Ananda Ananda- Master of Science
- Lecturer at Andalas University
Ananda Ananda
- Master of Science
- Lecturer at Andalas University
About
20
Publications
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Introduction
Proud to be a scientist.
Skills and Expertise
Current institution
Additional affiliations
July 2018 - April 2020
July 2018 - April 2020
Publications
Publications (20)
This study investigates the motility and viability of Kokok Balenggek chicken (KBC) spermatozoa in various commercial physiological solutions, specifically Ringer's Lactate (RL), Sodium Chloride 0.9% (NaCl 0.9%), and Phosphate Buffered Saline (PBS), all stored at 4ºC. A total of 15 KBC specimens were utilized in this research. Semen collection occu...
This study investigated the impact of equilibration time on the quality of frozen-thawed semen in Pesisir bulls, focusing on post-thaw motility, viability, abnormalities, and intact plasma membrane. Semen samples were equilibrated for 2, 4, 6, 8, and 10 hours before freezing. Fresh semen had an average volume of 3.82mL, a sperm concentration of 762...
Background: The Kokok Balenggek rooster (KBR), with its distinct phenotypes—Biriang, Balang, Kinantan, and Kuriak—offers a unique opportunity to study variations in semen characteristics and protein profiles. Understanding these variations can aid in the development of better management strategies for poultry breeding programs. Aim: This study aime...
Pesisir bulls from West Sumatra, Indonesia, have the potential to be valuable meat producers due to their adaptability and unique characteristics, but reproductive challenges necessitate the use of artificial insemination (AI) technology. The success of AI relies on the quality of frozen semen, which is influenced by thawing conditions, making the...
Sperm quality plays a crucial role in determining the success of fertilization. This study aimed to assess the quality of both fresh and frozen ram semen, as well as examine changes in semen protein content. Four local rams, aged 3-4 years, were selected for the study. Semen collection was conducted in the morning using an artificial vagina. The co...
This study aims to determine the hatching performance of Kokok Balenggek chicken (KBC) using different males as the formation of superior local meat-type chickens. The study used an experimental method, randomized block design (RBD), with eight treatments and three groups. The treatment was male KBC (G0) and the group was the hatching period. Chick...
Kambing merupakan salah satu ternak ruminansia kecil sebagai penghasil daging dan susu (ternak dwiguna), tetapi susu yang banyak dijual dipasaran adalah susu sapi. Susu kambing tidak kalah nilai gizinya dibandingkan dengan susu sapi, selain memiliki keunggulan dalam kandungan proteinnya, susu kambing juga memiliki kandungan vitamin A dan vitamin B...
This study aims to know sperm quality and daily testosterone profile from fecal samples of the Kokok Balenggek rooster (KBR). This study used 10 KBR consisting of 1 Balang phenotype, 4 Biriang phenotypes, 1 Jalak phenotype, 2 Kinantan phenotypes, 1 Kuriak phenotype, and 1 Taduang phenotype. Semen was collected three times and evaluated for the qual...
This study assesses the impact of two sperm sexing techniques, the Bovine Serum Albumin (BSA) column method, and the swim-up (SU) method, on frozen buffalo spermatozoa quality. A total of 50 straws of frozen buffalo semen were used in this study. Spermatozoa quality was evaluated before (post thawing) and after the spermatozoa sexing process. Sperm...
Dalam eksplorasi ilmu tumbuhan, kita disuguhkan dengan
keberagaman dan keelokan flora yang memperindah bumi
kita. Tumbuhan, dengan kekayaan spesies dan sifat yang
unik, membuka tirai keajaiban alam selama ini. Tidak hanya
mengungkap kompleksitas dan pesona keindahan, interaksinya dengan
fauna di sekitar juga menggambarkan sebuah peta kehidupan yang...
Radiation techniques have gained significant attention in the field of agricultural wastewater management due to their effectiveness in treating diverse contaminants. This review aims to explore the effects and applications of radiation techniques, including ultraviolet (UV), gamma-ray, and electron beam (EB). UV radiation utilizes ultraviolet ligh...
The application of cryopreservation to preserve germplasm in such specific breed requires preliminary studies, primarily related to the resistance of spermatozoa to low temperatures (4 – 5 °C) as measured by their motility and longevity. In this study, semen taken from five phenotypes of Kokok Balengeek Chicken (KBC) (Biriang, Jalak, Kinantan, Kuri...
