Andre Holzer

Andre Holzer
University of Cambridge | Cam · Christ's College

PhD in Bioinformatics; Master of Science in Molecular Biotechnology
Postdoctoral Research Associate at the University of Cambridge working on Microbial Systems Biology

About

22
Publications
5,687
Reads
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585
Citations
Additional affiliations
October 2017 - present
University of Cambridge
Position
  • Researcher
April 2015 - October 2015
Heidelberg University
Position
  • Tutor in Physical Chemistry
September 2014 - March 2015
University of Cambridge
Position
  • Visiting Student
Education
October 2017 - September 2021
September 2013 - October 2017
Heidelberg University
Field of study
September 2010 - September 2013
Heidelberg University
Field of study

Publications

Publications (22)
Article
Full-text available
Eukaryotic microalgae and prokaryotic cyanobacteria are the major components of the phytoplankton. Determining factors that govern growth of these primary producers, and how they interact, is therefore essential to understanding aquatic ecosystem productivity. Over half of microalgal species representing marine and freshwater habitats require for g...
Article
Full-text available
The corrinoid B12 is synthesised only by prokaryotes yet is widely required by eukaryotes as an enzyme cofactor. Microalgae have evolved B12 dependence on multiple occasions and we previously demonstrated that experimental evolution of the non-B12-requiring alga Chlamydomonas reinhardtii in media supplemented with B12 generated a B12-dependent muta...
Article
Full-text available
Micro-organisms are ubiquitous and form complex communities whenever they inhabit the same environment. Well-known examples of microbial communities include the human gut microbiome, but equally important to life on the planet are those in the aquatic environment. These are made up of photosynthetic algae and bacteria as well as many other protists...
Article
Full-text available
Clean freshwater is fundamental to societies. However, globally, one in three people do not have access to it − a situation which demands more comprehensive and affordable monitoring frameworks. Gates Cambridge Scholars Andre Holzer and Maximilian Stammnitz have built PuntSeq, a citizen-science project, to address this challenge.
Article
Full-text available
While traditional microbiological freshwater tests focus on the detection of specific bacterial indicator species, including pathogens, direct tracing of all aquatic DNA through metagenomics poses a profound alternative. Yet, in situ metagenomic water surveys face substantial challenges in cost and logistics. Here, we present a simple, fast, cost-e...
Article
The isolation of sufficient amounts of intact nuclei is essential to obtain high-resolution maps of chromatin accessibility via assay for transposase-accessible chromatin using sequencing (ATAC-seq). Here, we present a protocol for tag-free isolation of nuclei from both cell walled and cell wall-deficient strains of the green model alga Chlamydomon...
Article
Full-text available
Microalgae play an essential role in global net primary productivity and global biogeochemical cycling. Despite their phototrophic lifestyle, over half of algal species depend for growth on acquiring an external supply of the corrinoid vitamin B12 (cobalamin), a micronutrient produced only by a subset of prokaryotic organisms. Previous studies have...
Preprint
Full-text available
Microalgae play an essential role in global net primary productivity and global biogeochemical cycling, but despite their phototrophic lifestyle, over half of algal species depend on a supply of the corrinoid vitamin B 12 (cobalamin) for growth. This essential organic micronutrient is produced only by a subset of prokaryotic organisms, which implie...
Article
Full-text available
Vitamin B 12 is an essential nutrient that is not made by plants; consequently, unfortified plant-based foods are not a reliable supply. Recent estimates suggest high rates of vitamin B 12 deficiency among the vegetarian and vegan populations, particularly in pregnant women or women of child-bearing age who, for ethical and health reasons, are shif...
Article
Full-text available
Thiamine pyrophosphate (TPP), an essential co‐factor for all species, is biosynthesised through a metabolically expensive pathway regulated by TPP riboswitches in bacteria, fungi, plants and green algae. Diatoms are microalgae responsible for c. 20% of global primary production. They have been predicted to contain TPP aptamers in the 3′UTR of some...
Preprint
Full-text available
Thiamine pyrophosphate (TPP), an essential co-factor for all species, is biosynthesised through a metabolically expensive pathway regulated by TPP riboswitches in bacteria, fungi, plants and green algae. Diatoms are microalgae responsible for approximately 20% of global primary production. They have been predicted to contain TPP aptamers in the 3'U...
Thesis
Algae, a globally widespread group of photosynthetic eukaryotes, are the primary producers in the aquatic world. Their photoautotrophic lifestyle together with rapid growth rates and diverse metabolic capabilities make them sustainable, renewable and environmentally friendly production platforms with great potential for biotechnological application...
Article
Full-text available
Chlamydomonas reinhardtii has many attractive features for use as a model organism for both fundamental studies and as a biotechnological platform. Nonetheless, despite the many molecular tools and resources that have been developed, there are challenges for its successful engineering, in particular to obtain reproducible and high levels of transge...
Preprint
Full-text available
Chlamydomonas reinhardtii has many attractive features for use as a model organism for both fundamental studies and as a biotechnological platform. Nonetheless, despite the many molecular tools and resources that have been developed, there are challenges for its successful engineering, in particular to obtain reproducible and high levels of transge...
Preprint
Full-text available
Clean freshwater lies at the heart of human society and monitoring its quality is paramount. In addition to chemical controls, traditional microbiological water tests focus on the detection of specific bacterial pathogens. The direct tracing of all aquatic DNA poses a more profound alternative. Yet, this has hitherto been underused due to challenge...
Preprint
Full-text available
The corrinoid B12 is synthesised only by prokaryotes yet is widely required by eukaryotes as an enzyme cofactor. Microalgae have evolved B12 dependence on multiple occasions and we previously demonstrated that experimental evolution of the non-requiring alga Chlamydomonas reinhardtii in media supplemented with B12 generated a B12-dependent mutant (...
Data
A repository for assembled reference genomes of the green model alga Chlamydomonas reinhardtii including files optimised for visualisation in IGV (Integrative Genomics Viewer, http://software.broadinstitute.org/software/igv/). Based on data from Merchant et al. (2007) The Chlamydomonas genome reveals the evolution of key animal and plant function...
Poster
Full-text available
Year by year, Cambridge rowers, swimmers and punters claim to experience serious infections associated with pathogens obtained from our river Cam’s water. To get a better qualitative understanding of this issue, we have conducted a public online survey assessing Cambridge citizens’ general contact with the river as well as people’s actual experienc...
Thesis
The expression of human genes is controlled by cis-regulatory elements, so-called enhancers, which influence activation of a given gene from a genomic distance of up to one megabase. The activity of individual enhancers is regulated by the chromatin landscape and allows cell-type specific gene regulation by interacting with a specific set of promot...
Article
The extended second order algebraic-diagrammatic construction (ADC(2)-x) scheme for the polarization operator in combination with core-valence separation (CVS) approximation is well known to be a powerful quantum chemical method for the calculation of core-excited states and the description of X-ray absorption spectra. For the first time, the imple...

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