• Home
  • Ana Maria Craciun
Ana Maria Craciun

Ana Maria Craciun
Babes Boyai University, Interdisciplinary Research Institute in Bio-Nano-Sciences · Nanobiophotonics and Laser Microspectroscopy Centre

PhD

About

60
Publications
9,873
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
1,524
Citations
Introduction
•Synthesis of multifunctional polymer-coated gold nano-objects with attractive optical properties, for various application such as biosensing, bioimaging and therapeutic assays. •Investigation of intrinsic multi-photon photoluminescence of gold nanoparticles for the development of efficient label-free bioimaging and immunodetection applications •Implementation of new analysis and spectroscopic imaging methods through exploiting the performances of MicroTime200 confocal microscopy system coupled
Additional affiliations
April 2014 - September 2017
Interdisciplinary research institute on bio-nano-sciences
Position
  • Researcher
January 2012 - March 2012
Ecole normale supérieure de Lyon
Position
  • PhD Student
Description
  • Research internship - The study of fluorescence properties of hybrid gold nanoparticles using fluorescence optical microscopy and spectroscopy.
June 2010 - March 2014
Interdisciplinary research institute on bio-nano-sciences
Position
  • Research Assistant
Education
October 2009 - September 2012
Babeş-Bolyai University
Field of study
  • Multifunctional plasmonic nanoparticles for optical imaging and detection methods
October 2008 - February 2010
Babeş-Bolyai University
Field of study
  • New nanoparticles for biophotonic applications: fluorescent gold nanoshells for bioimaging and photoactive pluronic nanobeads for phototherapy

