Ana Maria Raluca Gherman

Understanding cellular interaction with nanomaterials represents a subject of great interest for the validation of new diagnostic and therapeutic tools. A full characterization of a designed product includes the evaluation of its impact on specific biological systems, including the study of cell behavior as a response to that particular interaction...

Development of cocrystals through crystal engineering is a viable strategy to formulate poorly water-soluble active pharmaceutical ingredients as stable crystalline solid forms with enhanced bioavailability. This study presents a controlled cocrystallization process by cooling for the 1:1 cocrystal of Ketoconazole, an antifungal class II drug with...

Nodularin (NOD) is a potent toxin produced by Nodularia spumigena cyanobacteria. Usually, NOD co-exists with other microcystins in environmental waters, a class of cyanotoxins secreted by certain cyanobacteria species, which makes identification difficult in the case of mixed toxins. Herein we report a complete theoretical DFT-vibrational Raman cha...

Raman spectra of oxacillin (OXN), carbenicillin (CBC), and azlocillin (AZL) are reported for the first time together with their full assignment of the normal modes, as calculated using Density Functional Theory (DFT) methods with the B3LYP exchange-correlation functional coupled to the 6-31G(d) and 6-311+G(2d,p) basis sets. Molecular docking studie...

In this work, we employed Raman and Fourier-transform infrared (FTIR) spectroscopies in order to monitor in real time the exopolysaccharides (EPS) components purified from Arthrospira platensis AICB 49 (previously known as Spirulina sp.) species. Preliminary Raman and IR spectra for EPS obtained following a stepwise ethanol extraction are reported...

Surface-enhanced Raman scattering (SERS) was employed for Enterococcus faecalis detection when using three common silver-based SERS-active sols. The SERS spectra obtained for the same species were compared in terms of bands' intensities and Raman shifting. By using multivariate analysis, the spectral data were classified depending on the sample pre...

In this study, a vibrational spectral analysis of Sorafenib was assessed, along with Density Functional Theory (DFT) and molecular docking calculations, for determining its spectroscopic profile and binding affinity. Fourier-Transform Infrared (FTIR) and Raman spectroscopies were employed and a full spectroscopic characterization of Sorafenib molec...

Advanced chemometric methods, such as fuzzy c-means, a semi-supervised clustering method, and fuzzy linear discriminant analysis (FLDA), a new robust supervised classification method in combination with principal component analysis (PCA), namely PCA-FLDA, have been successfully applied for characterization and classification of bacterial species de...

So far, in some of our previous works, we have managed to rapidly (within minutes) identify and discriminate pathogens by using surface-enhanced Raman scattering (SERS) spectroscopy with a single cell sensitivity. Having a more user friendly and robust system, which could be used not only by experts, would be the next step. In order to meet our goa...

Vibrational properties and structural behavior upon ultraviolet (UV) irradiation are reported here for DNAs isolated from three serovars of Salmonella bacteria, respectively (S. enteritidis, S. infantis and S. typhimurium) by using Fourier transform infrared spectroscopy (FT-IR). The FT-IR absorbance spectra were recorded for bacterial DNAs, contro...

https://authors.elsevier.com/c/1Zl0o54JUGxOu

The allicin pleiotropic effects, which include anti-inflammatory, anti-oxidant, anti-tumoral, and antibacterial actions, were well demonstrated and correlated with various molecular pathways. The immunostimulatory mechanism of allicin has not been elucidated; however, there is a possible cytokine stimulation from immunoglobulin release caused by al...

Palmatine is a protoberberine alkaloid separated from several plants and application as an anti-inflammatory and antibacterial agent in the therapy of gastrointestinal and genitourinary disorder. Thus, the fast quantification of palmatine is important in clinic medical assays. Herein, we report simple, fast and sensitive colorimetric visualization...

Recently, the possibility of creating surface-enhanced Raman scattering (SERS)-based biosensors for rapid detection of bacteria has been widely explored. For this purpose, we used ultrasensitive SERS spectroscopy to detect and identify common bacteria’s spectral profiles in different experimental conditions: using in situ-synthesized silver colloid...

Nonculture-based tests are gaining popularity and upsurge in the diagnosis of invasive fungal infections (IFI) fostered by their main asset, the reduced analysis time, which enables a more rapid diagnosis. In this project, three different clinical isolates of relevant filamentous fungal species were discriminated by using a rapid (less than 5 min)...