Amy C. Pickering

Amy C. Pickering
The University of Edinburgh | UoE · Roslin Institute

Doctor of Philosophy
Helping scientists to develop novel therapeutics for bovine mastitis by studying the growth of Staphylococci in milk

About

23
Publications
1,673
Reads
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134
Citations
Introduction
Helping scientists to develop novel therapeutics for bovine mastitis by studying the growth and biofilm formation of Staphylococcal bacteria in milk
Additional affiliations
October 2015 - present
The Roslin Institute
Position
  • Fellow
Description
  • Host-pathogen interactions of Staphylococcus pseudintermedius and Staphylococcus aureus
September 2011 - October 2015
The University of Edinburgh
Position
  • PhD Student
Education
September 2011 - September 2015
The University of Edinburgh
Field of study
  • Microbiology
September 2007 - September 2011
Newcastle University
Field of study
  • Biomedical Sciences

Publications

Publications (23)
Article
Full-text available
Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterial pathogen responsible for significant human morbidity and mortality. Post-transcriptional regulation by small RNAs (sRNAs) has emerged as an important mechanism for controlling virulence. However, the functionality of the majority of sRNAs during infection is unknown. To address this,...
Article
Full-text available
Staphylococcus aureus is a leading cause of bovine mastitis worldwide. Despite some improved understanding of disease pathogenesis, progress towards new methods for the control of intramammary infections (IMI) has been limited, particularly in the field of vaccination. Although herd management programs have helped to reduce the number of clinical c...
Article
Full-text available
Legionella pneumophila is the most common cause of the severe respiratory infection known as Legionnaires’ disease. However, the microorganism is typically a symbiont of free-living amoeba, and our understanding of the bacterial factors that determine human pathogenicity is limited. Here we carried out a population genomic study of 902 L. pneumophi...
Article
Full-text available
The ability of some species of staphylococci to promote coagulation of plasma is a key pathogenic and diagnostic trait. Here, we provide a comprehensive analysis of the coagulase positivity of the staphylococci and its evolutionary genetic basis.
Article
Full-text available
Background: Staphylococcus aureus causes an array of diseases in both humans and livestock. Pathogenesis is mediated by a plethora of proteins secreted by S. aureus , many of which remain incompletely characterised. For example, S. aureus abundantly secretes two isoforms of the enzyme lipase into the extracellular milieu, where they scavenge upon p...
Article
Full-text available
Background: Staphylococcus aureus causes an array of diseases in both humans and livestock. Pathogenesis is mediated by a plethora of proteins secreted by S. aureus , many of which remain incompletely characterised. For example, S. aureus abundantly secretes two isoforms of the enzyme lipase into the extracellular milieu, where they scavenge upon p...
Article
Full-text available
Staphylococcus aureus is an important human and animal pathogen associated with an array of diseases, including life-threatening necrotizing pneumonia and infective endocarditis. The success of S. aureus as a pathogen has been linked in part to its ability to manipulate the host immune response through the secretion of toxins and immune evasion mol...
Article
Full-text available
Single-use plastics have often replaced more sustainable materials in microbiology laboratories. Keeping in mind that one of the objectives of the United Nations Sustainable Development Goals is responsible consumption and production, we wanted to document how many single-use plastic items could be saved by taking reduction and reuse approaches in...
Article
Full-text available
Gram-positive bacterial pathogens have an array of proteins on their cell surface that mediate interactions with the host environment. In particular, bacterial cell wall-associated (CWA) proteins play key roles in both colonization and pathogenesis. Furthermore, some CWA proteins promote specialization for host-species or mediate colonization of sp...
Preprint
Legionella pneumophila is the most common cause of the severe respiratory infection known as Legionnaires’ disease. L. pneumophila is typically a symbiont of free-living amoeba, and our understanding of the bacterial factors that determine human pathogenicity is limited. Here we carried out a population genomic study of 900 L. pneumophila isolates...
Article
Full-text available
Influenza A virus (IAV) causes annual epidemics of respiratory disease in humans, often complicated by secondary coinfection with bacterial pathogens such as Staphylococcus aureus . Here, we report that the S. aureus secreted protein lipase 1 enhances IAV replication in vitro in primary cells, including human lung fibroblasts. The proviral activity...
Article
Full-text available
Fibrinogen is an essential part of the blood coagulation cascade and a major component of the extracellular matrix in mammals. The interface between fibrinogen and bacterial pathogens is an important determinant of the outcome of infection. Here, we demonstrate that a canine host-restricted skin pathogen, Staphylococcus pseudintermedius, produces a...
Article
Full-text available
Staphylococcus pseudintermedius is the leading cause of pyoderma in dogs and is often associated with recurrent skin infections that require prolonged antibiotic therapy. High levels of antibiotic use have led to multidrug resistance including the emergence of epidemic methicillin-resistant clones. Our understanding of the pathogenesis of S. pseudi...
Article
Full-text available
Bacterial superantigens (SAgs) cause Vβ-dependent T-cell proliferation leading to immune dysregulation associated with the pathogenesis of life-threatening infections such as toxic shock syndrome, and necrotizing pneumonia. Previously, we demonstrated that staphylococcal enterotoxin-like toxin X (SElX) from Staphylococcus aureus is a classical supe...
Data
Mutations in the predicted sialic acid-binding site of SElX have limited impact on protein structure and stability. Circular dichroism analysis was performed on SElX sialic acid-binding mutants to ensure that protein structures were not affected by the mutations (i). Thermal shift assays were performed to analyse protein stability (ii). The Tm of e...
Data
Phenotypic and genetic validation of the LACΔselx mutant, LACΔselx rep and LAC selx EKQD-A strains. (i) Growth curves of S. aureus USA300 mutants (grown at 37°C for 18h) in either TSB or BHI broth. (ii) SDS-PAGE analysis of concentrated supernatant and cell wall associated (CWA) protein fractions at both mid and post exponential growth phases in BH...
Data
Analysis of SElX-binding to different lymphocyte subpopulations. (i) Flow cytometery analysis of SElX binding human neutrophils, monocytes and lymphocytes indicating the relative binding of SElX to each cell type. SElX binding was determined using a FITC conjugate mouse anti-HIS-tag antibody. (ii) Two-colour flow cytometry was used to analyse SElX-...
Data
SElX does not cause necrosis or apoptosis in human leukocytes. (i) LDH release assays were performed on human neutrophils to assess the necrotic potential of SElX compared to LukAB, employed as a positive control. Percentage lysis was determined relative to complete lysis of the neutrophils observed after addition of 0.2% (v/v) Trition-X to the cel...
Data
Neutrophil protein ligands of SElX identified by affinity precipitation analysis. (PDF)
Article
Full-text available
Large-scale recombination events have led to the emergence of epidemic clones of several major bacterial pathogens. However, the functional impact of the recombination on clonal success is not understood. Here, we identified a novel widespread hybrid clone (ST71) of livestock-associated Staphylococcus aureus that evolved from an ancestor belonging...
Article
Full-text available
In this study we investigated the cell wall-anchored fibronectin-binding proteins SpsD and SpsL from the canine commensal and pathogen Staphylococcus pseudintermedius for their role in promoting bacterial invasion into canine keratinocyte CPEK cells. Invasion was examined by the gentamicin protection assay and fluorescence microscopy. An spsD/spsL...

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