Publications

Publications (60)
Article
Full-text available
Acute myocardial infarction (AMI) is considered as one of the main causes of death, threating human lives for decades. Currently, its diagnosis relies on electrocardiography (ECG), which has been proven to be insufficient. In this context, the efficient detection of cardiac biomarkers was proposed to overcome the limitations of ECG. In particular,...
Article
Full-text available
We report here the synthesis and structural characterization of novel cationic (phenothiazinyl)vinyl-pyridinium (PVP) dyes, together with optical (absorption/emission) properties and their potential applicability as fluorescent labels. Convective heating, ultrasound irradiation and mechanochemical synthesis were considered as alternative synthetic...
Article
Gold nanoparticles are known to exhibit appealing intrinsic plasmon-modulated photoluminescence (PL) properties which can be explored in various fluorescence-based sensing applications. In this paper, we evaluate the PL of different-sized gold nanospheres (AuNSs) under one-photon excitation (1PE) and develop a sensitive homogeneous immunoassay for...
Article
Photoluminescent gold nanoclusters have attracted an extensive research interest in bioimaging and therapeutics due to several distinctive advantages such as high fluorescent photostability, good dispersibility, low toxicity and large Stokes shift. However, a better understanding of the correlation between optical properties in various environments...
Article
Full-text available
Here, we perform a Surface-Enhanced Fluorescence (SEF) intensity and lifetime imaging study on linear arrays of silver half-shells (LASHSs), a class of polarization-sensitive hybrid colloidal photonic–plasmonic crystal unexplored previously in SEF. By combining fluorescence lifetime imaging microscopy, scanning confocal fluorescence imaging, Raylei...
Article
In this work, we propose a novel approach to design robust microfluidic devices with integrated plasmonic transducers allowing portability, reduced analysis time through dynamic measurements and high sensitivity. Specifically, the strategy we apply involves two steps: i) the controlled deposition of gold bipyramidal nanoparticles (AuBPs) onto a fun...
Article
In this work, we present a thorough study on the evaluation of the photothermal conversion efficiencies of gold nanobipyramids (AuBPs) under irradiation by two phototherapeutic laser lines at 785 nm and 808 nm. Due to fine tunability of the longitudinal localized surface plasmon resonance (LSPR) of AuBPs along the entire biological window, AuBPs ha...
Article
Full-text available
In this work, we design new plasmonic paper-based nanoplatforms with interesting capabilities in terms of sensitivity, efficiency and reproducibility for promoting multimodal biodetection via Localized Surface Plasmon Resonance (LSPR), Surface Enhanced Raman Spectroscopy (SERS) and Metal Enhanced Fluorescence (MEF). To succeed, we exploit the uniqu...
Article
There is still a lack of available technique to follow noninvasively the intracellular processes as well to track or disentangle various signals from the therapeutic agents at the site of action in the target cells. We present here the assessment of the intracellular kinetic of Doxorubicin (DOX) and gold nanoparticles carriers (AuNPs) by mapping si...
Article
Full-text available
Paper-based platforms can be a promising choice as portable sensors due to their low-cost and facile fabrication, ease of use, high sensitivity, specificity and flexibility. By combining the qualities of these 3D platforms with the optical properties of gold nanoparticles, it is possible to create efficient nanodevices with desired biosensing funct...
Article
In this work, we demonstrate the feasibility of gold bipyramidal-shaped nanoparticles (AuBPs) to be used as active plasmonic nanoplatforms for the detection of the biotin-streptavidin interaction in aqueous solution via both Localized Surface Plasmon Resonance and Surface Enhanced Raman Scattering (LSPR/SERS). Our proof of concept exploits the prec...
Article
Nowadays, the non-linear optical effect of two-photon excited (TPE) fluorescence has recently grown in interest in recent years over other optical imaging method, due to improved 3D spatial resolution, deep penetrability and less photodamage of living organism owing to the excitation in near-infrared region (NIR). In parallel, gold nanoparticles (A...
Chapter
Environmental pollution becomes, with every passing year, one of the most serious problems because the environment is continuously exposed to multiple contamination sources coming from various industrial activities. Recent advances in the field of nanotechnology have provided genuine opportunities to develop and use novel cost-effective environment...
Article
Due to the good transparency of the human tissue in the biological spectral window, near-infrared (NIR)-dye loaded nanosystems enable more effective light-activated therapy and better contrast imaging with major impact on nanomedicine. Herein, we prepare Pluronic coated gold nanoparticles incorporating the hydrophobic NIR dye, IR780 iodide (GNP-Plu...
Article
Full-text available
Early medical diagnostic in nanomedicine requires the implementation of innovative nanosensors with highly sensitive, selective, and reliable biomarker detection abilities. In this paper, a dual Localized Surface Plasmon Resonance - Surface Enhanced Raman Scattering (LSPR- SERS) immunosensor based on a flexible three-dimensional (3D) gold (Au) nano...
Article
Ovarian cancer is a common cause of cancer death in women and is associated with the highest mortality rates of all gynaecological malignancies. Carboplatin (CBP) is the most used cytotoxic agent in the treatment of ovarian cancer. Herein, we design and assess a CBP nanotherapeutic delivery system which allows combinatorial functionalities of chemo...
Article
Full-text available
Metal and in particular noble metal nanoparticles represent a very special class of materials which can be applied as prepared or as composite materials. In most of the cases, two main properties are exploited in a vast number of publications: biocompatibility and surface plasmon resonance (SPR). For instance, these two important properties are exp...
Article
In this research paper we propose a new class of contrast agents for the detection and multimodal imaging of CD19(+) cancer lymphoblasts. The proposed agents are based on NIR responsive hollow gold-silver nanospheres conjugated with antiCD19 monoclonal antibodies and marked with Nile Blue (NB) SERS active molecules (HNS-NB-PEG-antiCD19). Proof of c...
Article
A high-end correlated spectral and imaging multianalysis, adapted for bidimensional systems, is presented here to analyze graphene oxide (GO) and reduced GO (rGO) modified with pyrene carboxylic acid (PCA). Confocal Raman mapping was used next to two-photon excited Fluorescence Lifetime Imaging Microscopy (FLIM) to characterize the distribution of...
Article
In this paper we evaluate the effect of plasmonic enhancement on the fluorescence properties of cationic (phenothiazinyl)vinyl-pyridinium (PVP) chromophore attached to polyelectrolyte-coated gold nanorods (PE-AuNRs). We provide reliable evidence for the conveniently enhancement of both one- and two-photon excited fluorescence of PVP molecules on PE...
Article
We report on the nonlinear photochemistry fabrication of three-dimensional silver (Ag) microstructures in microfluidic channels for volumetric surface-enhanced Raman scattering (3D SERS). The fabrication of high resolution 3D Ag microstructures is obtained by a two-photon induced reduction process of silver cations, which is restricted at the focal...
Article
The versatility of the surface chemistry of carbon dots (CDs) can generate high flexibility in engineering their surface functionality for a wide and precise array of applications. In respect of this, we perform for the first time the surface passivation of carboxylated carbon nanoparticles with p-phenylenediamine to prepare water soluble photolumi...
Article
Herein we described the synthesis and the structural characterization of series of metallo-meso-(phenothiazin-3yl)porphyrins containing Ni(II), Cu(II), Zn(II) or Pd(II) coordinated in the central cavity of the macrocycle and an increasing number of peripheral phenothiazine units (from one up to four). Their photophysical properties including UV-vis...
Article
The unique photoluminescent properties of carbon dots (CDs) continue to encourage a great interest in their development for a wide range of applications in energy conversion, optoelectronics or sensing. Engaging carbon dots in resonance energy transfer processes with organic dyes could enable the design of functional materials to greatly enhance th...
Article
