André Pina

André Pina
University of Porto | UP · BioSIM (UCIBIO@REQUIMTE) / Faculdade de Medicina

MSc Biochemistry

About

8
Publications
481
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48
Citations
Citations since 2016
8 Research Items
48 Citations
201620172018201920202021202205101520
201620172018201920202021202205101520
201620172018201920202021202205101520
201620172018201920202021202205101520
Introduction
André F. Pina is currently a PhD student at the Faculty of Medicine of the University of Porto. His PhD project involves using molecular dynamics simulations and QM/MM methods to study the intricacies of the catalytic mechanism of Pyridoxal 5'-phosphate (PLP) synthase, a critical enzymatic complex of the PLP biosynthetic pathway, and a potential target against tuberculosis and malaria.
Additional affiliations
October 2018 - present
BioSIM Research Group
Position
  • PhD Student
Education
September 2018 - September 2022
University of Porto
Field of study
September 2015 - November 2017
University of Porto
Field of study
September 2012 - September 2015
University of Porto
Field of study

Publications

Publications (8)
Article
Enzymes catalyze a plethora of chemical reactions in nature. These biocatalysts offer a variety of benefits that no other synthetic catalyst can offer: high turnover, regio- and stereoselectivity, and ability to work under mild conditions and to react in environmentally friendly manufacturing processes. However, enzymes are not normally optimally s...
Article
The catalytic mechanism of Pdx2 was studied with atomic detail employing the computational ONIOM hybrid QM/MM methodology. Pdx2 employs a Cys-His-Glu catalytic triad to deaminate glutamine to glutamate and ammonia – the source of the nitrogen of pyridoxal 5’-phosphate (PLP). This enzyme is, therefore, a rate-limiting step in the PLP biosynthetic pa...
Article
Increased levels of cathepsin B (CatB), a cysteine protease, have been associated with different types of tumors, including prostate cancer. Hence, the identification of novel targeting ligands homing CatB may be a promising approach for the development of CatB-targeted therapies. In this work, a methodology called systematic evolution of ligands b...
Article
Full-text available
Background Non-B DNA conformations are molecular structures that do not follow the canonical DNA double helix. Mutagenetic instability in nuclear and mitochondrial DNA (mtDNA) genomes has been associated with simple non-B DNA conformations, as hairpins or more complex structures, as G-quadruplexes. One of these structures is Structure A, a cloverle...
Cover Page
Full-text available
An atomistic description of Pdx2 catalytic mechanism is provided by QM/MM methodologies. Pdx2, the glutaminase subunit of pyridoxal 5’-phosphate (PLP) synthase, is characterized by a Cys-His-Glu catalytic triad used to deaminate glutamine to glutamate. While being absent in humans, Pdx2 is essential for the development and replication of the protoz...
Poster
Full-text available
Non-B DNA conformations are molecules that do not follow the canonical double helix DNA structure[1]. This type of molecules, which can assume both simple (e.g. hairpins) and complex (e.g. G-quadruplexes) conformations, are highly related with mutagenetic instability in both nuclear and mitochondrial DNA (mtDNA)[2,3]. We have previously detected a...

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Projects

Projects (2)
Project
Our main question is to ascertain what types of non-B DNA conformations are related with mtDNA replication errors.
Project
Understanding the models behind the non-B DNA structures formation in human brain deletion rearrangement breakpoints