• Home
  • AZTI
  • Marine Research Division
  • Anders Lanzén
Anders Lanzén

Anders Lanzén
AZTI · Marine Research Division

PhD
Looking for collaborators for coordinated or meta-study of estuary sediment metabarcoding

About

265
Publications
32,623
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
7,110
Citations
Introduction
Anders Lanzén currently works at the Marine Research Division, AZTI. Anders does research in microbial ecology, genomics based environmental monitoring and computational biology.
Additional affiliations
January 2018 - present
AZTI
Position
  • Research Associate
Description
  • Four year Ikerbasque research fellowship focussed on monitoring of ecological impact assessment of anthropogenic impacts in the marine environment using environmental genomics
November 2017 - December 2017
Norce Research
Position
  • Research Associate
May 2013 - November 2017
Neiker-Tecnalia Basque Institute for Agricultural Research and Development
Position
  • PostDoc Position
Description
  • Two-year Marie Curie postdoc focussed on using high-throughput sequencing of the soil microbiome for predicting and monitoring of the effects of climate change in alpine ecosystems
Education
August 1996 - September 2002
Linköping University
Field of study
  • Engineering biology

Publications

Publications (265)
Article
Full-text available
Sequencing of taxonomic or phylogenetic markers is becoming a fast and efficient method for studying environmental microbial communities. This has resulted in a steadily growing collection of marker sequences, most notably of the small-subunit (SSU) ribosomal RNA gene, and an increased understanding of microbial phylogeny, diversity and community c...
Article
Genomics is fast becoming a routine tool in medical diagnostics and cutting-edge biotechnologies. Yet, its use for environmental biomonitoring is still considered a futuristic ideal. Until now, environmental genomics was mainly used as a replacement of the burdensome morphological identification, to screen known morphologically distinguishable bioi...
Article
Full-text available
A decade after environmental scientists integrated high‐throughput sequencing technologies in their toolbox, the genomics‐based monitoring of anthropogenic impacts on the biodiversity and functioning of ecosystems is yet to be implemented by regulatory frameworks. Despite the broadly acknowledged potential of environmental genomics to this end, tec...
Article
Routine monitoring of benthic biodiversity is critical for managing and understanding the anthropogenic impacts on marine, transitional and freshwater ecosystems. However, traditional reliance on morphological identification generally makes it cost‐prohibitive to increase the ambition of monitoring programs. Metabarcoding of environmental DNA has a...
Article
Full-text available
Apart from its contribution to climate change, offshore oil and gas extraction is also a potential threat to the diversity and function of marine ecosystems. Routine monitoring of the environmental status of affected areas is therefore critical for effective management. While current morphology-based monitoring is relatively time consuming, costly...
Article
To avoid loss of genetic information in environmental DNA (eDNA) field samples, the preservation of nucleic acids during field sampling is a critical step. In the development of standard operating procedures (SOPs) for eDNA-based compliance monitoring, the effect of different routinely used sediment preservations on biological community structures...
Article
Full-text available
Environmental DNA (eDNA) metabarcoding (parallel sequencing of DNA/RNA for identification of whole communities within a targeted group) is revolutionizing the field of aquatic biomonitoring. To date, most metabarcoding studies aiming to assess the ecological status of aquatic ecosystems have focused on water eDNA and macroinvertebrate bulk samples....
Article
Crop harvest followed by a fallow period can act as a disturbance on soil microbial communities. Cropping systems intended to improve alpha-diversity of communities may also confer increased compositional stability during succeeding growing seasons. Over a single growing season in a long-term (18 year) agricultural field experiment incorporating co...
Article
Microbial communities inhabiting soil-water-sediment continuum in coastal areas provide important ecosystem services. Their adaptation in response to environmental stressors, particularly mitigating the impact of pollutants discharged from human activities, has been considered for the development of microbial biomonitoring tools, but their use is s...
