraduate students, 10 general dentists, and 10 experts in implant dentistry p...

(1) Background: Peri-implantitis is a multi-factorial disease with an inflammatory background that occurs in both soft and hard tissues surrounding implants. In recent years, the understanding of the cellular, molecular and genetic background of peri-implantitis has broadened. This study aims to summarize the currently available articles on the sub...

Background: Hyaluronic acid (HA) exerts a fundamental role in tissue repair. In vitro and animal studies demonstrated its ability to enhance wound healing. Nevertheless, in vivo human studies evaluating mechanisms involved in oral soft tissue repair are lacking. The aim of this study was to evaluate the in vivo effect of HA on early wound healing o...

Background: The aims of this study are to retrospectively compare the clinical, radiographic and esthetic outcomes of platform switching (PS) and platform matching (PM) dental implants in the anterior maxilla after ≥ 10 years of functional loading. Methods: Marginal bone loss (MBL) levels were recorded; furthermore, peri-implant clinical parameters...

Polynucleotides (PN) and hyaluronic acid (HA) have been effective in stimulating the growth of primary gingival fibroblasts and promoting wound healing. The aim of this study was to investigate the clinical efficacy of a gel containing PN and HA used in association with subgingival re-instrumentation in the treatment of residual periodontal pockets...

Previous systematic reviews have reported that coronally advanced flap (CAF) + connective tissue graft (CTG) are the gold standard in root coverage procedures (RCP). Nevertheless, adjunctive treatment with hyaluronic acid (HA) has been proposed to aim at improving clinical outcomes and reducing patient morbidity. The aim of this systematic review a...

Objectives: The purpose of this study was to assess the diagnostic accuracy and the inter-rater agreement among general dentists when staging and grading periodontitis cases with the aid of a software application (SA) developed by the Italian Society of Periodontology and Implantology. Materials and methods: Ten general dentists were asked to in...

Background Several data have demonstrated the occurrence of erosive arthritis in Systemic Lupus Erythematosus (SLE) patients. However, few studies have focused on the pathogenic mechanisms involved in this feature. The implication of oral pathogens has been proved in Rheumatoid Arthritis: in particular, Porphyromonas gingivalis (Pg), by inducing ci...

Background: Several data have demonstrated the occurrence of erosive arthritis in Systemic Lupus Erythematosus (SLE) patients. However, a few studies have focused on the pathogenic mechanisms involved in this feature. The implication of oral pathogens has been proved in Rheumatoid Arthritis: in particular, Porphyromonas gingivalis (Pg), by inducin...

Chlorhexidine digluconate (CHX) is considered the gold standard for oral cavity antiseptic treatment. Nevertheless, several in vitro studies have reported detrimental effects in oral tissue repair. The aim of the present study was to evaluate the in vivo effect of post-surgical CHX mouth rinse on gingival tissue (G) 24 h after injury. G biopsies we...

Objectives: Peri-implant tissue maintenance and treatment is becoming a serious challenge in implantology. With increasing numbers of implants being placed, more cases of peri-implant mucositis and peri-implantitis is seen. A digital survey on peri-implant disease management was issued to experts in periodontology and implantology to identify the t...

The aim of this case series was to present the clinical outcomes of non-contained intrabony periodontal defects (IPDs) treated by means of papillary preservation flaps in association with a slowly resorbable bovine pericardium membrane (BPM) and a low-temperature-treated bovine bone graft (BBG). Eight healthy, non-smoking patients (two males and si...

Objectives The aim of this randomized controlled clinical trial was to compare the clinical outcomes obtained in intrabony defects following regenerative periodontal surgery using the single-flap approach (SFA) in conjunction with either hyaluronic acid (HA) or enamel matrix derivative (EMD). Materials and methods Thirty-two intrabony defects in 3...

The aim of the present study was to evaluate the adjunctive effect of hyaluronic acid (HA) gel in the treatment of residual periodontal pockets over a 12-month period. Periodontal patients presenting at least one residual periodontal pocket 5–9 mm of depth in the anterior area were recruited from six university-based centers. Each patient was rando...

