Ana Sierra Martín

Ana Sierra Martín
University of Granada | UGR · Department of Cell Biology I

PhD in Biology

About

12
Publications
1,847
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233
Citations
Citations since 2016
2 Research Items
161 Citations
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2016201720182019202020212022051015202530
2016201720182019202020212022051015202530

Publications

Publications (12)
Article
Full-text available
Microglial cell precursors located in the area of the base of the pecten and the optic nerve head (BP/ONH) start to enter the retina of quail embryos at the 7th day of incubation (E7), subsequently colonizing the entire retina by central-to-peripheral tangential migration, as previously shown by our group. The present study demonstrates a precise c...
Article
Full-text available
The role of microglia during neurodegeneration remains controversial. We investigated whether microglial cells have a neurotoxic or neuroprotective function in the retina. Retinal explants from 10-day-old mice were treated in vitro with minocycline to inhibit microglial activation, with LPS to increase microglial activation, or with liposomes loade...
Article
PURPOSE. To study the incidence of DNA damage during postnatal development of the retina and its relationship with cell death. METHODS. DNA damage in the developing postnatal retina of C57BL/6 mice was assessed by determining the amount of 8-hydroxy-2'-deoxyguanosine (8-OHdG), indicative of DNA oxidation and related to the formation of DNA single-s...
Article
Full-text available
Inducible nitric oxide synthase (iNOS), which produce large amounts of nitric oxide (NO), is induced in macrophages and microglia in response to inflammatory mediators such as LPS and cytokines. Although iNOS is mainly expressed by microglia that become activated in different pathological and experimental situations, it was recently reported that u...
Article
Microglia, the brain's innate immune cell type, are cells of mesodermal origin that populate the central nervous system (CNS) during early development. Their functions which are best characterized in the developing CNS are related to programmed cell death (PCD), a physiological process that massively affects neural cell lineages and contributes to...
Article
Organotypic cultures of retinal explants allow the detailed analysis of microglial cells in a cellular microenvironment similar to that in the in situ retina, with the advantage of easy experimental manipulation. However, the in vitro culture causes changes in the retinal cytoarchitecture and induces a microglial response that may influence the res...
Article
Full-text available
Poly(ADP-ribose) polymerase (PARP)-1 is a nuclear enzyme that transfers ADP-ribose units (PAR polymer) to nuclear proteins and has been implicated in caspase-independent cell death in different models of retinal degeneration. The involvement of PARP-1 in cell death occurring during normal postnatal development of the mouse retina was investigated....
Article
Organotypic cultures of retina explants preserve the complex cellular microenvironment of the retina and have been used as a tool to assess the biological functions of some cell types. However, studies to date have shown that microglial cells activate quickly in response to the retina explantation. In this study, microglial cells migrated and ramif...
Article
The microglial response elicited by degeneration of retinal photoreceptor cells was characterized in BALB/c mice exposed to bright light for 7 hours and then kept in complete darkness for survival times ranging from 0 hours to 10 days. Photodegeneration resulted in extensive cell death in the retina, mainly in the outer nuclear layer (ONL), where t...

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