Article
Full-text available
Environmental DNA methods such as metabarcoding have been suggested as possible alternatives or complements to the current practice of morphology-based diversity assessment for characterizing benthic communities in marine sediment. However, the source volume used in sediment eDNA studies is several magnitudes lower than that used in morphological i...
Article
Full-text available
Metabarcoding of environmental DNA (eDNA) is an attractive complement to morphological methods for surveys and routine monitoring of marine sediment benthic communities. However, metabarcoding and other genetic techniques are heavily affected by choices made during sampling, processing, and analysis. Here, we investigated the effect of different eD...
Presentation
Full-text available
comparsion between traditional monitoring, based on morphological taxonomy of marine macroinvertebrates, and molecular-based methods. The focus is in determining reference conditions to calculate M-AMBI based on metabarcoding, in Basque estuaries and coasts
Conference Paper
Full-text available
Routine biological monitoring of the areas affected by offshore oil drilling and extraction is critical for ensuring proper environmental management. In addition to sufficient knowledge of the ecosystem affected, formalised e.g. as biotic indices of indicator species, adequate temporal and spatial resolution is also required, to provide accurate in...
Article
Full-text available
Meromictic lakes are permanently stratified lakes that display steep gradients in salinity, oxygen and sulphur compounds tightly linked to bacterial community structure and diversity. Lake Sælenvannet is a meromictic lake located south of Bergen, Norway. The 26 m deep lake is connected to the open sea and permanently stratified into two layers sepa...
Article
Increased exploitation of resources in sensitive marine ecosystems emphasizes the importance of knowledge regarding ecological impacts. However, current bio-monitoring practices are limited in terms of target-organisms and temporal resolution. Hence, developing new technologies is vital for enhanced ecosystem understanding. In this study, we have a...
Preprint
Full-text available
Resistance is the capacity for a community to remain unchanged, and resilience the capacity to return to an original state, in response to disturbance. Increasing species richness may increase both dynamics. In a long-term agricultural field experiment incorporating conventional (CON), integrated (INT), conservation (CA) and organic (ORG) cropping...
Article
Full-text available
Motivation: Technological advances in metatranscriptomics have enabled a deeper understanding of the structure and function of microbial communities. "Total RNA" metatranscriptomics, sequencing of total reverse transcribed RNA, provides a unique opportunity to investigate both the structure and function of active microbial communities from all thr...
Article
Full-text available
Recycling of wood ash from energy production may counteract soil acidification and return essential nutrients to soils. However, wood ash amendment affects soil physicochemical parameters that control composition and functional expression of the soil microbial community. Here, we applied Total RNA-sequencing to simultaneously assess the impact of w...
Preprint
Full-text available
A decade after environmental scientists integrated high-throughput sequencing technologies in their toolbox, the genomics-based monitoring of anthropogenic impacts on biodiversity and ecosystems is yet to be implemented by regulatory frameworks. Despite the broadly acknowledged potential of environmental DNA and RNA to cost-efficiently and accurate...
Conference Paper
Full-text available
Despite being a promising technology for remediating metal(loid)-contaminated land, the success and effectiveness of phytotechnologies often face important challenges, e.g. selection of suited plant species, budgetary restrictions, an upcoming climate change scenario, or site-specific limitations for deploying best agronomic practices. An ultimate...
Article
Full-text available
Background Metatranscriptomics has been used widely for investigation and quantification of microbial communities’ activity in response to external stimuli. By assessing the genes expressed, metatranscriptomics provides an understanding of the interactions between different major functional guilds and the environment. Here, we present a de novo ass...
Preprint
Full-text available
Background: Metatranscriptomics has been used widely for investigation and quantification of microbial communities' activity in response to external stimuli. By assessing the genes expressed, metatranscriptomics provide an understanding of the interactions between different major functional guilds and the environment. Here, we present de-novo assem...