The purpose of this study was to histologically examine the clinically healthy gingiva of patients with altered passive eruption (APE). Five patients with type 1 APE were enrolled. They underwent scaling and polishing and received oral hygiene instructions. After 6 months of supervised plaque control and uninterrupted gingival clinical health (Ging...

Aim: To investigate the potential benefit of enamel matrix derivative (EMD) as adjunct to re-instrumentation of residual pockets persisting after steps 1 and 2 of periodontal therapy. Material & methods: 44 adult patients participated in a multicenter feasibility randomized clinical trial with split-mouth design. They had presented at re-evaluat...

Aim: Our aim was to evaluate gene expression profiling of fibroblasts from human alveolar mucosa (M), buccal attached gingiva (G) and palatal (P) tissues during early wound healing, correlating it with clinical response. Materials and Methods: M, G and P biopsies were harvested from six patients at baseline and 24 hrs after surgery. Clinical respon...

Objectives: In-vitro data have shown that cross-linked hyaluronic acid (HA) enhances the proliferative and migratory properties of cells involved in periodontal wound healing/regeneration, stabilizes the blood clot, reduces the inflammatory response, and facilitates angiogenesis. The aim of this study was to histologically evaluate the effects of...

Aim: The objective of this study was to evaluate consistency and accuracy of the periodontitis staging and grading classification system. Methods: Thirty participants (10 periodontal experts, 10 general dentists and 10 undergraduate students) and a gold-standard examiner were asked to classify 25 fully documented periodontitis cases twice. Fleis...

Background: Aim of the present study was to ascertain if a combination of leukocyte and platelet-rich fibrin (L-PRF) + autogenous bone graft (ABG) may be a clinically "non-inferior" treatment modality as compared with the association of Enamel Matrix Derivative (EMD) with ABG in the management of intrabony defects (IBDs). Methods: Forty-four pat...

BACKGROUND: Root trunk length (RTL) plays an important role in the diagnosis, prognosis and treatment of periodontitis. The aim of this retrospective pilot study was to evaluate the RTL of first maxillary premolar and to study the correlation with age and sex in this value. METHODS:The sample included 110 cone beam computed tomography (CBCT) images...

Background: Aim of this study was to compare coronally advanced flap (CAF) and composite restoration of the cement-enamel junction (CEJ) with or without connective tissue graft (CTG) for treatment of single maxillary gingival recession with non-carious cervical lesion (NCCL). Material and methods: Thirty patients with single gingival recessions...

Background: Individuals with altered passive eruption (APE) are assumed to be more susceptible to periodontal diseases. To date, this hypothesis has not been sufficiently supported by scientific evidence. The aim of this study, using an experimental gingivitis model, was to examine the development and resolution of gingival inflammation in patient...

An interesting case report showing the benefits of the Cortical Lamina Technique in horizontal ridge augmentation

The early wound healing score (EHS) was introduced to assess early wound healing of periodontal soft tissues after surgical incision. The purpose of this study is to evaluate the intra-and inter-examiner reliability of the EHS. Six examiners with di↵erent levels of training and clinical focus were enrolled. Each examiner was trained on the use of t...

Purpose of review: To explore the pathogenic association between periodontal disease and rheumatoid arthritis focusing on the role of Porphyromonas gingivalis. Recent findings: In the last decades our knowledge about the pathogenesis of rheumatoid arthritis substantially changed. Several evidences demonstrated that the initial production of auto...

Background: Proper wound healing after regenerative surgical procedures is an essential issue for clinical success. Guided tissue regeneration (GTR) and application of enamel matrix derivatives (EMD) are common means to regenerate periodontal tissues. Both methods bear considerable advantages due to their special characteristics, but also go along...

