Ana Paula M Fernandes- Associate Professor
- University of São Paulo
Ana Paula M Fernandes
- Associate Professor
- University of São Paulo
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85
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Publications (85)
OBJETIVO: analisar o conhecimento de alunos dos cursos de Enfermagem e Medicina de uma universidade pública a respeito de tecnologias de prevenção ao vírus da imunodeficiência adquirida (HIV) – Profilaxia Pré-Exposição (PrEP) e Profilaxia Pós-Exposição (PEP). MÉTODO: trata-se de um estudo descritivo, transversal, com abordagem quantitativa, sendo s...
Objetivo: Apresentar o processo de elaboração e refinamento do questionário da pesquisa. Metodologia: Trata-se de um roteiro semiestruturado com 23 perguntas divididas em duas seções - a análise do perfil socioeconômico dos participantes do estudo e os conhecimentos acerca das demandas e especificidades em saúde da população LGBTQIA+. O instrumento...
Background:
Sex-determined differences are rarely addressed in the management of diseases, despite well-known contrasting outcomes between female and male patients. In COVID-19 there is a remarkable disparity, with higher rates of mortality and more severe acute disease in men compared to women, who are mostly affected by long COVID-19. Furthermor...
SARS-CoV-2 infection triggers distinct patterns of disease development characterized by significant alterations in host regulatory responses. Severe cases exhibit profound lung inflammation and systemic repercussions. Remarkably, critically ill patients display a “lipid storm”, influencing the inflammatory process and tissue damage. Sphingolipids (...
SARS-CoV-2 infection triggers distinct patterns of disease development, characterized by significant alterations in host regulatory responses. Severe cases exhibit profound lung inflammation and systemic repercussions. Remarkably, critically ill patients display a "lipid storm", influencing the inflammatory process and tissue damage. Sphingolipids...
Neste trabalho, objetivou-se identificar podcasts aplicados à educação ou que pudessem ser utilizados com esta finalidade, que tivessem a pandemia da COVID-19 como temática central ou que englobassem o assunto em seus conteúdos, com vistas a sugerir o uso desta ferramenta como metodologia complementar no processo de ensino-aprendizagem. Foram ident...
COVID-19 is associated with a dysregulated immune response. Currently, several medicines are licensed for the treatment of this disease. Due to their significant role in inhibiting pro-inflammatory cytokines and lipid mediators, glucocorticoids (GCs) have attracted a great deal of attention. Similarly, the endocannabinoid (eCB) system regulates var...
COVID‐19 has a broad spectrum of clinical manifestations associated with the host immune response heterogeneity. Despite the advances in COVID‐19 research, it is still crucial to seek a panel of molecular markers that enable accurate stratification of COVID‐19 patients. Here, we performed a study that combined analysis of blood transcriptome, demog...
The non-classical histocompatibility antigen G (HLA-G) is an immune checkpoint molecule that has been implicated in viral disorders. We evaluated the plasma soluble HLA-G (sHLA-G) in 239 individuals, arranged in COVID-19 patients (n = 189) followed up at home or in a hospital, and in healthy controls (n = 50). Increased levels of sHLA-G were observ...
Introduction: Highly active antiretroviral therapy (HAART) transformed HIV from a fatal disease to a chronic one, but it has adverse effects, such as the lipodystrophy syndrome, characterized by morphological and metabolic changes, such as reduced bone mineral density (BMD), potentiating morbidities and mortality. Strength training (ST) aims to inc...
Neste trabalho, objetivou-se identificar podcasts aplicados à educação ou que pudessem ser utilizados com esta finalidade, que tivessem a pandemia da COVID-19 como temática central ou que englobassem o assunto em seus conteúdos, com vistas a sugerir o uso desta ferramenta como metodologia complementar no processo de ensino-aprendizagem. Foram ident...
Background. Adequate management of COVID-19 needs data that predict its outcomes in men and explain its sexual dimorphism. This study evaluated immunoendocrine mediators, gene expression profiles and their correlations in a large cohort of controls and COVID-19 patients with different clinical presentations. Methods. Analyses of a public databank c...
A obra intitulada “Fudamentos e práticas nas ciências da saúde vol. 2”, publicada pela Brazilian Journals Publicações de Periódicos e Editora, apresenta um conjunto de dezessete capítulos que visa abordar diversos assuntos do conhecimento da área da saúde. Logo, os capítulos apresentam os seguintes temas: análise da correlação entre a taxa de inter...