ABSTRAK Penelitian ini bertujuan untuk mendapatkan efektivitas herba ciplukan (Physalis angulata. L) sediaan ekstrak dan serbuk sebagai imunostimulan terhadap kekebalan broiler yang telah mendapatkan vaksinasi, dan diuji tantang dengan virus Newcastle disease. Materi yang digunakan adalah DOC sebanyak 96 ekor dengan bobot rata rata 35-40 g, herba c...
The research aimed to evaluate the effect of Zn supplementation in diet of Sikumbang Janti female duck on its performance, carcass, digestive organs, and intestinal morphology. The study used 96 female ducks aged 8 weeks. This research used a completely randomized design with four treatments (0, 30, 60, and 90 mg Zn/kg), each treatment was repeated...
This study aims to determine the effect of using the collagenase enzyme on the viability of chicken embryos' liver, kidney, and fibroblast cells in vitro. Liver and kidney organs were collected from embryonic chicken eggs at 15 and 19 days. Meanwhile, fibroblast cells were obtained using muscle tissue of 11-day-old chicken embryos. Each organ and t...
Tujuan penelitian ini untuk mengetahui efek pemberian kopi terhadap fungsi reproduksi dan gambaran histologi organ reproduksi mencit (Mus musculus) betina. Penelitian ini menggunakan metode eksperimental dengan dua kelompok perlakuan: kelompok kontrol dan kelompok pemberian kopi dengan dosis yang telah dikonversi setara dengan tiga cangkir kopi pad...
The aim of the present study was to determine the optimal incubation time of collagenase on concentration, viability, quality of endometrial cell culture, and the role of estradiol and progesterone on ovine endometrial cells proliferation. Endometrium minced was incubated into collagenase with different duration of time: 1 hour, 3 hours, and 6 hour...
Questions
Questions (10)
Good Evening everyone..
Let me introduce my self...
I have done it according to the protocol that is on some papers, but in negative control is also stained same as with positive control.
For primary antibody, I used CSFV Envelope Glycoprotein E2 Polyclonal Ab - Rabbit (Bioss)
For the secendory, I used Goat anti-Rabbit IgG (H+L) HRP Conjugate (Thermo)
and for staining I using AEC kit solution.
could u tell me the right protocol for it?
Please help me to solve this..
Thank you for your attention,
Best regards,
Ananda
Good evening everyone.
I'm currently working on the MDCC-MSB1 suspension cells. Do you have any protocol or references about freeze-thawing for it?
Thank you.
I have one question concerning antibody dilution. I got one vial containing 2 mg Goat Anti-Rabbit IgG FITC. If they say the working dilution is 1:100 in PBS, what does it mean that I take for exemple 1 μg of my stock plus 99 μL PBS?
Good morning everyone.
I'm currently working on the primary culture of chicken embryo liver cells and I want to use collagenase to get the cells. do you know the right isolation procedure?
thanks.
Good evening everyone..
Now I'm working with PRRS Virus that are adapted to MSB1 cell line,
Do you have any protocol or references about imunofluresence staining for it?
Thank you.
Good evening everyone.
do you have references related to the type of collagenase for cell culture, such as the optimum temperature, the duration of the optimum incubation time, and the mechanisms in breaking down the protein in detail?
Hello everyone..
Now, I work with ovine endometrial cell culture..
I will try to add progesterone to the culture medium.
I currently have progesterone that can only be dissolved in absolute ethanol, while absolute ethanol will damage the medium.
So, how is the right step to dissolve progesterone for ovine endometrial cell culture?
Thank you for your help,
Best regards,
Ananda
hello everyone..
Does anyone know the technique/protocol for immunofluoresence staining of endometrial cell culture in sheep/ovine?
Good evening everyone..
I want to isolation the epithelial and the stromal cells of the mice endometrium (Mus musculus)
and I'd like to know, how to get the cells?
could u tell me the protocol for the isolation of the cells?
maybe I get some cells, but it can't attach to the flask,
how we can attach the cells?
I have tried it, according to some techniques in some papers, but I can't get it..
Please help me to solve this..
Thank you for your attention,
