About
23
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Introduction
Anamika Rawat currently works as a Research scientist at the Desert Agriculture Initiative, KAUST. Anamika studies Plant microbe interactions, and stress biology in Vascular (Arabidopsis) and non vascular (Marchantia polymorpha) plants.
Current institution
Publications
Publications (23)
Background
Human-designed oligotrophic environments, such as cleanrooms, harbor unique microbial communities shaped by selective pressures like temperature, humidity, nutrient availability, cleaning reagents, and radiation. Maintaining the biological cleanliness of NASA’s mission-associated cleanrooms, where spacecraft are assembled and tested, is...
The complex and mutual interactions between plants and their associated microbiota are key for plant survival and fitness. From the myriad of microbes that exist in the soil, plants dynamically engineer their surrounding microbiome in response to varying environmental and nutrient conditions. The notion that the rhizosphere bacterial and fungal com...
The calcium‐dependent protein kinase CPK28 regulates several stress pathways in multiple plant species. Here, we aimed to discover CPK28‐associated proteins in Arabidopsis thaliana.
We used affinity‐based proteomics and identified several potential CPK28 binding partners, including the C7 Raf‐like kinases MRK1, RAF26, and RAF39. We used biochemistr...
Human-designed oligotrophic environments, such as cleanrooms, harbor unique microbial communities shaped by selective pressures like temperature, humidity, nutrient availability, cleaning reagents, and radiation. During the Phoenix spacecraft mission, genomes of 215 bacterial isolates were sequenced and based on overall genome related indices, 53 s...
Global climate change increasingly challenges agriculture with flooding and salinity. Among strategies to enhance crop resilience to these stresses, we tested several endophytic bacterial strains from mangroves, which are permanently exposed to flooding and high salinity. We show several strains that can enhance flooding and salinity tolerance in A...
The study of the whole of the genetic material contained within the microbial populations found in a certain environment is made possible by metagenomics. This technique enables a thorough knowledge of the variety, function, and interactions of microbial communities that are notoriously difficult to research. Due to the limitations of conventional...
Senescence is an important physiological process which directly affects many agronomic traits in plants. Senescence induces chlorophyll degradation, phytohormone changes, cellular structure damage, and altered gene regulation. Although these physiological outputs are well defined, the molecular mechanisms employed are not known. Using dark-induced...
The calcium-dependent protein kinase CPK28 is a regulator of immune homeostasis in multiple plant species. Here, we used a proteomics approach to uncover CPK28-associated proteins. We found that CPK28 associates with subfamily C7 Raf-like kinases MRK1, RAF26, and RAF39, and trans-phosphorylates RAF26 and RAF39. Metazoan Raf kinases function in mito...
The INDETERMINATE DOMAIN (IDD) family belongs to a group of plant-specific transcription factors that coordinates plant growth/development and immunity. However, the function and mode of action of IDDs during abiotic stress, such as salt, are poorly understood. We used idd4 transgenic lines and screened them under salt stress to find the involvemen...
The use of beneficial microbes to mitigate drought stress tolerance of plants is of great potential albeit little understood. We show here that a root endophytic desert bacterium, Pseudomonas argentinensis strain SA190, enhances drought stress tolerance in Arabidopsis. Transcriptome and genetic analysis demonstrate that SA190-induced root morphogen...
The use of beneficial microbes to mitigate drought stress tolerance of plants is of great potential albeit little understood. We show here that a root endophytic desert bacterium, Pseudomonas argentinensis sp. SA190, enhances drought stress tolerance in Arabidopsis. Transcriptome and genetic analysis demonstrate that SA190-induced root morphogenesi...
Expression of OXIDATIVE SIGNAL‐INDUCIBLE1 (OXI1) is induced by a number of stress conditions and regulates the interaction of plants with pathogenic and beneficial microbes.
In this work, we generated Arabidopsis OXI1 knockout and genomic OXI1 overexpression lines and show by transcriptome, proteome, and metabolome analysis that OXI1 triggers ALD1,...
Significance
Although plant growth–promoting bacteria (PGPB) enhance the performance of plants, only a few mechanisms have been identified so far. We show that the sulfur metabolisms in both PGPB Enterobacter sp. SA187 and Arabidopsis plants play a key role in plant salt stress tolerance. Salt stress induces a sulfur starvation response in plants t...
Although many endophytic plant growth‐promoting rhizobacteria have been identified, relatively little is still known about the mechanisms by which they enter plants and promote plant growth. The beneficial endophyte Enterobacter sp. SA187 was shown to maintain productivity of crops in extreme agricultural conditions. Here we present that roots of i...