At present, multifunctional noble metal-based nanocomposites are extensively investigated for their potential in performing cellular imaging, diagnostics and therapy by integration of unique plasmonic properties with the spectroscopic expression and therapeutic activity of appropriate drug. In this work, we report the fabrication of 3-dimensional (...
Article
Surface-enhanced Raman scattering (SERS) and surface-enhanced fluorescence (SEF) from individual plasmonic oligomers are investigated by confocal Raman micro-spectroscopy and time-resolved fluorescence microscopy coupled to steady state micro-spectroscopy. The nanoparticle (NP) oligomers are made of either ligand protected Au or Au@SiO2 core-shell...
Article
We report on the enhancement of photoluminescence in thin films of poly[2-methoxy-5-((2′-ethylhexyl)oxy)-1,4-phenylenvinylene], neat or embedded in polystyrene, upon illumination with light as a function of annealing temperature, with our data emphasizing the picture of a light-induced conformation change that leads to the altered photophysical res...
Article
Plasmonic nanostructures offer promising routes toward artificial control of the photoluminescence properties of various emitters. Here, we investigated the photoluminescence of carboxyl-functionalized CdSe/ZnS core–shell quantum dots (c-QDs) localized near gold nanorods (AuNRs) as a function of c-QDs–AuNRs distance using the cetyltrimethylammonium...
Article
This work examines the fluorescence properties of Rose Bengal (RB) molecules conjugated to cetyltrimethylammonium bromide (CTAB) coated gold nanorods (GNRs) by performing steady-state and time-resolved fluorescence measurements. We show that the quantum yield and fluorescence lifetime can be significantly modified by the electromagnetic coupling of...
Article
We show here that treatment of thin films of conjugated polymers by illumination with light leads to an increase of the intensity of their photoluminescence by up to 42%. The corresponding enhancement of absorbance was much less pronounced. We explain this significant enhancement of photoluminescence by a planarization of the conjugated polymer cha...
Article
We review our work on hybrid gold nanoparticles that are optimized for their bright fluorescence and photobleaching resistance. Our first goal in using gold nanoparticles is to load a large density of photoactive molecules onto a biocompatible nanoplatform. Our second goal is to optimize the molecule-gold nanoparticle interaction to improve the pho...
Article
In this work we introduce a new class of multifunctional photodynamic agents based on the coupling of photosensitizer molecules with noble metal nanoparticles, which can be efficiently activated under low light intensity. The favourable modification of the photophysical properties of methylene blue (MB) in MB-loaded Pluronic-nanogold hybrids (Au-PF...
Article
Full-text available
In this paper, gold nanorods’ (GNRs) interaction with different proteins (i.e. carbonic anhydrase, lysozyme, ovalbumin and bovine serum albumin (BSA)) at physiological pH is investigated using localized surface plasmon resonance (LSPR) spectroscopy.We observe that the incubation of these proteins at different concentrations with cetyltrimethylammon...
Article
The improvement of graphene derivates’ fluorescence properties is a challenging topic and very few ways were reported up to now. In this Letter we propose an easy method to enhance the fluorescence of highly reduced graphene oxide (rGO) through non-covalent binding to a molecular fluorophore, namely the riboflavin (Rb). While the fluorescence of Rb...
Article
This paper reports on the thermally induced optical and morphological changes of bare and gelatin-coated gold nanorods (AuNRs) in the temperature range between 25 and 90 °C. Under controlled heating, the optical response of bare AuNRs displays considerable modifications, well corroborated with the reshaping of the particles. Additionally employed n...
Article
Full-text available
In this work we present a simple approach for the fabrication of periodically ordered pyramidal-shaped metallic nanostructures and demonstrate their efficiency as SERS active substrates. Our method for the fabrication of the plasmonic substrate is based on nanoimprint lithography and exploits the thermal properties of two classes of polymers, therm...
Article
In this paper, we investigate the ability of the gold nanorods (GNRs) to detect some proteins and demonstrate their potential to be used as plasmonic nanobiosensors. The GNRs were synthesized by a two-step seed-mediated growth procedure at room temperature. Firstly, a seed solution of gold nanoparticles was synthesized in the presence of cetyltrime...
Article
Full-text available
A great number of works have focused their research on the synthesis, design and optical properties of gold nanoparticles for potential biological applications (bioimaging, biosensing). For this kind of application, sharp gold nanostructures appear to exhibit the more interesting features since their surface plasmon bands are very sensitive to the...
Article
Surface-enhanced Raman scattering (SERS) nano-tags are of increasing interest in biomedical research as viable alternatives to bio-imaging techniques based on semiconductor quantum dots or fluorescent molecules. In this work, we fabricate silica-coated gold nanorods (AuNRs) encoded with two molecular labels to operate as highly effective spectrosco...
Article
The integration of metal-enhanced fluorescence (MEF) and surface-enhanced Raman scattering (SERS) onto single plasmonic nanoparticles is highly desirable to boost up accuracy and sensitivity in spectroscopic investigations. In this work, we demonstrate the feasibility of performing both MEF and SERS analysis on gold nanorods (GNRs) decorated with a...
Article
We have successfully controlled the size and shape of gold nanoparticles (GNPs) through a one-step bio-assisted procedure by using bovine serum albumin (BSA) protein as both reducing and stabilizing agent. We found that the growing process of GNPs can be directly manipulated by simply controlling the BSA concentration in solution and the reaction t...
Article
Gold nanomaterials are promising objects for applications in optics and medicine because of their unique properties related to the Surface Plasmon Resonances (SPR) phenomena. It is possible to tune these properties with the control of the size and shape of the nanomaterials. Gold hollow spheres nanomaterials are an interesting concept for encapsula...
Article
Gold nanomaterials are promising objects for applications in optics and medicine because of their unique properties related to the Surface Plasmon Resonances (SPR) phenomena. It is possible to tune these properties with the control of the size and shape of the nanomaterials. Gold hollow spheres nanomaterials are an interesting concept for encapsula...
Article
One of the relevant directions that nanotechnology is taking nowadays is connected with nanomedicine and specifically related to the use of light and nanoparticles in early diagnosis and effective therapeutics of cancer. Noble-metal nanoparticles can act under laser irradiation as effective photothermal transducers for triggering localized hyperthe...
Article
Numerous research efforts are investigating the possibility of using light interactions with metallic nanoparticles to improve the fluorescence properties of nearby molecules. Few investigations have considered the encapsulation of molecules in metallic nanocavities. In this paper, we present the optical properties of new hybrid nanoparticles consi...
Article
a b s t r a c t Gold nanomaterials are promising objects for applications in optics and medicine because of their unique properties related to the Surface Plasmon Resonances (SPR) phenomena. It is possible to tune these prop-erties with the control of the size and shape of the nanomaterials. Gold hollow spheres nanomaterials are an interesting conc...
Article
In the present work, we study the adsorption of p-aminothiophenol (p-ATP) on gold nanorods (GNRs) using localized surface plasmon resonance (LSPR) and surface-enhanced Raman scattering (SERS) spectroscopies, as function of molecular concentration and pH values in solution. The LSPR spectra reveal the adsorption of p-ATP molecules onto the ends of G...
Article
The synthesis and photophysical properties of two lipophilic quadrupolar chromophores featuring anthracenyl (1) or dibromobenzene (2) were described. These two chromophores combined significant two-photon absorption cross-sections with high fluorescence quantum yield for 1 and improved singlet oxygen generation efficiency for 2, in organic solvents...
Article
There is a need to design highly sensitive plasmonic sensors which impart a good biocompatibility and optical stability to detect low levels of analytes in biological media. In this study we report the formation of chitosan-coated silver nanoparticles of triangular shape in solution by synergistic action of chitosan and trisodium citrate in the pre...