Code
Comparative Metatranscriptomics Workflow is a standardized and validated workflow to functionally classify quality filtered mRNA reads from metatranscriptomic or total RNA studies generated using NGS short reads. CoMW is used for classification of these reads using assembled contigs to the reference protein databases.
Preprint
Full-text available
Recycling of wood ash from energy production may counteract soil acidification and return essential nutrients to soils. However, wood ash amendment affects soil physicochemical parameters that control composition and functional expression of the soil microbial community. Here, we applied Total RNA-sequencing to simultaneously assess the impact of w...
Article
Technosols can be used to rehabilitate degraded land and reuse wastes. Ideally, these newly formed soils should also fulfil the main soil functions. In this study, initially, we characterized the physicochemical and microbial properties of different formulations and their ingredients (i.e., dirt from a waste recovery plant, recycled bentonite, sewa...
Article
Livestock manure-derived amendments can be beneficial for agricultural soil quality, as they can increase the content of soil organic matter and nutrients, stimulate microbial activity and biomass, and enhance crop yield. Here, we studied the impact of six different manure-derived amendments, according to the origin (horse manure-derived vs. chicke...
Conference Paper
Full-text available
The long-term recovery of soil ecological functions underlying ecosystem services should lie at the core of the assessment of any phytomanagement initiative. Soil microbial properties have demonstrated a high potential as indicators of the effectiveness of phytomanagement of metal(loid)-contaminated soils, as they are key players in many of these s...
Article
Full-text available
The microbial diversity associated with diffuse venting deep-sea hydrothermal deposits is tightly coupled to the geochemistry of the hydrothermal fluids. Previous 16S rRNA gene amplicon sequencing (metabarcoding) of marine iron-hydroxide deposits along the Arctic Mid Ocean Ridge, revealed the presence of diverse bacterial communities associated wit...
Article
Full-text available
In many urban catchments, the discharge of effluents from wastewater treatment plants (WWTPs), as well as untreated wastewaters (UWWs), presents a major challenge for the maintenance of river sediment and water quality. The discharge of these effluents cannot only increase the concentration of metals, nutrients and organic compounds in fluvial ecos...
Article
Metal resistance has been associated with antibiotic resistance due to co- or cross-resistance mechanisms. Here, metal contaminated mine soil treated with organic wastes was screened for the presence of mobile genetic elements (MGEs). The occurrence of conjugative IncP-1 and mobilizable IncQ plasmids, as well as of class 1 integrons, was confirmed...
Article
Full-text available
Mountain grasslands in the Iberian Peninsula are the result of extensive grazing. However, a progressive abandonment of grazing activity is currently observed in the study region. The objective of this work was to evaluate the short-term (2 years) effects of non-grazing on the diversity and composition of plants, soil microorganisms (prokaryotes, f...
Article
Full-text available
Human impact on marine benthic communities has traditionally been assessed using visible morphological traits and has focused on the macrobenthos, whereas the ecologically important organisms of the meio- and microbenthos have received less attention. DNA metabarcoding offers an alternative to this approach and enables a larger fraction of the biod...
Data
Taxonomic classification and category (protists, metazoa or unknown) for representative OTU sequences. (TSV)
Data
Rarefaction analysis of protist OTU richness for pooled samples (grey lines) and individual replicats (black lines). Mean and standard error of expected richness was calculated based on repeated random sub-samples at each read interval from replicates of the samples Fine Sand (A; n = 5), Coarse Sand (B; n = 7) and Clay (C, n = 5), after taxonomic f...
Data
Rarefaction analysis of metazoan OTU richness for pooled samples (grey lines) and individual replicates (black lines). Mean and standard error of expected richness was calculated based on repeated random sub-samples at each read interval from replicates of the samples Fine Sand (A; n = 5), Coarse Sand (B; n = 7) and Clay (C, n = 5), after taxonomic...