Uncontrolled inflammatory responses play a critical role in coronavirus disease (COVID-19). In this context, because the triggering-receptor expressed on myeloid cells-1 (TREM-1) is considered an intrinsic amplifier of inflammatory signals, this study investigated the role of soluble TREM-1 (sTREM-1) as a biomarker of the severity and mortality of...
Dentre os benefícios oriundos do engajamento dos acadêmicos universitários em projetos de extensão, destaca-se o fortalecimento do papel social e da cidadania, com o aprimoramento de habilidades e competências aprendidas em sua trajetória acadêmica. Este trabalho tem como objetivo relatar as experiências vividas por alunos de graduação e de pós-gra...
O livro é uma iniciativa do “Programa USP” e foi idealizado com a intenção de apresentar informações confiáveis, com base em evidências científicas, de maneira simples, para serem utilizadas por todas as pessoas interessadas nas medidas de prevenção da infecção do novo coronavírus, causador da Covid-19. Devido ao avanço e à progressão acelerada do...
Objective: to analyze posts published in a Facebook group destined to debates on Education in University Education, with the guiding question of the study: “Given of the current scenario, led by Covid-19, what are the confrontations of graduate students and
their implications for education?”. Methods: This is an observational, prospective study of...
Objetivo: Caracterizar o perfil epidemiológico da infecção pela Aids em jovens entre 15 a 34 anos, notificados com Aids e o panorama dos óbitos no período de 2007 a 2017 em um estado do nordeste do Brasil. Método: Estudo descritivo realizado por meio de levantamento na base de dados Sistema de Informação de Agravos de Notificação. A coleta ocorreu...
In the last few months, the coronavirus disease 2019 (COVID-19) pandemic has affected millions of people worldwide and has provoked an exceptional effort from the scientific community to understand the disease. Clinical evidence suggests that severe COVID-19 is associated with both dysregulation of damage tolerance caused by pulmonary immunopatholo...
Cytokine storms and hyperinflammation, potentially controlled by glucocorticoids, occur in COVID-19; the roles of lipid mediators and acetylcholine (ACh) and how glucocorticoid therapy affects their release in Covid-19 remain unclear. Blood and bronchoalveolar lavage (BAL) samples from SARS-CoV-2- and non-SARS-CoV-2-infected subjects were collected...
Introduction:
actions to bring public high school students closer to public universities are essential to facilitate the academic transition and encourage student enrollment and permanence. Such actions still present visiting students with the possibilities inherent in the qualified academic environment and provide opportunities and new thinking ab...
Com o objetivo de identificar a opinião de graduandos em enfermagem na vivência de situações acadêmico-assistenciais q requerem a exposição, a observação e o manuseio de órgãos genitais d pacientes, elaborou-se e aplicou-se um instrumento junto aos alunos d enfermagem do 3º e 5º semestres de uma escola do interior paulista, no anos de 1997 e 1998....
Introduction
Studies report that intense physical activity influences the down-regulation of immune function in athletes as well as the interaction between adipose tissue and the immune system.
Aim
This study aimed to compare the plasma soluble levels of the immune checkpoint HLA-G (sHLA-G) molecule with the fat mass and muscle mass index among 77...
Background
The uncontrolled inflammatory response plays a critical role in the novel coronavirus disease (COVID-19) and triggering receptor expressed on myeloid cells-1 (TREM-1) is thought to be intricate to inflammatory signal amplification. This study aims to investigate the association between soluble TREM-1 (sTREM-1) and COVID-19 as a prognosti...
O HIV é uma doença definida pelos danos causados ao organismo e leva a prejuízos severos ao sistema imune. Estudos sobre a doença estão cada vez mais avançados e buscam proporcionar uma vida com menores limitações e riscos aos infectados. No entanto, a Terapia Antirretroviral altamente ativa (HAART) possui efeitos adversos, dentre esses, a síndrome...
Bodybuilders are athletes who use drastic methods to achieve their goals, such as training intensity associated with high-restriction nutritional strategies and the use of exacerbated supplementation, which can compromise your health. The aim of this studywas to evaluate the consumption of macronutrients and supplements during the pre-competition p...
