Ana Maria Testera

• University of Valladolid

Herein we present a novel one-pot method for the chemical modification of elastin-like recombinamers (ELRs) in a mild and efficient manner involving enzymatic catalysis with Candida antarctica lipase B. The introduction of different functionalities into such ELRs could open up new possibilities for the development of advanced biomaterials for regen...

Protein modification is an area of an intense research and many methods have been developed with applications in fields including biology, chemistry and medicine, as they are powerful tools for studying protein expression and localization, and engineering the functions of protein both in vitro and in live cells or in vivo to create new biocatalysts...

A bioactive and multifunctional elastin-like polymer (ELP) was produced by genetic engineering techniques to develop new artificial matrices with the ability to mimic the extracellular matrix (ECM). The basic composition of this ELP is a thermo- and pH-sensitive elastin pentapeptide which has been enriched with RGD-containing domains, the RGD loop...

Elastin-like recombinamer click gels (ELR-CGs) for biomedical applications, such as drug delivery or tissue engineering, have been developed by taking advantage of the click reaction (CuAAC) in the absence of traditional crosslinking agents. ELRs are functionalized with alkyne and azide groups using conventional chemical techniques to introduce the...

Herein we present a study of the effect of the addition of NaCl on the size and shape of thermally driven elastin-like block co-recombinamer (ELbcRs) self-assembled nano-objects. Many researchers are currently using NaCl to tune the transition temperature (T-t) of elastin-like recombinamers (ELRs). However, this method is used without a complete un...

ELR Catalyst-Free Click Gels (ELR-CFCGs) have been prepared and characterized by modifying both a structural ELR (VKV x 24) and a biofunctionalized ELR bearing RGD cell-adhesion sequences (HRGD6) to bear the reactive groups needed to form hydrogels via a click reaction. Prior to formation of the ELR-CFCGs, azide-bearing and cyclooctyne-modified ELR...

Layer-by-layer (LbL) is a mild and versatile surface modification technique that allows producing robust coatings even in substrates with complex geometries. Taking advantage of the 3D possibilities opened by LbL, we present customized compartmentalized capsules inspired by the complex structure of cells, with temperature and magnetic-based respons...

Recently, hydrogels have become popular in material research for biomedical applications, such as tissue engineering. In nature, tissues are formed by cells that are surrounded by the extracellular matrix (ECM). One of its components is the elastin. Such proteins can be used to create new polymers known as elastin-like recombinamers (ELRs), which c...

Nanostructured films consisting of polysaccharides and elastin-like recombinamers (ELRs) are fabricated in a layer-by-layer manner. A quartz-crystal microbalance with dissipation monitoring (QCM-D) is used to follow the build-up of hybrid films containing one polysaccharide (chitosan or alginate) and one of several ELRs that differ in terms of amin...

Thermoresponsive multilayer films consisting of ionic elastin-like recombinamers (ELRs) were prepared using a layer-by-layer technique on model substrates. The ELRpolymer containing lysine units served as a polycation and the one with glutamic acid served as a polyanion. The build up process was followed by UV/Vis absorption spectroscopy and atomic...

Here, we describe a procedure to manufacture smart hybrid probes that exhibit tunable optical properties as a function of multiple environmental variations. Initially, we achieved a one‐pot synthesis of gold‐PREP (photo‐responsive elastin‐like polymer) conjugate Gold‐AzoGlu15 via reduction of auric acid in the presence of PREP AzoGlu15 . Outstandin...

Chitosan and an elastin-like polymer, containing a specific osteoconductive sequence in the primary structure, have been combined to obtain bioactive injectable systems with enhanced mechanical properties and hydrogels. Obtained results indicate that the combination of such polymers may be very promising in the development of biomaterials for minim...

Costa et al. successfully apply a simple protein adsorption technique combining chitosan substrates and recombinant elastin-like polymers (ELPs) on page 3210. This process allows for coating of polysaccharide substrates with such biomimetic macromolecules, resulting in interesting properties such as smart behavior and improved cell adhesion. The fi...

Biotechnology offers powerful solutions to the challenges that arise during the design and development of new complex biomimetic materials to achieve specific biological responses. Recombinant DNA technologies, in particular, provide unique solutions in the biomaterials field, especially regarding the control of macromolecular architectures involvi...

Electrostatic self-assembly was used to fabricate new smart multi-layer coatings, using a recombinant elastin-like polymer (ELP) and chitosan as the counterion macromolecule. The ELP was bioproduced, purified and its purity and expected molecular weight were assessed. Aggregate size measurements, obtained by light scattering of dissolved ELP, were...

Elastin-like polymers (ELPs) are based on repeating sequences found in the natural elastin. They show remarkable combination of properties, such as a large range of mechanical properties, excellent biocompatibility, and smart behaviour and self-assembly towards temperature, pH and often different stimuli. Around a certain temperature, known as inve...

An elastin-like polymer (ELP) was designed by genetic engineering techniques to mimic the extracellular matrix (ECM). The precise control of the polymer sequence offered many advantages that allowed the definition of distinct and specific domains. The cell attachment domain present in the sequence enhances the cell adhesion. Hexamethylene diisocyan...

a comparative view on host physiology The organisers would like to thank Novozymes Delta Ltd who generously supported the meeting. Meeting

In spite of the enormous possibilities presented by macromolecules for the development of advanced materials with increased functionality, the achievement of functionality is often limited by the randomness associated with polymer synthesis and the exponential increase in technical difficulties encountered in attaining a desired degree of complexit...

The influence of modified α, β and γ-cyclodextrins (mCDs) on water solutions of the elastin-like thermo-responsive poly(VPGVG) has been studied in this work. The studied mCDs are hydroxypropyl-α-CD MS=0.6, hydroxypropyl-β-CD MS=0.6, hydroxypropyl-β-CD MS=0.8, hydroxypropyl-β-CD MS=1.0, hydroxypropyl-γ-CD MS=0.6, hydroxyethyl-β-CD MS=5.0 and methyl-...

The pH-responsive elastin-like polymers, [(PGVGV)(2)-(PGEGV)-(PGVGV)(2)](n) with n = 5, 9, 15, 30, 45, were obtained using genetic engineering and microbial protein expression. These were intended to study the effects of the molecular weight (MW) on the properties of their inverse temperature transition (ITT) and its dependence on pH. As a result,...

Genetic engineering techniques were used to design and biosynthesise an extracellular matrix (ECM) analogue. This was designed with a well-defined molecular architecture comprising different functional domains. The structural base is a elastin-derived repeating unit, which confers an adequate elastic characteristic. Some of these elastin domains ha...

The preparation of several enantiomerically pure β′-hydroxy-β-enaminoketones from the corresponding isoxazolic carbinols, which have been obtained by enzymatic kinetic resolution of the racemic β-hydroxyisoxazoles catalyzed by lipases, is described. The enzymatic transesterification of racemic (±)-5-(2-hydroxypropyl)-3-methylisoxazole 3a, and racem...