Amrutesh S Puranik

Amrutesh S Puranik
New York University | NYU · Division of Rheumatology

PhD

About

48
Publications
11,001
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1,404
Citations
Introduction
Osteopontin producing kidney-resident macrophage in lupus nephritis
Additional affiliations
January 2018 - present
NYU Langone Medical Center
Position
  • Professor (Assistant)
Description
  • Lupus nephritis and macrophages
September 2012 - April 2014
Mayo Foundation for Medical Education and Research
Position
  • PostDoc Position
September 2011 - December 2015
Mayo Clinic - Rochester
Position
  • Research Associate

Publications

Publications (48)
Article
Rationale: Macrophages regulate blood vessel structure and function in health and disease. The origins of tissue macrophages are diverse, with evidence for local production and circulatory renewal. Objective: We identified a vascular adventitial population containing macrophage progenitor cells and investigated their origins and fate. Methods a...
Article
Full-text available
Visceral adiposity is a risk factor for cardiovascular disorders, type 2 diabetes mellitus (T2D) and associated metabolic diseases. Sub-cutaneous fat is believed to be intrinsically different from visceral fat. To understand molecular mechanisms involved in metabolic advantages of fat transplantation, we studied a rat model of diet-induced adiposit...
Article
Background: Renal artery stenosis (RAS) is an important cause of chronic kidney disease and secondary hypertension. In animal models, renal ischemia leads to downregulation of growth-factor expression and loss of intrarenal microcirculation. However, little is known about the sequelae of large vessel occlusive disease on the microcirculation withi...
Article
Chronic ischemia triggers senescence in renal tubules and at least partly mediates kidney dysfunction and damage through a p16Ink4a‐related mechanism. We previously showed that mesenchymal stromal/stem cells (MSCs) delivered systemically do not effectively decrease cellular senescence in stenotic murine kidneys. We hypothesized that selective MSC t...
Article
Background: Peripheral vascular diseases may induce chronic ischemia and cellular injury distal to the arterial obstruction. Cellular senescence involves proliferation arrest in response to stress, which can damage neighboring cells. Renal artery stenosis (RAS) induces stenotic-kidney dysfunction and injury, but whether these arise from cellular se...
Article
Full-text available
Decreased NAD⁺ levels have been shown to contribute to metabolic dysfunction during aging. NAD⁺ decline can be partially prevented by knockout of the enzyme CD38. However, it is not known how CD38 is regulated during aging, and how its ecto-enzymatic activity impacts NAD⁺ homeostasis. Here we show that an increase in CD38 in white adipose tissue (W...
Article
Cell stress may give rise to insuperable growth arrest, which is defined as cellular senescence. Stenotic kidney (STK) ischemia and injury induced by renal artery stenosis (RAS) may be associated with cellular senescence. Mesenchymal stem cells (MSCs) decrease some forms of STK injury, but their ability to reverse senescence in RAS remains unknown....
Article
The ability to non-invasively detect specific damage to the kidney has been limited. Identification of extracellular vesicles released by cells, especially when under duress, might allow for monitoring and identification of specific cell types within the kidney that are stressed. We have adapted a previously published traditional flow cytometry met...
Article
Full-text available
Mesenchymal stromal/stem cells (MSC) belong to the endogenous cellular reparative system, and can be used exogenously in cell-based therapy. MSC release extracellular vesicles (EV), including exosomes and microvesicles, which mediate some of their therapeutic activity through intercellular communication. We have previously demonstrated that metabol...
Article
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Background Hypertension may be associated with renal cellular injury. Cells in distress release extracellular vesicles ( EV s), and their numbers in urine may reflect renal injury. Cellular senescence, an irreversible growth arrest in response to a noxious milieu, is characterized by release of proinflammatory cytokines. We hypothesized that EV s r...
Article
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The cellular origins of vasa vasorum are ill-defined and may involve circulating or local progenitor cells. We previously discovered that murine aortic adventitia contains Sca-1+CD45+ progenitors that produce macrophages. Here we investigated whether they are also vasculogenic. In aortas of C57BL/6 mice, Sca-1+CD45+ cells were localised to adventit...
Article
Hypertension, an important cause of chronic kidney disease, is characterized by peritubular capillary (PTC) loss. Circulating levels of endothelial microparticles (EMPs) reflect systemic endothelial injury. We hypothesized that systemic and urinary PTC-EMPs levels would reflect renal microvascular injury in hypertensive patients. We prospectively m...
Article
Full-text available
Renal artery stenosis (RAS) caused by narrowing of arteries is characterized by microvascular damage. Macrophages are implicated in repair and injury, but the specific populations responsible for these divergent roles have not been identified. Here, we characterized murine kidney F4/80 + CD64 + macrophages in three transcriptionally unique populati...
Article
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Mesenchymal stem cells (MSC) have been experimentally used for kidney repair, but modest retention limits their efficacy. Cell-surface coating allows modulating MSC homing and interaction with target cells. We coated mouse adipose tissue-derived MSC with antibodies directed against kidney injury molecule-1 (ab-KIM1), which is upregulated in injured...
Article
Full-text available