Objetivo: avaliar a situação vacinal e a soroconversão conferida pela vacina da hepatite B entre ingressantes do curso de graduação em enfermagem, antes do início das atividades acadêmicas teórico-práticas. Método: estudo analítico, transversal, desenvolvido com 102 participantes. Coleta realizada através de questionário estruturado autoaplicável,...
Purpose
Since cytomegalovirus retinitis (CR) is an important cause of visual impairment among AIDS patients and HLA-C alleles have been associated with AIDS disease outcome, we typed HLA-C locus in patients with AIDS exhibiting or not CR.
Methods
Three groups of individuals were studied: (i) 49 patients with AIDS and CR (Group I), (ii) 161 patient...
OBJECTIVE: to report an experience of guided tour of high school students of public schools in the city of Ribeirão Preto and Region, through participation in the University Program for one day. METHOD: a descriptive study, type experience report, developed in the Escola de Enfermagem de Ribeirão Preto (EERP) of the Universidade de São Paulo (USP),...
Objective:
To describe the experience of the strategies and actions for insertion of activities theory-practice in the discipline of Immunology for undergraduate students in nursing.
Method:
a descriptive study, type experience reports of activities developed with students of an institution of higher education public, state of São Paulo, in the pe...
Introduction:
Expression of HLA-E molecule in the placental extravillous trophoblast is associated with immune system cell inhibition, resulting in immune tolerance to fetus during pregnancy. HIV-1 can infect trophoblast cells and modify the expression of HLA-E, which may inhibit the cytotoxic activity of the immune system.
Aim:
The aim of this...
A transmissão vertical (TV) consiste na principal forma de infecção pelo HIV-1 em menores de 13 anos e estimativas apontam que em 25% dos casos a transmissão tenha ocorrido intraútero. Nessas circunstâncias, o vírus de alguma forma ultrapassa a membrana placentária e chega ao sangue fetal. Esta revisão tem como objetivo realizar uma breve descrição...
Background/aims:
The frequency of HLA antigens/alleles associated with rapid progression from HIV infection to AIDS was evaluated in Brazilian AIDS patients with or without toxoplasmic encephalitis (TE).
Methods:
114 AIDS patients (41 with TE, 43 with anti-Toxoplasma gondii antibodies without TE, and 30 without anti-T. gondii and TE) were studie...
Introduction:
Emergent and re-emergent waterborne protozoans have become a worldwide public health problem, especially among vulnerable groups.
Methods:
This cross-sectional study evaluated 17 HIV-infected children and their families.
Results:
A high (76.5%) percentage of parasite-infected children was observed, even among children with CD4+ T...
The present study aimed evaluate the impact of strength training on bone mineral density (BMD) in individuals harboring HIV exhibiting lipodystrophy. The study included 20 subjects (16 men) aged 50.60 ± 6.40 years with reduced BMD, presenting positive serology for HIV, using HAART, and performing no regular practice of physical exercise before bein...
Background: The objective of the present study was to evaluate the impact of progressive resistance training on the body composition of HIV/AIDS-seropositive patients with lipodystrophy. Methods: The sample consisted of ten individuals with lipodystrophy submitted to a training program. Lean mass and fat mass levels were determined by double energy...
Background/aims: The frequency of HLA markers associated with rapid progression to AIDS was evaluated in Brazilian patients with AIDS exhibiting or not toxoplasmic retinochoroiditis (TRC).
Methods: 98 AIDS patients (25 with TRC, 43 with anti-T. gondii antibodies but without TCR, and 30 without anti-T. gondii antibodies and without TCR) were studie...
to identify the different types of morphological alterations from lipodystrophy syndrome (LS) in outpatients and relate them to the therapeutic regimen used.
a cross-sectional study which recruited 60 patients with HIV and LS and 79 without LS, who consented to interview and data collection from their medical notes.
the region most affected by lipo...
Under specific environmental conditions, Pseudomonas aeruginosa produces a biodegradable surfactant rhamnolipid. Evidences suggest that this biosurfactant is involved in protecting cells against oxidative stress; however, the effects of oxidative stress on its production and other virulence factors are still unclear. Here we show that rhamnolipid p...
The introduction of antiretroviral therapy has turned AIDS into a chronic disease with typical clinical manifestations of infection and the use of antiretroviral drugs, which often is accompanied by psychological disorders such as depression and panic disorder. This study aimed to characterize the quality of life of people living with HIV/AIDS, in...
