Ana Smolko

Ana Smolko
Ruđer Bošković Institute | RBI · Department of Molecular Biology

PhD

About

15
Publications
1,705
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103
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Introduction
Ana Smolko currently works in the Laboratory for Chemical Biology at the Department of Molecular Biology, Ruđer Bošković Institute. Ana does research in Molecular Biology, Plant Science and Biochemistry. Current project of the Laboratory for Chemical Biology is 'Phytohormones in abiotic stress of Brassica crops: mechanism of tolerance and application (PhytoBraCro).'

Publications

Publications (15)
Article
Full-text available
Salt and osmotic stress are the main abiotic stress factors affecting plant root growth and architecture. We investigated the effect of salt (100 mM NaCl) and osmotic (200 mM mannitol) stress on the auxin metabolome by UHPLC-MS/MS, auxin distribution by confocal microscopy, and transcript levels of selected genes by qRT-PCR in Arabidopsis thaliana...
Article
Full-text available
The control of plant growth and development is a well-coordinated process between exogenous and endogenous signals. Auxins are plant hormones belonging to the endogenous signals, which control a vast array of different processes. While auxins are growth promoting at low concentrations, higher levels are often inhibitory. Therefore, the tight contro...
Chapter
As sessile organisms, plants are often exposed to unfavorable environmental conditions (abiotic stress). Considerable losses in crop productivity all over the world are caused by abiotic stresses such as water, temperature, and salinity stresses. Phytohormones are crucial for the ability of plants to adapt to abiotic stress by mediating a wide rang...
Article
Brassica rapa auxin amidohydrolase (BrILL2) participates in the homeostasis of the plant hormones auxins by hydrolyzing the amino acid conjugates of auxins, thereby releasing the free active form of hormones. Herein, a potential role of two conserved Cys residues of BrILL2 (at sequence position 139 and 320) has been investigated by using interdisci...
Article
Full-text available
KEY MESSAGE : Stress hormones, particularly jasmonic acid, influenced root growth, auxin levels, and transcription of auxin amidohydrolase BrIAR3 in Brassica rapa seedlings, while auxin conjugate synthetases BrGH3.1 and BrGH3.9 were down-regulated by all treatments. The influence of stress hormones: jasmonic acid (JA), salicylic acid (SA), and absc...
Article
Full-text available
Salinity stress is one of the most damaging abiotic stresses to plants, causing disturbances in physiological, biochemical, and metabolic processes. The exogenous application of natural metabolites is a useful strategy to reduce the adverse effects of stress on crops. We investigated the effect of foliar application of salicylic acid (SA) and ferul...
Article
Full-text available
Brassica oleracea var. acephala is known to have a strong tolerance to low temperatures, but the protective mechanisms enabling this tolerance are unknown. Simultaneously, this species is rich in health-promoting compounds such as polyphenols, carotenoids, and glucosinolates. We hypothesize that these metabolites play an important role in the abili...
Article
Biocatalytic ring-opening of epifluorohydrin has been performed by using halohydrin dehalogenase. The enzyme from Mycobacterium sp. GP1 (HheB2) catalysed reaction with high regioselectivity and low enantioselectivity in the presence of different nucleophiles, producing racemic 1-substituted 3-fluoro-2-propanols. No by-products resulting from the ri...
Article
Full-text available
In this study cellular proteins of in vitro grown horseradish (Armoracia lapathifolia Gilib.) tissues of different morphologies, leaves (L), teratoma (TM) and tumor (TN), were separated by one-dimensional (1-DE) and two-dimensional polyacrylamide gel electrophoresis (2-DE) in order to reveal developmentally specific proteins. Moreover, proteins wer...
Article
Full-text available
Bruton's tyrosine kinase (Btk) is a member of Tec family of protein tyrosine kinases. It is expressed in B cells and myeloid cells in humans and it is crucial for maturation of B-lineage lymphoid cells. Mutations in the gene encoding human Btk result in hereditary immunodeficiency X-linked agammaglobulinemia. Sponge proteins can probably reflect ch...
Article
Full-text available
A novel activity of halohydrin dehalogenases towards spiroepoxides has been found. The enzyme from Arthrobacter sp. (HheA) catalysed highly regioselective azidolysis of spiroepoxides containing 5, 6 and 7-membered cycloalkane rings, while the enzyme from Agrobacterium radiobacter (HheC), besides high regioselectivity, also displayed moderate to hig...
Article
Full-text available
The reproductive development of the Christmas rose (Helleborus niger L.) is characterized by an uncommon feature in the world of flowering plants: after fertilization the white perianth becomes green and photosynthetically active and persists during fruit development. In the flowers in which fertilization was prevented by emasculation (unfertilized...

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Projects

Projects (4)
Project
In this project, an interdisciplinary team of scientists aims to conduct applied research in vulnerable sectors of agriculture, natural ecosystems, and biodiversity to develop guidelines for more successful adaptation to climate change of these two vulnerable sectors. The objectives of the applied research include: (1) Identification of vulnerable habitats and species to climate changes of Brassicaceae and Alliaceae families; (2) Conservation of populations of species more sensitive to climate change, by collecting and storing seeds of species of Brassica and Allium families, for long-term seed storage; (3) Recommendation of cultivars of kale and garlic more tolerant to drought and/or heat stress; (4) To study agro-technical measures, irrigation and sowing dates (or planting) which will result in more resilient production system; (5) Study of mechanisms and metabolites potentially responsible for species resistance to abiotic stress factors (drought and/or heat stress); (6) Development of Model farms concept in horticultural production adapted to current climate change; (7) Publication of articles in high-quality international journals; (8) Dissemination and transfer of results through the public media and modern information technology’s (websites and portals, social networks).
Project
Crops from the family of Brassicaceae are commercially widely spread vegetables. Abiotic stresses such as salinity and drought are the primary causes of crop loss worldwide. Phytohormones are crucial for the plant ability to adapt to abiotic stress by mediating a wide range of adaptive responses: photosynthesis modification, increased antioxidant activities, secondary metabolites accumulation, changes in gene expression. Phytohormone auxin regulates the majority of plant stress responses through interactions with stress hormones salicylic acid (SA), abscisic acid (ABA), jasmonic acid (JA), and brassinosteroids (BRA). The aim of this proposal is to investigate the abiotic stress responses of selected Brassica crops (Brassica rapa.ssp.pekinesis,Brassica oleraceavar.capitataandBrassica oleraceavar.acephala) to drought and salinity stress through integrative research approach comprising biophysical methods of photosynthesis measurements, biochemical methods of stress diagnostics, metabolomics (plant hormones and secondary metabolites) and molecular functional research. Particular focus will be placed to the role of auxin and its interactions with stress hormones (SA, ABA, JA and BRA) in stress responses and potential mechanisms of tolerance. Phytohormones auxin, SA, JA, ABA and BRA will be analyzed by UHPLC-MS/MS. Secondary metabolites, polyphenols and glucosinolates will be measured by spectrophotometry and UHPLC-MS/MS. In order to shed light on auxin homeostasis in abiotic stress, functional research will be performed by usingArabidopsis thalianalines for genes of auxin reversible conjugation (GH3andILR). Results of hormonal analyses will be correlated with basic physiological and biochemical parameters (photosynthesis parameters, ROS level, antioxidant enzymes, proline, glutathione, protein carbonyl level). Finally, stress plant hormones will be investigated as potential biostimulator of abiotic stress tolerance by foliar spray and root treatment.