The strict link between periodontitis (PD) and rheumatoid arthritis (RA) has been widely demonstrated by several studies. PD is significantly more frequent in RA patients in comparison with healthy subjects: this prevalence is higher in individuals at the earliest stages of disease and in seropositive patients. This is probably related to the role...

Objectives: The aim of this randomized controlled clinical trial was to evaluate the possible advantages of adjunctive hyaluronic acid (HA) application in the coronally advanced flap (CAF) procedure in single Miller class I/recession type 1 (RT1) gingival recession treatment. Material and methods: Thirty patients with one recession were enrolled...

Introduction A cemental tear (CeT) is a special type of surface root fracture that may cause periodontal and even periapical tissue destruction. Unfortunately, there is limited knowledge as to how these rare cases can effectively be treated. The present case is believed to be the first reported in the literature treating a bony defect caused by a c...

Background Different host proteins play a central role in cell response during bacterial infections, the Bcl-2-Associated X protein (BAX) and Vascular Cell Adhesion Protein 1 (VCAM-1) are often reported in infective primary events during cell injury. Objective The aim of this study is to evaluate the predictive value of these two proteins as bioma...

The figure 2 of the original version of this article was incorrect. Correct figure is presented below.

Purpose Numerous indices have been proposed to analyse wound healing in oral soft tissues, but each has specific shortcomings. A new method of analysis, the Early Wound Healing Score (EHS), was evaluated in the present study. The aim was to assess more accurately early healing by primary intention of surgical incisions in periodontal soft tissues....

Various classification systems have been proposed to describe furcation lesions and Glickman’s classification for many years seems to have been the most widely utilized in the sole clinical diagnosis with no reference to the prognostic value of the lesion itself. This article reviews the previous classification systems and proposes a new method to...

Objective Several evidences suggested a link between human microbiome and Rheumatoid Arthritis (RA) development. Porphyromonas gingivalis (P. gingivalis) seems involved in RA initiation and progression, as supported by the high occurrence of periodontitis. In this case‐control study, we analyzed tongue P. gingivalis presence and quantification in a...

Background: To calculate the prevalence of dehiscences and fenestrations and measure the buccal alveolar bone width overlying healthy mandibular incisors and canines. Methods: Cone Beam Computerized Tomographies (CBCTs) from patients aged 18 to 30 years were selected from a private database. The thickness of buccal bone in the sagittal scan was...

Objective: Metabolic syndrome (MetS) is defined as a spectrum of conditions associated with an increased risk of developing CVD and type 2 diabetes. MetS include: hyperglycemia, hypertension, visceral obesity, dyslipidemia with elevated values of triglycerides (TG) and low levels of HDL. The aim of this review is to provide current knowledge of th...

AimThe aim of the present study was to evaluate the effect on peri-implant mucosal inflammation from the use of a novel instrument made of chitosan in the non-surgical treatment of mild peri-implantitis across several clinical centers. Materials and methodsIn this 6-month multicenter prospective consecutive case series performed in six different pe...

BACKGROUND: Experimental pen-implant mucositis has been studied from various prospective in a duration of 21 days. Given the higher sensitivity of pen-implant mucosa the aim of the present study was to evaluate if a duration of 14 days would be sufficient to establish a state of measurable inflammation. METHODS: Twenty patients of age 57 11-year-ol...

Background: This randomized clinical trial evaluated the clinical and microbiological effects of 0.147% ethyl lauroyl arginate (LAE)-containing mouthwash compared to 0.12% chlorhexidine (CHX) mouthwash in the treatment of periodontitis. Materials and methods: Subjects were randomized to use 0.147% LAE and 0.12% CHX mouthwash after professional m...

Caratteristiche del campione • A questo studio hanno aderito 26 professionisti e 149 pazienti intervistati dopo la proposta terapeutica di sostituzione di un elemento naturale con impianto dentale. • I professionisti inclusi mostravano una trentennale esperienza professionale (29 anni in media). Nel 50% dei casi erano soci SIdP (20% soci attivi). •...