Background/aims : The frequency of HLA markers associated with rapid progression to AIDS was evaluated in Brazilian patients with AIDS exhibiting or not toxoplasmic retinochoroiditis (TRC).
Methods : 98 AIDS patients (25 with TRC, 43 with anti- T . gondii antibodies but without TCR, and 30 without anti- T . gondii antibodies and without TCR) were s...
The aim of this study was to evaluate the frequency of TNFa-e microsatellites and the promoter region (TNF-308 and TNF-238) in HIV/AIDS-infected patients presenting or not lipodystrophy syndrome (LS). The design is the genetic case-control association study. Microsatellite and the TNF promoter region polymorphisms were amplified by PCR and submitte...
Cytokines play important roles in the pathogenesis of lipodystrophy syndrome (LS). Single nucleotide polymorphisms (SNPs) at positions -607(C/A) and -137(C/G) in the promoter region of the interleukin-18 (IL-18) gene and at position +874(T/A) of the interferon-gamma (IFN-gamma) gene are related to the expression of these cytokines. To examine wheth...
Lipodystrophy syndrome (LS) has been reported as visible markers that can identify HIV status. Changes in body shape are detrimental in terms of psychological welfare and may affect well-being and increase the stigma associated with HIV disease. In the current study, the psychosocial impact of LS was evaluated. A total of 84 HIV-infected patients r...
The MHC region (6p21) aggregates the major genes that contribute to susceptibility to type 1 diabetes (T1D). Three additional relevant susceptibility regions mapped on chromosomes 1p13 (PTPN22), 2q33 (CTLA-4), and 11p15 (insulin) have also been described by linkage studies. To evaluate the contribution of these susceptibility regions and the chromo...
Objective and study design:
A case-control study was conducted on 42 Brazilian women presenting with human papilloma virus (HPV) infection and cervical lesion and 87 HPV-negative women to evaluate single nucleotide polymorphisms observed in TNF-alpha, TGF-beta, IL-10, IL-6, and IFN-gamma genes.
Results and conclusion:
No significant association...
Rhamnolipids have been pointed out as promising biosurfactants. The most studied microorganisms for the aerobic production of these molecules are the bacteria of the genus Pseudomonas. The aim of this work was to produce a rhamnolipid-type biosurfactant in a bench-scale bioreactor by one strain of Pseudomonas aeruginosa isolated from oil environmen...
Rhamnolipids have been pointed out as promising biosurfactants. The most studied microorganisms for the aerobic production
of these molecules are the bacteria of the genus Pseudomonas. The aim of this work was to produce a rhamnolipid-type biosurfactant in a bench-scale bioreactor by one strain of Pseudomonas aeruginosa isolated from oil environmen...
Several side effects have been strongly associated with antiretroviral therapy in HIV patients. Among them, the lipodystrophy syndrome which presents alterations in body shape with central adipose hypertrophy and peripheral lipoatrophy, reported by patients as a visible marker identifying them as HIV patients. This manuscript presents an analysis o...
The large-scale differential gene expression in lymphomononuclear cells of six patients with recently diagnosed type), and six normal individuals matched to patients for sex and age were studied. Glass slides containing 4608 cDNAs from the IMAGE library were spotted using robotic technology. Statistical analysis was carried out by the SAM program,...
We have previously identified 30 differentially expressed genes when comparing recently diagnosed type 1 diabetes mellitus (DM-1) patients and controls paired for sex, age, and ethnic background. In this article we performed the hierarchical clustering of these genes taking into account the human-leukocyte-antigen (HLA)-DRB1/DQB1 profile. The dendr...
This descriptive study in the interior of São Paulo aimed to verify diabetes mellitus patients' knowledge about the disease, causes and complications, highlighting its importance in self care. Data were collected through interviews with 84 persons and analyzed through descriptive statistics. Average age was 53.3 +/- 13 years, time of disease 12.9 +...
Type 1 diabetes mellitus has been considered an organ-specific autoimmune disease derived from the selective destruction of pancreatic beta cells. It presents a complex pathogenesis, involving the participation of several factors, including the immunogenetic susceptibility with strong association to histocompatibility genes (HLA), environmental eve...
Infection with oncogenic human papillomavirus (HPV) is considered to be the major risk to cervical cancer. This study analyzed the influence of HPV infection on cytokine intralesional levels in cervical lesion in the presence or not of HIV infection. Cervical biopsies from 42 women were studied. HPV detection and typing were performed using amplifi...
