Ana Laura MartinezNational Scientific and Technical Research Council | conicet · CCT Bahia Blanca
Ana Laura Martinez
Agronomist Engineer, PhD
Molecular characterization of sunflower downy mildew in Argentina
About
7
Publications
870
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3
Citations
Citations since 2017
Introduction
I am Doctor of Agronomy and my research field is the characterization of Plasmopara halstedii causing sunflower downy mildew in Argentina, considering aspects as the molecular genetics of the pathogen, search for resistance and fungicide tolerance
Publications
Publications (7)
Downy mildew (DM) of sunflower is a devastating disease caused by Plasmopara halstedii
(Farl.) Berl. and de Toni. In Argentina, the prevalence and incidence of DM has increased
in the last decade. The present study: i) compiles information from private and public
organisms to assess the race spectrum of DM and its evolution during the last three de...
a) Race determination of P. halstedii isolates Fig. 1. A= recovery of inoculum at field B= detail of leaf sporulation C= spore solution D= reinfection of differential set of sunflower inbred lines E= phenotyping and race determination. b) SSR analysis The isolates were analyzed by eight loci SSR. The number of alleles per locus (APL), the expected...
Plasmopara halstedii (Farl.) Berl. & De Toni causing downy mildew of sunflower is responsible for considerable economic losses worldwide. Because P. halstedii can be seed-transmitted, monitoring of seeds for pathogen contamination is important for the sunflower seed trade. The relevance of asymptomatic or latent infections as factors of disease spr...
The objective of this work was to find practical procedures to overcome methodological drawbacks encountered during studies on sunflower downy mildew. Techniques for recovering living isolates of Plasmopara halstedii from the field and for the preservation of infected leaf samples for further molecular analysis were developed. A Polymerase Chain Re...
Downy mildew caused by Plasmopara halstedii is an economically challenging disease of sunflower (Helianthus annuus) worldwide. Our purpose was to evaluate naturalized wild sunflower populations as potential sources of resistance. Three populations were selected from contrasting environments in Argentina (Buenos Aires (BRW), Córdoba (RCU) and Entre...
Desde 2013 se observa un incremento de mildiu de girasol por Plasmopara halstedii en SE de Bs As y N de Santa Fé. Esto se atribuye a cambios genéticos del patógeno que confirieron tolerancia al curasemilla metalaxyl y/o superaron algunos genes Pl de resistencia de los híbridos cultivados. Ante este problema, se ha conformado una red compuesta por e...
Questions
Questions (6)
The fragment I need to clone is 500 bp, but ligation fails systematically, competent cells are ok.
I need information about this phenomenon on breeding crops of any disease. Please any job or publication you could share with me would be very useful.
Thank you so much.
I need to do some Blast work against this database but I need User and Password for INRA Sunflower Bioinformatic Resources. Please I need to enter in the next days, thank you everybody..
Should I use a microtrome? Can anyone explain me how to prepare this tissue so that I can observe haustoria in plant cells?
I am going to design primers (for sequencing) based on effectors of this pathogen species, in order to differentiate races as well as isolates. It is possible to find polymorphisms between races using those regions?
My objective is to detect a disease (Downy Mildew) in sunflower production fields to check if DM is present in a batch. I thought of using stratified sampling because pest population is agregated in some degree in the field. I considered making a subdivision: a specific number of transects (3 or 4) per hectare and doing random sampling. What do you recommend in this case?.
I have based myself on the International Standards for Phytosanitary Measures, attached here.
Projects
Project (1)
To know the genetics of the current races of the pathogen in Argentina. Do a survey of the curent pathotypes and a molecular characterization using tools like sequencing.