To test the hypothesis that intrinsic renal scattered tubular cells (STC-like cells) contribute to repairing injured tubular epithelial cells (TEC) by releasing extracellular vesicle (EV). EV released from primary cultured pig STC-like cells were confirmed by electron microscopy. Antimycin-A (AMA)-induced injured proximal TEC (PK1 cells) were co-cu...
Article
Full-text available
Background: Arjunarisht (AA), a formulation used as cardiotonic is a hydroalcoholic formulation of Terminalia arjuna (Roxb.) Wight and Arn. (TA) belonging to family Combretaceae. Objective: To evaluate the anti-hyperglycemic and anti-hyperlipidemic effect of Arjunarisht on high-fat diet fed animals. Materials and methods: High-fat diet fed (HF...
Article
Full-text available
Background Mesenchymal stromal/stem cell (MSC) transplantation is a promising therapy for tissue regeneration. Extracellular vesicles (EVs) released by MSCs act as their paracrine effectors by delivering proteins and genetic material to recipient cells. To assess how their cargo mediates biological processes that drive their therapeutic effects, we...
Data
Transcription factors enriched in Extracellular Vesicles (EVs). Sixteen transcription factors enriched in EVs that overlap with mRNA transcription factor targets of 4 miRNAs enriched in EVs. (PDF)
Data
Interactions between mRNA Transcription Factors (TFs). Interactions between mRNA TFs and miRNA TF target genes enriched in extracellular vesicles (EVs) using Search Tool for the Retrieval of Interacting Genes (STRING). (PDF)
Article
Mesenchymal stem/stromal cells (MSCs) have distinct capability for renal repair, but may have safety concerns. MSC-derived extracellular vesicles emerged as a novel noncellular alternative. Using a porcine model of metabolic syndrome and renal artery stenosis we tested whether extracellular vesicles attenuate renal inflammation, and if this capacit...
Article
Full-text available
Extracellular vesicles (EVs) isolated from mesenchymal stem/stromal cells (MSCs) contribute to recovery of damaged tissue. We have previously shown that porcine MSC-derived EVs transport mRNA and miRNA capable of modulating cellular pathways in recipient cells. To identify candidate factors that contribute to the therapeutic effects of porcine MSC-...
Article
Nicotinamide adenine dinucleotide (NAD) levels decrease during aging and are involved in age-related metabolic decline. To date, the mechanism responsible for the age-related reduction in NAD has not been elucidated. Here we demonstrate that expression and activity of the NADase CD38 increase with aging and that CD38 is required for the age-related...
Article
Full-text available
Aim: We evaluated the pharmacogenetic influence of genetic polymorphisms in folate pathway genes in Indian rheumatoid arthritis patients receiving methotrexate (MTX). Patients & methods: Twelve polymorphisms within nine folate pathway genes were analyzed for association with MTX response in 322 Indian rheumatoid arthritis (RA) patients and MTX p...
Article
People in developing countries have faced multigenerational undernutrition and are currently undergoing major lifestyle changes, contributing to an epidemic of metabolic diseases, though the underlying mechanisms remain unclear. Using a Wistar rat model of undernutrition over 50 generations, we show that Undernourished rats exhibit low birth-weight...
Chapter
Metabolic diseases are on the rise globally. Ayurveda identifies a large cluster of metabolic diseases as Prameha . Type 2 diabetes (T2DM) is considered as a metabolic abnormality and an advanced stage of Prameha . Therefore, this chapter deals with T2DM as a part of a cluster of metabolic diseases. International agencies have realized the complexi...
Data
Full-text available
Supplementary figures and table
Data
Full-text available
Supplementary Table 1: Hematological parameters of animals dosed with CA extracts. Supplementary Table 2: Differential white blood count of animals dosed with CA extracts. Supplementary Table 3: Serum biochemistry values of animals treated with different extracts of CA. Supplementary Figure 1: Standardization of hydroalcoholic extract of Cassia aur...
Article
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Many pharmacologically-relevant polymorphisms show variability among different populations. Though limited, data from Caucasian subjects have reported several single nucleotide polymorphism (SNPs) in folate biosynthetic pathway. These SNPs may be subjected to racial and ethnic differences. We carried out a study to determine the allelic frequencies...
Article
Full-text available
Safety pharmacology studies help in identifying preclinical adverse drug reactions. We carried out routine safety pharmacology with focus on cardiovascular variables and pharmacokinetic herb-drug interaction studies on rats fed with standardized traditional hydro-alcoholic extract and technology-based supercritical extract of Cassia auriculata for...
Article
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Azelnidipine (AZL), a long-acting dihydropyridine-based calcium antagonist, has been recently approved and used for treating ischemic heart disease and cardiac remodeling after myocardial infarction, however, its effect on hyperglycemia-induced cardiac damage has not been studied. This study examined the effect of AZL on circulating markers of card...
Article
Full-text available
The present study was conducted since there is paucity of information regarding hypoglycaemic and hypolipidemic effects of different extracts of Cassia auriculata seeds as well as their interaction with clinically used oral hypoglycaemic agent. In the present study, male adult Wistar rats were rendered hyperglycaemic by administering 60mg/kg of all...
Article
Full-text available
The present study was conducted since there is paucity of information regarding hypoglycaemic and hypolipidemic effects of different extracts of Cassia auriculata seeds as well as their interaction with clinically used oral hypoglycaemic agent. In the present study, male adult Wistar rats were rendered hyperglycaemic by administering 60mg/kg of all...

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