Data
Rarefaction analysis of pooled samples illustrating the effect of retaining singletons. Rarefaction curves using solid lines represent the studied dataset where singletons were retained (and correspond to Figs 1 and 2), whereas dashed lines result from rarefaction curves after removing singletons. (TIF)
Data
Overview of sequence depth, richness and taxonomic composition (protists and metazoa) for all replicates and pooled samples. (XLSX)
Article
Aided phytostabilization uses metal-tolerant plants, together with organic or inorganic amendments, to reduce metal bioavailability in soil while improving soil quality. The long-term effects of the following organic amendments were examined as part of an aided phytostabilization field study in an abandoned Pb/Zn mining area: cow slurry; sheep manu...
Article
Full-text available
As global exploitation of available resources increases, operations extend towards sensitive and previously protected ecosystems. It is important to monitor such areas in order to detect, understand and remediate environmental responses to stressors. The natural heterogeneity and complexity of communities means that accurate monitoring requires hig...
Article
Full-text available
Mountain elevation gradients are invaluable sites for understanding the effects of climate change on ecosystem function, community structure and distribution. However, relatively little is known about the impact on soil microbial communities, in spite of their importance for the functioning of the soil ecosystem. Previous studies of microbial diver...
Article
Full-text available
The effect of salinity on prokaryotic community diversity in Abijata-Shalla Soda Ash Concentration Pond system was investigated by using high-throughput 16S rRNA gene 454 pyrosequencing. Surface water and brine samples from five sites spanning a salinity range of 3.4 % (Lake Abijata) to 32 % (SP230F, crystallizer pond) were analyzed. Overall, 33 pr...
Article
Full-text available
Traditionally, conservation and management of mountain pastures has been managed solely on the basis of visible biota. However, microorganisms play a vital role for the functioning of the soil ecosystem and, hence, pasture sustainability. Here, we studied the links between soil microbial (belowground) community structure (using amplicon sequencing...
Data
Parameters and taxon abundances showing significant differences with land-use (P, pastures grazed year-round; X, winter grazing only; H, non-grazed, harvested). Width of notches indicates 95% confidence intervals of the median. p-values determined by group-wise ANOVA (and verified by Tukey's range test) are given below each boxplot.
Data
Parameters showing significant differences between sites with siliceous (S) and calcareous (C) bedrock. Distributions of values are illustrated as box plots. Only differences that could be verified independently in M or V sites alone are included. p-values determined by group-wise ANOVA (and verified by Tukey's range test) are given below each plot...
Data
Parameters showing differences between sampling years (2013 and 2014). Significantly different distributions of values measured for each year are illustrated as box plots. p-values determined by group-wise ANOVA (and verified by Tukey's range test) are given below each plot.
Data
Parameters showing significant differences between mountain (M) and valley (V) sites. Distributions of values measured at each elevation zone are illustrated as box plots. p-values determined by group-wise ANOVA (and verified by Tukey's range test) are given below each plot.
Data
Relative abundance as a function of pH for 10 taxa with inconsistent but significant correlation to pH in both M and V sites. Curves fitted using LOWESS (Locally Weighted Scatterplot Smoothing).
Data
Results from Analysis of Similarities (ANOSIM) between communities (Bray-Curtis), asterisks indicating significance strength.
Data
Samples with collection date, location, physical parameters measured and other metadata.
Data
Alpha diversity estimates for all samples.
Data
Best fitting solutions to the structural equation model (Figure 2), asterisks indicating significance strength.
Data
Parameters correlated to NMDS axes (significant correlations in bold).
Data
Taxa with relative abundance significantly correlated to parameters measured in mountain and valley sites.
Article
Full-text available
Svalbard, situated in the high Arctic, is an important past and present coal mining area. Dozens of abandoned waste rock piles can be found in the proximity of Longyearbyen. This environment offers a unique opportunity for studying the biological control over the weathering of sulphide rocks at low temperatures. Although the extension and impact of...