Andre Gustavo Machado Bertran

Andre Gustavo Machado Bertran
Wageningen University & Research | WUR · Department of Nematology

PhD
Post-doc at the laboratory of Nematology of Wageningen University

About

12
Publications
1,265
Reads
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51
Citations
Citations since 2017
6 Research Items
35 Citations
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201720182019202020212022202302468101214
201720182019202020212022202302468101214
201720182019202020212022202302468101214
Introduction
I am a molecular biologist with experience in plant virology and nematology. I currently work on a project aimed at unravelling the chemical communication between potato cyst nematodes and potato plants funded by the Holland Innovative Potato consortium.
Additional affiliations
March 2010 - April 2016
University of Brasília
Position
  • Doctorate Student
Education
March 2012 - April 2016
University of Brasília (UnB)
Field of study
  • Molecular Biology (Virology)
March 2010 - February 2012
University of Brasília (UnB)
Field of study
  • Molecular Biology (Virology)

Publications

Publications (12)
Article
Full-text available
Cyst nematodes are considered a dominant threat to yield for a wide range of major food crops. Current control strategies are mainly dependent on crop rotation and the use of resistant cultivars. Various crops exhibit single dominant resistance (R) genes that are able to activate effective host-specific resistance to certain cyst nematode species a...
Article
Full-text available
The activity of intracellular plant Nucleotide-Binding Leucine-Rich Repeat (NB-LRR) immune receptors is fine-tuned by interactions between the receptors and their partners. Identifying NB-LRR interacting proteins is therefore crucial to advance our understanding of how these receptors function. A co-immunoprecipitation/mass spectrometry screening w...
Article
Full-text available
To identify host factors for tomato spotted wilt orthotospovirus (TSWV), a virus-induced gene silencing (VIGS) screen using tobacco rattle virus (TRV) was performed on Nicotiana benthamiana for TSWV susceptibility. To rule out any negative effect on the plants' performance due to a double viral infection, the method was optimized to allow screening...
Article
Full-text available
The tripartite genome of the negative-stranded RNA virus Tomato spotted wilt orthotospovirus (TSWV) is assembled, together with two viral proteins, the nucleocapsid protein and the RNA-dependent RNA polymerase, into infectious ribonucleoprotein complexes (RNPs). These two viral proteins are, together, essential for viral replication and transcripti...
Article
Full-text available
Nematodes are presumably the most abundant Metazoa on Earth, and can even be found in some of the most hostile environments of our planet. Various types of hypobiosis evolved to adapt their life cycles to such harsh environmental conditions. The five most distal major clades of the phylum Nematoda (Clades 8–12), formerly referred to as the Secernen...
Article
Full-text available
Frequencies of CpG and UpA dinucleotides in most plant RNA virus genomes show degrees of suppression comparable to those of vertebrate RNA viruses. While pathways that target CpG and UpAs in HIV-1 and echovirus 7 genomes and restrict their replication have been partly characterised, whether an analogous process drives dinucleotide underrepresentati...
Article
Polygonum ringspot virus (PolRSV) is a recently characterized tospovirus reported in Italy. Northern blot analyses of PolRSV infections in Nicotiana benthamiana (NB) and tomato plants showed that a viral RNA species with nearly twice the length of the genomic S-RNA accumulated abundantly in the former host, but was not detected in the latter. Addit...
Article
Full-text available
The tospoviral RNA-dependent RNA polymerases (RdRp), or L proteins, perform several conserved functions during virus replication in host cells. In this study, an L segment sequence of 9,040 bp from a new tospovirus (family Bunyaviridae) naturally infecting bean (Phaseolus vulgaris L.) plants was characterized. It encodes the largest RdRp gene known...
Article
Full-text available
Groundnut ringspot virus is a negative-sense single-stranded RNA virus that belongs to the genus Tospovirus and is the prevalent member of this genus in Brazil. This work presents the nucleotide sequence of the L RNA, with a single open reading frame of 2873 amino acids in the complementary strand corresponding to the RNA-dependent RNA polymerase (...

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Projects

Projects (2)
Project
Different strategies for the establishment of a reverse genetics system for TSWV based on mini-replicon activity and infectious clone generation