Background The aim of this study was to evaluate the in vivo incidence and the location of fenestrations in a young Italian population by using CBCT. Materials and Methods Fifty patients who had previously performed CBCT for planning third molar extraction or orthodontic therapy were selected for the study. No previous dental treatment had been pe...

Background: Experimental peri-implant mucositis has been studied from various prospective in a duration of 21 days. Given the higher sensitivity of peri-implant mucosa the aim of the present study was to evaluate if a duration of 14 days would be sufficient to establish a state of measurable inflammation. Materials and methods: 20 patients of ag...

Background: Peri-implantitis is a frequent disease that may lead to implant loss. The aim of this case series was to evaluate the clinical results of a new non-surgical treatment protocol. Methods: Fifteen patients with dental implants affected by peri-implantitis were treated with a multiple anti-infective non-surgical treatment (MAINST) which...

The cover image, by Enrica Vescarelli et al., is based on the In Vitro Research Autophagy activation is required for myofibroblast differentiation during healing of oral mucosa, DOI: 10.1111/jcpe.12767.

Aim: To comparatively evaluate the outcomes of professionally administered plaque removal (PAPR) and photodynamic therapy (PDT) in the treatment for experimentally induced gingivitis (G) and peri-implant mucositis (p-iM). Materials and methods: Twenty systemically healthy patients, each contributing a single implant-supported unit in a posterior...

Aim: It is known that periodontal tissues heal faster that skin, and gingiva in particular heal without scar formation. The mechanisms regulating this behavior are still unclear. The aim of our work is to compare wound healing in oral mucosa and gingiva, investigating the role of αSMA-expressing myofibroblasts and autophagy. Materials and methods...

Background: This retrospective study aimed to assess the compliance to supportive periodontal therapy (SPT) of implant treated patients with different periodontitis history and the possible influence of their compliance on peri-implant marginal bone level. Material and methods: Dental records of 106 patients treated with at least one dental impl...

Background P. gingivalis is a Gram-negative anaerobic bacterium usually located in the oral cavity, as component of microbiome. Next to the established association with oral cavity diseases, such as periodontitis and halitosis, in the last years a growing interest has been addressed to its implication in the development of autoimmune diseases, such...

Background: The aim of the study is evaluate the effectiveness of a chitosan brush on the treatment of mild peri-implantitis. Methods: Fifteen patients diagnosed with mild peri-implantitis contributing with single implant were treated with a chitosan brush. Modified plaque index (mPll), modified bleeding index (mBoP) and probing depth (PPD) were...

Aim: to evaluate the adjunctive clinical efficacy of probiotics in the treatment of peri-implant mucositis (p-iM) with professionally administered plaque removal (PAPR) and photodynamic therapy (PDT). Materials and methods: Following p-iM induction, patients underwent PAPR+PDT and were randomly assigned to receive the professional and home-based...

Objectives: The purpose of this study was to evaluate the effects of three distinct periodontal treatment methods in comparison with hand instrumentation on residual cementum of periodontal diseased teeth. Cementum can influence the activities of periodontal cells and may play an important regulatory role in periodontal treatment. The ideal method...

Background: Aim of this study was to assess the clinical efficacy of coronally advanced flap (CAF) with or without connective tissue graft (CTG) for the treatment of Multiple Adjacent Gingival Recessions (MAGR) in the upper arch. Material and methods: Thirty-two patients with a total of 74 gingival recessions were randomly allocated to the two g...

Objective: The aim of this study was to test specific stem cells that could enhance bone formation in combination with specific scaffolds. Methods: Dental Pulp Stem Cells (DPSCs) were seeded with Granular Deproteinized Bovine Bone (GDPB) or Beta-Tricalcium Phosphate (ß-TCP) in a rat model of calvarial "critical size" defect. DPSCs were isolated fro...