Spatially directed cell division and expansion is important for plant growth and morphogenesis and relies on cooperation between the cytoskeleton and the secretory pathway. The phylogenetically conserved octameric complex exocyst mediates exocytotic vesicle tethering at the plasma membrane. Unlike other exocyst subunits of land plants, the core exo...
INDETERMINATE DOMAIN (IDD)/ BIRD proteins are a highly conserved plant-specific family of transcription factors which play multiple roles in plant development and physiology. Here, we show that mutation in IDD4/IMPERIAL EAGLE increases resistance to the hemi-biotrophic pathogen Pseudomonas syringae, indicating that IDD4 may act as a repressor of ba...
The exocyst, an evolutionarily conserved secretory vesicle‐tethering complex, spatially controls exocytosis and membrane turnover in fungi, metazoans and plants. The exocyst subunit EXO 70 exists in multiple paralogs in land plants, forming three conserved clades with assumed distinct roles. Here we report functional analysis of the first moss exoc...
Cortical microtubules (MTs) play a major role in the patterning of secondary cell wall (SCW) thickenings in tracheary elements (TEs) by determining the sites of SCW deposition. The EXO70A1 subunit of the exocyst secretory vesicle tethering complex was implicated to be important for TE development via the MT interaction. We investigated the subcellu...
Exocyst is an evolutionarily conserved vesicle tethering complex functioning especially in the last stage of exocytosis. Homologs of its eight canonical subunits – Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70, and Exo84 – were found also in higher plants and confirmed to form complexes in vivo, and to participate in cell growth including polarized e...
List of the 392 exocyst subunit sequences analyzed, including database accession numbers, phylogenetic classification and domain composition (Microsoft Excel file).
Protein sequences and alignments used for phylogenetic analyses (compressed Zip file containing protein sequences in text format – *.txt and alignment sequences in FASTA format – *.fst).
Extract of Bridelia retusa leaves was investigated as corrosion inhibitor of mild steel in 1 N H2SO4 using conventional weight loss, electrochemical polarizations, electrochemical impedance spectroscopy and scanning electron microscopic studies. The weight loss results showed that the extract of Bridelia retusa leaves is excellent corrosion inhibit...
We describe a DNA isolation procedure for chickpea (Cicer arietinum L.) which is rapid and less expensive without
involving ultra centrifugation or column purification steps. DNA preparation obtained from the present study was
essentially suitable for PCR analysis which is one of the key steps in crop improvement programme through marker
develop...
Questions
Questions (8)
Hello, I am measuring stomatal index in WT and mutant arabidopsis plant. The formula for SI says to divide the stomatal number by total number of stomata+epidermal cells. I am bit confused on how to count the epidermal cells, should I count only those that are entirely visible in my image or all the cells irrespective of their completeness? My mutant has bigger cell size, so if i count only the entire epidermal cells that are present in the image it is going to affect the stomatal index. Thanks.
Hello specialist in stomatas,
I would like to analyze the stomata at various stage of ABA and/or flg treatments. So I was wondering if its possible to fix the leaf samples and then analyze them later, so as to minimize the error.
Thanks.
Hello specialist in stomatas,
I would like to analyze the stomata at various stage of ABA and/or flg treatments. So I was wondering if its possible to fix the leaf samples and then analyze them later, so as to minimize the errors.
Thanks.
Hello, I would like to remove selection cassette from my mutant plant in order to transform another construct with same selection. I know, cre/lox recombination is one of the possibility, but couldn't find the detailed protocol for the same.
Can someone please share with me the protocol or tell me where to look for.
Thanks.
Hello, I would like to knock-in a tag at N-term. into my gene of interest. The strategy is to clone 1.promoter, 2. gene and 3. 3'UTR into the plasmid and then transform. Now the difficulty begins, I have a very very long gene with many small exons and introns, which is difficult to amplify. Are there any moss people here, who can will agree if I can clone cDNA instead of the whole gene and if in such case it would still be a Knockin?!
Thanks
Hi, is there Monopteros gene present in lower plants like marchantia or physcomitrella patens? Any references would be just perfect. Thanks
I am completely new in field of Marchantia! Would like to grow marchantia on Gamborg media. What is widely used for this purpose, confused between sigma and duchefa?
Needs some expert advice in purifying a 10,300 kb fragment (after restriction) from agarose gel? I am getting very low yield, only few ngs!!! whereas my starting concentrations is more than 5 ugs. Any suggestions!