Projects

Projects (7)
Project
The project “Flexible PDMS-integrated Plasmonic Paper as Versatile Nanochip for Metal Enhanced Fluorescence Biosensing” (acronym Chip4MEF) aims to design an innovative, versatile hybrid nanochip for highly efficient Metal Enhanced Fluorescence (MEF)–based Point-of-Care (POC) detection enabling the flexibility, miniaturization, portability of the bio(nano)sensors, fast analytical evaluation, reduced costs while achieving a rapid and sensitive MEF detection. Specifically, the originality of this proposal consists in the incorporation of the Whatman paper with pre-immobilized highly efficient anisotropic gold nanobipyramids (AuBPs) or nanorods (AuNRs) in between two polydimethylsiloxane (PDMS) layers with specific configurations, engineering thus an innovative hybrid microfluidic nanochip with enhanced and well-controlled fluorescence sensing capabilities.
Project
The project entitled “Two-photon excited time-resolved photoluminescence imaging and spectroscopy studies on single polymer-stabilized gold nanoparticles towards their applicability as optical contrast agents” addresses a challenging research subject related to the intrinsic photoluminescence in plasmonic nanostructures. The project aims to offer a deeper understanding on an interesting second-order non-linear effect, that is the two-photon excited photoluminescence (TPE PL) occurring in a particular type of gold nanoparticles (AuNPs) (i.e. gold nanorods (AuNRs)), by performing optical spectroscopy and microscopy studies on AuNRs deposited on solid substrate and inserted in tissue-imitating phantoms. The main objectives of the project are: (i) to investigate the origin of intrinsic PL in single and coupled as-prepared polymer-stabilized AuNRs by performing steady-state and time-resolved TPE PL and dark-field scattering combined microscopy and spectroscopy studies on P-AuNRs immobilized on substrate, and (ii) to demonstrate the ability of the prepared P-AuNRs to perform as optical contrast agents by monitoring their TPE PL in tissue-like phantoms under non-invasive NIR excitation.