There is a paucity of data for the effectiveness of reconstructive procedures in the treatment of peri-implantitis. The objective of this study was to compare reconstruction of peri-implant osseous defects with open flap debridement (OFD) plus porous titanium granules (PTGs) compared with OFD alone. Sixty-three patients (36 female, 27 male; mean ag...

Periodontal disease is often responsible for the loss of attachment around teeth and therefore a major cause of ridge deficiency after the teeth are extracted. The world has become more and more aware of aesthetics in dentistry and any procedure aimed to preserve the hard and soft tissue becomes useful to satisfy the needs of increasingly demanding...

The aesthetics of the smile is related mainly to the amount of gingiva and tooth exposed when smiling, the shape, colour and symmetry of the teeth and the surrounding soft tissue should create a harmonic ‘ensemble’. The appearance of ‘short teeth’ may be due to an excessive gingival display. Excessive display of the pink aesthetics while smiling is...

Altered passive eruption is a clinical condition extensively described by the periodontal literature that results in a gingival display that is different from normal. Patients can also display severe inflammation when their plaque control is not adequate. This paper will explain how to diagnose and treat cases of altered passive eruption in a simpl...

Introduction: The aim of this retrospective study was to compare the clinical efficacy of four different surgical techniques in promoting periodontal regeneration in patients with infrabony defects: open flap debridement, application of enamel matrix derivatives (EMD), nanohydroxyapatite (nanoHA) application, and combined nanoHA and EMD applicatio...

A non-smoker male patient of 40 years old, sistemically and periodontally healthy, presented with an implant inserted 3 years ago showing bone loss and bleeding on probing. An open flap approach with removal of granulation tissue and debridment implant surface has been performed with a titanium rotating brush (Peribrush, Tigran, Sweden). In the SPT...

Altered passive eruption is described as a condition in which the relationship between teeth, alveolar bone, and the soft tissues creates an excessive gingival display and, in turn, in some circumstances, it may reveal a clinical aspect also known as the "gummy smile. " The surgical management of such cases is well understood and has been widely de...

Bone regeneration strategies in dentistry utilize biodegradable scaffolds seeded with stem cells able to induce bone formation. However, data on regeneration capacity of these tissue engineering constructs are still deficient. In this study micro-Computed tomography (micro-CT) and positron emission tomography (PET) analyses were used to investigate...

Nano-hydroxyapatite (nHA) is a potential ideal biomaterial for bone regeneration. However, studies have yet to characterize the behavior of human osteoblasts derived from alveolar bone on nHA. Thus, the aim of the present study was to evaluate the influence of nHA on the adhesion, proliferation and differentiation of these alveolar bone-derived cel...

One of the most important factors implicated in preterm birth (PTB) is acute genitourinary tract infection. The bacteria causing chronic periodontal inflammation include Gram-negative rods and anaerobes similar to those found in women with bacterial vaginosis. The aim of this prospective study is to investigate the relationship between oral and vag...

Background: The treatment of gingival recessions is one of the important branches of periodontics related to esthetics. However, no surgical technique has been reported for the treatment of a particular type of gingival recession called Stillman's cleft. Methods: The technique proposed is a laterally moved, coronally advanced flap. After local a...

The aim of this work was to present data from a large sample of patients with Temporo-Mandibular Disorders (TMD) in order to clarify some aspects of the development of pathological conditions that affect large parts of the population. This preliminary work put in relation, through an epidemiological evaluation, anamnestic and clinical data collecte...

Bone regeneration and bone fixation strategies in dentistry utilize scaffolds containing regenerating-competent cells as a replacement of the missing bone portions and gradually replaced by autologous tissues. Mesenchymal stem cells represent an ideal cell population for scaffold-based tissue engineering. Among them, dental pulp stem cells (DPSCs)...

Objective: Chlorhexidine (CHX) is considered the gold standard against gram-negative microorganisms. Little has been written about the simultaneous influence that both time and concentration could have on antiplaque formation effectiveness of CHX. The aim of this study is to compare the clinical and microbiological effectiveness of two different C...