Com enfoque em subsídios para o aperfeiçoamento de programas de enfermagem, direcionados a pacientes com aids, o presente estudo foi realizado com o objetivo de verificar a influência do comportamento sexual na sobrevida de pacientes com aids, portadores de genes associados à rápida progressão da doença. Foram entrevistados 27 pacientes com aids, g...
This study sought subsidies for improving nursing care programs for AIDS patients and aimed to verify the influence of changes in sexual behavior, including the adoption of safe sex practices, associated with the survival of AIDS patients with immunogenetic markers of rapid disease progression. 27 AIDS patients were interviewed, with genetic predis...
To evaluate the membrane expression of histocompatibility (HLA) class I (A-C) molecules on lymphomononuclear cells involved in the pathogenesis of type 1 diabetes, we studied 20 newly diagnosed Brazilian patients and 20 matched controls. The coexpression of HLA and cluster of differentiation (CD) molecules was evaluated by flow cytometry. Compared...
The progression of immunosuppression in human immunodeficiency virus (HIV)+ women has been correlated with elevated incidence of squamous intraepithelial lesions (SIL), probably indicating the role of local immune milieu. In this study, we analysed S100, and HLA class II molecule expression in cervical biopsies according to HIV status, to the sever...
To evaluate the expression of human leucocyte antigen (HLA) class II (DR and DQ) molecules on lymphomononuclear cells involved in the pathogenesis of type 1 diabetes, we studied 20 patients and 20 controls matched to patients for age, sex and HLA class II profile. The coexpression of HLA and CD3, CD4, CD8, CD19 and CD14 molecules was evaluated by f...
The acquisition of a new nomenclature and the development of molecular methods for the assignment of major histocompatibilty complex (MHC) alleles have widely contributed to the understanding of the extensive polymorphism of the system; however, many doubts have arisen to the non-specialist in terms of MHC and disease association. In order to under...
A introdução de nova nomenclatura para os componentes do complexo principal de histocompatibilidade (Major Histocompatibility Complex - MHC) e a descrição de novos métodos moleculares para a tipificação dos alelos do MHC contribuíram, grandemente, para o entendimento e identificação do extenso polimorfismo do sistema. No entanto, para o não especia...
ak5The frequency of HLA markers associated with rapid progression from HIV infection to AIDS was evaluated in Brazilian patients with AIDS exhibiting or not exhibiting cytomegalovirus retinitis (CMV-R). A total of 124 AIDS patients (44 with and 80 without CMV-R) were studied. HLA markers associated with rapid progression to AIDS were significantly...
HLA class II profile was evaluated in 64 Brazilian patients presenting with type 1 diabetes mellitus. Although the Brazilian population is highly miscegenated, HLA-DRB1*301, DRB1*04, DQB1*0302, and DQB1*0201 alleles, which are associated with the development of type 1 diabetes in several Western populations, were also overrepresented in Brazilian p...
Goyazensolide, a component extracted of Eremanthus goyazensis showed a significant inhibitory effect on egg-laying of Schistosoma mansoni during in vitro cultivation of this parasite. Motility of the worms was also reduced under treatment with goyazensolide and 90% of mortality was reached with concentrations up to 4 micrograms/ml. It has found tha...
Observation of Schistosoma mansoni oviposition during in vitro culture of adult worms for a maximum period of 10 days showed three well distinct phases in the kinetics of oviposition: an initial phase with low egg production, a period of maximum oviposition and finally a progressive reduction in the number of eggs during the late phases of culture....
Introduction: the infection with oncogenic human papillomavirus (HPV), including HPV16 and HPV18, is considered to be the major risk factor for cervical cancer development. Among HIV-positive women, studies have reported an increased prevalence and persistence of HPV16 and HPV18 explaining, in part, the increased susceptibility for squamous intraep...
Introduction: Infection with human papillomavirus 16 (HPV 16) is considered to be the major risk factor for cervical cancer development. Cytokines play an important role in the defense against viral infection. Such differences may be attributed to genetically determined molecular mechanisms including variations in transcription, translation and sec...
Memorial apresentado à Escola de Enfermagem de Ribeirão Preto da Universidade de São Paulo para o concurso de professor doutor junto ao Departamento de Enfermagem Geral e Especializada.
